Abstract:
AIMS:Brain mural cells (BMC), smooth muscle cells and pericytes, interact closely with endothelial cells and modulate numerous cerebrovascular functions. A loss of BMC function is suspected to play a role in the pathophysiology of Alzheimer's Disease (AD). METHODS:BMC markers, namely smooth muscle alpha actin (α-SMA) for smooth muscle cells, as well as platelet-derived growth factor receptor β (PDGFRβ) and aminopeptidase N (ANPEP or CD13) for pericytes, were assessed by Western immunoblotting in microvessel extracts from the parietal cortex of 60 participants of the Religious Orders study, with ages at death ranging from 75 to 98 years old. RESULTS:Participants clinically diagnosed with AD had lower vascular levels of α-SMA, PDGFRβ and CD13. These reductions were correlated with lower cognitive scores for global cognition, episodic and semantic memory, perceptual speed and visuospatial ability. In addition, α-SMA, PDGFRβ and CD13 were negatively correlated with vascular Aβ40 concentrations. Vascular levels of BMC markers were also inversely correlated with insoluble cleaved phosphorylated transactive response DNA binding protein 43 (TDP-43) (25 kDa) and positively correlated with soluble cleaved phosphorylated TDP-43 (35 kDa) in cortical homogenates, suggesting strong association between BMC loss and cleaved phosphorylated TDP-43 aggregation. CONCLUSIONS:The results of this study highlight a loss of BMC in AD. The associations between α-SMA, PDGFRβ and CD13 vascular levels with cognitive scores, TDP-43 aggregation and cerebrovascular accumulation of Aβ in the parietal cortex suggest that BMC loss contributes to both AD symptoms and pathology, further strengthening the link between cerebrovascular defects and dementia.
journal_name
Neuropathol Appl Neurobioljournal_title
Neuropathology and applied neurobiologyauthors
Bourassa P,Tremblay C,Schneider JA,Bennett DA,Calon Fdoi
10.1111/nan.12599subject
Has Abstractpub_date
2020-08-01 00:00:00pages
458-477issue
5eissn
0305-1846issn
1365-2990journal_volume
46pub_type
杂志文章abstract::Using a combined biochemical and histological approach certain conclusions can be drawn as to the origin of the increase in lysosomal enzymes in white matter from MS brains. Firstly, there is a gradient of lysosomal enzyme activity, plaque greater than periplaque greater than macroscopically normal white matter, which...
journal_title:Neuropathology and applied neurobiology
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doi:10.1111/j.1365-2990.1978.tb01357.x
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journal_title:Neuropathology and applied neurobiology
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journal_title:Neuropathology and applied neurobiology
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journal_title:Neuropathology and applied neurobiology
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doi:10.1111/j.1365-2990.1985.tb00014.x
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journal_title:Neuropathology and applied neurobiology
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doi:10.1111/j.1365-2990.1992.tb00832.x
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journal_title:Neuropathology and applied neurobiology
pub_type: 杂志文章
doi:10.1111/j.1365-2990.1984.tb00390.x
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pub_type: 杂志文章
doi:10.1111/j.1365-2990.1978.tb00548.x
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journal_title:Neuropathology and applied neurobiology
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journal_title:Neuropathology and applied neurobiology
pub_type: 杂志文章
doi:10.1111/j.1365-2990.1985.tb00039.x
更新日期:1985-11-01 00:00:00
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journal_title:Neuropathology and applied neurobiology
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journal_title:Neuropathology and applied neurobiology
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journal_title:Neuropathology and applied neurobiology
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doi:
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journal_title:Neuropathology and applied neurobiology
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journal_title:Neuropathology and applied neurobiology
pub_type: 杂志文章,评审
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journal_title:Neuropathology and applied neurobiology
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doi:10.1111/nan.12019
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journal_title:Neuropathology and applied neurobiology
pub_type: 杂志文章
doi:10.1111/j.1365-2990.2007.00799.x
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journal_title:Neuropathology and applied neurobiology
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journal_title:Neuropathology and applied neurobiology
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更新日期:1996-02-01 00:00:00
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journal_title:Neuropathology and applied neurobiology
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