Identification of a key gene module associated with glucocorticoid- induced derangement in bone mineral density in patients with asthma.

Abstract:

:Derangement in bone mineral density (BMD) caused by glucocorticoid is well-known. The present study aimed to find key biological pathways associated with low BMD after glucocorticoid treatment in asthmatics using gene expression profiles of peripheral blood cells. We utilized immortalized B cells (IBCs) from 32 childhood asthmatics after multiple oral glucocorticoid bursts and peripheral blood mononuclear cells (PBMCs) from 17 adult asthmatics after a long-term use of oral glucocorticoid. We searched co-expressed gene modules significantly related with the BMD Z score in childhood asthmatics and tested if these gene modules were preserved and significantly associated with the BMD Z score in adult asthmatics as well. We identified a gene module composed of 199 genes significantly associated with low BMD in both childhood and adult asthmatics. The structure of this module was preserved across gene expression profiles. We found that the cellular metabolic pathway was significantly enriched in this module. Among 18 hub genes in this module, we postulated that 2 genes, CREBBP and EP300, contributed to low BMD following a literature review. A novel biologic pathway identified in this study highlighted a gene module and several genes as playing possible roles in the pathogenesis of glucocorticoid- induced derangement in BMD.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Lee SY,Won HK,Kim BK,Kim SH,Chang YS,Cho SH,Kelly HW,Tantisira KG,Park HW

doi

10.1038/s41598-019-56656-9

subject

Has Abstract

pub_date

2019-12-27 00:00:00

pages

20133

issue

1

issn

2045-2322

pii

10.1038/s41598-019-56656-9

journal_volume

9

pub_type

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