Abstract:
:Protein ubiquitylation participates in a number of essential cellular processes including signal transduction and transcription, often by initiating the degradation of specific substrates through the 26S proteasome. Within the ubiquitin-proteasome system, deubiquitylating enzymes (DUBs) not only help generate and maintain the supply of free ubiquitin monomers, they also directly control functions and activities of specific target proteins by modulating the pool of ubiquitylated species. Ubiquitin carboxyl-terminal hydrolases (UCHs) belong to an enzymatic subclass of DUBs, and are represented by three members in Arabidopsis, UCH1, UCH2 and UCH3. UCH1 and UCH2 influence auxin-dependent developmental pathways in Arabidopsis through their deubiquitylation activities, whereas biological and enzymatic functions of UCH3 remain unclear. Here, we demonstrate that Arabidopsis UCH3 acts to maintain the period of the circadian clock at high temperatures redundantly with UCH1 and UCH2. Whereas single uch1, uch2 and uch3 mutants have weak circadian phenotypes, the triple uch mutant displays a drastic lengthening of period at high temperatures that is more extreme than the uch1 uch2 double mutant. UCH3 also possesses a broad deubiquitylation activity against a range of substrates that link ubiquitin via peptide and isopeptide linkages. While the protein target(s) of UCH1-3 are not yet known, we propose that these DUBs act on one or more factors that control period length of the circadian clock through removal of their bound ubiquitin moieties, thus ensuring that the clock oscillates with a proper period even at elevated temperatures.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Hayama R,Yang P,Valverde F,Mizoguchi T,Furutani-Hayama I,Vierstra RD,Coupland Gdoi
10.1038/s41598-019-53229-8subject
Has Abstractpub_date
2019-11-19 00:00:00pages
17030issue
1issn
2045-2322pii
10.1038/s41598-019-53229-8journal_volume
9pub_type
杂志文章abstract::The objective of this study was to examine the relationship between sleep duration and ischemic and hemorrhagic stroke in a community-based cohort. The current analysis included 95,023 Chinese participants who were free of stroke at the baseline survey (2006-2007). Cox proportional hazards models were used to calculat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33664
更新日期:2016-09-16 00:00:00
abstract::De novo mutations (DNMs), including germinal and postzygotic mutations (PZMs), are a strong source of causality for Autism Spectrum Disorder (ASD). However, the biological processes involved behind them remain unexplored. Our aim was to detect DNMs (germinal and PZMs) in a Spanish ASD cohort (360 trios) and to explore...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79412-w
更新日期:2021-01-11 00:00:00
abstract::The applications of spin dynamos, which could potentially power complex nanoscopic devices, have so far been limited owing to their extremely low energy conversion efficiencies. Here, we present a unique plasmonic diabolo cavity (PDC) that dramatically improves the spin rectification signal (enhancement of more than t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05634-0
更新日期:2017-07-13 00:00:00
abstract::We characterize the changes in chromatin structure, DNA methylation and transcription during and after homologous DNA repair (HR). We find that HR modifies the DNA methylation pattern of the repaired segment. HR also alters local histone H3 methylation as well chromatin structure by inducing DNA-chromatin loops connec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33222
更新日期:2016-09-15 00:00:00
abstract::The function of most long noncoding RNAs (lncRNAs) is unknown. However, recent studies reveal important roles of lncRNAs in regulating cancer-related pathways. Human antisense lncRNA-NKX2-1-AS1 partially overlaps the NKX2-1/TTF1 gene within chromosomal region 14q13.3. Amplification of this region and/or differential e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32793-5
更新日期:2018-09-26 00:00:00
abstract::Multiple sclerosis is a chronic, autoimmune and neurodegenerative disease affecting multiple functional systems and resulting in motor impairments associated with muscle weakness and lack of movement coordination. We quantified upper limb motor deficits with a robot-based assessment including behavioral and muscle syn...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20343-y
更新日期:2018-02-01 00:00:00
abstract::The synthesis of highly biocompatible polymers is important for modern biotechnologies and medicine. Here, we report a unique process based on a two-step high-pressure ramp (HPR) for the ultrafast and efficient bulk polymerization of 2-(hydroxyethyl)methacrylate (HEMA) at room temperature without photo- and thermal ac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18244
更新日期:2015-12-16 00:00:00
abstract::The morphology of hexagonal phase NaYF4:Er(3+) nanorods synthesized by hydrothermal method changed greatly after a continuing calcination, along with a phase transformation to cubic phase. Photoluminescence (PL) spectra indicated that mid-infrared (MIR) emission was obtained in both hexagonal and cubic phase NaYF4:Er(...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29871
更新日期:2016-07-25 00:00:00
abstract::Spin injection, manipulation and detection are the integral parts of spintronics devices and have attracted tremendous attention in the last decade. It is necessary to judiciously choose the right combination of materials to have compatibility with the existing semiconductor technology. Conventional metallic magnets w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05588
更新日期:2014-07-07 00:00:00
abstract::Si holds great promise as an alloying anode material for Li-ion batteries with improved energy density because of its high theoretical specific capacity and favorable operation voltage range. However, the large volume expansion of Si during electrochemical reaction with Li and the associated adverse effects strongly l...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13261-y
更新日期:2017-10-12 00:00:00
abstract::The freshwater crustacean Daphnia is known for its ability to develop inducible morphological defences that thwart predators. These defences are developed only in the presence of predators and are realized as morphological shape alterations e.g. 'neckteeth' in D. pulex and 'crests' in D. longicephala. Both are discuss...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09649-5
更新日期:2017-08-29 00:00:00
abstract::Coastal ecosystems play significant ecological and economic roles but are threatened and facing decline. Microbes drive various biogeochemical processes in coastal ecosystems. Tidal flats are critical components of coastal ecosystems; however, the structure and function of microbial communities in tidal flats are poor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36550
更新日期:2016-11-08 00:00:00
abstract::Rice (Oryza sativa L.) is one of the essential staple food crops and tillering, panicle branching and grain filling are three important traits determining the grain yield. Although miRNAs have been reported being regulating yield, no study has systematically investigated how miRNAs differentially function in high and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26438-w
更新日期:2018-05-31 00:00:00
abstract::The brain-derived neurotrophic factor BDNF plays a critical role in neuronal development and the induction of L-LTP at glutamatergic synapses in several brain regions. However, the cellular and molecular mechanisms underlying these BDNF effects have not been firmly established. Using in vitro cultures of cortical neur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14778
更新日期:2015-10-06 00:00:00
abstract::Advanced fibrosis and portal hypertension influence short-term mortality. Lipocalin 2 (LCN2) regulates infection response and increases in liver injury. We explored the role of intrahepatic LCN2 in human alcoholic hepatitis (AH) with advanced fibrosis and portal hypertension and in experimental mouse fibrosis. We foun...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72172-7
更新日期:2020-09-23 00:00:00
abstract::The incidence of ovarian cancer dramatically increases in early menopause but the factors contributing to cancer onset are unclear. Most ovarian cancers originate in the fallopian tube with subsequent implantation of malignant cells into the ovary. However, the events and conditions that lead to cancer cell implantati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30261-8
更新日期:2018-08-17 00:00:00
abstract::We study universal uncertainty relations and present a method called joint probability distribution diagram to improve the majorization bounds constructed independently in [Phys. Rev. Lett. 111, 230401 (2013)] and [J. Phys. A. 46, 272002 (2013)]. The results give rise to state independent uncertainty relations satisfi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35735
更新日期:2016-10-24 00:00:00
abstract::Alternatives to antibiotics for improving productivity and maintaining the health of livestock health are urgently needed. The scope of this research was conducted to investigate the effects of two alternatives (Bacillus licheniformis and Saccharomyces cerevisiae) to monensin on growth performance, antioxidant capacit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35081-4
更新日期:2018-11-12 00:00:00
abstract::Dendritic spines are the postsynaptic sites of most excitatory synapses in the brain, and spine enlargement and shrinkage give rise to long-term potentiation and depression of synapses, respectively. Because spine structural plasticity is accompanied by remodeling of actin scaffolds, we hypothesized that the filamento...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32897
更新日期:2016-09-06 00:00:00
abstract::To date, there is only scarce evidence for a considerable association of subjective and objective stress measures, which might be attributable to method bias (e.g., confounding) and/or asynchrony of their temporal changes. To validate different subjective stress measures by a physiological measure of long-term stress ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37526-2
更新日期:2019-01-31 00:00:00
abstract::Lipases are interfacially activated enzymes that catalyze the hydrolysis of ester bonds and constitute prime candidates for industrial and biotechnological applications ranging from detergent industry, to chiral organic synthesis. As a result, there is an incentive to understand the mechanisms underlying lipase activi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52539-1
更新日期:2019-11-07 00:00:00
abstract::Nanostructured (NS) and ultrafine-grained (UFG) materials have high strength and relatively low ductility. Their toughness has not been comprehensively investigated. Here we report the Charpy impact behavior and the corresponding microstructural evolutions in UFG Cu with equi-axed and elongated grains which were prepa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44783
更新日期:2017-03-17 00:00:00
abstract::Doubly curved nanofiber-reinforced optically transparent composites with low thermal expansion of 15 ppm/k are prepared by hot pressing vacuum-filtered Pickering emulsions of hydrophobic acrylic resin monomer, hydrophilic chitin nanofibers and water. The coalescence of acrylic monomer droplets in the emulsion is preve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16421
更新日期:2015-11-10 00:00:00
abstract::Hydrogels are being extensively used for three-dimensional immobilization and culture of cells in fundamental biological studies, biochemical processes, and clinical treatments. However, it is still a challenge to support viability and regulate phenotypic activities of cells in a structurally stable gel, because the g...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08948
更新日期:2015-03-10 00:00:00
abstract::Phospholipase A2-like (PLA2-like) proteins contribute to the development of muscle necrosis in Viperidae snake bites and are not efficiently neutralized by current antivenom treatments. The toxic mechanisms of PLA2-like proteins are devoid of catalytic activity and not yet fully understood, although structural and fun...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15614-z
更新日期:2017-11-14 00:00:00
abstract::Diabetic cardiomyopathy is a distinct pathology characterized by early emergence of diastolic dysfunction. Increased cardiovascular risk associated with diabetes is more marked for women, but an understanding of the role of diastolic dysfunction in female susceptibility to diabetic cardiomyopathy is lacking. To invest...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20703-8
更新日期:2018-02-05 00:00:00
abstract::In this retrospective study, we investigated the impact of diabetes mellitus (DM) on patients with head and neck cancer (HNC) undergoing concurrent chemoradiotherapy (CCRT). We analyzed the demographic and clinical characteristics, treatment tolerance, and toxicities of patients with HNC undergoing primary or adjuvant...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64844-1
更新日期:2020-05-07 00:00:00
abstract::The mechanism whereby lactic acid bacteria extend the lifespan of Caenorhabditis elegans has previously been elucidated. However, the role of Weissella species has yet not been studied. We show that Weissella koreensis and Weissella cibaria significantly (p < 0.05) extend the lifespan of C. elegans compared with Esche...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17128
更新日期:2015-11-25 00:00:00
abstract::Mendelian randomisation studies from Asia suggest detrimental influences of alcohol on cardiovascular risk factors, but such associations are observed mainly in men. The absence of associations of genetic variants (e.g. rs671 in ALDH2) with such risk factors in women - who drank little in these populations - provides ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18422
更新日期:2015-12-21 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-62901-3
更新日期:2020-04-20 00:00:00