当前位置： SCI文献检索 > TOXICOLOGY LETTERS期刊下所有文献 > Dried blood microsamples: Suitable as an alternative matrix for the quantification of paracetamol-protein adducts?

Dried blood microsamples: Suitable as an alternative matrix for the quantification of paracetamol-protein adducts?

Abstract：

:Paracetamol (acetaminophen, APAP) is the most frequently used analgesic drug worldwide. However, patients in several specific populations can have an increased exposure to toxic APAP metabolites. Therefore, APAP-protein adducts have been proposed as an alternative marker for the assessment of APAP intoxications and as an effective tool to study and steer APAP treatment in patients with an increased risk of APAP-induced liver damage. These adducts have been determined in plasma or serum as a matrix. Blood microsampling allows the determination of a variety of analytes, including protein adducts, in a drop of blood, facilitating convenient follow-up of patients in a home-sampling context, as well as repeated sampling of pediatric patients. We therefore evaluated the use of blood-based volumetric microsamples for the quantification of APAP-protein adducts. Quantitative methods for the determination of APAP-protein adducts in dried blood and dried plasma volumetric absorptive microsamples were developed and validated. Also a preliminary evaluation of pediatric patient dried blood microsamples was conducted. Method validation encompassed the evaluation of selectivity, carry over, calibration model, accuracy and precision, matrix effect, recovery and the effect of the hematocrit on the recovery, dilution integrity, and stability. All pre-set acceptance criteria were met, except for stability. Spiking of blank blood with APAP revealed a concentration-dependent ex vivo formation of APAP-protein adducts, resulting in a response for the measurand APAP-Cys, with an apparent role for the red blood cell fraction. Analysis of authentic samples, following intake of APAP at therapeutic dosing, revealed much higher APAP-Cys concentrations in dried blood vs. dried plasma samples, making interpretation of the results in the context of published intervals difficult. In addition, in contrast to what was observed during method validation, the data obtained for the patient samples showed a high and unacceptable variation. We conclude that, for a combination of reasons, dried blood is not a suitable matrix for the quantification of APAP-protein adducts via the measurement of the APAP-Cys digestion product. The collection of plasma or serum, either in the form of a liquid sample or a dried microsample for this purpose is advised.

journal_name

Toxicol Lett

journal_title

Toxicology letters

authors

Delahaye L,Dhont E,De Cock P,De Paepe P,Stove CP

doi

10.1016/j.toxlet.2020.02.001

subject

Has Abstract

pub_date

2020-05-15 00:00:00

pages

65-74

eissn

0378-4274

issn

1879-3169

pii

S0378-4274(20)30036-9

journal_volume

324

pub_type

杂志文章

相关文献

TOXICOLOGY LETTERS文献大全
  • The mutagenicity of butadiene towards Salmonella typhimurium.

    abstract：:Gaseous butadiene (BUT) was mutagenic towards S. typhimurium strain TA 1530 when the incubation mixture was supplemented with a NADPH-fortified rat liver microsomal preparation; mutagenicity increased with the dose. A significant mutagenic effect was similarly observed when the petri dishes, containing the bacteria bu...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(80)90179-4

    authors： de Meester C,Poncelet F,Roberfroid M,Mercier M

    更新日期：1980-08-01 00:00:00

  • Dietary pyrrolizidine (Senecio) alkaloids and tissue distribution of copper and vitamin A in broiler chickens.

    abstract：:The effect of feeding a diet containing 5% tansy ragwort (TR) (Senecio jacobaea), a poisonous plant containing pyrrolizidine alkaloids (PA), on the blood and liver levels of copper, zinc, iron and vitamin A in broiler chicks was examined. Serum and liver copper and liver iron concentrations were increased in chicks fe...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(92)90017-e

    authors： Huan J,Cheeke PR,Lowry RR,Nakaue HS,Snyder SP,Whanger PD

    更新日期：1992-09-01 00:00:00

  • Effect of the cephalosporin cefepime on hepatic drug metabolising enzyme activity in rats.

    abstract：:Oral doses of the new cephalosporin cefepime administered to rats for 14 days (40 mg/kg/day) did not affect the activities of the hepatic drug metabolising enzymes measured. By contrast there were highly statistically significant increases in all measured parameters in rats treated with phenobarbitone (80 mg/kg/day), ...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(94)90199-6

    authors： Jordan MC,Waller AR,Chasseaud LF,Barbhaiya RH

    更新日期：1994-03-01 00:00:00

  • Human 3D multicellular microtissues: An upgraded model for the in vitro mechanistic investigation of inflammation-associated drug toxicity.

    abstract：:Inflammation is one of the factors that may increase the sensitivity of hepatic cells to acetaminophen (APAP) induced toxicity. To investigate the mechanisms, we exposed 3-dimensional (3D) Human Liver Microtissues, a co-culture of primary human hepatocytes (PHH) and Kupffer cells (KCs), to 0, 0.5 (low), 5 (median) and...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2019.05.004

    authors： Jiang J,Messner S,Kelm JM,van Herwijnen M,Jennen DGJ,Kleinjans JC,de Kok TM

    更新日期：2019-09-15 00:00:00

  • Acrolein and cell cycle.

    abstract：:Acrolein, a potent cilioinhibitor of cigarette smoke was tested on synchronously dividing cultures. Mitosis could be blocked whenever the addition of acrolein was made during the cell cycle but this effect was totally reversible (between 0.06 microgram/ml to 1 microgram/ml). Nucleic acid syntheses were more affected t...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(82)90043-1

    authors： Marano F,Puiseux-Dao S

    更新日期：1982-12-01 00:00:00

  • No change in serum dopamine-beta-hydroxylase activity in workers exposed to trichloroethylene.

    abstract：:To determine whether occupational exposure to trichloroethylene (TCE) influences sympathetic nerve activity, serum dopamine-beta-hydroxylase (DBH) activities were analyzed in 84 male workers exposed to TCE and 83 male age-matched controls. The workers were exposed to about 22 ppm TCE in air. There were no significant ...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(90)90187-q

    authors： Nagaya T,Ishikawa N,Hata H

    更新日期：1990-12-01 00:00:00

  • The involvement of mitochondrial apoptotic pathway in eugenol-induced cell death in human glioblastoma cells.

    abstract：:Eugenol, a natural phenolic constituent of clove oil, has a wide range of applications in medicine as a local antiseptic and anesthetic. However, the effect of eugenol on human glioblastoma is unclear. This study examined whether eugenol elevated intracellular free Ca(2+) levels ([Ca(2+)]i) and induced apoptosis in DB...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2014.10.023

    authors： Liang WZ,Chou CT,Hsu SS,Liao WC,Shieh P,Kuo DH,Tseng HW,Kuo CC,Jan CR

    更新日期：2015-01-05 00:00:00

  • Cytotoxicity of new psychoactive substances and other drugs of abuse studied in human HepG2 cells using an adopted high content screening assay.

    abstract：:New psychoactive substances (NPS) are still an emerging issue in clinical and forensic toxicology. Information about their cytotoxic potential is limited or even unavailable before distribution and thus their intake can be of high risk for consumers. The aim of the presented study was to develop a strategy to identify...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2018.11.007

    authors： Richter LHJ,Beck A,Flockerzi V,Maurer HH,Meyer MR

    更新日期：2019-02-01 00:00:00

  • The detection of S-glutathionation of hepatic carbonic anhydrase III in rats treated with paraquat or diquat.

    abstract：:Protein S-glutathionation has been demonstrated to be one of the cellular responses under oxidative stress and may be involved in many cellular metabolisms. In this study, the effect of redox cycling bipyridylium compounds, paraquat and diquat, on this protein modification was investigated. Male Sprague-Dawley rats we...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(95)03621-0

    authors： Lii CK,Wang ST,Chen HW

    更新日期：1996-02-01 00:00:00

  • Multiple signal transduction pathways alterations during nerve agent toxicity.

    abstract：:Nerve agent toxicity is primarily due to the synaptic build up of toxic levels of acetylcholine. The acute lethal effects of the nerve agents are generally attributed to respiratory failure caused by a combination of effects at both central and peripheral levels and are further complicated by copious secretions, muscl...

    journal_title：Toxicology letters

    pub_type： 杂志文章,评审

    doi：10.1016/j.toxlet.2011.09.022

    authors： RamaRao G,Bhattacharya BK

    更新日期：2012-01-05 00:00:00

  • Elimination pattern and biodistribution of [14C]chlorferron in rats.

    abstract：:Administration of [14C]chlorferron in a single oral dose of 0.5 and 20 mg/kg body weight to female rats resulted in a urinary excretion of greater than 74% of the given dose during the first 24 h. Approx. 8% of the dose was eliminated in faeces within 7 days. 7 days after dosing, very low levels of [14C]chlorferron-de...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(81)90091-6

    authors： Malik JK,Lay JP,Klein W,Korte F

    更新日期：1981-03-01 00:00:00

  • Differential neurotoxic effects of methylmercury and mercuric sulfide in rats.

    abstract：:Methylmercury (MeHg) is an environmental toxicant, while mercuric sulfide (HgS) is a main active component of cinnabar, a Chinese mineral medicine used as a sedative. Because the neurotoxicological effects of HgS were not clearly understood, in this study, we attempted to compare HgS with MeHg in various physiological...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2006.12.006

    authors： Chuu JJ,Liu SH,Lin-Shiau SY

    更新日期：2007-03-08 00:00:00

  • Progress in understanding adjuvant immunotoxicity mechanisms.

    abstract：:Over the last twenty years research has provided an important insight into the mechanisms responsible for the immunotoxicity of both local and systemic adverse reactions following the use of immunostimulating drugs and adjuvants. In this article we provide an update of the present knowledge relating to the various par...

    journal_title：Toxicology letters

    pub_type： 杂志文章,评审

    doi：10.1016/j.toxlet.2011.03.001

    authors： Batista-Duharte A,Lindblad EB,Oviedo-Orta E

    更新日期：2011-06-10 00:00:00

  • No androgenic/anti-androgenic effects of bisphenol-A in Hershberger assay using immature castrated rats.

    abstract：:Several studies have demonstrated that bisphenol A (BPA) exhibited weak estrogenic activity in the 3-day uterotrophic assay using ovariectomized (OVX) and immature rats (Toxicol. Lett. 115 (2000) 231; Regul. Toxicol. Pharmacol. 32 (2000) 118; J. Toxicol. Sci. 26 (2001) 111) and BPA also possessed anti-androgenic activ...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/s0378-4274(02)00130-3

    authors： Kim HS,Han SY,Kim TS,Kwack SJ,Lee RD,Kim IY,Seok JH,Lee BM,Yoo SD,Park KL

    更新日期：2002-09-05 00:00:00

  • Effects of amaranth on F1 generation mice.

    abstract：:The color additive, amaranth, was given in the diet to provide dietary levels of 0 (control), 0.03, 0.09 and 0.27%, from 5 weeks of age in F0 generation mice to 9 weeks of age in F1 generation mice, and some reproductive, developmental and behavioral parameters were measured. There was no effect on the parameters of l...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(92)90290-z

    authors： Tanaka T

    更新日期：1992-05-01 00:00:00

  • Subchronic oral toxicity of turmeric and ethanolic turmeric extract in female mice and rats.

    abstract：:Subchronic oral toxicity of turmeric and ethanolic turmeric extract was studied in female Swiss mice and Wistar rats fed turmeric (0, 1 and 5%) and ethanolic turmeric extract (0, 0.05 and 0.25%) through diet for 14 and/or 90 days. The administration of a high dose of turmeric (5%) for longer duration (90 days) showed ...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/s0378-4274(98)00035-6

    authors： Deshpande SS,Lalitha VS,Ingle AD,Raste AS,Gadre SG,Maru GB

    更新日期：1998-05-01 00:00:00

  • The contribution of dermal exposure to the internal exposure of bisphenol A in man.

    abstract：:New findings on Bisphenol A (BPA) contents in thermal printing papers, and receipts, in g/kg concentrations and on its dermal uptake (up to 60%) prompted us to assess the risk arising from dermal exposure. Using physiologically based toxicokinetic modelling, we simulated concentrations in blood, in liver and kidney, t...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2011.04.032

    authors： Mielke H,Partosch F,Gundert-Remy U

    更新日期：2011-07-28 00:00:00

  • The effects of aggregation on the lethality of phencyclidine in mice.

    abstract：:The lethal effects of d-amphetamine and phencyclidine (PCP) were compared when adult male mice were placed into isolated or aggregated (12 mice/cage) conditions. The LD50 of d-amphetamine decreased approx. 30-fold between isolated (87.9 mg/kg) and aggregated (2.8 mg/kg) conditions. In contrast, PCP showed only a 1.3-f...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(82)90181-3

    authors： Landauer MR,Balster RL

    更新日期：1982-07-01 00:00:00

  • Suppressive effect of 1-nitropyrene on benzo[a]pyrene-induced CYP1A1 protein expression in HepG2 cells.

    abstract：:The genotoxicity of polycyclic aromatic hydrocarbons (PAHs) and nitrated PAHs may be influenced by the interaction of the compounds. In this study, our data showed that benzo[a]pyrene (BaP)-DNA adduct levels were decreased in a dose-dependent manner when the human hepatoma cell line HepG2 simultaneously treated with B...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2005.10.002

    authors： Cherng SH,Hsu SL,Yang JL,Yu CT,Lee H

    更新日期：2006-03-01 00:00:00

  • Predicting dermal permeability of biocides in commercial cutting fluids using a LSER approach.

    abstract：:The aim of this study is to predict dermal permeability of four phenolic biocides in four different formulations using a linear solvation energy relationship (LSER) approach, with a calibrated flow through diffusion cell system. Mathematical descriptors were determined in the laboratory, by mathematical computations, ...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2007.09.005

    authors： Vijay V,Yeatts JL Jr,Riviere JE,Baynes RE

    更新日期：2007-12-10 00:00:00

  • Pb inhibits hippocampal synaptic transmission via cyclin-dependent kinase-5 dependent Synapsin 1 phosphorylation.

    abstract：:Lead (Pb) exposure impairs the nervous system, of which the injury of cognitive development is obvious. But the mechanism of Pb induced disorders of neuro-transmission remain elusive. In this study, primary hippocampal neurons were exposed to Pb at the dosage of 5 μM from days in vitro (DIV) 3 to DIV14 and the electro...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2018.08.009

    authors： Ding JJ,Zou RX,He HM,Lou ZY,Xu Y,Wang HL

    更新日期：2018-10-15 00:00:00

  • Elimination of adverse effects of ethoxyquin (EQ) by methionine hydroxy analog (MHA). Protective effects of EQ and MHA for bitterweed poisoning in sheep.

    abstract：:Dietary ethoxyquin (EQ) and methionine hydroxy analog (MHA) protected 6-8-month-old wethers from toxic doses of bitterweed (Hymenoxys odorata DC.). The EQ-MHA group received sweet feed (corn, oats, dehydrated alfalfa pellets, cane molasses and minerals), 500 g/day/sheep, supplemented with EQ and MHA (0.5% and 1.0% of ...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(83)90005-x

    authors： Kim HL,Herrig BW,Anderson AC,Jones LP,Calhoun MC

    更新日期：1983-04-01 00:00:00

  • Individual and combined toxicological effects of deoxynivalenol and zearalenone on human hepatocytes in in vitro chronic exposure conditions.

    abstract：:While numerous surveys highlighted the natural co-occurrence of mycotoxins in food, data about their toxicological combined effects is still limited. This is especially the case for chronic exposure conditions, although the latter are more representative of the mycotoxin risk associated with food consumption than acut...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2017.08.080

    authors： Smith MC,Madec S,Pawtowski A,Coton E,Hymery N

    更新日期：2017-10-05 00:00:00

  • The inhibition of HIF-2α on the ATM/Chk-2 pathway is involved in the promotion effect of arsenite on benzo(a)pyrene-induced cell transformation.

    abstract：:Both arsenite and benzo(a)pyrene (BaP) are known human carcinogens. Studies on the mode-of-action of arsenite indicate that it can also act as co-carcinogen or as a cancer promoter, and that it can facilitate progression of cancers. Some studies on development of lung cancers have suggested a synergism between arsenit...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2013.01.004

    authors： Pang Y,Xu Y,Li H,Li Y,Zhao Y,Jiang R,Shen L,Zhou J,Wang X,Liu Q

    更新日期：2013-04-12 00:00:00

  • Effects of diethylene glycol butyl ether and butoxyethoxyacetic acid on rat and human erythrocytes.

    abstract：:The toxicity of diethylene glycol butyl ether (DGBE), and its principal metabolite, butoxyethoxyacetic acid (BEAA), were assessed in vitro for rat and human red blood cells. Rat erythrocytes showed evidence of mild hemolysis when exposed to BEAA at concentrations of 5 or 10 mM for 4 h. BEAA treated rat red blood cells...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2003.09.021

    authors： Udden MM

    更新日期：2005-03-28 00:00:00

  • A dual effect of some 5-HT3 receptor antagonists on cisplatin-induced emesis in the pigeon.

    abstract：:In the present study, the emetic effect of the anticancer drug cisplatin, and protective effects of 5-HT3 receptor antagonists against cisplatin emesis were investigated in the pigeon. The experimental setting involved the i.v. administration of drugs and subsequent observation of the percentage of vomiting animals an...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(92)90256-j

    authors： Navarra P,Martire M,del Carmine R,Pozzoli G,Preziosi P

    更新日期：1992-12-01 00:00:00

  • Alpha-tocopheryl succinate protects hepatocytes from chemical-induced toxicity under physiological calcium conditions.

    abstract：:Rat and canine hepatocyte suspensions were exposed to toxic concentrations of ethyl methanesulfonate (EMS) and ionophore A-23187 in the presence and absence of extracellular calcium (Ca2+) and alpha-tocopheryl succinate (alpha-TS). The exogenous administration of alpha-TS (25 microM) completely protected hepatocytes f...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(89)90086-6

    authors： Fariss MW,Merson MH,O'Hara TM

    更新日期：1989-04-01 00:00:00

  • The therapeutic effects of Longikaurin A, a natural ent-kauranoid, in esophageal squamous cell carcinoma depend on ROS accumulation and JNK/p38 MAPK activation.

    abstract：:Effective treatments for esophageal squamous cell carcinoma (ESCC), one of the most common cancers in China, are lacking. Longikaurin A (LK-A), an ent-kauranoid diterpenoid isolated from Isodon ternifolius, has been shown to have potent cytotoxic effects on ESCC cells both in vivo and in vitro, mainly by inducing apop...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2017.08.005

    authors： Che Y,Wang J,Yuan Z,Li Y,Lu Z,Zhang Z,Zhang J,Wan J,Sun H,Chen Z,Pu J,He J

    更新日期：2017-10-05 00:00:00

  • Effect of submicron and nano-iron oxide particles on pulmonary immunity in mice.

    abstract：:Due to advances in nanotechnology, exposure to particle compounds in the workplace has become unavoidable. Assessment of their toxicity on health is an important occupational safety issue. This study was conducted in mice to investigate the toxicological effects of submicron and nano-iron oxide particles on pulmonary ...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/j.toxlet.2012.02.004

    authors： Ban M,Langonné I,Huguet N,Goutet M

    更新日期：2012-05-05 00:00:00

  • Genotoxic effects of a synthetic pyrethroid insecticide, cypermethrin, in mice in vivo.

    abstract：:The genotoxicity of cypermethrin, a synthetic pyrethroid insecticide, has been studied in vivo in mice. Bone marrow chromosome aberrations were not dose, time or route dependent, but most of the results differed significantly from controls except after 6 and 24 h of treatment using the i.p. and s.c. routes, respective...

    journal_title：Toxicology letters

    pub_type： 杂志文章

    doi：10.1016/0378-4274(88)90058-6

    authors： Bhunya SP,Pati PC

    更新日期：1988-06-01 00:00:00