Abstract:
Background:DegS is a periplasmic serine protease that is considered to be the initiator of the σE stress response pathway, and this protein plays an important role in the regulation of the stress response in E. coli. However, knowledge of the biological function and global regulatory network of DegS in Vibrio cholerae remains limited. In this study, we aimed to characterize the molecular functions and further investigate the regulatory network of degS in V. cholerae. Methods:A deletion mutant of degS was constructed in the V. cholerae HN375 strain. Bacterial colony morphology was observed by a plate-based growth experiment, and bacterial growth ability was observed by a growth curve experiment. High-throughput RNA sequencing (RNA-Seq) technology was used to analyze the differential transcriptomic profiles between the wild-type and degS mutant strains. Gene ontology (GO), pathway analysis and Gene-Act-network analysis were performed to explore the main functions of the differentially expressed genes. Quantitative real-time PCR (qRT-PCR) was performed to validate the reliability and accuracy of the RNA-Seq analysis. The complementation experiments were used to test the roles of degS and ropS in the small colony degS mutant phenotype. Results:When degS was deleted, the degS mutant exhibited smaller colonies on various media and slower growth than the wild-type strain. A total of 423 differentially expressed genes were identified, including 187 genes that were upregulated in the degS mutant compared to the wild-type strain and 236 genes that were relatively downregulated. GO categories and pathway analysis showed that many differentially expressed genes were associated with various cellular metabolic pathways and the cell cycle. Furthermore, Gene-Act network analysis showed that many differentially expressed genes were involved in cellular metabolic pathways and bacterial chemotaxis. The cAMP-CRP-RpoS signaling pathway and the LuxPQ signal transduction system were also affected by the degS mutant. The expression patterns of nine randomly selected differentially expressed genes were consistent between the qRT-PCR and RNA-seq results. The complementation experiments showed that the small colony degS mutant phenotype could be partially restored by complementation with the pBAD24-degS or pBAD24-rpoS plasmid. Discussion:These results suggest that the degS gene is important for normal growth of V. cholerae. Some of the differentially expressed genes were involved in various cellular metabolic processes and the cell cycle, which may be associated with bacterial growth. Several new degS-related regulatory networks were identified. In addition, our results suggested that the cAMP-CRP-RpoS signaling pathway may be involved in the small colony degS mutant phenotype. Overall, we believe that these transcriptomic data will serve as useful genetic resources for research on the functions of degS in V. cholerae.
journal_name
PeerJjournal_title
PeerJauthors
Huang J,Chen Y,Chen J,Liu C,Zhang T,Luo S,Huang M,Min Xdoi
10.7717/peerj.7959subject
Has Abstractpub_date
2019-10-23 00:00:00pages
e7959issn
2167-8359pii
7959journal_volume
7pub_type
杂志文章相关文献
PeerJ文献大全abstract::In summer 2012, we initiated a large-scale field experiment in southern Ontario, Canada, to determine whether exposure to clothianidin seed-treated canola (oil seed rape) has any adverse impacts on honey bees. Colonies were placed in clothianidin seed-treated or control canola fields during bloom, and thereafter were ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.652
更新日期:2014-10-30 00:00:00
abstract::It is deemed important to understand cetacean occurrence and distribution to comprehend their ecological roles. The geographical occurrence of species' niche can be used to better describe their potential distribution. The niche can be defined using environmental variables. Those variables are considered static and no...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.10000
更新日期:2020-10-05 00:00:00
abstract::Non-invasive measurements of glucocorticoid (GC) concentrations, including cortisol and corticosterone, serve as reliable indicators of adrenocortical activities and physiological stress loads in a variety of species. As an alternative to invasive analyses based on plasma, GC concentrations in saliva still represent s...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1590
更新日期:2016-01-18 00:00:00
abstract::Sugarcane contributes 80% of global sugar production and to bioethanol generation for the bioenergy industry. Its productivity is threatened by drought that can cause up to 60% yield loss. This study used RNA-Seq to gain a better understanding of the underlying mechanism by which drought-tolerant sugarcane copes with ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9608
更新日期:2020-07-28 00:00:00
abstract:Aim:Historically, the distribution of Sandhill Cranes included much of North America and extending in summer into northeast Russia. In recent years, observations of sandhill cranes in Asia during the non-breeding period have been frequently reported. However, the distribution and abundance of sandhill cranes during the...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7545
更新日期:2019-08-30 00:00:00
abstract:Background:Sexual development in Flammulina velutipes is controlled by two different mating type loci (HD and PR). The HD locus contains homeodomain (Hd) genes on two separate HD subloci: HD-a and HD-b. While the functionality of the HD-b sublocus has been largely confirmed, the status and content of the HD-a sublocus ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6292
更新日期:2019-02-22 00:00:00
abstract::Human hands, when compared to that of apes, have a series of adaptations to facilitate manipulation. Numerous studies have shown that Australopithecus afarensis and Au. africanus display some of these adaptations, such as a longer thumb relative to the other fingers, asymmetric heads on the second and fifth metacarpal...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1311
更新日期:2015-10-06 00:00:00
abstract::To investigate community shelter effects of two invasive decapod species, Hemigrapsus sanguineus and Carcinus maenas, in the Long Island Sound (LIS), we deployed artificial shelters in the intertidal and immediate subtidal zones. These consisted of five groups during the summer: a control, a resident H. sanguineus mal...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2265
更新日期:2016-07-27 00:00:00
abstract:Background:Yunnanolepidoids constitute either the most basal consecutive segments or the most primitive clade of antiarchs, a highly diversified jawed vertebrate group from the Silurian and Early Devonian periods. Although the general morphology of yunnanolepidoids is well established, their endocranial features remain...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.4808
更新日期:2018-05-28 00:00:00
abstract::Spatial genetic variation of river-dwelling freshwater fishes is typically affected by the historical and contemporary river landscape as well as life-history traits. Tropical river and stream landscapes have endured extended geological change, shaping the existing pattern of genetic diversity, but were not directly a...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.4487
更新日期:2018-03-07 00:00:00
abstract:Background:Lysyl oxidases (LOX) have been extensively studied in mammals, whereas properties and functions of recently found homologues in prokaryotic genomes remain enigmatic. Methods:LOX open reading frame was cloned from Haloterrigena turkmenica in an E. coli expression vector. Recombinant Haloterrigena turkmenica ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6691
更新日期:2019-04-05 00:00:00
abstract:BACKGROUND:The scholarly publication landscape is changing rapidly. We investigated whether the introduction of an institutional publications officer might help facilitate better knowledge of publication topics and related resources, and effectively support researchers to publish. METHODS:In September 2015, a purpose-...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3294
更新日期:2017-05-31 00:00:00
abstract::We documented significantly increasing trends in atmospheric loading of ammonium (NH4) and nitrate/nitrite (NO2/3) and decreasing trends in total phosphorus (P) and sulfate (SO4) to Flathead Lake, Montana, from 1985 to 2004. Atmospheric loading of NO2/3 and NH4 increased by 48 and 198% and total P and SO4 decreased by...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.841
更新日期:2015-03-19 00:00:00
abstract::Because microorganisms are sensitive to temperature, ongoing global warming is predicted to influence microbial community structure and function. We used large-scale warming experiments established at two sites near the northern and southern boundaries of US eastern deciduous forests to explore how microbial communiti...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.358
更新日期:2014-04-24 00:00:00
abstract::A cervical vertebra of the large, pelagic pterodactyloid pterosaur Pteranodon sp. from the Late Cretaceous Niobrara Formation of Kansas, USA is significant for its association with a tooth from the large lamniform shark, Cretoxyrhina mantelli. Though the tooth does not pierce the vertebral periosteum, the intimate ass...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6031
更新日期:2018-12-14 00:00:00
abstract:Background and Purpose:Oxidative stress is involved in normal and pathological functioning of skeletal muscle. Protection of myoblasts from oxidative stress may improve muscle contraction and delay aging. Here we studied the effect of R. coriaria sumac fruit extract on human myoblasts and zebrafish embryos in condition...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.4144
更新日期:2017-12-12 00:00:00
abstract:Background:Banteng (Bos javanicus d'Alton 1823) are an endangered species, highly sensitive to habitat structure and quality. In many areas, banteng were extinct and needed to be reintroduced to restore their population. Thus, understanding the responses of body condition of reintroduced banteng to their habitat was im...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9041
更新日期:2020-04-23 00:00:00
abstract::The adaptative bleaching hypothesis (ABH) states that, depending on the symbiotic flexibility of coral hosts (i.e., the ability of corals to "switch" or "shuffle" their algal symbionts), coral bleaching can lead to a change in the composition of their associated Symbiodinium community and, thus, contribute to the cora...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2856
更新日期:2017-01-05 00:00:00
abstract::There is a lack of stability in language difficulties across early childhood: most late talkers (LTs) resolve their difficulties by pre-school; and a significant number of children who were not LTs subsequently manifest language difficulties. Greater reliability in predicting individual outcomes is needed, which might...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1098
更新日期:2015-07-23 00:00:00
abstract::The recent increased integration of evolutionary theory into conservation programs has greatly improved our ability to protect endangered species. A common application of such theory links population dynamics and indices of genetic diversity, usually estimated from neutrally evolving markers. However, some studies hav...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1868
更新日期:2016-04-11 00:00:00
abstract:BACKGROUND:Staphylococci are commensals of the mucosal surface and skin of humans and animals, but have been implicated in infections such as otitis externa, pyoderma, urinary tract infections and post-surgical complications. Laboratory records provide useful information to help investigate these infections. Therefore,...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3198
更新日期:2017-04-13 00:00:00
abstract:Background:Acute respiratory distress syndrome (ARDS) is associated with significantly increased risk of death, and early risk stratification may help to choose the appropriate treatment. The study aimed to develop a neural network model by using a genetic algorithm (GA) for the prediction of mortality in patients with...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7719
更新日期:2019-09-16 00:00:00
abstract:BACKGROUND:The NDUFS4 gene encodes an 18-kD subunit of mitochondria complex I, and mutations in this gene lead to the development of a severe neurodegenerative disease called Leigh syndrome (LS) in humans. To investigate the disease phenotypes and molecular mechanisms of Leigh syndrome, the Ndufs4 knockout (KO) mouse h...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3339
更新日期:2017-05-18 00:00:00
abstract:Background:Herpesviral hematopoietic necrosis disease, caused by cyprinid herpesvirus-2 (CyHV-2), is responsible for massive mortalities in the aquaculture of goldfish, Carassius auratus. Permissive cell lines for the isolation and propagation of CyHV-2 have been established from various goldfish tissues by sacrificing...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9373
更新日期:2020-07-14 00:00:00
abstract::Divergences between agricultural management can result in different types of biological interactions between plants and microorganisms, which may affect food quality and productivity. Conventional practices are well-established in the agroindustry as very efficient and lucrative; however, the increasing demand for sus...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9152
更新日期:2020-06-02 00:00:00
abstract::We used qingbo (Spinibarbus sinensis) and Chinese crucian carp (Carassius auratus) to test whether numerical discrimination could be improved by the coexistence and possible cooperation of conspecies or heterospecies. We conducted a spontaneous shoal choice test of singletons, conspecific dyads and heterospecific dyad...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6619
更新日期:2019-03-11 00:00:00
abstract::"Higher" termites have been able to colonize all tropical and subtropical regions because of their ability to digest lignocellulose with the aid of their prokaryotic gut microbiota. Over the last decade, numerous studies based on 16S rRNA gene amplicon libraries have largely described both the taxonomy and structure o...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.8614
更新日期:2020-02-13 00:00:00
abstract::The study of material collected during routine monitoring surveys dealing with oil extraction and aquaculture in waters off Myanmar (North Andaman Sea) and in the Gulf of Thailand, respectively, allowed us to analyse the taxonomy and diversity of the polychaete genus Terebellides (Annelida). Three species were found, ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2313
更新日期:2016-08-16 00:00:00
abstract::The use of baited remote underwater video (BRUV) for examining and monitoring marine biodiversity in temperate marine environments is rapidly growing, however many aspects of their effectiveness relies on assumptions based on studies from the Southern Hemisphere. The addition of bait to underwater camera systems acts ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9744
更新日期:2020-08-27 00:00:00
abstract:BACKGROUND:Cancer research is a national and international priority, with the efficiency and effectiveness of current anti-tumor therapies being one of the major challenges with which physicians are faced. OBJECTIVE:To assess the impact of exposure to tobacco smoke, arsenic, and phthalates on cervical cancer treatment...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2448
更新日期:2016-09-08 00:00:00