Abstract:
:The liver is constantly exposed to dietary antigens, viruses, and bacterial products with inflammatory potential. For decades cellular uptake of virus has been studied in connection with infection, while the few studies designed to look into clearance mechanisms focused mainly on the role of macrophages. In recent years, attention has been directed towards the liver sinusoidal endothelial cells (LSECs), which play a central role in liver innate immunity by their ability to scavenge pathogen- and damage-associated molecular patterns. Every day our bodies are exposed to billions of gut-derived pathogens which must be efficiently removed from the circulation to prevent inflammatory and/or immune reactions in other vascular beds. Here, we have used GFP-labelled Enterobacteria phage T4 (GFP-T4-phage) as a model virus to study the viral scavenging function and metabolism in LSECs. The uptake of GFP-T4-phages was followed in real-time using deconvolution microscopy, and LSEC identity confirmed by visualization of fenestrae using structured illumination microscopy. By combining these imaging modalities with quantitative uptake and inhibition studies of radiolabelled GFP-T4-phages, we demonstrate that the bacteriophages are effectively degraded in the lysosomal compartment. Due to their high ability to take up and degrade circulating bacteriophages the LSECs may act as a primary anti-viral defence mechanism.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Øie CI,Wolfson DL,Yasunori T,Dumitriu G,Sørensen KK,McCourt PA,Ahluwalia BS,Smedsrød Bdoi
10.1038/s41598-020-57652-0subject
Has Abstractpub_date
2020-01-21 00:00:00pages
898issue
1issn
2045-2322pii
10.1038/s41598-020-57652-0journal_volume
10pub_type
杂志文章abstract::Thymol, as a dietary monoterpene, is a phenol derivative of cymene, which is the major component of the essential oil of Trachyspermum ammi (L.). It shows multiple biological activities: antifungal, antibacterial, antivirus and anti-inflammatory. T. ammi, commonly known as ajowan, belongs to Apiaceae and is an importa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31618-9
更新日期:2018-09-07 00:00:00
abstract::The Pannexin 1 (Panx1) ion and metabolite channel is expressed in a wide variety of cells where it regulates a number of cell behaviours including proliferation and differentiation. Panx1 is expressed on the cell surface as well as intracellular membranes. Previous work suggests that a region within the proximal Panx1...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46144-5
更新日期:2019-07-05 00:00:00
abstract::Recent studies suggest that glypican-1 (GPC-1) is a biomarker for prostate cancer, but there are few studies elucidating the role of GPC-1 in prostate cancer progression. We observed high expression of GPC-1 in more aggressive prostate cancer cell lines such as PC-3 and DU-145. While inhibition of GPC-1 expression in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47874-2
更新日期:2019-08-07 00:00:00
abstract::Usher Syndrome (USH) is a rare disease with hearing loss, retinitis pigmentosa and, sometimes, vestibular dysfunction. A phenotype heterogeneity is reported. Recent evidence indicates that USH is likely to belong to an emerging class of sensory ciliopathies. Olfaction has recently been implicated in ciliopathies, but ...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/srep28309
更新日期:2016-06-22 00:00:00
abstract::In this study, we aimed to disclose the association of pre- and post-stroke glycemic status with clinical outcome in patients with spontaneous intracerebral hemorrhage (sICH). It was a multicenter, prospective, observational cohort study, conducted in 13 hospitals in Beijing from January 2014 to September 2016. The as...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究
doi:10.1038/s41598-019-55610-z
更新日期:2019-12-13 00:00:00
abstract::Glucocorticoids (GC) display pleiotropic effects on the immune system. Macrophages are a major target for GC action. Here we show that dexamethasone (DEX), a synthetic GC, decreased viability of naïve bone marrow-derived macrophages (BMDM), involving an apoptotic mechanism. Administration of DEX together with lipopoly...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04323
更新日期:2014-03-10 00:00:00
abstract::Previous work has shown that human vision performs spatial integration of luminance contrast energy, where signals are squared and summed (with internal noise) over area at detection threshold. We tested that model here in an experiment using arrays of micro-pattern textures that varied in overall stimulus area and sp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29764
更新日期:2016-07-27 00:00:00
abstract::The renal sodium chloride cotransporter, NCC, in the distal convoluted tubule is important for maintaining body Na+ and K+ homeostasis. Endogenous NCC is highly ubiquitylated, but the role of individual ubiquitylation sites is not established. Here, we assessed the role of 10 ubiquitylation sites for NCC function. Tra...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12819-0
更新日期:2017-10-11 00:00:00
abstract::Global Positioning System (GPS) navigation devices and applications have become ubiquitous over the last decade. However, it is unclear whether using GPS affects our own internal navigation system, or spatial memory, which critically relies on the hippocampus. We assessed the lifetime GPS experience of 50 regular driv...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62877-0
更新日期:2020-04-14 00:00:00
abstract::Few-layer black phosphorus (BP) is the most promising material among the two-dimensional materials due to its layered structure and the excellent semiconductor properties. Currently, thin BP atomic layers are obtained mostly by mechanical exfoliation of bulk BP, which limits applications in thin-film based electronics...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23736
更新日期:2016-03-30 00:00:00
abstract::Iron overload is associated with various pathological changes which contribute to heart failure. Here, we examined mechanisms via which iron alters cardiomyocyte insulin sensitivity. Treatment of primary adult and neonatal cardiomyocytes as well as H9c2 cells with iron decreased insulin sensitivity determined via West...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41111-6
更新日期:2019-03-15 00:00:00
abstract::The current study aims at characterizing the mechanisms that allow humans to entrain the mind and body to incoming rhythmic sensory inputs in real time. We addressed this unresolved issue by examining the relationship between covert neural processes and overt behavior in the context of musical rhythm. We measured temp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20612
更新日期:2016-02-05 00:00:00
abstract::Electrolyte concentration in sweat depends on environmental context and physical condition but also on the pathophysiological status. Sweat analyzers may be therefore the future way for biological survey although how sweat electrolyte composition can reflect plasma composition remains unclear. We recruited 10 healthy ...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-017-12211-y
更新日期:2017-09-18 00:00:00
abstract::Haze is a serious issue in China with increasing concerns, and understanding the factors driving decadal-scale variations in haze occurrence is relevant for government policymaking. Using a comprehensive observational haze dataset, we demonstrate notable decadal fluctuations in the number of haze days (HD) during wint...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27424
更新日期:2016-06-10 00:00:00
abstract::Genetic alterations identified in adjacent normal appearing tissue in bladder cancer patients are indicative of a field disease. Here we assessed normal urothelium transformation and intra-tumour heterogeneity (ITH) in four patients with bladder cancer. Exome sequencing identified private acquired mutations in a lymph...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11291-0
更新日期:2017-09-15 00:00:00
abstract::Biological research requires wide-field optical imaging techniques with resolution down to the nanometer scale to study the biological process in a sub-cell or single molecular level. To meet this requirement, wide-field structured illumination method (WFSIM) has been extensively studied. The resolution of WFSIM is de...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01521-w
更新日期:2017-05-02 00:00:00
abstract::There has been much interest in understanding collective dynamics in networks of brain regions due to their role in behavior and cognitive function. Here we show that a simple, homogeneous system of densely connected oscillators, representing the aggregate activity of local brain regions, can exhibit a rich variety of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22074
更新日期:2016-02-26 00:00:00
abstract::Long-term skills training is known to induce neuroplastic alterations, but it is still debated whether these changes are always modality-specific or can be supramodal components. To address this issue, we compared finger-targeted somatosensory-evoked and auditory-evoked potentials under both Go (response) and Nogo (re...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81852-x
更新日期:2021-01-26 00:00:00
abstract::Two embedded sulfur atoms in a novel [2 + 2] Schiff-base macrocyclic dinuclear Zn(II) complex were found to be easily autoxidized to the sulfone units on air exposure, and the resultant sulfone-functionalized macrocyclic complex was obtained by the post-modification strategy exhibiting enhanced antimicrobial activitie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15898-1
更新日期:2017-11-21 00:00:00
abstract::In the study, 15 K. pneumoniae strains were isolated from the mink experiencing respiratory distress in mideastern Shandong province, China, and the prevalence of K. pneumoniae in the sampled mink was 11.9% (15/126). Fourteen (93.33%) of the 15 K. pneumoniae isolates were identified as serotype K2 and hypermucoviscosi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17681-8
更新日期:2017-12-11 00:00:00
abstract::Synthetic fertilizer production is associated with a high environmental footprint, as compounds typically dissolve rapidly leaching emissions to the atmosphere or surface waters. We tested two recovered nutrients with slower release patterns, as promising alternatives for synthetic fertilizers: struvite and a commerci...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45290-0
更新日期:2019-07-02 00:00:00
abstract::Urban coach cabin is an important indoor environment for long journey, formaldehyde (HCHO) is a carcinogenic gas and damages indoor air quality of cabins. In order to control the HCHO pollution, the air samples inside cabins were analysed with a thermally desorbed gas chromatograph, and the HCHO diffusion was simulate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-57263-4
更新日期:2020-01-15 00:00:00
abstract::A dual-specificity, paralogue-free Cdc14 phosphatase was located in the nuclei of Beauveria bassiana (filamentous entomopathogen) and functionally characterized. Inactivation of cdc14 caused defective cytokinesis due to multinucleate cells formed in Δcdc14 and 89% decrease of blastospore production, followed by slower...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03086
更新日期:2013-10-30 00:00:00
abstract::Calcium regulation plays a central role in cardiac function. Several variants in the calcium channel Cav1.2 have been implicated in arrhythmic syndromes. We screened patients with Brugada syndrome, short QT syndrome, early repolarisation syndrome, and idiopathic ventricular fibrillation to determine the frequency and ...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究
doi:10.1038/s41598-018-32867-4
更新日期:2018-10-02 00:00:00
abstract::Recently, mesoporous carbon nitride (MCN) has aroused extensive interest for its potential applications in organocatalysis, photo- and electrochemistry and CO2 capture. However, further surface functionalization of MCN for advanced nanomaterials and catalysis still remains very challenging. Here we show that acidic ca...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12901
更新日期:2015-08-05 00:00:00
abstract::We propose a novel method to determine the complex amplitude of each eigenfunction composing a vibrational wavepacket of / molecular ions evolving with a ~10 fs time scale. We find that the two-dimensional spectrogram of the kinetic energy release (KER) of H(+)/D(+) fragments plotted against the time delay of the prob...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11366
更新日期:2015-06-12 00:00:00
abstract::The endocannabinoid 2-arachidonoylglycerol (2-AG) functions as a retrograde signaling molecule mediating synaptic transmission and plasticity at both inhibitory and excitatory synapses. However, little is known about whether 2-AG signaling is involved in homeostatic regulation of miniature synaptic events at excitator...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16257
更新日期:2015-11-06 00:00:00
abstract::Hepatic steatosis (HS) can exacerbate acute pancreatitis (AP). This study aimed to investigate the relation between α1-antitrypsin (AAT) and acute pancreatitis when patients have HS. Using proteomic profiling, we identified 18 differently expressed proteins pots in the serum of rats with or without HS after surgical e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17833
更新日期:2015-12-04 00:00:00
abstract::Stem cell research has yielded promising advances in regenerative medicine, but standard assays generally lack the ability to combine different cell stimulations with rapid sample processing and precise fluid control. In this work, we describe the design and fabrication of a micro-scale cell stimulator capable of simu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11800
更新日期:2015-07-02 00:00:00
abstract::Hybrid carbon films composed of graphene film and porous carbon film may give full play to the advantages of both carbon materials, and have great potential for application in energy storage and conversion devices. Unfortunately, there are very few reports on fabrication of hybrid carbon films. Here we demonstrate a s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07050
更新日期:2014-11-14 00:00:00