Abstract:
:From biomarkers to drug carriers, Extracellular Vesicles (EVs) are being used successfully in numerous applications. However, while the subject has been steadily rising in popularity, current methods of isolating EVs are lagging behind, incapable of isolating EVs at a high enough quantity or quality while also requiring expensive, specialized equipment. The "isolation problem" is one of the major obstacles in the field of EV research - and even more so for their potential, widespread use for clinical diagnosis and therapeutic applications. Aqueous Two-Phase Systems (ATPS) has been reported previously as a promising method for isolating EVs quickly and efficiently, and with little contaminants - however, this method has not seen widespread use. In this study, an ATPS-based isolation protocol is used to isolate small EVs from plant, cell culture, and parasite culture sources. Isolated EVs were characterized in surface markers, size, and morphological manner. Additionally, the capacity of ATPS-based EV isolation in removing different contaminants was shown by measuring protein, fatty acid, acid, and phenol red levels of the final isolate. In conclusion, we have shown that EVs originating from different biological sources can be isolated successfully in a cost-effective and user-friendly manner with the use of aqueous two-phase systems.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kırbaş OK,Bozkurt BT,Asutay AB,Mat B,Ozdemir B,Öztürkoğlu D,Ölmez H,İşlek Z,Şahin F,Taşlı PNdoi
10.1038/s41598-019-55477-0subject
Has Abstractpub_date
2019-12-16 00:00:00pages
19159issue
1issn
2045-2322pii
10.1038/s41598-019-55477-0journal_volume
9pub_type
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