Abstract:
:Previous studies in model eukaryotes have demonstrated that phosphorylation of heterochromatin protein 1 (HP1) is important for dynamically regulating its various functions. However, in the malaria parasite Plasmodium falciparum both the function of HP1 phosphorylation and the identity of the protein kinases targeting HP1 are still elusive. In order to functionally analyze phosphorylation of P. falciparum HP1 (PfHP1), we first mapped PfHP1 phosphorylation sites by liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of native PfHP1, which identified motifs from which potential kinases could be predicted; in particular, several phosphorylated residues were embedded in motifs rich in acidic residues, reminiscent of targets for P. falciparum casein kinase 2 (PfCK2). Secondly, we tested recombinant PfCK2 and a number of additional protein kinases for their ability to phosphorylate PfHP1 in in vitro kinase assays. These experiments validated our prediction that PfHP1 acts as a substrate for PfCK2. Furthermore, LC-MS/MS analysis showed that PfCK2 phosphorylates three clustered serine residues in an acidic motif within the central hinge region of PfHP1. To study the role of PfHP1 phosphorylation in live parasites we used CRISPR/Cas9-mediated genome editing to generate a number of conditional PfHP1 phosphomutants based on the DiCre/LoxP system. Our studies revealed that neither PfCK2-dependent phosphorylation of PfHP1, nor phosphorylation of the hinge domain in general, affect PfHP1's ability to localize to heterochromatin, and that PfHP1 phosphorylation in this region is dispensable for the proliferation of P. falciparum blood stage parasites.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Bui HTN,Niederwieser I,Bird MJ,Dai W,Brancucci NMB,Moes S,Jenoe P,Lucet IS,Doerig C,Voss TSdoi
10.1038/s41598-019-53325-9subject
Has Abstractpub_date
2019-11-13 00:00:00pages
16720issue
1issn
2045-2322pii
10.1038/s41598-019-53325-9journal_volume
9pub_type
杂志文章abstract::We developed an optimal noninvasive index comprising routine laboratory parameters for predicting cirrhosis in chronic hepatitis B (CHB) and chronic hepatitis C (CHC) patients. This study included 992 CHB patients and 1,284 CHC patients who received liver biopsy. We developed the new index, named modified Fibrosis-4 (...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03589-w
更新日期:2017-06-12 00:00:00
abstract::Much of atmospheric water originates from transpiration, the process by which plants release H2O from pores, known as stomata, that simultaneously intake CO2 for photosynthesis. Controlling stomatal aperture can regulate the extent of water transport in response to dynamic environmental factors including osmotic stres...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32394
更新日期:2016-08-31 00:00:00
abstract::Male infertility due to spermatogenesis defects affects millions of men worldwide. However, the genetic etiology of the vast majority remains unclear. Here we describe three men with primary infertility due to multiple morphological abnormalities of the sperm flagella (MMAF) from two unrelated Han Chinese families. We...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-019-52436-7
更新日期:2019-11-01 00:00:00
abstract::Negative hemodynamic response has been widely reported in blood oxygenation level-dependent (BOLD) functional magnetic resonance imaging studies, however its origin is still controversial. Optical intrinsic signal (OIS) imaging can be used to study brain activity by simultaneously recording hemodynamic signals at diff...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21627
更新日期:2016-02-10 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-46785-6
更新日期:2019-07-17 00:00:00
abstract::Understanding and control of heat dissipation is an important challenge in nanoelectronics wherein field-accelerated hot carriers in current-carrying ballistic systems release a large part of the kinetic energy into external bulk phonon baths. Here we report on a physical mechanism of this remote heat dissipation and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26203-z
更新日期:2018-05-18 00:00:00
abstract::High content of organic matter in the downstream of watersheds underscored the severity of non-point source (NPS) pollution. The major objectives of this study were to characterize and quantify dissolved organic matter (DOM) in watersheds affected by NPS pollution, and to apply self-organizing map (SOM) and parallel f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16079
更新日期:2015-11-03 00:00:00
abstract::SLC7A10 (Asc-1) is a sodium-independent amino acid transporter known to facilitate transport of a number of amino acids including glycine, L-serine, L-alanine, and L-cysteine, as well as their D-enantiomers. It has been described as a neuronal transporter with a primary role related to modulation of excitatory glutama...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35592
更新日期:2016-10-19 00:00:00
abstract::Survival analyses for malignancies, including renal cell carcinoma (RCC), have primarily been conducted using the Cox proportional hazards (CPH) model. We compared the random survival forest (RSF) and DeepSurv models with the CPH model to predict recurrence-free survival (RFS) and cancer-specific survival (CSS) in non...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80262-9
更新日期:2021-01-13 00:00:00
abstract::Delay embedding-a method for reconstructing dynamical systems by delay coordinates-is widely used to forecast nonlinear time series as a model-free approach. When multivariate time series are observed, several existing frameworks can be applied to yield a single forecast combining multiple forecasts derived from vario...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-57255-4
更新日期:2020-01-20 00:00:00
abstract::Precise mitotic spindle orientation is essential for both cell fate and tissue organization while defects in this process are associated with tumorigenesis and other diseases. In most animal cell types, the dynein motor complex is anchored at the cell cortex and exerts pulling forces on astral microtubules to position...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24682-8
更新日期:2018-04-20 00:00:00
abstract::The striatum plays important motor, associative and cognitive roles in brain functions. However, the rodent dorsolateral (the primate putamen) and dorsomedial (the primate caudate nucleus) striatum are not anatomically separated, making it difficult to distinguish their functions. By contrast, anatomical separation ex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35809
更新日期:2016-11-02 00:00:00
abstract::The CoCrMo matrix composites with nano-TiO2 particle (2 wt.%, 4 wt.% and 6 wt.%) were fabricated by using a powder metallurgy technique (P/M), and the nano-TiO2 content was optimized in matrix. The microstructures, mechanical and high-temperature tribological properties of the synthesized composites were systematicall...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63918-4
更新日期:2020-04-22 00:00:00
abstract::To examine associations between cognitive function and ophthalmological parameters, the population-based Beijing Eye Study examined ophthalmologically and physically 3127 individuals (mean age: 64.2 ± 9.8 years). Using the mini-mental state examination, cognitive function was assessed as cognitive function score (CFS)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23314-5
更新日期:2018-03-19 00:00:00
abstract::Blood transfusion is fundamental in managing hematologic malignancies. We sought to evaluate the need and availability of blood products for patients with hematological malignancies at Uganda Cancer Institute. We prospectively studied the demand and supply of blood for patients with thrombocytopenia (platelet count ≤5...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59773-y
更新日期:2020-02-18 00:00:00
abstract::Overexpression of hST3Gal1 leads to hypersialylation of cell-surface glycoconjugates, a cancer-associated condition that promotes cell growth, migration and invasion. Upregulation of this enzyme in ovarian cancer is linked to cancer progression and metastasis, contributing also to chemotherapy resistance. Strategies f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54384-8
更新日期:2019-11-29 00:00:00
abstract::The recently evolved field of synthetic biology has revolutionized the way we think of biology as an "engineerable" discipline. The newly sprouted branch is constantly in need of simple, cost-effective and automatable DNA-assembly methods. We have developed a reliable DNA-assembly system, ZeBRα (Zero-Background Redα),...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39768-0
更新日期:2019-02-27 00:00:00
abstract::Neospora caninum is an apicomplexan parasite responsible for major economic losses due to abortions in cattle. Innate immune responses are crucial for host resistance against the infection, however the molecules involved in parasite recognition are still poorly understood. Nod2 is a cytosolic receptor that recognizes ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29289
更新日期:2016-07-05 00:00:00
abstract::Social immunity forms an essential part of the defence repertoire of social insects. In response to infestation by the parasitic mite Varroa destructor and its associated viruses, honey bees (Apis mellifera L.) have developed a specific behaviour (varroa-sensitive hygiene, or VSH) that helps protect the colony from th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25444
更新日期:2016-05-03 00:00:00
abstract::The prognosis of malignancies has improved in recent years, subsequent primary cancers (SPCs) have become more frequent. This study investigates the patterns of lung cancer involved multiple primary cancers. We enrolled 206,619 primary lung cancer patients and 2,071,922 patients with other primary malignancies from Su...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06763-2
更新日期:2017-07-25 00:00:00
abstract::The aim of this study was to investigate rheumatoid arthritis (RA), and systemic lupus erythematous (SLE) as risk factors for stroke. The study was analyzed by Using the Taiwan Longitudinal Health Insurance Database 2005 (LHID2005), this cohort study investigated patients with a recorded diagnosis of RA (N = 6114), an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05110
更新日期:2014-06-05 00:00:00
abstract::Carbon is the most well-known black material in the history of man. Throughout the centuries, carbon has been used as a black material for paintings, camouflage, and optics. Although, the techniques to make other black surfaces have evolved and become more sophisticated with time, carbon still remains one of the best ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25922
更新日期:2016-05-13 00:00:00
abstract::Acanthamoeba can cause visually destructive Acanthamoeba keratitis (AK) in contact lens (CL) users. The purpose of this study was to determine whether Acanthamoeba was present in the CL cases of CL wearers and to develop techniques to prevent the contaminations. To accomplish this, 512 CL case samples were collected f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69554-2
更新日期:2020-07-28 00:00:00
abstract::Predicting the occurrence of ventricular tachyarrhythmia (VTA) in advance is a matter of utmost importance for saving the lives of cardiac arrhythmia patients. Machine learning algorithms have been used to predict the occurrence of imminent VTA. In this study, we used a one-dimensional convolutional neural network (1-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63566-8
更新日期:2020-04-21 00:00:00
abstract::In this study, we analyze the role of different structural variants of proteins in the speciation processes. We separate human and mouse proteomes (taken as a reference) into three previously defined variants of disorder: ordered proteins (ORDPs), structured proteins with intrinsically disordered protein regions (IDPR...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61466-5
更新日期:2020-03-11 00:00:00
abstract::This study proposes a new method of visualizing the ambient dose rate distribution using artificial neural networks (ANNs) from airborne radiation monitoring results. The method was applied to the results of the airborne radiation monitoring which was conducted around the Fukushima Daiichi Nuclear Power Plant by an un...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81546-4
更新日期:2021-01-20 00:00:00
abstract::Allocation of limited nutrients, such as nitrogen (N) and phosphorus (P), among plant organs reflects the influences of evolutionary and ecological processes on functional traits of plants, and thus is related to functional groups and environmental conditions. In this study, we tested this hypothesis by exploring the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20099
更新日期:2016-02-05 00:00:00
abstract::Accidental introduction through ballast water and biofouling are currently the main factors responsible for spreading non-indigenous species in the marine realm. In the Southwestern Atlantic, two scleractinian corals, Tubastraea coccinea and T. tagusensis, have been introduced by opportunistic colonization in 1980 and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50442-3
更新日期:2019-09-27 00:00:00
abstract::This paper reports the presence of tear strips on the surface of a Ti-22Al-25Nb alloy sheet stretched at 960 °C. The test piece reveals a "bamboo"-shaped pattern on its surface, which severely affects the quality of the alloy. Microstructure analysis indicates that the formation mechanism of the tear strip is related ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01889-9
更新日期:2017-05-10 00:00:00
abstract::Porosity and permeability are the key factors in assessing the hydrocarbon productivity of unconventional (shale) reservoirs, which are complex in nature due to their heterogeneous mineralogy and poorly connected nano- and micro-pore systems. Experimental efforts to measure these petrophysical properties posse many li...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56885-y
更新日期:2020-01-08 00:00:00