Abstract:
:A microfluidic laminar flow cell (LFC) forms an indispensable component in single-molecule experiments, enabling different substances to be delivered directly to the point under observation and thereby tightly controlling the biochemical environment immediately surrounding single molecules. Despite substantial progress in the production of such components, the process remains relatively inefficient, inaccurate and time-consuming. Here we address challenges and limitations in the routines, materials and the designs that have been commonly employed in the field, and introduce a new generation of LFCs designed for single-molecule experiments and assembled using additive manufacturing. We present single- and multi-channel, as well as reservoir-based LFCs produced by 3D printing to perform single-molecule experiments. Using these flow cells along with optical tweezers, we show compatibility with single-molecule experiments including the isolation and manipulation of single DNA molecules either attached to the surface of a coverslip or as freely movable DNA dumbbells, as well as direct observation of protein-DNA interactions. Using additive manufacturing to produce LFCs with versatility of design and ease of production allow experimentalists to optimize the flow cells to their biological experiments and provide considerable potential for performing multi-component single-molecule experiments.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ahmadi A,Till K,Hafting Y,Schüttpelz M,Bjørås M,Glette K,Tørresen J,Rowe AD,Dalhus Bdoi
10.1038/s41598-019-53151-zsubject
Has Abstractpub_date
2019-11-14 00:00:00pages
16784issue
1issn
2045-2322pii
10.1038/s41598-019-53151-zjournal_volume
9pub_type
杂志文章abstract::Rodents are important reservoir hosts of many important zoonotic viruses. The family Picornaviridae contains clinically important pathogens that infect humans and animals, and increasing numbers of rodent picornaviruses have recently been associated with zoonoses. We collected 574 pharyngeal and anal swab specimens fr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34381
更新日期:2016-09-29 00:00:00
abstract::The majority of oligomeric proteins form clusters which have rotational or dihedral symmetry. Despite the many advantages of symmetric packing, protein oligomers are only nearly symmetric, and the origin of this phenomenon is still in need to be fully explored. Here we apply near-symmetry analyses by the Continuous Sy...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65097-8
更新日期:2020-05-20 00:00:00
abstract::The development of the peripheral nervous system relies on long-distance signaling from target organs back to the soma. In sympathetic neurons, this long-distance signaling is mediated by target derived Nerve Growth Factor (NGF) interacting with its axonal receptor, TrkA. This ligand receptor complex internalizes into...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23036-8
更新日期:2018-03-16 00:00:00
abstract::The expression of legumain which has been shown overexpressed in patients with metastatic gastric cancer is positively correlated to both disease progression and outcome, and negatively correlated to microRNA (miR)-3978 expression. The RNA-binding protein, poly r(C) binding protein 1 (PCBP1) was the most downregulated...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15448-9
更新日期:2017-11-14 00:00:00
abstract::The molecular dissociation constant, Kd, is a well-established parameter to quantitate the affinity of protein-protein or other molecular interactions. Recently, we reported the theoretical basis and experimental procedure for Kd determination using a quantitative FRET method. Here we report a new development of Kd de...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35535-9
更新日期:2019-02-14 00:00:00
abstract::Trastuzumab combined with chemotherapy is standard of care for HER2 positive advanced gastro-esophageal cancers. The reported prevalence of HER2 discordance between primary tumors and corresponding metastases varies, hampering uniform patient selection for HER2 targeted therapy. This meta-analysis explores the influen...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-017-03304-9
更新日期:2017-06-09 00:00:00
abstract::Microbial life below the seafloor has changed over geological time, but these changes are often not obvious, as they are not recorded in the sediment. Sulphur (S) isotope values in pyrite extracted from a Plio- to Holocene sequence of the Peru Margin (Ocean Drilling Program, ODP, Site 1229) show a down-core pattern th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36943-7
更新日期:2019-01-24 00:00:00
abstract::Split bull's eye (SBE) antennas exhibit much larger extraordinary optical transmission and strong polarization dependence rather than bull's eye (BE) antennas in the infrared range due to the introduced sub-wavelength slit. Here, we demonstrate a dual-split bull's eye (DSBE) antenna, which consists of two sub-waveleng...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39106
更新日期:2016-12-19 00:00:00
abstract::Few-layered transition metal dichalcogenides (TMDs) are known as true two-dimensional materials, with excellent semiconducting properties and strong light-matter interaction. Thus, TMDs are attractive materials for semitransparent and flexible solar cells for use in various applications. Hoewver, despite the recent pr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12287-6
更新日期:2017-09-20 00:00:00
abstract::The growth of epitaxial Si nanowires by a metal-catalyst-free process has been investigated as an alternative to the more common metal-catalyzed vapor-liquid-solid process. The well-aligned Si nanowires are successfully grown on a (111)-oriented Si substrate without any metal catalysts by a thermal treatment using sil...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30608
更新日期:2016-07-28 00:00:00
abstract::In a haptic search task, one has to detect the presence of a target among distractors using the sense of touch. A salient target can be detected faster than a non-salient target. However, little is known about the exploration strategies that are used, especially in 3D search tasks where items are held in the hand. In ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06254
更新日期:2014-09-03 00:00:00
abstract::Surfactants during routine washing have a tremendous effect on lipid loss from hair. This study aims to understand the loss of lipids from hair upon contact with surfactants and develop a way to prevent the lipid loss. The change in lipid levels depends on the relative hydrophobicity of the lipid. We herein propose th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46370-x
更新日期:2019-07-08 00:00:00
abstract::Global warming is projected to continue, leading to intense fluctuations in precipitation and heat waves and thereby affecting the productivity and the relevant biological processes of grassland ecosystems. Here, we determined the functional responses to warming and altered precipitation in both typical and desert ste...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34801
更新日期:2016-10-10 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-62742-0
更新日期:2020-04-10 00:00:00
abstract::Long non-coding RNAs (lncRNAs) regulate expression of protein-coding genes in cis through chromatin modifications including DNA methylation. Here we interrogated whether lncRNA genes may regulate transcription and methylation of their flanking or overlapping protein-coding genes in livers of mice exposed to a 12-week ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29993-4
更新日期:2018-08-01 00:00:00
abstract::Hereditary Parkinson's disease is commonly caused by mutations in the protein kinase PINK1 or the E3 ubiquitin ligase Parkin, which function together to eliminate damaged mitochondria. PINK1 phosphorylates both Parkin and ubiquitin to stimulate ubiquitination of dozens of proteins on the surface of the outer mitochond...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33019
更新日期:2016-09-08 00:00:00
abstract::Preventing ischemia-related cell damage is a priority when preserving tissue for transplantation. Perfusion protocols have been established for a variety of applications and proven to be superior to procedures used in clinical routine. Extracorporeal perfusion of muscle tissue though cumbersome is highly desirable sin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11956
更新日期:2015-07-06 00:00:00
abstract::The synthetic Angiotensin Converting Enzyme (ACE) inhibitors have side effects and hence demands for natural ACE inhibitors have been rising. The aim of this study is to purify and introduce natural ACE inhibitors extracted from Zizyphus jujuba fruits. Proteins from Zizyphus jujuba were lysed by trypsin, papain and th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60972-w
更新日期:2020-03-04 00:00:00
abstract::Changes in the stability, hydrogen diffusion, and mechanical properties of the NbH phases from Ni-doping was studied by using first-principles methods. The calculation results reveal that the single H atom adsorption is energetically favorable at the tetrahedral interstitial site (TIS) and octahedral interstitial site...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06658-2
更新日期:2017-07-26 00:00:00
abstract::In spite of many compounds identified as antifibrotic in preclinical studies, pulmonary fibrosis remains a life-threatening condition for which highly effective treatment is still lacking. Towards improving the success-rate of bench-to-bedside translation, we investigated in vivo µCT-derived biomarkers to repeatedly q...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73056-6
更新日期:2020-09-30 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-57850-w
更新日期:2020-01-21 00:00:00
abstract::Microalgal harvesting and dewatering are the main bottlenecks that need to be overcome to tap the potential of microalgae for production of valuable compounds. Water surface-floating microalgae form robust biofilms, float on the water surface along with gas bubbles entrapped under the biofilms, and have great potentia...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47612-8
更新日期:2019-08-01 00:00:00
abstract::We propose a reflection type metal-insulator-metal (MIM) metasurface composed of hybrid nano-antennas for comprehensive spatial engineering of the properties of optical fields. The capability of such structure is illustrated in the design of a device that can be used to produce a radially polarized vectorial beam for ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29626
更新日期:2016-07-15 00:00:00
abstract::Targeting epigenetic reader proteins by small molecule inhibitors represents a new therapeutic concept in autoimmune diseases such as rheumatoid arthritis (RA). Although inhibitors targeting bromodomain protein 1 (BRD1) are in development, the function of BRD1 has hardly been studied. We investigated the therapeutic p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29127-w
更新日期:2018-07-24 00:00:00
abstract::The aim of this study was to identify novel plasma metabolic signatures with possible relevance during multiple myeloma (MM) development and progression. A biochemical quantitative phenotyping platform based on targeted electrospray ionization tandem mass spectrometry technology was used to aid in the identification o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75862-4
更新日期:2020-12-14 00:00:00
abstract::In order to improve the accuracy and reliability of micropipetting, a method of micro-pipette detection and calibration combining the dynamic pressure monitoring in pipetting process and quantitative identification of pipette volume in image processing was proposed. Firstly, the normalized pressure model for the pipet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24145-0
更新日期:2018-04-10 00:00:00
abstract::Quantum bits (qubits) are at the heart of quantum information processing schemes. Currently, solid-state qubits, and in particular the superconducting ones, seem to satisfy the requirements for being the building blocks of viable quantum computers, since they exhibit relatively long coherence times, extremely low diss...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29374
更新日期:2016-07-12 00:00:00
abstract::During chronic cerebral hypoperfusion (CCH), the cerebral blood flow gradually decreases, leading to cognitive impairments and neurodegenerative disorders, such as vascular dementia. The reduced oxygenation, energy supply induced metabolic changes, and insufficient neuroplasticity could be reflected in the synaptic pr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72868-w
更新日期:2020-09-29 00:00:00
abstract::Predicting function-related amino acids in proteins with unknown function or unknown allosteric binding sites in drug-targeted proteins is a task of paramount importance in molecular biomedicine. In this paper we introduce a simple, light and computationally inexpensive structure-based method to identify catalytic sit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14874
更新日期:2015-10-08 00:00:00
abstract::The methylotrophic yeast Komagataella phaffii (Pichia pastoris) has been developed into a highly successful system for heterologous protein expression in both academia and industry. However, overexpression of recombinant protein often leads to severe burden on the physiology of K. phaffii and triggers cellular stress....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16577-x
更新日期:2017-11-24 00:00:00