Abstract:
:Message RNA poly(A) tails are vital for their function and regulation. However, the full-length sequence of mRNA isoforms with their poly(A) tails remains undetermined. Here, we develop a method at single-cell level sensitivity that enables quantification of poly(A) tails along with the full-length cDNA while reading non-adenosine residues within poly(A) tails precisely, which we name poly(A) inclusive RNA isoform sequencing (PAIso-seq). Using this method, we can quantify isoform specific poly(A) tail length. More interestingly, we find that 17% of the mRNAs harbor non-A residues within the body of poly(A) tails in mouse GV oocytes. We show that PAIso-seq is sensitive enough to analyze single GV oocytes. These findings will not only provide an accurate and sensitive tool in studying poly(A) tails, but also open a door for the function and regulation of non-adenosine modifications within the body of poly(A) tails.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Liu Y,Nie H,Liu H,Lu Fdoi
10.1038/s41467-019-13228-9subject
Has Abstractpub_date
2019-11-22 00:00:00pages
5292issue
1issn
2041-1723pii
10.1038/s41467-019-13228-9journal_volume
10pub_type
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