Abstract:
:Studies of cell fate focus on specification, but little is known about maintenance of the differentiated state. In this study, we find that the mouse tendon cell fate requires continuous maintenance in vivo and identify an essential role for TGFβ signaling in maintenance of the tendon cell fate. To examine the role of TGFβ signaling in tenocyte function the TGFβ type II receptor (Tgfbr2) was targeted in the Scleraxis-expressing cell lineage using the ScxCre deletor. Tendon development was not disrupted in mutant embryos, but shortly after birth tenocytes lost differentiation markers and reverted to a more stem/progenitor state. Viral reintroduction of Tgfbr2 to mutants prevented and even rescued tenocyte dedifferentiation suggesting a continuous and cell autonomous role for TGFβ signaling in cell fate maintenance. These results uncover the critical importance of molecular pathways that maintain the differentiated cell fate and a key role for TGFβ signaling in these processes.
journal_name
Elifejournal_title
eLifeauthors
Tan GK,Pryce BA,Stabio A,Brigande JV,Wang C,Xia Z,Tufa SF,Keene DR,Schweitzer Rdoi
10.7554/eLife.52695subject
Has Abstractpub_date
2020-01-21 00:00:00issn
2050-084Xpii
52695journal_volume
9pub_type
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