[Microinjection of spermatozoa into the oocyte. An experimental model for assisted conjugation in oligo-asthenozoospermia as a cause of sterility].

Abstract:

:This study investigates the possibilities to treat oligo-asthenozoospermia, a cause of sterility, by assisted conjugation. After completing acrosome reaction, normal motile spermatozoa are microinjected into mouse oocytes. After 2 hours of incubation time in medium with lactate and albumins, spermatozoa are put into dibutyryl 3',5'-guanosine monophosphate (dbcGMP) and imidazole for the completion of an acrosome reaction. One motile spermatozoon each was injected microsurgically into the perivitelline space of an oocyte. The survival rate of the oocytes after injection was around 85%. The oocytes with two pronuclei and a second polar body were considered as fertilized. Of those injected oocytes 21.5% were fertilized with acrosome reacted spermatozoa, whereas only 5.6% were fertilized with spermatozoa without dbcGMP treatment. Thus, motile and acrosome reacted spermatozoa essentially fertilize oocytes at microinjection into the perivitelline space very well. This technique may become a hopeful procedure to treat human oligo-asthenozoospermia in an IVF/ET-programme.

authors

Mettler L,Yamada K,Kuranty A

doi

10.1055/s-2008-1026552

subject

Has Abstract

pub_date

1988-09-01 00:00:00

pages

625-9

issue

9

eissn

0016-5751

issn

1438-8804

journal_volume

48

pub_type

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