Abstract:
:The wide employment of iron nanoparticles in environmental and occupational settings underlines their potential to enter the brain. Human cell-based systems are recommended as relevant models to reduce uncertainty and to improve prediction of human toxicity. This study aimed at demonstrating the in vitro differentiation of the human umbilical cord lining-derived-mesenchymal stem cells (hCL-MSCs) into neuron-like cells (hNLCs) and the benefit of using them as an ideal primary cell source of human origin for the neuronal toxicity of Fe3O4NPs (magnetite-nanoparticles). Neuron-like phenotype was confirmed by: live morphology; Nissl body staining; protein expression of different neuronal-specific markers (immunofluorescent staining), at different maturation stages (i.e., day-3-early and day-8-full differentiated), namely β-tubulin III, MAP-2, enolase (NSE), glial protein, and almost no nestin and SOX-2 expression. Synaptic makers (SYN, GAP43, and PSD95) were also expressed. Fe3O4NPs determined a concentration- and time-dependent reduction of hNLCs viability (by ATP and the Trypan Blue test). Cell density decreased (20-50%) and apoptotic effects were detected at ≥10 μg/mL in both types of differentiated hNLCs. Three-day-differentiated hNLCs were more susceptible (toxicity appeared early and lasted for up to 48 h) than 8-day-differentiated cells (delayed effects). The study demonstrated that (i) hCL-MSCs easily differentiated into neuronal-like cells; (ii) the hNCLs susceptibility to Fe3O4NPs; and (iii) human primary cultures of neurons are new in vitro model for NP evaluation.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
De Simone U,Spinillo A,Caloni F,Gribaldo L,Coccini Tdoi
10.3390/ijms21010271subject
Has Abstractpub_date
2019-12-31 00:00:00issue
1issn
1422-0067pii
ijms21010271journal_volume
21pub_type
杂志文章abstract::The growth arrest-specific transcript 5 (GAS5) is a >200-nt lncRNA molecule that regulates several cellular functions, including proliferation, apoptosis, invasion and metastasis, across different types of human cancers. Here, we reviewed the current literature on the expression of GAS5 in leukemia, cervical, breast, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21207633
更新日期:2020-10-15 00:00:00
abstract::The maintenance of ordinal cell cycle phases is a critical biological process in cancer genesis, which is a crucial target for anti-cancer drugs. As an important natural isoquinoline alkaloid from Chinese herbal medicine, Berberine (BBR) has been reported to possess anti-cancer potentiality to induce cell cycle arrest...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19020327
更新日期:2018-01-23 00:00:00
abstract::The effects of acidic polysaccharides purified from Gastrodia rhizome on blood pressure and serum lipid levels in spontaneously hypertensive rats (SHR) fed a high-fat diet were investigated. Acidic polysaccharides were purified from crude polysaccharides by DEAE-Sepharose CL-6B. Thirty-six male SHR were randomly divid...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13010698
更新日期:2012-01-01 00:00:00
abstract::Red fluorescent genetically encoded calcium indicators (GECIs) have expanded the available pallet of colors used for the visualization of neuronal calcium activity in vivo. However, their calcium-binding domain is restricted by calmodulin from metazoans. In this study, we developed red GECI, called FRCaMP, using calmo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010111
更新日期:2020-12-24 00:00:00
abstract::The p38α mitogen-activated protein kinase (MAPK) has become an attractive target for the treatment of many diseases such as rheumatoid arthritis, inflammatory bowel disease and Crohn's disease. In this paper, 3D-QSAR and molecular docking studies were performed on 59 p38α MAPK inhibitors. Comparative molecular field a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms11093357
更新日期:2010-09-17 00:00:00
abstract::Mitochondria are usually located in the cytoplasm of cells where they generate adenosine triphosphate (ATP) to empower cellular functions. However, we found circulating mitochondria in human and animal blood. Electron microscopy confirmed the presence of mitochondria in adult human blood plasma. Flow cytometry analyse...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21062122
更新日期:2020-03-19 00:00:00
abstract::Accurate prediction of the potential hepatotoxic nature of new pharmaceuticals remains highly challenging. Therefore, novel in vitro models with improved external validity are needed to investigate hepatic metabolism and timely identify any toxicity of drugs in humans. In this study, we examined the effects of diclofe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17040584
更新日期:2016-04-16 00:00:00
abstract::The application of autologous mesenchymal stem cells (MSC) for the treatment of bone defects requires two invasive procedures and several weeks of ex vivo cell expansion. To overcome these limitations, the administration of allogeneic MSC may be attractive, because they are anticipated to be immunoprivileged. Because ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19092526
更新日期:2018-08-25 00:00:00
abstract::While necroptosis has been shown to contribute to the pathogenesis of post-infarction heart failure (HF), the role of autophagy remains unclear. Likewise, linkage between these two cell death modalities has not been sufficiently investigated. HF was induced by 60-min left coronary occlusion in adult Wistar rats and he...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207782
更新日期:2020-10-21 00:00:00
abstract::We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of "100 of the world's worst invasive alien species". The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing repeats (FIASCO) metho...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12095993
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:A growing body of data indicates that the physiology of the liver is sex-hormone dependent, with some types of liver failure occurring more frequently in males, and some in females. In males, in physiological conditions, testosterone acts via androgen receptors (AR) to increase insulin receptor (IR) expressi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22031242
更新日期:2021-01-27 00:00:00
abstract::Vitamin D receptor (VDR) is present in multiple blood cells, and the hormonal form of vitamin D, 1,25-dihydroxyvitamin D (1,25D) is essential for the proper functioning of the immune system. The role of retinoic acid receptor α (RARα) in hematopoiesis is very important, as the fusion of RARα gene with PML gene initiat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18061323
更新日期:2017-06-21 00:00:00
abstract::The translocator protein (TSPO), located at the outer mitochondrial membrane (OMM), serves multiple functions and contributes to numerous processes, including cholesterol import, mitochondrial metabolism, apoptosis, cell proliferation, Ca2+ signaling, oxidative stress, and inflammation. TSPO forms a complex with the v...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20133348
更新日期:2019-07-08 00:00:00
abstract::Oxidative stress is considered to be a key factor of the pathogenesis of Parkinson's disease, a multifactorial neurodegenerative disorder characterized by reduced dopaminergic neurons in the substantia nigra pars compacta and accumulated protein aggregates. Rotenone is a worldwide-used pesticide that induces the most ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21082761
更新日期:2020-04-16 00:00:00
abstract::Previous studies in our lab revealed that chemical zinc chelation or zinc transporter 3 (ZnT3) gene deletion suppresses the clinical features and neuropathological changes associated with experimental autoimmune encephalomyelitis (EAE). In addition, although protective functions are well documented for AMP-activated p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21093375
更新日期:2020-05-10 00:00:00
abstract::Epidermal growth factor receptor (EGFR) and the mutant EGFRvIII are major focal points in current concepts of targeted cancer therapy for glioblastoma multiforme (GBM), the most malignant primary brain tumor. The receptors participate in the key processes of tumor cell invasion and tumor-related angiogenesis and their...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18061295
更新日期:2017-06-18 00:00:00
abstract::Hepatocellular carcinoma (HCC) is a deadly form of liver malignancy with limited treatment options. Amplification and/or overexpression of c-MYC is one of the most frequent genetic events in human HCC. The mammalian target of Rapamycin Complex 1 (mTORC1) is a major functional axis regulating various aspects of cellula...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21228467
更新日期:2020-11-11 00:00:00
abstract::Demanded as an essential trace element that supports cell growth and basic functions, iron can be harmful and cancerogenic though. By exchanging between its different oxidized forms, iron overload induces free radical formation, lipid peroxidation, DNA, and protein damages, leading to carcinogenesis or ferroptosis. Ir...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20010095
更新日期:2018-12-27 00:00:00
abstract::Small RNAs are essential for a variety of cellular functions. Argonaute (AGO) proteins are associated with all of the different classes of small RNAs, and are indispensable in small RNA-mediated regulatory pathways. AGO proteins have been identified in various types of stem cells in diverse species from plants and ani...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17020208
更新日期:2016-02-04 00:00:00
abstract::Cannabis sativa L. is a plant long used for its textile fibers, seed oil, and oleoresin with medicinal and psychoactive properties. It is the main source of phytocannabinoids, with over 100 compounds detected so far. In recent years, a lot of attention has been given to the main phytochemicals present in Cannabis sati...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20235905
更新日期:2019-11-25 00:00:00
abstract::Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3 and PUFA n-6, on wo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21217911
更新日期:2020-10-24 00:00:00
abstract::Friedreich's ataxia (FA) is a trinucleotide repeats expansion neurodegenerative disorder, for which no cure or approved therapies are present. In most cases, GAA trinucleotide repetitions in the first intron of the FXN gene are the genetic trigger of FA, determining a strong reduction of frataxin, a mitochondrial prot...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21030916
更新日期:2020-01-30 00:00:00
abstract::The use of platelet-rich fibrin (PRF) is investigated in ulcer management because it provides a healing milieu rich in growth factors and cytokines. Although crucial, the relevance of secondary dressings is under-researched and no data support the use of any particular dressing in preference to another. We assessed th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21020624
更新日期:2020-01-17 00:00:00
abstract::The worldwide prevalence of neurological and neurodegenerative disorders, such as depression or Alzheimer's disease, has spread extensively throughout the last decades, becoming an enormous health issue. Numerous data indicate a distinct correlation between the altered endocannabinoid signaling and different aspects o...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21124221
更新日期:2020-06-13 00:00:00
abstract::Levodopa (LD) is used to increase dopamine level for treating Parkinson's disease. The major metabolism of LD to produce dopamine is decarboxylation. In order to understand the metabolism of LD; the electronic structure of levodopa was investigated at the Density Functional DFT/B3LYP level of theory using the 6-311+G*...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13044321
更新日期:2012-01-01 00:00:00
abstract::The analysis of saliva as a diagnostic approach for systemic diseases was proposed just two decades ago, but recently great interest in the field has emerged because of its revolutionary potential as a liquid biopsy and its usefulness as a non-invasive sampling method. Multiple molecules isolated in saliva have been p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17091531
更新日期:2016-09-12 00:00:00
abstract::Objectives: Premature birth, defined as less than 37 weeks gestation, affects approximately 12% of all live births around the world. Advances in neonatal care have resulted in the increased survival of infants born prematurely. Although prematurity is a known risk factor for different cardiovascular diseases, little i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041305
更新日期:2020-02-14 00:00:00
abstract::Poly(ADP-ribose)polymerase-1 (PARP1) is a nuclear protein implicated in DNA repair, recombination, replication, and chromatin remodeling. The aim of this study was to evaluate possible differences between PARP1(-/-) and wild-type mice regarding induction and repair of DNA lesions in irradiated male germ cells. Comet a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140918078
更新日期:2013-09-04 00:00:00
abstract::The incorporation of plant residues into soil can be considered a keystone sustainability factor in improving soil structure function. However, the effects of plant residue addition on the soil microbial communities involved in biochemical cycles and abiotic stress phenomena are poorly understood. In this study, exper...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20092125
更新日期:2019-04-29 00:00:00
abstract:BACKGROUND:Psoriasis is hallmarked by vascular dysfunction, arterial hypertension, and an increased risk for cardiovascular diseases. We have shown recently that skin-driven interleukin (IL)-17A expression promotes psoriasis-like disease in mice, and this is associated with vascular inflammation, vascular dysfunction, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20174261
更新日期:2019-08-30 00:00:00