Induction of erythroferrone in healthy humans by micro-dose recombinant erythropoietin or high-altitude exposure.

Abstract:

:The erythropoietin (Epo)-erythroferrone (ERFE)-hepcidin axis coordinates erythropoiesis and iron homeostasis. While mouse studies have established that Epo-induced ERFE production represses hepcidin synthesis by inhibiting hepatic BMP/SMAD signaling, evidence for the role of ERFE in humans is limited. To investigate the role of ERFE as a physiological erythroid regulator in humans, we conducted two studies: first, 24 males received six injections of saline (placebo), recombinant Epo (rhEpo) 20 UI kg-1 (micro-dose) or 50 UI kg-1 (low-dose). Second, we quantified ERFE in 22 subjects exposed to high altitude (3800 m) for 15 hours. In the first study, total hemoglobin mass (Hbmass) increased after low- but not after micro-dose injections, when compared to placebo. Serum ERFE levels were enhanced by rhEpo, remaining higher than after placebo for 48 (micro-dose) or 72 hours (low-dose) post-injections. Conversely, hepcidin levels decreased when Epo and ERFE arose, before any changes in serum iron parameters occurred. In the second study, serum Epo and ERFE increased at high altitude. The present results demonstrate that in healthy humans ERFE responds to slightly increased Epo levels not associated with Hbmass expansion and down-regulates hepcidin in an apparently iron-independent way. Notably, ERFE flags micro-dose Epo, thus holding promise as novel anti-doping biomarker.

journal_name

Haematologica

journal_title

Haematologica

authors

Robach P,Gammella E,Recalcati S,Girelli D,Castagna A,Roustit M,Lundby C,Lundby AK,Bouzat P,Vergès S,Séchaud G,Banco P,Uhr M,Cornu C,Sallet P,Cairo G

doi

10.3324/haematol.2019.233874

subject

Has Abstract

pub_date

2021-02-01 00:00:00

pages

384-390

issue

2

eissn

0390-6078

issn

1592-8721

pii

haematol.2019.233874

journal_volume

106

pub_type

杂志文章
  • Differentiation of monoclonal gammopathy of undetermined significance and multiple myeloma using flow cytometric characteristics of plasma cells.

    abstract:BACKGROUND AND OBJECTIVES:The differential diagnosis between multiple myeloma (MM) and monoclonal gammopathy of undetermined significance (MGUS) may be uncertain in some cases; this problem is reflected by discrepancies between different classification systems with an accordance in only 2/3 of cases. We studied whether...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Sezer O,Heider U,Zavrski I,Possinger K

    更新日期:2001-08-01 00:00:00

  • Italian blood donors with anti-HBc and occult hepatitis B virus infection.

    abstract:BACKGROUND AND OBJECTIVES:Occult hepatitis B virus (HBV) infection might allow the release of viremic units into the blood supply network if blood is tested only for hepatitis B surface antigen (HBsAg). The aim of our study was to evaluate the actual prevalence, viral load and genotype of occult HBV infections among fi...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.11224

    authors: Manzini P,Girotto M,Borsotti R,Giachino O,Guaschino R,Lanteri M,Testa D,Ghiazza P,Vacchini M,Danielle F,Pizzi A,Valpreda C,Castagno F,Curti F,Magistroni P,Abate ML,Smedile A,Rizzetto M

    更新日期:2007-12-01 00:00:00

  • International reference analysis of outcomes in adults with B-precursor Ph-negative relapsed/refractory acute lymphoblastic leukemia.

    abstract::Adults with relapsed/refractory acute lymphoblastic leukemia have an unfavourable prognosis, which is influenced by disease and patient characteristics. To further evaluate these characteristics, a retrospective analysis of 1,706 adult patients with Ph-negative relapsed/refractory B-precursor acute lymphoblastic leuke...

    journal_title:Haematologica

    pub_type: 杂志文章,多中心研究

    doi:10.3324/haematol.2016.144311

    authors: Gökbuget N,Dombret H,Ribera JM,Fielding AK,Advani A,Bassan R,Chia V,Doubek M,Giebel S,Hoelzer D,Ifrah N,Katz A,Kelsh M,Martinelli G,Morgades M,O'Brien S,Rowe JM,Stieglmaier J,Wadleigh M,Kantarjian H

    更新日期:2016-12-01 00:00:00

  • Pan-phosphatidylinositol 3-kinase inhibition with buparlisib in patients with relapsed or refractory non-Hodgkin lymphoma.

    abstract::Activation of the phosphatidylinositol 3-kinase/mechanistic target of rapamycin pathway plays a role in the pathogenesis of non-Hodgkin lymphoma. This multicenter, open-label phase 2 study evaluated buparlisib (BKM120), a pan-class I phosphatidylinositol 3-kinase inhibitor, in patients with relapsed or refractory non-...

    journal_title:Haematologica

    pub_type: 临床试验,杂志文章

    doi:10.3324/haematol.2017.169656

    authors: Younes A,Salles G,Martinelli G,Bociek RG,Barrigon DC,Barca EG,Turgut M,Gerecitano J,Kong O,Pisal CB,Tavorath R,Kim WS

    更新日期:2017-12-01 00:00:00

  • Megakaryocyte volume modulates bone marrow niche properties and cell migration dynamics.

    abstract::All hematopoietic cells that develop in the bone marrow must cross the endothelial barrier to enter the blood circulation. Blood platelets, however, are released by bigger protrusions of huge progenitor cells, named megakaryocytes, and enter the blood stream as so-called proplatelets before fragmenting into mature pla...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2018.202010

    authors: Gorelashvili MG,Angay O,Hemmen K,Klaus V,Stegner D,Heinze KG

    更新日期:2020-04-01 00:00:00

  • Anticardiolipin antibody-related thrombocytopenia: persistent remission after splenectomy.

    abstract::The lupus anticoagulant (LAC) and anticardiolipin antibody (ACA) syndromes require particular therapeutic approaches: thrombotic accidents are an indication for oral anticoagulant therapy (OAT), whereas severe thrombocytopenia may require the special treatments used for immunologic thrombocytopenic purpura (ITP). We d...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Ballerini G,Gemmati D,Moratelli S,Morelli P,Serino ML

    更新日期:1995-05-01 00:00:00

  • Combined modality therapy in advanced Hodgkin's disease: a report on 218 patients with a median follow-up of eight years.

    abstract:BACKGROUND AND OBJECTIVE:This study was designed to evaluate the efficacy and toxicity of monthly alternating ABVD/MOPP compared to ABVD/OPP regimens in patients with advanced stage Hodgkin's disease (HD), as well as in early stage patients with systemic symptoms and/or bulky disease. DESIGN AND METHODS:218 patients w...

    journal_title:Haematologica

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Anselmo AP,Cavalieri E,Enrici RM,Donato V,Pescarmona E,Biagini C,Mandelli F

    更新日期:1998-07-01 00:00:00

  • Detection and prognostic value of recurrent exportin 1 mutations in tumor and cell-free circulating DNA of patients with classical Hodgkin lymphoma.

    abstract::Classical Hodgkin lymphoma is one of the most common lymphomas and shares clinical and genetic features with primary mediastinal B-cell lymphoma. In this retrospective study, we analyzed the recurrent hotspot mutation of the exportin 1 (XPO1, p.E571K) gene, previously identified in primary mediastinal B-cell lymphoma,...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2016.145102

    authors: Camus V,Stamatoullas A,Mareschal S,Viailly PJ,Sarafan-Vasseur N,Bohers E,Dubois S,Picquenot JM,Ruminy P,Maingonnat C,Bertrand P,Cornic M,Tallon-Simon V,Becker S,Veresezan L,Frebourg T,Vera P,Bastard C,Tilly H,Jardin

    更新日期:2016-09-01 00:00:00

  • Downregulation of TREM-like transcript-1 and collagen receptor α2 subunit, two novel RUNX1-targets, contributes to platelet dysfunction in familial platelet disorder with predisposition to acute myelogenous leukemia.

    abstract::Germline RUNX1 mutations lead to thrombocytopenia and platelet dysfunction in familial platelet disorder with predisposition to acute myelogenous leukemia (AML). Multiple aspects of platelet function are impaired in these patients, associated with altered expression of genes regulated by RUNX1 We aimed to identify RUN...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2018.188904

    authors: Glembotsky AC,Sliwa D,Bluteau D,Balayn N,Marin Oyarzún CP,Raimbault A,Bordas M,Droin N,Pirozhkova I,Washington V,Goette NP,Marta RF,Favier R,Raslova H,Heller PG

    更新日期:2019-06-01 00:00:00

  • Thrombolytic therapy is effective in paroxysmal nocturnal hemoglobinuria: a series of nine patients and a review of the literature.

    abstract:BACKGROUND:Thrombosis is the major risk factor for death in patients with paroxysmal nocturnal hemoglobinuria. Previous case reports indicate that venous thrombosis in patients with paroxysmal nocturnal hemoglobinuria is amenable to thrombolysis. DESIGN AND METHODS:We reviewed the outcome of thrombolytic therapy for p...

    journal_title:Haematologica

    pub_type: 杂志文章,评审

    doi:10.3324/haematol.2011.049767

    authors: Araten DJ,Notaro R,Thaler HT,Kernan N,Boulad F,Castro-Malaspina H,Small T,Scaradavou A,Magnan H,Prockop S,Chaffee S,Gonsky J,Thertulien R,Tarquini R,Luzzatto L

    更新日期:2012-03-01 00:00:00

  • MicroRNA-34b promoter hypermethylation induces CREB overexpression and contributes to myeloid transformation.

    abstract::MicroRNA-34b down-regulation in acute myeloid leukemia was previously shown to induce CREB overexpression, thereby causing leukemia proliferation in vitro and in vivo. The role of microRNA-34b and CREB in patients with myeloid malignancies has never been evaluated. We examined microRNA-34b expression and the methylati...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2012.070664

    authors: Pigazzi M,Manara E,Bresolin S,Tregnago C,Beghin A,Baron E,Giarin E,Cho EC,Masetti R,Rao DS,Sakamoto KM,Basso G

    更新日期:2013-04-01 00:00:00

  • Myeloproliferative disease in patients with a history of multiple blood donations: a report of 8 cases.

    abstract:BACKGROUND:The clonal origin of myeloproliferative disorders has been clearly demonstrated and it is known that reactive thrombocytosis occurs as a non specific response to various inflammatory or neoplastic conditions. Only a few papers have discussed the topic of myeloproliferative diseases in blood donors. MATERIAL...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Randi ML,Rossi C,Barbone E,Cavatton G,Fabris F,Girolami A

    更新日期:1994-03-01 00:00:00

  • Clonal stability of initial leukemia in a child with central nervous system relapse 7.4 years after bone marrow relapse of common acute lymphoblastic leukemic.

    abstract::Second central nervous system (CNS) relapses represent about 7.3% of subsequent recurrences of childhood acute lymphoblastic leukemia (ALL). In most children these subsequent CNS relapses occur during the first 18 months after diagnosis of the first relapse (mean 1.42 +/- 0.73 years). We present a patient who suffered...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Eckert C,Einsiedel HG,Hartmann R,von Stackelberg A,Völpel S,Guggemos A,Hanzsch N,Kawan L,Seeger K,Henze G

    更新日期:2004-07-01 00:00:00

  • Thrombotic and bleeding complications in four subpopulations of patients with essential thrombocythemia defined by c-Mpl protein expression and PRV-1 mRNA levels.

    abstract::C-Mpl and PRV-1 expression was measured in a cohort of 48 patients with essential thrombocythemia (ET). A retrospective analysis was conducted to asses whether the presence of one or both markers correlates with a higher risk of developing thromboembolic complications. In this cohort, PRV-1 overexpression was associat...

    journal_title:Haematologica

    pub_type: 信件

    doi:

    authors: Goerttler PL,März E,Johansson PL,Andreasson B,Kutti J,Moliterno AR,Marchioli R,Spivak JL,Pahl HL,MPD Research Consortium.

    更新日期:2005-06-01 00:00:00

  • Perforin gene mutations in adult-onset hemophagocytic lymphohistiocytosis.

    abstract::Perforin gene (PRF1) mutations cause the primary form of hemophagocytic lymphohistiocytosis (HLH). We report a genetic defect of PRF1 in a 62-year-old Japanese man with recurrent episodes of HLH. Sequencing of PRF1 from both peripheral blood mononuclear cells and nail clippings showed compound heterozygous mutation, i...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.11233

    authors: Nagafuji K,Nonami A,Kumano T,Kikushige Y,Yoshimoto G,Takenaka K,Shimoda K,Ohga S,Yasukawa M,Horiuchi H,Ishii E,Harada M

    更新日期:2007-07-01 00:00:00

  • Spontaneous remission of aplastic anemia: a retrospective analysis.

    abstract:BACKGROUND AND OBJECTIVES:Although acquired aplastic anemia is usually a chronic disorder, a small proportion of patients with aplastic anemia was reported to recover spontaneously without specific therapy such as allogeneic bone marrow transplantation or immunosuppressive treatment. We attempted to determine the rate ...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Lee JH,Lee JH,Shin YR,Lee JS,Kim WK,Chi HS,Park CJ,Lee KH

    更新日期:2001-09-01 00:00:00

  • Necroptosis in spontaneously-mutated hematopoietic cells induces autoimmune bone marrow failure in mice.

    abstract::Acquired aplastic anemia is an autoimmune-mediated bone marrow failure syndrome. The mechanism by which such an autoimmune reaction is initiated is unknown. Whether and how the genetic lesions detected in patients cause autoimmune bone marrow failure have not yet been determined. We found that mice with spontaneous de...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2016.151514

    authors: Xin J,Breslin P,Wei W,Li J,Gutierrez R,Cannova J,Ni A,Ng G,Schmidt R,Chen H,Parini V,Kuo PC,Kini AR,Stiff P,Zhu J,Zhang J

    更新日期:2017-02-01 00:00:00

  • Comparative evaluation of differential leukocyte counts by Coulter VCS cytometer and direct microscopic observation.

    abstract::Comparative analyses of the leukocyte differential counting were performed using a Coulter VCS Hematology Flow Cytometer and direct microscopic observation on 547 unselected individuals analyzed at the outpatient clinic of the Institute of Hematology "L. e A. Seràgnoli" of Bologna. The Coulter VCS is able to provide l...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Zaccaria A,Celso B,Raspadori D,Motta MR,Testoni N,Rizzi S

    更新日期:1990-09-01 00:00:00

  • Flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood for ruling out myelodysplastic syndromes: a diagnostic accuracy study.

    abstract::Suspicion of myelodysplastic syndromes (MDS) is one of the commonest reasons for bone marrow aspirate in elderly patients presenting with persistent peripheral blood (PB) cytopenia of unclear etiology. A PB assay that accurately rules out MDS would have major benefits. The diagnostic accuracy of the intra-individual r...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2018.202275

    authors: Raskovalova T,Berger MG,Jacob MC,Park S,Campos L,Aanei CM,Kasprzak J,Pereira B,Labarère J,Cesbron JY,Veyrat-Masson R

    更新日期:2019-12-01 00:00:00

  • Flow cytometry characterization of leukemic phase of nasal NK/T-cell lymphoma in tumor biopsies and peripheral blood.

    abstract::We report the findings of the immunophenotypic profile of three cases of nasal T/NK cell lymphoma in leukemic phase. Flow cytometry analysis was carried out using cell suspensions of tumor nasal biopsies and peripheral blood. Tumor samples were composed by a mixture of a predominant subset of medium-size true NK cytCD...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.10654

    authors: Falcão RP,Rizzatti EG,Saggioro FP,Garcia AB,Marinato AF,Rego EM

    更新日期:2007-02-01 00:00:00

  • Efficacy of central nervous system prophylaxis with stand-alone intrathecal chemotherapy in diffuse large B-cell lymphoma patients treated with anthracycline-based chemotherapy in the rituximab era: a systematic review.

    abstract::Central nervous system (CNS) relapse of diffuse large B-cell lymphoma remains uncommon but catastrophic. The benefit of standalone intrathecal prophylaxis in reducing CNS recurrence is unclear and remains controversial. No systematic review analysing the evidence for stand-alone intrathecal prophylaxis has been perfor...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2019.229948

    authors: Eyre TA,Djebbari F,Kirkwood AA,Collins GP

    更新日期:2020-07-01 00:00:00

  • Von Willebrand's disease: a novel mutation, P1824H and the incidence of R1205H defect among families with dominant quantitative von Willebrand factor deficiency.

    abstract::To date, few mutations associated with a dominant quantitative deficiency of von Willebrand factor (VWF) and a high penetrance have been reported. This phenotype was confirmed in seven unrelated families of several patients diagnosed with von Willebrand's disease out of 70 who requested genetic studies of the VWF gene...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Casaña P,Cabrera N,Haya S,Cid AR,Aznar JA

    更新日期:2006-08-01 00:00:00

  • Long-term follow up of the FL2000 study comparing CHVP-interferon to CHVP-interferon plus rituximab in follicular lymphoma.

    abstract::Anti-CD20-containing chemotherapy regimens have become the standard of care for patients with follicular lymphoma needing cytotoxic therapy. Four randomized trials demonstrated a clinical benefit for patients treated with rituximab. However, no long-term follow up (i.e. > 5 years) of these trials is yet available. Bet...

    journal_title:Haematologica

    pub_type: 杂志文章,随机对照试验

    doi:10.3324/haematol.2012.082412

    authors: Bachy E,Houot R,Morschhauser F,Sonet A,Brice P,Belhadj K,Cartron G,Audhuy B,Fermé C,Feugier P,Sebban C,Delwail V,Maisonneuve H,Le Gouill S,Lefort S,Brousse N,Foussard C,Salles G,Groupe d'Etude des Lymphomes de l'Adult

    更新日期:2013-07-01 00:00:00

  • Intensive chemotherapy with thiotepa, busulfan and cyclophosphamide and hematopoietic stem cell rescue in relapsed or refractory primary central nervous system lymphoma and intraocular lymphoma: a retrospective study of 79 cases.

    abstract:BACKGROUND:Relapsing primary central nervous system lymphoma carries a poor prognosis when treated with conventional chemotherapy with a one-year overall survival of 25-40%. Encouraging results have been shown with intensive chemotherapy followed by autologous hematopoietic stem cell rescue. We report the results of a ...

    journal_title:Haematologica

    pub_type: 临床试验,杂志文章

    doi:10.3324/haematol.2011.060434

    authors: Soussain C,Choquet S,Fourme E,Delgadillo D,Bouabdallah K,Ghesquières H,Damaj G,Dupriez B,Vargaftig J,Gonzalez A,Houillier C,Taillandier L,Hoang-Xuan K,Leblond V

    更新日期:2012-11-01 00:00:00

  • Holotranscobalamin remains unchanged during pregnancy. Longitudinal changes of cobalamins and their binding proteins during pregnancy and postpartum.

    abstract::We confirm a decrease in cobalamins during pregnancy, and report that the active part of cobalamins (holotranscobalamin, holoTC) remains unchanged. The decrease in cobalamins is explained by a decreased holohaptocorrin (holoHC), suggesting that holoTC rather than cobalamins should be used as a marker of vitamin B12 de...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.11636

    authors: Morkbak AL,Hvas AM,Milman N,Nexo E

    更新日期:2007-12-01 00:00:00

  • Primary mediastinal B-cell lymphoma: hypermutation of the BCL6 gene targets motifs different from those in diffuse large B-cell and follicular lymphomas.

    abstract:BACKGROUND AND OBJECTIVES:Somatic hypermutation of the BCL6 gene and its expression in lymphoma represent specific markers for B-cell transit through the germinal center. Thus, analysis of BCL6 may aid in clarifying the relationship between primary mediastinal B-cell lymphoma (PMBL) and other non-thymic diffuse large c...

    journal_title:Haematologica

    pub_type: 杂志文章,meta分析

    doi:

    authors: Malpeli G,Barbi S,Moore PS,Scardoni M,Chilosi M,Scarpa A,Menestrina F

    更新日期:2004-09-01 00:00:00

  • Seminested polymerase chain reaction and heteroduplex analysis detects the monoclonality of IgH rearrangement in follicular lymphoma patients with high sensitivity.

    abstract::A new method, combining seminested polymerase chain reaction (PCR) with heteroduplex analysis, was utilized to detect follicular lymphoma (FL) cells in peripheral blood. The method, based on the detection of IgH rearrangements in DNA, detected the presence of monoclonal B cells in FL patients with a high frequency. ...

    journal_title:Haematologica

    pub_type: 信件

    doi:

    authors: Oehadian A,Koide N,Mu MM,Hassan F,Yoshida T,Yokochi T

    更新日期:2005-02-01 00:00:00

  • Effectiveness of subcutaneous low-dose alemtuzumab and rituximab combination therapy for steroid-resistant chronic graft-versus-host disease.

    abstract:BACKGROUND:Chronic graft-versus-host disease is a common late complication of allogeneic hematopoietic stem cell transplantation. Corticosteroids are the standard initial treatment. Second-line treatment has not been well defined. We evaluated the effectiveness and safety of low doses of alemtuzumab plus low doses of r...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2011.054577

    authors: Gutiérrez-Aguirre CH,Cantú-Rodríguez OG,Borjas-Almaguer OD,González-Llano O,Jaime-Pérez JC,Solano-Genesta M,Gómez-Guijosa M,Mancias-Guerra C,Tarin L,Gómez-Almaguer D

    更新日期:2012-05-01 00:00:00

  • Hepcidin demonstrates a biphasic association with anemia in acute Plasmodium falciparum malaria.

    abstract::Hepcidin levels are high and iron absorption is limited in acute malaria. The mechanism(s) that regulate hepcidin secretion remain undefined. We have measured hepcidin concentration and cytokines in 100 Kenyan children with acute falciparum malaria and different degrees of anemia. Hepcidin was increased on admission a...

    journal_title:Haematologica

    pub_type: 临床试验,杂志文章

    doi:10.3324/haematol.2012.065854

    authors: Casals-Pascual C,Huang H,Lakhal-Littleton S,Thezenas ML,Kai O,Newton CR,Roberts DJ

    更新日期:2012-11-01 00:00:00

  • IL-2 in the treatment of chronic myeloid leukemia after lymphoid blast crisis: a pilot study.

    abstract::Five patients with lymphoid blastic transformation of chronic myeloid leukemia have been treated with IL2 associated with Vincristine (VCR) plus Prednisone (PDN). Our study indicates that IL2 may be employed in the management of this disease without excessive toxicity at the higher doses in hospitalized patients and a...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Meloni G,Foà R,Tosti S,Vignetti M,Gavosto F,Mandelli F

    更新日期:1990-11-01 00:00:00