Synergistic Effects of Efflux Pump Modulators on the Azole Antifungal Susceptibility of Microsporum canis.

Abstract:

:The microbiologic and clinical resistance of dermatophytes is seldom reported, and the mechanisms associated with resistance are not well known. This study investigated the effect of efflux pump modulators (EPMs) (i.e., haloperidol HAL and promethazine PTZ) and their inhibiting activity on the minimum inhibitory concentrations of itraconazole (ITZ) and fluconazole (FLZ) against selected M. canis strains. M. canis strains with low (≤ 1 μg/ml itraconazole and < 64 μg/ml fluconazole) and high (> 1 μg/ml itraconazole and ≥ 64 μg/ml fluconazole) azole MIC values were tested using Checkerboard microdilution assay. The disk diffusion assay, the minimum fungicidal concentration and the time-kill assay were also performed in order to confirm the results of checkerboard microdilution assay. The MIC values of ITZ and FLZ of M. canis decreased in the presence of subinhibitory concentrations of HAL and PTZ, the latter being more effective with a greater increased susceptibility. Synergism was observed in all strains with high azole MICs (FICI < 0.5) and no synergism in the strains with low azole MICs. A fungicidal activity was observed after 48 h of incubation when ITZ and FLZ were tested in combination with HAL or PTZ. These results suggest that the drug efflux pumps are involved in the defense mechanisms to azole drugs in M. canis strains. The synergism might be related to an increased expression of efflux pump genes, eventually resulting in azole resistance phenomena. Complementary studies on M. canis resistance are advocated in order to investigate the molecular mechanisms of this phenomenon.

journal_name

Mycopathologia

journal_title

Mycopathologia

authors

Aneke CI,Rhimi W,Otranto D,Cafarchia C

doi

10.1007/s11046-019-00419-7

subject

Has Abstract

pub_date

2020-04-01 00:00:00

pages

279-288

issue

2

eissn

0301-486X

issn

1573-0832

pii

10.1007/s11046-019-00419-7

journal_volume

185

pub_type

杂志文章
  • Morphology and phylogeny of Botryosphaeria dothidea causing fruit rot of olives.

    abstract::The taxonomic position of the causal agent of fruit rot of olives was determined from fresh collections of the fungus from central Greece. In culture it formed two types of conidia, namely fusiform, hyaline, aseptate conidia typical of the genus Fusicoccum, and dark-walled, ovoid, ellipsoid or fusiform, 1-2 septate co...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-005-0256-2

    authors: Phillips AJ,Rumbos IC,Alves A,Correia A

    更新日期:2005-04-01 00:00:00

  • Molecular Identification, Genotyping, Phenotyping, and Antifungal Susceptibilities of Medically Important Trichosporon, Apiotrichum, and Cutaneotrichosporon Species.

    abstract::Recently, Trichosporon taxonomy has been reevaluated and new genera of the Trichosporonaceae family have been described. Here, 26 clinical isolates were submitted for identification via sequencing of the intergenic space 1 (IGS1) region, genotyping, and investigation of virulence factors. Antifungal susceptibility was...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-019-00407-x

    authors: do Espírito Santo EPT,Monteiro RC,da Costa ARF,Marques-da-Silva SH

    更新日期:2020-04-01 00:00:00

  • Susceptibility and features of the ultrastructure of Prototheca zopfii following exposure to copper sulphate, silver nitrate and chlorexidine.

    abstract::One of the most important forms of the occurrence of protothecosis is bovine mastitis. Studies on the "in vivo" and "in vitro" susceptibility to antimicrobials have shown that the microorganism is resistant to most of them. Looking for alternative treatments this study aimed to study the susceptibility to copper sulph...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1023/a:1021313118632

    authors: Melville PA,Benites NR,Sinhorini IL,Costa EO

    更新日期:2002-01-01 00:00:00

  • Antifungal susceptibility profile of cryptic species of Aspergillus.

    abstract::The use of molecular tools has led to the description of new cryptic species among different Aspergillus species complexes. Their frequency in the clinical setting has been reported to be between 10 and 15%. The susceptibility to azoles and amphotericin B of many of these species is low, and some of them, such as Aspe...

    journal_title:Mycopathologia

    pub_type: 杂志文章,评审

    doi:10.1007/s11046-014-9775-z

    authors: Alastruey-Izquierdo A,Alcazar-Fuoli L,Cuenca-Estrella M

    更新日期:2014-12-01 00:00:00

  • Involvement of a Ca2+-calmodulin interaction in the yeast-mycelial (Y-M) transition of Candida albicans.

    abstract::A yeast-mycelium (Y-M) transition of Candida albicans (3153A) was induced by 1.5 mM CaCl2.2H2O in defined liquid medium, pH 7, at 25 degrees C. Germ tube formation was detected after approximately 8 h and peaks of maximum germination occurred at approximately 20 h in all experimental treatments. Non-toxic concentratio...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00436783

    authors: Sabie FT,Gadd GM

    更新日期:1989-10-01 00:00:00

  • Breakthrough disseminated Scedosporium prolificans infection in a patient with relapsed leukaemia on prolonged voriconazole followed by posaconazole prophylaxis.

    abstract::A man with acute lymphoblastic leukaemia developed disseminated Scedosporium prolificans infection following chemotherapy for disease relapse while on posaconazole prophylaxis. Scedosporium prolificans infection during posaconazole prophylaxis has not been reported previously. This report is timely as the uptake of po...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-008-9131-2

    authors: Ananda-Rajah MR,Grigg A,Slavin MA

    更新日期:2008-08-01 00:00:00

  • Partial suppression of cell mediated immunity in chromoblastomycosis.

    abstract::The cellular immune response of 8 patients from the Brazilian Amazon region with chromoblastomycosis was analyzed. Primary immunological responses of patients were tested by contact sensitization to 2,4-dinitro-chlorobenzene (DNCB), or rejection of first set skin allografts. 2 of 8 patients were reactive to DNCB after...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00443936

    authors: Fuchs J,Pecher S

    更新日期:1992-08-01 00:00:00

  • A comparative study of Diaporthe/Phomopsis fungi on soybean from two different regions of the world.

    abstract::A comparative study was conducted with soybean material presenting symptoms of the Diaporthe/Phomopsis complex that was collected in two distant geographical regions of the world: Beltsville, MA, USA, and Vojvodina, Yugoslavia. Contrasting with earlier findings, great variability in the disease symptoms was observed, ...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1023/A:1006849020432

    authors: Nevena M,Jelena V,Franić-Mihajlović D

    更新日期:1997-01-01 00:00:00

  • Meningitis Caused by Rhodotorula mucilaginosa in HIV-Infected Patient: A Case Report and Review of the Literature.

    abstract::Rhodotorula species are increasingly being identified as a cause of fungal infection in the central nervous system, especially in patients with compromised immunity. The diagnosis could easily be missed due to low index of suspicion, as cryptococcus meningitis and cerebral toxoplasmosis are more common amongst immunoc...

    journal_title:Mycopathologia

    pub_type: 杂志文章,评审

    doi:10.1007/s11046-015-9879-0

    authors: Mohd Nor F,Tan LH,Na SL,Ng KP

    更新日期:2015-08-01 00:00:00

  • Phospholipase activity in Cryptococcus neoformans.

    abstract::Phospholipases have only been detected in a few fungi and yeasts, in particular in Candida albicans. Secreted phospholipases are considered by some researchers to be a potential factor of virulence and pathogenicity in C. albicans. Twenty-three Cryptococcus neoformans strains were tested in order to observe phospholip...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00438916

    authors: Vidotto V,Sinicco A,Di Fraia D,Cardaropoli S,Aoki S,Ito-Kuwa S

    更新日期:1996-01-01 00:00:00

  • Effects of Photodynamic Therapy on the Growth and Antifungal Susceptibility of Scedosporium and Lomentospora spp.

    abstract::Scedosporium and Lomentospora species are the second most frequent colonizing, allergenic, or invasive fungal pathogens in patients with cystic fibrosis, and are responsible for infections varying from cutaneous and subcutaneous tissue infections caused by traumatic inoculation to severe systemic diseases in immunocom...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-017-0195-8

    authors: Lu Q,Sun Y,Tian D,Xiang S,Gao L

    更新日期:2017-12-01 00:00:00

  • Selective effects of two systemic fungicides on soil fungi.

    abstract::BAS 317 00F was not toxic to the total count of fungi after 2 days but was regularly significantly toxic at the three doses after 5, 20 and 40 days and toxic at the low and the high doses after 80 days. In the agar medium, it was toxic to the counts of total fungi. Aspergillus, A. terreus, Rhizopus oryzae and Mucor ra...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00468085

    authors: Abdel-Fattah HM,Abdel-Kader MI,Hamida S

    更新日期:1982-08-20 00:00:00

  • Systemic candidiasis in mice immunized with Candida albicans ribosomes.

    abstract::In view of our previous findings that vaccination of mice with Candida albicans ribosomes protects them against experimental systemic candidiasis, the aim of this study was to investigate the effect of this vaccination on the course of infection in immunized animals. Since the kidney is the major target in systemic ca...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00437281

    authors: Levy R,Segal E,Barr-Nea L

    更新日期:1985-07-01 00:00:00

  • Prevalence of superficial mycoses in the Aosta Valley region of Italy from 1984 to 1989.

    abstract::This report represents the results of the culture tests for fungi carried out in the Aosta Valley region of Italy from 1984 to 1989. The pathological material consisted of cutaneous scales, hair and nails. It was possible to identify 91.8% of the isolates: out of these, 36.1% were dermatophytes, 21% were Aspergillus s...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00436829

    authors: Dal Tio R,Lunardi M

    更新日期:1991-12-01 00:00:00

  • Challenges in the therapy of chromoblastomycosis.

    abstract::Chromoblastomycosis (CBM) is an implantation mycosis mainly occurring in tropical and subtropical zones worldwide. If not diagnosed at early stages, patients with CBM require long-term therapy with systemic antifungals flanked by various physical treatment regimens. As in other neglected endemic mycoses, comparative c...

    journal_title:Mycopathologia

    pub_type: 杂志文章,评审

    doi:10.1007/s11046-013-9648-x

    authors: Queiroz-Telles F,Santos DW

    更新日期:2013-06-01 00:00:00

  • Effect of roasting on ochratoxin A level in green coffee beans inoculated with Aspergillus ochraceus.

    abstract::The heat stability of ochratoxin A in green coffee beans inoculated with Aspergillus ochraceus was studied. Heat treatment (roasting) at 200 degrees C for 10 or 20 min reduced the levels of ochratoxin A by only 0-12% in the dried whole beans. Almost all of the ochratoxin A was infused into the coffee decoction when th...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00436848

    authors: Tsubouchi H,Yamamoto K,Hisada K,Sakabe Y,Udagawa S

    更新日期:1987-02-01 00:00:00

  • Disseminated trichosporonosis in a neutropenic murine model.

    abstract::Life-threatening disseminated infection with Trichosporon beigelii (trichosporonosis) is a rare mycosis most commonly seen in patients with hematologic malignancies made neutropenic by cytotoxic therapy. This infection is usually resistant to conventional antifungal therapies. Poor correlation between therapeutic outc...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF01103609

    authors: Hospenthal D,Belay T,Lappin P,Rogers A,Kennedy M

    更新日期:1993-05-01 00:00:00

  • Serological detection of antibodies against Paracoccidioides brasiliensis in dogs with leishmaniasis.

    abstract::The aim of this study was to detect antibodies against Paracoccidioides brasiliensis in dogs seropositive and seronegative for leishmaniasis. Sera from 836 dogs (449 positive and 387 negative to leishmaniasis) were analysed by ELISA and the immunodiffusion test using gp43 and exoantigen, respectively. The analysis of ...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-006-0046-5

    authors: Silveira LH,Domingos IH,Kouchi K,Itano EN,Silva EA,Landgraf VO,Werneck SM,Camargo ZP,Ono MA

    更新日期:2006-11-01 00:00:00

  • The First Case of Total Dystrophic Onychomycosis Caused by Aspergillus clavatus Resistant to Antifungal Drugs.

    abstract::Onychomycosis is a common fungal infection of nails which is mainly caused by dermatophyte species and less often by yeasts and non-dermatophyte molds. We present a case of onychomycosis due to Aspergillus clavatus for the first time worldwide. The patient was an immunocompetent 32-year-old woman who identified with P...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-015-9954-6

    authors: Falahati M,Ghojoghi A,Abastabar M,Ghasemi Z,Farahyar S,Roudbary M,Hedayati MT,Armaki MT,Hoseinnejad A

    更新日期:2016-04-01 00:00:00

  • Occurrence of mycotoxins in cereals and animal feedstuffs in Natal, South Africa 1994.

    abstract::During the year of 1994, 417 samples of agricultural commodities, comprising: maize, compound animal feeds, oil seeds, soya bean, fish meal and forage were examined for fungi and over 20 mycotoxins using a multi-screen augmented with individual assays. Trichothecenes had the highest incidence of over 19% in all sample...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF01103901

    authors: Dutton MF,Kinsey A

    更新日期:1995-07-01 00:00:00

  • Pseudomonas aeruginosa inhibits the growth of Cryptococcus species.

    abstract::Pseudomonas aeruginosa is a ubiquitous and opportunistic bacterium that inhibits the growth of different microorganisms, including Gram-positive bacteria and fungi such as Candida spp. and Aspergillus fumigatus. In this study, we investigated the interaction between P. aeruginosa and Cryptococcus spp. We found that P....

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-011-9494-7

    authors: Rella A,Yang MW,Gruber J,Montagna MT,Luberto C,Zhang YM,Del Poeta M

    更新日期:2012-06-01 00:00:00

  • Action of chlorhexidine on budding Candida albicans: scanning and transmission electron microscopic study.

    abstract::Chlorhexidine is widely used as a bacterial drug whose method of action has been well described in bacteria. Its fungicidal properties have been proved. We show here the effects of a sublethal dose of a preparation of digluconate of chlorhexidine on budding Candida albicans. A fungistatic action is revealed by a decre...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00769565

    authors: Bobichon H,Bouchet P

    更新日期:1987-10-01 00:00:00

  • Determination of the minimal inhibitory concentration of amphotericin B and miconazole for 21 strains of Aspergillus.

    abstract::The susceptibility of 21 strains of Aspergillus (11 of A. fumigatus, 8 of A, niger, and 2 of A. flavus) isolated from human pathologic specimens to Amphotericin B and Miconazole has been comparatively studied. Determination of the minimal inhibitory concentration of both drugs in a liquid medium showed a noticeably va...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00576365

    authors: Martinez J,Torres JM,Arteaga F,Foz-Tena A

    更新日期:1978-11-10 00:00:00

  • Antifungal activities of four fatty acids against plant pathogenic fungi.

    abstract::The effect of the fatty acids linolenic acid, linoleic acid, erucic acid and oleic acid on the growth of the plant pathogenic fungi Rhizoctonia solani, Pythium ultimum, Pyrenophora avenae and Crinipellis perniciosa were examined in in vitro studies. Linolenic and linoleic acids exhibited activity against all of the fu...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1023/b:myco.0000012222.68156.2c

    authors: Walters D,Raynor L,Mitchell A,Walker R,Walker K

    更新日期:2004-01-01 00:00:00

  • Identification of Aspergillus section Flavi in maize in northeastern China.

    abstract::Species of Aspergillus section Flavi were isolated and identified in maize samples collected from a wide geographical region in northeastern China. Aspergillus flavus was identified to be the primary species in the population (99%) becoming a potential source of aflatoxin contamination in the region. Isolated A. flavu...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-007-9029-4

    authors: Gao J,Liu Z,Yu J

    更新日期:2007-08-01 00:00:00

  • Ecological niche of Cryptococcus neoformans var. grubii and Cryptococcus gattii in decaying wood of trunk hollows of living trees in Jabalpur City of Central India.

    abstract::Cryptococcus neoformans var. grubii and C. gattii were repeatedly isolated from decaying wood of trunk hollows in living trees growing in Jabalpur City in Central India. The isolation of C. gattii has been reported from decayed wood inside trunk hollow of Tamarindus indica (15.6%), Mangifera indica (2.2%), Pithecolobi...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-007-9039-2

    authors: Grover N,Nawange SR,Naidu J,Singh SM,Sharma A

    更新日期:2007-10-01 00:00:00

  • In vitro sensitivity of Verticillium fungicola to selected fungicides.

    abstract::Twenty isolates of Verticillium fungicola var. fungicola collected from diseased fruit-bodies of Agaricus bisporus from prochloraz-treated crops, were exposed to a range of concentrations of six chemicals (benomyl, chlorothalonil, formaldehyde, iprodione, prochloraz-Mn-complex and prochloraz + carbendazim) in vitro. E...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00438918

    authors: Gea FJ,Tello JC,Honrubia M

    更新日期:1996-12-01 00:00:00

  • Effect of phytate on aflatoxin formation by Aspergillus parasiticus and Aspergillus flavus in synthetic media.

    abstract::The effect of phytate on the production of aflatoxins by Aspergillus parasiticus and Aspergillus flavus grown on synthetic media was examined. In the absence of pH control (initial pH 4.5-6.5) for A. parasiticus, phytate (14.3 mM) caused a six-fold decrease in aflatoxins in the medium and a ten-fold decrease in those ...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/BF00436636

    authors: Ehrlich K,Ciegler A

    更新日期:1984-08-30 00:00:00

  • Anti-Candida Activity of New Azole Derivatives Alone and in Combination with Fluconazole.

    abstract::The rate of Candida spp. infection is increasing, and resistance to azole antifungals is becoming increasingly common. Therefore, there is a need for discovery of new antifungal agents and for development of new modes of treatment using existing agents. In this in vitro study, the antifungal activity of two new imidaz...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-015-9899-9

    authors: Shi C,Liu C,Liu J,Wang Y,Li J,Xiang M

    更新日期:2015-10-01 00:00:00

  • Detection of (1, 3)-β-D-glucan in bronchoalveolar lavage and serum samples collected from immunocompromised hosts.

    abstract::The incidence of invasive fungal infections (IFI) has increased in recent years, especially among immunocompromised hosts (ICH). In 2003, the Fungitell(®) assay received FDA clearance for the presumptive diagnosis of IFI using serum and detects (1-3)-β-D-glucan, which is a major cell wall component of certain fungi (e...

    journal_title:Mycopathologia

    pub_type: 杂志文章

    doi:10.1007/s11046-012-9579-y

    authors: Theel ES,Jespersen DJ,Iqbal S,Bestrom JE,Rollins LO,Misner LJ,Markley BJ,Mandrekar J,Baddour LM,Limper AH,Wengenack NL,Binnicker MJ

    更新日期:2013-02-01 00:00:00