Abstract:
:α-asarone is a natural phenylpropene found in several plants, which are widely used for flavoring foods and treating diseases. Previous studies have demonstrated that α-asarone has many pharmacological functions, while some reports indicated its toxicity. However, little is known about its cardiovascular effects. This study investigated developmental toxicity of α-asarone in zebrafish, especially the cardiotoxicity. Zebrafish embryos were exposed to different concentrations of α-asarone (1, 3, 5, 10, and 30 μM). Developmental toxicity assessments revealed that α-asarone did not markedly affect mortality and hatching rate. In contrast, there was a concentration-dependent increase in malformation rate of zebrafish treated with α-asarone. The most representative cardiac defects were increased heart malformation rate, pericardial edema areas, sinus venosus-bulbus arteriosus distance, and decreased heart rate. Notably, we found that α-asarone impaired the cardiac function of zebrafish by prolonging the mean QTc duration and causing T-wave abnormalities. The expressions of cardiac development-related key transcriptional regulators tbx5, nkx2.5, hand2, and gata5 were all changed under α-asarone exposure. Further investigation addressing the mechanism indicated that α-asarone triggered apoptosis mainly in the heart region of zebrafish. Moreover, the elevated expression of puma, cyto C, afap1, caspase 3, and caspase 9 in treated zebrafish suggested that mitochondrial apoptosis is likely to be the main reason for α-asarone induced cardiotoxicity. These findings revealed the cardiac developmental toxicity of α-asarone, expanding our knowledge about the toxic effect of α-asarone on living organisms.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Shang X,Ji X,Dang J,Wang L,Sun C,Liu K,Sik A,Jin Mdoi
10.1016/j.toxlet.2020.02.003subject
Has Abstractpub_date
2020-05-15 00:00:00pages
1-11eissn
0378-4274issn
1879-3169pii
S0378-4274(20)30046-1journal_volume
324pub_type
杂志文章abstract::The present study investigated the effects of in vitro methylmercury (MeHg) exposure on endogenous dopamine (DA) efflux from mouse striatal slices. MeHg produced a concentration-dependent increase in the spontaneous efflux of DA which was independent of the availability of Ca2+ in the superfusion medium. The Ca(2+)-de...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/s0378-4274(96)03787-3
更新日期:1996-12-01 00:00:00
abstract::Gaseous butadiene (BUT) was mutagenic towards S. typhimurium strain TA 1530 when the incubation mixture was supplemented with a NADPH-fortified rat liver microsomal preparation; mutagenicity increased with the dose. A significant mutagenic effect was similarly observed when the petri dishes, containing the bacteria bu...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(80)90179-4
更新日期:1980-08-01 00:00:00
abstract::The polychlorinated biphenyls (PCBs) have been demonstrated to be inducers of hepatic microsomal enzymes and some of their effects such as hormonal imbalance, and alteration of lipid and porphyrin metabolism could be ascribed to this mechanism. For this reason, the urinary excretion of D-glucaric acid (DGA), an indire...
journal_title:Toxicology letters
pub_type: 临床试验,杂志文章
doi:10.1016/s0378-4274(03)00226-1
更新日期:2003-09-15 00:00:00
abstract::Neoangiogenesis and the development of an abnormal angio-architecture in the liver are strongly linked with progressive fibrogenesis. This study aimed to evaluate the ability of curcumin to protect liver fibrosis-associated angiogenesis and capillarization of the sinusoids in experimental rats. Liver fibrosis was indu...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2013.06.240
更新日期:2013-09-12 00:00:00
abstract::Chemotherapy resistance represents a major problem for the treatment of patients with lung carcinomas. Parthenolide (PN), a naturally occurring small molecule found in herb feverfew, has been used in clinical treatment. Although its importance in treating the chemotherapy resistance has been shown, the pharmacological...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2013.06.215
更新日期:2013-08-14 00:00:00
abstract::Toluene diisocyanate (TDI), a highly reactive industrial chemical is a leading cause of occupational asthma in westernized countries. It has also been reported to be a skin sensitizer in mice and guinea pigs although instances of skin sensitivity in humans are rare. It is uncertain if skin-contact is necessary to init...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/s0378-4274(01)00325-3
更新日期:2001-04-08 00:00:00
abstract::N-cadherin has known to be involved in tumor progression and metastasis. However, it is still obscure about the signaling pathway involving in the processing of N-cadherin. Thus, we examined which signaling pathway plays a major role in the processing of N-cadherin in C6 glioma cells following treatment of cadmium (Cd...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2013.07.015
更新日期:2013-10-24 00:00:00
abstract::The purpose of this study was to evaluate the neurotoxic potential of the pesticide fipronil (FIP) towards the differentiation of mouse N2a neuroblastoma cells. At concentrations of 1, 5 and 10 μM that were not cytotoxic, as shown by two different cell viability assays, FIP impaired potently after 24h the development ...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2010.12.009
更新日期:2011-02-25 00:00:00
abstract::The elution profile of urine from rats given 63Ni i.p. using a Sephadex G-25 column was studied to investigate the chemical form and pattern of nickel (Ni) excretion. Ni was excreted probably as a mixture of complexes with chemical constituents of urine (molecular weight 200-250) within 24 h after 63Ni injection. 63Ni...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(80)90064-8
更新日期:1980-03-01 00:00:00
abstract::Central nervous system dysfunctions (most notably microencephaly and mental retardation) are among the most significant effects of in utero exposure to ethanol. Ethanol causes alterations of both neuronal and glial cells. In particular, ethanol has been shown to inhibit proliferation of astroglial cells stimulated by ...
journal_title:Toxicology letters
pub_type: 杂志文章,评审
doi:10.1016/j.toxlet.2003.12.020
更新日期:2004-04-01 00:00:00
abstract::Cytotoxicity in terms of haemolytic potency in vitro of eight surfactants failed to correlate with in vivo rabbit eye irritancy. Ability of the same surfactants to block spontaneous contractions of mouse and rabbit isolated ileum (especially the latter), correlated better with in vivo findings in rabbit eyes. Effectiv...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(83)90062-0
更新日期:1983-08-01 00:00:00
abstract::Heart rate (HR) and body core temperature (Tco) were monitored in unanesthetized, unrestrained adult Sprague-Dawley rats following intraperitoneal administration of 0, 10, 30, or 60 mg/kg of the pesticide chlordimeform (CDM). Significant non-dose-related decreases were observed in both HR and Tco in all treated groups...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(87)90208-6
更新日期:1987-02-01 00:00:00
abstract::Ambient and biological monitoring was undertaken among tobacco processors who are chronically exposed to tobacco particulates via nasopharyngeal and cutaneous routes. Ambient monitoring revealed that the inspirable dust concentration was 150-fold higher in the tobacco factory than in the control environment, and was a...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/s0378-4274(99)00097-1
更新日期:1999-09-05 00:00:00
abstract::Kahweol, the coffee-specific diterpene, has been reported to have anti-carcinogenic properties. Animal data support such a chemopreventive effect of coffee. However, the precise underlying protective mechanisms are poorly understood. In this study, the apoptotic effect of kahweol in human lung adenocarcinoma A549 cell...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2009.01.022
更新日期:2009-05-22 00:00:00
abstract::Mechanism of PAH mixtures, using granulosa tumour cells, was investigated. Cells were exposed to a mixture of all 16 priority PAHs (M1) or a mixture of five PAHs not classified as human carcinogens (M2). The effect of siAHR, siAHRR and siNFKB2 on the expression of CYP1A1, CYP1B1, GSTM1, ERα, AR and cell proliferation ...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2019.04.008
更新日期:2019-08-01 00:00:00
abstract::A computer program (Q-test) was used to evaluate the combined toxic effects of nerve agent GF and its combined form with sarin (GB/GF) in mice. Efficacy of Jielin Injection, the 2-PAM-containing antidote used successfully in China for the treatment of organophosphate pesticide poisoning, was also evaluated and compare...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/s0378-4274(97)00057-x
更新日期:1997-08-22 00:00:00
abstract::A group of 20 female albino rats was exposed to mosquito coil smoke, 8 h a day, 6 days per week, for 60 days. An additional group receiving air exposure served as control. The smoke-exposed animals had a lower body weight than the controls. Smoke-induced histopathological lesions, including an inflammation of the trac...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(87)90238-4
更新日期:1987-12-01 00:00:00
abstract::To understand the underlying mechanism for apoptosis induced by titanium dioxide nanoparticles (TNP), human airway epithelial cell line was cultured to investigate the relevant apoptosis pathways. Our results showed that the levels of reactive oxygen species and morphological apoptosis increased in a dose-dependent ma...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2010.03.014
更新日期:2010-06-16 00:00:00
abstract::Chlordecone is an organochlorine used in the 1970's as a pesticide in banana plantations. It has a long half-life in the soil and can potentially contaminate humans and animals through food. Chlordecone targets, and mainly accumulates in, the liver, leading to hepatomegaly and neurological signs in mammals. Chlordecon...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2018.09.014
更新日期:2018-12-15 00:00:00
abstract::Diclofenac is a widely prescribed NSAID that causes severe idiosyncratic drug induced liver injury (IDILI) in a small part of the patient population. Formation of protein-reactive metabolites is considered to play a role in the development of diclofenac-induced IDILI. Therefore, a high hepatic activity of enzymes invo...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2016.05.015
更新日期:2016-07-25 00:00:00
abstract::The tissue redistribution of accumulated 2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD) during infection was studied in adult male A/J mice using a common human virus coxsackievirus B3. Before infection (day 1), all mice were injected intraperitoneally (i.p.) with 1 microCi 3H-TCDD, corresponding to 0.5 microg TCDD kg(-1...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/s0378-4274(00)00217-4
更新日期:2000-07-27 00:00:00
abstract::On chronic administration of 20 microliter of chilli extract to the cheek pouch of hamsters till death, 23% of the hamsters developed shrunken eye balls and closing of the eyelids. This effect was not observed in hamsters which received a single application of the potent carcinogen methylacetoxymethyl nitrosamine (DMN...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(85)90002-5
更新日期:1985-10-01 00:00:00
abstract::The toxicity of diethylene glycol butyl ether (DGBE), and its principal metabolite, butoxyethoxyacetic acid (BEAA), were assessed in vitro for rat and human red blood cells. Rat erythrocytes showed evidence of mild hemolysis when exposed to BEAA at concentrations of 5 or 10 mM for 4 h. BEAA treated rat red blood cells...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2003.09.021
更新日期:2005-03-28 00:00:00
abstract::A number of zero tolerance provisions are contained in both food and animal feed law, e.g. for chemical substances whose occurrence is not permitted or is directly prohibited in food or animal feed. In the European Union, bans of this kind were introduced to give consumers and animals the greatest possible protection ...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2007.10.002
更新日期:2007-12-10 00:00:00
abstract::An in vivo micronucleus assay using Balb/C male mice was used to examine antigenotoxic effects of cimetidine (CM) on benzene (BZN) induced genotoxic effects. CM not only has therapeutic and immunomudolatory role, but it has also been shown to protect bone marrow stem cells from radiation induced clastogenic effects. T...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/s0378-4274(98)00138-6
更新日期:1998-09-30 00:00:00
abstract::We have studied the effects of chronic slight to moderate lead exposure on the renin-angiotensin-aldosterone system in normotensive man. Plasma renin activity (PRA), blood angiotensin I (AI) concentration, angiotensin converting enzyme (ACE) activity and aldosterone concentration were measured in 33 normotensive men, ...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(85)90096-7
更新日期:1985-04-01 00:00:00
abstract::Administration of [14C]chlorferron in a single oral dose of 0.5 and 20 mg/kg body weight to female rats resulted in a urinary excretion of greater than 74% of the given dose during the first 24 h. Approx. 8% of the dose was eliminated in faeces within 7 days. 7 days after dosing, very low levels of [14C]chlorferron-de...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(81)90091-6
更新日期:1981-03-01 00:00:00
abstract::Two-week oral administration of MDL-19,660, a triazole antidepressant compound, resulted in a dose-related thrombocytopenia in rats given 40-360 mg/kg/d and dogs treated with 5-50 mg/kg/d. Consumptive loss of platelets was not apparent since splenomegaly, hemorrhage, microscopic thrombi or prolonged coagulation times ...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(91)90098-q
更新日期:1991-04-01 00:00:00
abstract::Exposition to cadmium (Cd) has been linked to bone metabolism alterations and occurrence of osteoporosis. Despite its known renal toxicity which indirectly disrupts bone metabolism through impairment of vitamin D synthesis, increasing evidence argues for the direct action of Cd on bone-forming osteoblasts. Indeed, acc...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2012.10.006
更新日期:2012-12-17 00:00:00
abstract::Accumulation of macrophages and neutrophil granulocytes in the lung are key events in the inflammatory response to inhaled particles. The present study aims at the time course of chemotaxis in vitro in response to the challenge of various biopersistent particles and its functional relation to the transcription of infl...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2016.08.014
更新日期:2016-11-30 00:00:00