Abstract：
:In cytochrome c oxidase (CytcO) reduction of O2 to water is linked to uptake of eight protons from the negative side of the membrane: four are substrate protons used to form water and four are pumped across the membrane. In bacterial oxidases, the substrate protons are taken up through the K and the D proton pathways, while the pumped protons are transferred through the D pathway. On the basis of studies with CytcO isolated from bovine heart mitochondria, it was suggested that in mitochondrial CytcOs the pumped protons are transferred though a third proton pathway, the H pathway, rather than through the D pathway. Here, we studied these reactions in S. cerevisiae CytcO, which serves as a model of the mammalian counterpart. We analyzed the effect of mutations in the D (Asn99Asp and Ile67Asn) and H pathways (Ser382Ala and Ser458Ala) and investigated the kinetics of electron and proton transfer during the reaction of the reduced CytcO with O2. No effects were observed with the H pathway variants while in the D pathway variants the functional effects were similar to those observed with the R. sphaeroides CytcO. The data indicate that the S. cerevisiae CytcO uses the D pathway for proton uptake and presumably also for proton pumping.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Björck ML,Vilhjálmsdóttir J,Hartley AM,Meunier B,Näsvik Öjemyr L,Maréchal A,Brzezinski Pdoi
10.1038/s41598-019-56648-9subject
Has Abstractpub_date
2019-12-27 00:00:00pages
20207issue
1issn
2045-2322pii
10.1038/s41598-019-56648-9journal_volume
9pub_type
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