Abstract:
OBJECTIVES:Postmenopausal osteoporosis (PMO) is a metabolic skeletal disorder with impaired bone density and bone quality in postmenopausal women. The aim of the present study was to investigate the correlation between neuropeptides, bone microstructure and pain threshold in ovariectomized (OVX) rats. METHODS:Female rats were randomly divided into the ovariectomized (OVX) group and the sham surgery (SHAM) group. Bone microstructure and immunocytochemistry for substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) in tibial and DRG were performed. Pain threshold was assessed at post-operative 11 weeks. Pearson correlation coefficients were calculated between neuropeptides, bone microstructure and pain threshold. RESULTS:Significant decreases in bone volume fraction (BV/TV) and trabecular number (Tb. N) but significant increases in trabecular spacing (Tb.Sp) were showed in OVX group. Mechanical pain threshold (MPT) in OVX group was significantly decreased. The MOD values for SP, CGRP and VIP of tibial in OVX group were significantly lower, whereas NPY, NPY1R and NPY2R were significantly higher. And SP, CGRP, VIP, NPY and NPY2R of DRG were significantly increased in OVX group, while NPY1R was significantly decreased. Correlation analysis showed that NPY, Y1R and Y2R in bone were negatively correlated with BV/TV. MPT was negatively correlated with NPY and Y2R in DRG, and positively correlated with Y1R in DRG. CONCLUSIONS:Our results suggested that SP, CGRP, VIP and NPY were involved in the osteoporotic bone microstructure and mechanical hypersensitivity in OVX rats, indicating the potential to utilize neuropeptides as novel therapeutic targets for PMO.
journal_name
Neuropeptidesjournal_title
Neuropeptidesauthors
Xie W,Li F,Han Y,Li Z,Xiao Jdoi
10.1016/j.npep.2019.101995subject
Has Abstractpub_date
2020-06-01 00:00:00pages
101995eissn
0143-4179issn
1532-2785pii
S0143-4179(19)30176-3journal_volume
81pub_type
杂志文章相关文献
NEUROPEPTIDES文献大全abstract::Germline ablation of VGF, a secreted neuronal, neuroendocrine, and endocrine peptide precursor, results in lean, hypermetabolic, and infertile adult mice that are resistant to diet-, lesion-, and genetically-induced obesity and diabetes (Hahm et al., 1999, 2002). To assess whether this phenotype is predominantly drive...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2016.12.005
更新日期:2017-08-01 00:00:00
abstract::Neural stem cells (NSC) could promote the repair after spinal cord transection (SCT), the underlying mechanism, however, still keeps to be defined. This study reported that NSC grafts significantly improved sensory and locomotor functions in adult rats with SCT in acute stage after injury. NSC could survive; different...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2012.06.001
更新日期:2013-02-01 00:00:00
abstract::Considerable evidence supports the existence of modulatory interactions between cholecystokinin and dopamine in the striatum. In order to explore further the nature of such interactions, the anatomical localization of CCK receptors in rat striatum was investigated autoradiographically following selective lesions. Infu...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(87)90014-x
更新日期:1987-08-01 00:00:00
abstract::Multiple sclerosis (MS) is an autoimmune disease of the central nervous system characterized by brain inflammation, demyelination and axonal loss. Neuropeptide Y (NPY) has a critical role in the maintenance of homeostasis in the immune system and coping of stress condition. In the current study we analyzed 188 patient...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2016.08.004
更新日期:2016-12-01 00:00:00
abstract::AtT-20 cells are known to synthesize two molecular weight forms of the prohormone converting enzyme PC1 with molecular masses of 87 and 66 kDa. In this study we have analyzed basal and stimulated secretion of these proteins. Western blot results show that basal secretion medium of cultured AtT-20 cells contained low c...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(93)90096-s
更新日期:1993-08-01 00:00:00
abstract::Immune system function has been shown to be under the influence of various neuromodulators and endocrine system peptides. This in vitro study describes the stimulatory effect of thyrotropin releasing hormone (thyroliberin, TRH) on thyrotropin (TSH) release from cultured human peripheral blood monocytes. The stimulator...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(93)90065-i
更新日期:1993-07-01 00:00:00
abstract::The effects of graded amounts of centrally injected [intracerebroventricular, i.c.v.] oxytocin [OXT] have been studied on the steady-state level and pargyline-induced accumulation of serotonin [5-HT] in the hypothalamus, mesencephalon and dorsal hippocampus of rats. OXT only weakly affected the steady-state level of 5...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(84)90125-2
更新日期:1984-03-01 00:00:00
abstract::The effects of the GABAergic drugs nipecotic acid, Gabrene, baclofen and metatolylcarbamide (MTC), when given alone or in combination at subthreshold doses with AT II also at a subthreshold dose effective on PTZ-kindling in mice were studied. PTZ-kindling was provoked by intraperitoneal (i.p.) injections of PTZ (40 mg...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(95)90071-3
更新日期:1995-01-01 00:00:00
abstract::To find out, whether microheterogeneity of neurophysins is due to aging of neurosecretory granules we investigated neurophysin proteins of porcine origin from newly formed and aged granules, as well as from two different extraction methods from whole posterior pituitary glands by high performance liquid chromatography...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(83)90003-3
更新日期:1983-12-01 00:00:00
abstract::Quantitative evaluation of octadecaneuropeptide-like immunoreactivity (ODN-Li) was carried out in hippocampus, cortex and cerebellum of group-reared or three month isolated male Wistar rats, whether muricidal or not. ODN-Li was lower in cortex and cerebellum of isolated animals, (mainly in muricidal) than of group-rea...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(91)90116-z
更新日期:1991-07-01 00:00:00
abstract::The intracerebrally administered dynorphin-B produced not only an antinociceptive response but a motor dysfunction such as "barrel-rolling", circling, jumping or ataxia in mice. These two effects were observed in a same dose range. Both responses were also produced by a nonopioid fragment, des-Tyr-dynorphin-B. Bestati...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(89)90082-6
更新日期:1989-05-01 00:00:00
abstract::Cholecystokinin-B (CCK-8) is an octapeptide that was initially described in the gastrointestinal tract. Recent studies have shown that this peptide also has an extensive distribution in the central nervous system, including the cerebellum of the opossum. In addition to the protein, binding sites for CCK-8 also have be...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/s0143-4179(96)90035-9
更新日期:1996-12-01 00:00:00
abstract::Pheochromocytoma (PC-12) cells exposed to nerve growth factor (NGF) acquire a sympathetic neuron-like phenotype. This NGF-response is blocked by methylation inhibitors and can be mimicked by the farnesylated methylated small GTP-binding protein p21ras. The implicated involvement of prenylation, methylation and a small...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(93)90036-a
更新日期:1993-01-01 00:00:00
abstract::Our previous studies showed the modulation of cocaine and amphetamine regulated transcript (CART) positive neurons and CART mRNA by adrenalectomy and corticosterone replacement in hypothalamic nuclei of male rat brain. More recently, we have shown by CART immunohistochemistry that restraint and forced swim (FS) stress...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2006.02.001
更新日期:2006-06-01 00:00:00
abstract::Pyroglutamyl peptidase I activity from soluble and membrane-bound fractions of rat brain homogenates is inhibited by the presence of sodium deoxycholate but not by triton X-100. Biobeads SM2, a polystyrene adsorbent reported to be useful in removing detergents from aqueous solutions, inhibits enzymatic activity in bot...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(95)90092-6
更新日期:1995-08-01 00:00:00
abstract::Both endothelins and corticotropin releasing factor (CRF) appear in capsaicin-sensitive neurons. We have investigated the effects of human endothelin-1 (ET-1) and CRF in the guinea-pig ileum longitudinal and circular preparations and sought for ways of specific antagonism. With the aid of tachyphylaxis to capsaicin (i...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/s0143-4179(03)00048-9
更新日期:2003-08-01 00:00:00
abstract::Since there are conflicting reports regarding the effects of somatostatin (SS) on cyclic AMP levels in astrocytes derived from rat cerebral cortex and, to date, the SS binding to mature astrocytes is unknown, the present study has determined SS binding and its effect on cyclic AMP accumulation in a fresh astrocyte-ric...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(92)90002-e
更新日期:1992-09-01 00:00:00
abstract::Chronic stress induces the activation of hippocampal microglia, which produces many inflammatory mediators and mediates the occurrence of depression. Two phenotypes of microglia polarization, the classical M1 and alternative M2, play important regulatory roles in neuroinflammation and are involved in the occurrence an...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2020.102006
更新日期:2020-06-01 00:00:00
abstract::Cerebral amyloid-beta protein (Abeta) deposition and associated neuroinflammation and apoptosis are increasingly recognized as an important component leading to cognitive impairment in Alzheimer's disease (AD). Humanin (HN) and its derivative, S14G-HN (HNG), are best known for their ability to suppress neuronal death ...
journal_title:Neuropeptides
pub_type: 杂志文章,收录出版
doi:10.1016/j.npep.2008.08.004
更新日期:2008-10-01 00:00:00
abstract::In situ hybridisation histochemistry was used to study the effect of intra-peritoneal reserpine administration on messenger RNA (mRNA) levels in the locus coeruleus (LC) of the rat. 48h after injection, levels of mRNA encoding tyrosine hydroxylase (TH) and neuropeptide Y (NPY) in the LC increased to approximately 250%...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(91)90105-r
更新日期:1991-03-01 00:00:00
abstract::Gestational cocaine treatment in rat dams results in decreased oxytocin (OT) levels, up-regulated oxytocin receptor (OTR) binding density and decreased receptor affinity in the whole amygdala, all concomitant with a significant increase in maternal aggression on postpartum day six. Rat dams with no gestational drug tr...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2006.03.002
更新日期:2006-06-01 00:00:00
abstract::Dermorphin (Tyr-D-Ala-Phe-Gly-Tyr-Pro-Ser-NH2), a naturally occurring peptide isolated from arboreal frog skin, is endowed with outstanding structural and biological features. It has no structural community with the sequence of mammalian opioid peptides and is a unique example of a D-aminoacid containing peptide which...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(89)90021-8
更新日期:1989-01-01 00:00:00
abstract::We have described the binding of [125I]endothelin-1 (ET-1) to cultured rat cerebellar granule cells. Binding of [125I]ET-1 was specific, saturable, and time-dependent. Scatchard analysis of saturation binding data indicated a single class of high affinity binding site with a KD of 95 pM, and a Bmax of 8110 receptors/c...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(91)90005-4
更新日期:1991-02-01 00:00:00
abstract::Regulation of cholecystokinin (CCK) expression was studied in the human neuroepithelioma cell line SK-N-MCIXC and the rat medullary thyroid carcinoma cell line WE 4/2. The cells were treated with the beta-adrenergic agonist isoproterenol and retinoic acid, a natural derivative of vitamin A, which plays a role in cell ...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(95)90064-0
更新日期:1995-10-01 00:00:00
abstract::Acid extracts from mouse Neuroblastoma x rat Glioma hybrid cells have been purified by means of Sep-Pak C-18 and fractionated by high performance liquid chromatography. Each fraction has been submitted to a sensitive beta-endorphin radioimmunoassay and an immunoreactivity peak at camel beta-endorphin retention time wa...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(85)90130-1
更新日期:1985-03-01 00:00:00
abstract::The present study was planned to evaluate role of tachykinins (TKs) and neurokinin (NK) receptors in the regulation of gastric motility in sheep. We examined the effects of intravenous (i.v.) injection of neurokinin A (NKA) and substance P (SP) on motility of the rumen, omasum, and abomasum in conscious sheep and the ...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2012.07.005
更新日期:2013-02-01 00:00:00
abstract::Humanin (HN) is an endogenous 24-residue peptide. A highly potent HN derivative, S14G-HN, which has a substitution of serine 14 to glycine, reduced amyloid burden and suppressed cognitive impairment in a mouse model of Alzheimer's disease. S14G-HN also suppressed amnesia induced by a muscarinic receptor antagonist in ...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2016.10.008
更新日期:2017-04-01 00:00:00
abstract::In order to study the morphological localization of the endogenous benzodiazepine ligand octadecaneuropeptide (ODN) in rat brain, we have developed antibodies against this peptide. Using a radioimmunoassay for ODN, we have observed that synthetic ODN and serial dilutions of several brain areas gave parallel displaceme...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(90)90155-r
更新日期:1990-01-01 00:00:00
abstract::In an attempt to determine the localization of the messenger RNA (mRNA) encoding somatostatin in the rat retina, we studied Sprague-Dawley rats by in situ hybridization histochemistry using radiolabelled oligodeoxyribonucleotides complementary for rat somatostatin mRNA. Among the layers of retina, we found specific la...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(90)90135-l
更新日期:1990-09-01 00:00:00
abstract::There is now considerable evidence that putative neuropeptides are involved in epileptogenic processes. Neurotensin (NT), which affects neuronal excitability in many parts of the CNS, is potentially relevant to the kindling phenomenon, but has not previously been studied in the kindling model of epilepsy. This study c...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(88)90014-5
更新日期:1988-02-01 00:00:00