An RNAi supplemented diet as a reverse genetics tool to control bluegreen aphid, a major pest of legumes.

Abstract:

:Aphids are important agricultural pests causing major yield losses worldwide. Since aphids can rapidly develop resistance to chemical insecticides there is an urgent need to find alternative aphid pest management strategies. Despite the economic importance of bluegreen aphid (Acyrthosiphon kondoi), very few genetic resources are available to expand our current understanding and help find viable control solutions. An artificial diet is a desirable non-invasive tool to enable the functional characterisation of genes in bluegreen aphid and discover candidate target genes for future use in RNA interference (RNAi) mediated crop protection against aphids. To date no artificial diet has been developed for bluegreen aphid, so we set out to develop a suitable diet by testing and optimising existing diets. Here, we describe an artificial diet for rearing bluegreen aphid and also provide a proof of concept for the supplementation of the diet with RNAi molecules targeting the salivary gland transcript C002 and gap gene hunchback, resulting in bluegreen aphid mortality which has not yet been documented in this species. Managing this pest, for example via RNAi delivery through artificial feeding will be a major improvement to test bluegreen aphid candidate target genes for future pest control and gain significant insights into bluegreen aphid gene function.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Jacques S,Reidy-Crofts J,Sperschneider J,Kamphuis LG,Gao LL,Edwards OR,Singh KB

doi

10.1038/s41598-020-58442-4

subject

Has Abstract

pub_date

2020-01-31 00:00:00

pages

1604

issue

1

issn

2045-2322

pii

10.1038/s41598-020-58442-4

journal_volume

10

pub_type

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