MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase.

Abstract:

PURPOSE:Adipogenic differentiation of adipose tissue-derived mesenchymal stem cells (AMSCs) is critical to many disease-related disorders, such as obesity and diabetes. Studies have demonstrated that miRNA-138 (miR-138) is closely involved in adipogenesis. However, the mechanisms affected by miR-138 remain unclear. This work aimed to investigate interactions between miR-138 and lipoprotein lipase (LPL), a key lipogenic enzyme, in AMSCs. MATERIALS AND METHODS:Human AMSCs (hAMSCs) isolated from human abdomen tissue were subjected to adipogenic differentiation medium. Quantitative real-time polymerase chain reaction and Western blot assay were applied to measure the expressions of miR-138, LPL, and the two adipogenic transcription factors cytidine-cytidine-adenosine-adenosine-thymidine enhancer binding protein alpha (C/EBPα) and peroxisome proliferator-activated receptor gamma (PPARγ). The relationship between miR-138 and LPL was predicted utilizing the miRTarBase database and validated by dual luciferase reporter assay. RESULTS:Showing increases in C/EBPα and PPARγ expression levels, hAMSCs were induced into adipogenic differentiation. During adipogenesis of hAMSCs, miR-138 expression was significantly downregulated. Overexpression of miR-138 by transfection inhibited hAMSCs adipogenic differentiation in vitro. Mechanically, LPL was a target of miR-138. LPL expression was upregulated during adipogenesis of hAMSCs, and this upregulation was reversed by miR-138 overexpression. Functionally, silencing of LPL by transfection exerted similar inhibition of the expressions of C/EBPα and PPARγ. Meanwhile, LPL ectopic expression was able to partly abolish the suppressive effect of miR-138 overexpression on adipogenic differentiation of hAMSCs. CONCLUSION:Upregulation of miR-138 inhibits adipogenic differentiation of hAMSCs by directly downregulating LPL.

journal_name

Yonsei Med J

journal_title

Yonsei medical journal

authors

Wang Y,Lin L,Huang Y,Sun J,Wang X,Wang P

doi

10.3349/ymj.2019.60.12.1187

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

1187-1194

issue

12

eissn

0513-5796

issn

1976-2437

pii

60.1187

journal_volume

60

pub_type

杂志文章
  • Posterior lumbar interbody fusion via a unilateral approach.

    abstract::This study sought to determine the outcomes of posterior lumbar interbody fusion (PLIF), via a unilateral approach, in selected patients who presented with unilateral leg pain and segmental instability of the lumbar spine. Patients with a single level of a herniated disc disease in the lumbar spine, unilateral leg pai...

    journal_title:Yonsei medical journal

    pub_type: 临床试验,杂志文章

    doi:10.3349/ymj.2006.47.3.319

    authors: Shin HC,Yi S,Kim KN,Kim SH,Yoon DH

    更新日期:2006-06-30 00:00:00

  • Chlorophyll derivatives (CpD) extracted from silk worm excreta are specifically cytotoxic to tumor cells in vitro.

    abstract::Among chlorophyll derivatives (CpD-A, -B, -C, and -D) extracted from silk worm (Bombyx mori) excretas, CpD-A was extensively studied to clarify its role as a "photosensitizer" for photodynamic therapy (PDT) of tumors in vitro. It was found that CpD-A was photoreactive both in itself and also in its cell bound forms. T...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.1990.31.3.225

    authors: Lee WY,Park JH,Kim BS,Han MJ,Hahn BS

    更新日期:1990-09-01 00:00:00

  • Mini-Open Anterior Retroperitoneal Lumbar Interbody Fusion: Oblique Lateral Interbody Fusion for Lumbar Spinal Degeneration Disease.

    abstract:PURPOSE:Surgery for lumbar spinal degeneration disease is widely performed. While posterior decompression and fusion are popular, anterior lumbar interbody fusion (ALIF) is also used for treatment. Extreme lateral interbody fusion (XLIF) is commonly used for noninvasive ALIF; however, several complications, such as spi...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2015.56.4.1051

    authors: Ohtori S,Orita S,Yamauchi K,Eguchi Y,Ochiai N,Kishida S,Kuniyoshi K,Aoki Y,Nakamura J,Ishikawa T,Miyagi M,Kamoda H,Suzuki M,Kubota G,Sakuma Y,Oikawa Y,Inage K,Sainoh T,Sato J,Fujimoto K,Shiga Y,Abe K,Toyone T

    更新日期:2015-07-01 00:00:00

  • Manchester Operation: An Effective Treatment for Uterine Prolapse Caused by True Cervical Elongation.

    abstract:PURPOSE:Descent of the uterus is a major etiology of uterine prolapse. However, true cervical elongation can cause uterine prolapse without uterine descent. The aim of study was to investigate the clinical outcomes of Manchester operation in patients with uterine prolapse caused by "true cervical elongation," compared ...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2019.60.11.1074

    authors: Park YJ,Kong MK,Lee J,Kim EH,Bai SW

    更新日期:2019-11-01 00:00:00

  • Evaluation of CSF motion in syringomyelia with spatial modulation of magnetization (SPAMM).

    abstract::CSF flow dynamics has been known to have a key role the pathogenesis of syringomyelia. Our purpose was to evaluate the CSF flow pattern in syringomyelia with spatial modulation of magnetization (SPAMM) that can depict directional flow, and to determine the relationship between flow dynamics and clinical outcome after ...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2002.43.1.37

    authors: Lee SK,Chung TS,Kim YS

    更新日期:2002-02-01 00:00:00

  • Anterior transposition of inferior oblique muscle for treatment of unilateral superior oblique muscle palsy with inferior oblique muscle overaction.

    abstract::Although many weakening procedures for the inferior oblique muscle have been advocated, there is some controversy as to the most beneficial procedure for weakening overacting inferior oblique muscles. This study was undertaken to determine if unilateral anterior transposition of the inferior oblique muscle alone could...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2004.45.4.609

    authors: Chang YH,Ma KT,Lee JB,Han SH

    更新日期:2004-08-31 00:00:00

  • The Effects of Short-Term and Very Short-Term Particulate Matter Exposure on Asthma-Related Hospital Visits: National Health Insurance Data.

    abstract:PURPOSE:The purpose of this study was to investigate the effects of short-term and very short-term exposure to particulate matter (PM) exceeding the daily average environmental standards for Korea (≤100 μg/m³ for PM10 and ≤50 μg/m³ for PM2.5) on on asthma-related hospital visits. MATERIALS AND METHODS:This was a popul...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2019.60.10.952

    authors: Song DJ,Choi SH,Song WJ,Park KH,Jee YK,Cho SH,Lim DH

    更新日期:2019-10-01 00:00:00

  • Solitary rectal ulcer syndrome.

    abstract::We report three cases of solitary rectal ulcer syndrome (SRUS). The diagnosis was established according to histopathologic criteria. But, the initial clinical diagnosis was carcinoma, non-specific ulcer and localized proctitis respectively. SRUS is considered as one of functional disorder in pelvic floor which might g...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.1994.35.2.223

    authors: Park HJ,Kim WH,Woo JS,Han KH,Lee SI,Park IS,Han JY,Park CI

    更新日期:1994-06-01 00:00:00

  • P53 overexpression in gastric adenocarcinoma with Helicobacter pylori infection.

    abstract::Gastric carcinogenesis has been studied in various aspects. Helicobacter pylori (Hp) infection and mutation of the p53 tumor suppressor gene have recently been argued to be important factors of gastric carcinogenesis. There have been many studies to determine the precise mechanism of how Hp is related to gastric cance...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.1997.38.2.117

    authors: Chang KH,Kwon JW,Kim BS,Hwang JH,Lee SK,Lee YC,Park HJ,Park IS,Kim HK

    更新日期:1997-04-01 00:00:00

  • Disturbed eating attitudes and behaviors in South Korean boys and girls: a school-based cross-sectional study.

    abstract:PURPOSE:This study was designed to assess the prevalence and correlates of disturbed eating attitudes and behaviors in South Korean students. MATERIALS AND METHODS:In a cross-sectional survey, 2,226 fourth and seventh grade students filled out questionnaires on eating attitudes and behaviors (Eating Attitude Test -26,...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2010.51.3.302

    authors: Yang SJ,Kim JM,Yoon JS

    更新日期:2010-05-01 00:00:00

  • Retrospective assessment of the validity of robotic surgery in comparison to open surgery for pediatric choledochal cyst.

    abstract:PURPOSE:We evaluated the validity of robotic surgery (RS) for pediatric choledochal cyst (CC) in comparison to open surgery (OS). MATERIALS AND METHODS:From January 2009 to April 2013, clinical data from 79 consecutive pediatric patients with CC, who underwent RS (n=36) or OS (n=43) performed by a single pediatric sur...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2015.56.3.737

    authors: Kim NY,Chang EY,Hong YJ,Park S,Kim HY,Bai SJ,Han SJ

    更新日期:2015-05-01 00:00:00

  • The effect of sodium cromoglycate on the induction of experimental IgA nephropathy.

    abstract::Mesangial IgA nephropathy was experimentally induced in ddY mice by oral and parenteral administration of the poliomyelitis vaccine (POLIO), and we then tried to investigate if IgA deposition could be prevented by the concurrent use of sodium cromoglycate (SCG), which is known to inhibit the local mucosal immune react...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.1990.31.1.33

    authors: Jin SY,Choi IJ

    更新日期:1990-03-01 00:00:00

  • Label Adherence for Non-Vitamin K Antagonist Oral Anticoagulants in a Prospective Cohort of Asian Patients with Atrial Fibrillation.

    abstract:PURPOSE:Label adherence for non-vitamin K antagonist oral anticoagulants (NOACs) has not been well evaluated in Asian patients with non-valvular atrial fibrillation (AF). The present study aimed to assess label adherence for NOACs in a Korean AF population and to determine risk factors of off-label prescriptions of NOA...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章,多中心研究

    doi:10.3349/ymj.2019.60.3.277

    authors: Lee SR,Lee YS,Park JS,Cha MJ,Kim TH,Park J,Park JK,Lee JM,Kang KW,Shim J,Uhm JS,Kim J,Kim C,Kim JB,Park HW,Joung B,Choi EK

    更新日期:2019-03-01 00:00:00

  • Hemodynamic, Autonomic, and Vascular Function Changes after Sleep Deprivation for 24, 28, and 32 Hours in Healthy Men.

    abstract::This study aimed to analyze the impact of sleep deprivation (SD) on cardiac, hemodynamic, and endothelial parameters and to determine whether these are sustained with increased periods of SD. The study included 60 healthy men (mean: age 31.2±6.3 years; body mass index 24.6±2.6 kg/m²). Hemodynamic parameters, parameter...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2018.59.9.1138

    authors: Słomko J,Zawadka-Kunikowska M,Kozakiewicz M,Klawe JJ,Tafil-Klawe M,Newton JL,Zalewski P

    更新日期:2018-11-01 00:00:00

  • Modified Peritoneal Dialysis Catheter Insertion: Comparison with a Conventional Method.

    abstract:PURPOSE:The conventional trocar and cannula method in peritoneal dialysis (PD) catheter insertion has its limitation in clinical setting. The aim of this study was to compare a modified method for percutaneous PD catheter insertion with the conventional method, and demonstrate advantages of the modified method. MATERI...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2015.56.4.981

    authors: Lee YK,Yang PS,Park KS,Choi KH,Kim BS

    更新日期:2015-07-01 00:00:00

  • mTOR Signaling Combined with Cancer Stem Cell Markers as a Survival Predictor in Stage II Colorectal Cancer.

    abstract:PURPOSE:Wnt and mammalian target of rapamycin (mTOR) are major molecular signaling pathways associated with the development and progression of tumor, as well as the maintenance and proliferation of cancer stem cells (CSCs), in colorectal cancer (CRC). Identifying patients at risk of poor prognosis is important to deter...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2020.61.7.572

    authors: Chang JY,Kim JH,Kang J,Park Y,Park SJ,Cheon JH,Kim WH,Kim H,Park JJ,Kim TI

    更新日期:2020-07-01 00:00:00

  • Treatment of osteofibrous dysplasia and associated lesions.

    abstract:PURPOSE:To report long term treatment outcomes of osteofibrous dysplasia and association with adamantinoma. PATIENTS AND METHODS:From January 1984 to July 2001, 14 patients with osteofibrous dysplasia were followed for an average of 108 months (78 to 260 months). Our patient group consisted of 6 men and 8 women, with ...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2007.48.3.502

    authors: Hahn SB,Kim SH,Cho NH,Choi CJ,Kim BS,Kang HJ

    更新日期:2007-06-30 00:00:00

  • Effects of epithelium on the mechanism of mediator release from guinea pig tracheal tissues sensitized by IgG1 versus IgE antibody.

    abstract::In the present work, we have examined the effect of PAF, removal of epithelium, the mechanism of desensitization, and the substances that increases the level of intracellular c-AMP on the differences of mediator release from superfused tracheal strips after passive sensitization with IgG1 versus IgE Ab. In the passive...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.1995.36.2.161

    authors: Ro JY,Kim KH

    更新日期:1995-05-01 00:00:00

  • Comparison of Outcomes after Device Closure and Medication Alone in Patients with Patent Foramen Ovale and Cryptogenic Stroke in Korean Population.

    abstract:PURPOSE:To compare the effectiveness of device closure and medical therapy in prevention of recurrent embolic event in the Korean population with cryptogenic stroke and patent foramen ovale (PFO). MATERIALS AND METHODS:Consecutive 164 patients (men: 126 patients, mean age: 48.1 years, closure group: 72 patients, medic...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2016.57.3.621

    authors: Moon J,Kang WC,Kim S,Oh PC,Park YM,Chung WJ,Choi DY,Lee JY,Lee YB,Hwang HY,Ahn T

    更新日期:2016-05-01 00:00:00

  • Drug attitude and adherence to anti-glaucoma medication.

    abstract:PURPOSE:The purpose of this study is to assess patient attitudes towards anti-glaucoma medication and their association with adherence, visual quality of life, and personality traits. MATERIALS AND METHODS:One hundred and forty-seven glaucoma patients were enrolled this study. The participants were divided into 'pharm...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2010.51.2.261

    authors: Hong S,Kang SY,Yoon JU,Kang U,Seong GJ,Kim CY

    更新日期:2010-03-01 00:00:00

  • Repolarization Heterogeneity of Magnetocardiography Predicts Long-Term Prognosis in Patients with Acute Myocardial Infarction.

    abstract:PURPOSE:Magnetocardiography (MCG) has been proposed as a noninvasive, diagnostic tool for risk-stratifying patients with acute myocardial infarction (AMI). This study evaluated whether MCG predicts long-term prognosis in AMI. MATERIALS AND METHODS:In 124 AMI patients (95 males, mean age 60±11 years), including 39 with...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2016.57.6.1339

    authors: Bang WD,Kim K,Lee YH,Kwon H,Park Y,Pak HN,Ko YG,Lee M,Joung B

    更新日期:2016-11-01 00:00:00

  • Diagnostic criteria of internal jugular phlebectasia in Korean children.

    abstract::Internal jugular phlebectasia (IJP) is a fusiform dilatation of the internal jugular vein (IJV), usually presented as a neck mass in children. Accurate diagnosis from carefully directed history, physical examination, and radiological study could result in lifesaving therapy. We performed our study to suggest possible ...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2002.43.3.329

    authors: Jeon CW,Choo MJ,Bae IH,Shin SO,Choi YS,Lee DW,Sim KH

    更新日期:2002-06-01 00:00:00

  • A case of urethral replacement with polytetrafluoro-ethylene graft for multiple anterior urethral strictures.

    abstract::Recently, the authors had an opportunity to treat a 73-year-old male patient who was transferred to our institution with problematic multiple strictures of the anterior urethra subsequent to a suprapubic cystostomy. A proximal urethra was reconstructed with scrotal skin, but due to the shortage of distal skin, as a la...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.1989.30.2.198

    authors: Choi HK,Chang SK

    更新日期:1989-01-01 00:00:00

  • Evaluating the allergic risk of genetically modified soybean.

    abstract::Genetically modified (GM) soybean (carrying the EPSPS transgene) is the most common GM food in Korea. In order to assess whether genetic modification increases the allergenic risk of soybeans, the allergenicity and IgE-reactive components of wild-type and GM soybean extracts were compared in allergic adults who had be...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2006.47.4.505

    authors: Kim SH,Kim HM,Ye YM,Kim SH,Nahm DH,Park HS,Ryu SR,Lee BO

    更新日期:2006-08-31 00:00:00

  • Tc-99m-ECD SPECT brain imaging in children with Tourette's syndrome.

    abstract::We undertook this study to assess the patterns of regional cerebral perfusion (RCP) with SPECT using Technetium- 99m-ethyl cysteinate dimer (Tc-99m-ECD) in children with Tourette's Syndrome (TS), and to compare these with the patterns in a group of normal controls. The study sample consisted of 38 children (7 to 14 ye...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2002.43.4.403

    authors: Diler RS,Reyhanli M,Toros F,Kibar M,Avci A

    更新日期:2002-08-01 00:00:00

  • Squamous cell carcinoma of the cornea.

    abstract::In this paper, a case of corneal squamous cell carcinoma is reported. Invasive squamous cell carcinoma of the cornea is a rare disorder and has not been previously described in the Korean literature. In this case, the invasive squamous cell carcinoma of the cornea was treated by complete excision and cryotherapy. No e...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2001.42.5.576

    authors: Shin HJ,Sohn JH,Goo YS,Park JY,Choi CH,Kim EK,Cho SH,Yoo NC,Roh JK

    更新日期:2001-10-01 00:00:00

  • High dose three-dimensional conformal boost using the real-time tumor tracking radiotherapy system in cervical cancer patients unable to receive intracavitary brachytherapy.

    abstract:PURPOSE:The purpose of this study is to evaluate the clinical results of treatment with a high dose of 3-dimensional conformal boost (3DCB) using a real-time tracking radiation therapy (RTRT) system in cervical cancer patients. MATERIALS AND METHODS:Between January 2001 and December 2004, 10 patients with cervical can...

    journal_title:Yonsei medical journal

    pub_type: 临床试验,杂志文章

    doi:10.3349/ymj.2010.51.1.93

    authors: Park HC,Shimizu S,Yonesaka A,Tsuchiya K,Ebina Y,Taguchi H,Katoh N,Kinoshita R,Ishikawa M,Sakuragi N,Shirato H

    更新日期:2010-01-01 00:00:00

  • Invasive papillary carcinoma of the breast presenting as post-traumatic recurrent hemorrhagic cysts.

    abstract::We report the sonographic features of an intracystic papillary carcinoma of the breast presenting as recurrent hemorrhagic cysts following trauma. A 56-year-old woman presented with palpable breast masses after a traumatic event; sonography showed multiple, well-defined, hemorrhagic cysts. Hemorrhagic fluid was evacua...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2006.47.4.575

    authors: Ko KH,Kim EK,Park BW

    更新日期:2006-08-31 00:00:00

  • Factor VLeiden and prothrombin G20210A gene polymorphisms in patients with coronary artery disease.

    abstract:PURPOSE:The precise molecular mechanisms culminating in coronary artery disease (CAD) are not well understood, despite a wealth of knowledge on predisposing risk factors and pathomechanisms. CAD and myocardial infarction (MI) are complex genetic diseases; neither the environment alone, nor a single gene, cause disease,...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.2008.49.2.237

    authors: Ercan B,Tamer L,Sucu N,Pekdemir H,Camsari A,Atik U

    更新日期:2008-04-30 00:00:00

  • Linear and whorled nevoid hypermelanosis with delayed psychomotor development.

    abstract::We report a case of a 25-month-old girl presented to us for the evaluation of a severe delayed psychomotor development who also has pigmentary abnormalities. Linear and whorled hyperpigmentations following Blaschko's lines were noticed on her entire body except on her face, palms, soles, eyes and mucous membranes, whi...

    journal_title:Yonsei medical journal

    pub_type: 杂志文章

    doi:10.3349/ymj.1996.37.4.290

    authors: Yim SY,Lee IY,Rah UW,Moon HW,Hahn SH,Lee ES,Yim HL

    更新日期:1996-08-01 00:00:00