Abstract:
:There is a growing interest concerning the relevance of salivary cortisone levels in stress-related research. However, studies investigating morning patterns and day-to-day variability of cortisone versus cortisol levels are lacking. Cortisol and cortisone analysis by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) has been widely used for routine laboratory measurements in the last years. The aim of this study was to develop an ultra-performance LC-MS/MS method for the simultaneous quantification of salivary cortisol and cortisone levels for assessing the temporal variability of these hormones. Saliva samples were collected from 18 healthy volunteers at 0, 15, and 30 min after awakening on each day for 1 week and analysed with the newly developed method. We used a novel atmospheric pressure ionization source, which resulted in high sensitivity and specificity for both cortisol and cortisone as well as higher peak values and signal-to-noise ratio as compared with the electrospray ionization source. Cortisone showed similar morning patterns as cortisol: a 25% and 49% increase in levels at 15 and 30 min after awakening, respectively. Most cortisone indices showed somewhat lower day-to-day variability and were less affected by state-related covariates. We recommend further exploration of the potential of salivary cortisone as a biomarker in stress-related research.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Bakusic J,De Nys S,Creta M,Godderis L,Duca RCdoi
10.1038/s41598-019-55571-3subject
Has Abstractpub_date
2019-12-17 00:00:00pages
19313issue
1issn
2045-2322pii
10.1038/s41598-019-55571-3journal_volume
9pub_type
杂志文章abstract::A light reflection device combined with visible light spectrophotometer was used to measure the reflectance-wavelength curves of colored stainless steel after coloring, hardening and sealing. After these processes, the coordinate in CIE 1931 color coordinate system and brightness of samples were confirmed by chromatom...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70359-6
更新日期:2020-08-11 00:00:00
abstract::Parent selection is the core of hybrid breeding. The breeding strategy involving the parental identification of superior open-pollinated progeny of Populous tomentosa germplasm resources can significantly improve the efficiency of parental matching. However, due to some factors such as loose powdering time and pollen ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74941-w
更新日期:2020-10-29 00:00:00
abstract::For a number of years nanomaterials have been continuously devised and comprehensively investigated because of the growing demand for them and their multifarious applications, especially in medicine. This paper reports on the properties of SrF2 nanoparticles (NPs) for applications in biomedicine, showing effective way...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45025-1
更新日期:2019-06-17 00:00:00
abstract::Advances in Big Data make it possible to make short-term forecasts for market trends from previously unexplored sources. Trading strategies were recently developed by exploiting a link between the online search activity of certain terms semantically related to finance and market movements. Here we build on these earli...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50131-1
更新日期:2019-09-18 00:00:00
abstract::Modes attenuation of the tube lattice fiber (TLF) is characterized by D/λ, where D is the core diameter and λ is the wavelength. Hence, the TLF is structured with a large core to ensure a low attenuation loss. A small core, on the other hand, facilitates the gas-filled TLF applications, but at the expense of the incre...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01839-5
更新日期:2017-05-09 00:00:00
abstract::Genome-wide studies on high-altitude adaptation have received increased attention as a classical case of organismal evolution under extreme environment. However, the current genetic understanding of high-altitude adaptation emanated mainly from autosomal analyses. Only a few earlier genomic studies paid attention to t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35004
更新日期:2016-10-07 00:00:00
abstract::Previous studies have suggested that microRNA-107 (miR-107) regulates cell migration in tumor and promotes Hypoxia Inducible Factor 1α (HIF1α) regulated angiogenesis under hypoxia. We found that miR-107 was strongly expressed in ischemic boundary zone (IBZ) after permanent middle cerebral artery occlusion (pMCAO) in r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13316
更新日期:2015-08-21 00:00:00
abstract::The Rho-family small GTPase Cdc42 localizes at plasma membrane and Golgi complex and aside from protrusion and migration operates in vesicle trafficking, endo- and exocytosis as well as establishment and/or maintenance of cell polarity. The formin family members FMNL2 and -3 are actin assembly factors established to r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09952-1
更新日期:2017-08-29 00:00:00
abstract::Accurate repair of DNA damage is crucial to ensure genome stability and cell survival of all organisms. Bile functions as a defensive barrier against intestinal colonization by pathogenic microbes. Campylobacter jejuni, a leading bacterial cause of foodborne illness, possess strategies to mitigate the toxic components...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14646-9
更新日期:2017-10-31 00:00:00
abstract::Potato (Solanum tuberosum) is an important staple crop worldwide, it has been cultivated in the Andean Altiplano under low-input farming practices at high altitudes and under harsh environment for centuries. We analyzed secondary metabolite (SM) gene diversity encoded in the potato rhizosphere microbiome during plant ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02314-x
更新日期:2017-05-24 00:00:00
abstract::The knee joint center of rotation is altered in the absence of the anterior cruciate ligament, which leads to substantially higher variance in kinematic patterns. To overcome this, total knee arthroplasty (TKA) designs with a high congruency in the lateral compartment have been proposed. The purpose of this study was ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45694-y
更新日期:2019-06-24 00:00:00
abstract::From biomarkers to drug carriers, Extracellular Vesicles (EVs) are being used successfully in numerous applications. However, while the subject has been steadily rising in popularity, current methods of isolating EVs are lagging behind, incapable of isolating EVs at a high enough quantity or quality while also requiri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55477-0
更新日期:2019-12-16 00:00:00
abstract::To meet the demand for detection of foreign genes in genetically modified (GM) sugarcane necessary for regulation of gene technology, an efficient method with high specificity and rapidity was developed for the cry1Ac gene, based on loop-mediated isothermal amplification (LAMP). A set of four primers was designed usin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04912
更新日期:2014-05-09 00:00:00
abstract::One of the key motivations in producing 3D structures has always been the realization of metamaterials with effective constituent properties that can be tuned in all propagation directions at various frequencies. Here, we report the investigation of spatially oriented "Nanograter" structures with orientation-dependent...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28764
更新日期:2016-06-30 00:00:00
abstract::We have designed a versatile and sensitive liquid chromatographic (LC) system, featuring a monolithic trap column and a very narrow (10 μm ID) fused silica open tubular liquid chromatography (OTLC) separation column functionalized with C18-groups, for separating a wide range of molecules (from small metabolites to int...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37507
更新日期:2016-11-29 00:00:00
abstract::Most pancreatic cancer (PC) patients manifest multiple liver metastases at the time of diagnosis. Activated platelets play a key role in tumor growth and tumor metastases. Mean platelet volume (MPV) is a platelet index and is altered in patients with malignancies. This study aimed to evaluate whether MPV can effective...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24539-0
更新日期:2018-04-16 00:00:00
abstract::Oxygen-induced retinopathy (OIR) upregulates Müller cell vascular endothelial growth factor A (VEGFA) that causes intravitreal neovascularization similar to severe retinopathy of prematurity (ROP). Safety concerns exist with anti-VEGF treatment for ROP. We evaluated long-term knockdown of Müller cell-VEGFA with short-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20278-4
更新日期:2018-01-31 00:00:00
abstract::Vaginal microbiota is an important early source of bacterial colonization for newborns. However, only a few small studies have investigated the composition of vaginal microbiota during labor. In this work, we analyzed vaginal swabs collected at 36 weeks gestation and at the onset of labor from 256 women participating ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17972-0
更新日期:2017-12-14 00:00:00
abstract::Liquid pumping can occur along the outer surface of an electrode under a DC electric field. For biological applications, a better understanding of the ionic solution pumping mechanism is required. Here, we fabricated CNT wire electrodes (CWEs) and tungsten wire electrodes (TWEs) of various diameters to assess an ionic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11799
更新日期:2015-07-02 00:00:00
abstract::Wet-chemistry methods have crucial advantages for the synthesis of nanostructures, including simple, low-cost, large-area, and low-temperature deposition on almost arbitrary substrates. Nevertheless, the rational design of improved wet-chemistry procedures is extremely difficult because, in practice, only post-synthes...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06285
更新日期:2014-09-05 00:00:00
abstract::The current standard of care for head and neck cancer includes surgical resection of the tumor followed by targeted head and neck radiation. This radiotherapy results in a multitude of negative side effects in adjacent normal tissues. Autophagy is a cellular mechanism that could be targeted to ameliorate these side ef...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05206
更新日期:2014-06-06 00:00:00
abstract::HIV subtypes or clades differentially induce HIV-associated neurocognitive disorders (HAND) and substance abuse is known to accelerate HIV disease progression. The HIV-1 envelope protein gp120 plays a major role in binding and budding in the central nervous system (CNS) and impacts dopaminergic functions. However, the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11130
更新日期:2015-06-09 00:00:00
abstract::Determining oil migration distances from source rocks to reservoirs can greatly help in the search for new petroleum accumulations. Concentrations and ratios of polar organic compounds are known to change due to preferential sorption of these compounds in migrating oils onto immobile mineral surfaces. However, these c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02487
更新日期:2013-01-01 00:00:00
abstract::Activation of the inflammasome is involved in the progression of retinal degenerative diseases, in particular, in the pathogenesis of Age-Related Macular Degeneration (AMD), with NLRP3 activation the focus of many investigations. In this study, we used genetic and pharmacological approaches to explore the role of the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58849-z
更新日期:2020-02-10 00:00:00
abstract::Fundamental cell signaling mechanisms that regulate dynamic remodeling of the extracellular matrix (ECM) in mechanically loaded tissues are not yet clearly understood. Trabecular meshwork (TM) tissue in the eye is under constant mechanical stress and continuous remodeling of ECM is crucial to maintain normal aqueous h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00845-x
更新日期:2017-04-11 00:00:00
abstract::Little information is available on chemical forms of heavy metals on integrate plant roots. KNO3 (1 M), 0.05M EDTA at pH6 and 0.01 M HCl were used sequentially to extract the exchangeable, complexed and precipitated forms of Cu(II) and Cd(II) from soybean roots and then to investigate chemical form distribution of Cu(...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36373
更新日期:2016-11-02 00:00:00
abstract::Effective application of whole-cell devices in synthetic biology and biocatalysis will always require consideration of the uptake of molecules of interest into the cell. Here we demonstrate that the AlkL protein from Pseudomonas putida GPo1 is an alkane import protein capable of industrially relevant rates of uptake o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05844
更新日期:2014-07-28 00:00:00
abstract::Diffusion magnetic resonance imaging (dMRI) proved promising in patients with non-myelopathic degenerative cervical cord compression (NMDCCC), i.e., without clinically manifested myelopathy. Aim of the study is to present a fast multi-shell HARDI-ZOOMit dMRI protocol and validate its usability to detect microstructura...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70297-3
更新日期:2020-10-16 00:00:00
abstract::The East Asian Summer Monsoon driven by temperature and moisture gradients between the Asian continent and the Pacific Ocean, leads to approximately 50% of the annual rainfall in the region across 20-40°N. Due to its increasing scientific and social importance, there have been several previous studies on identificatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22266-0
更新日期:2018-03-16 00:00:00
abstract::The fungal microbiota is an important component of the human gut microbiome and may be linked to gastrointestinal disease. In this study, the fungal microbiota of biopsy samples from adenomas and adjacent tissues was characterized by deep sequencing. Ascomycota, Glomeromycota and Basidiomycota were identified as the d...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/srep07980
更新日期:2015-01-23 00:00:00