Abstract:
:The sea lamprey (Petromyzon marinus) is one of few vertebrate species known to reproducibly eliminate large fractions of its genome during normal embryonic development. This germline-specific DNA is lost in the form of large fragments, including entire chromosomes, and available evidence suggests that DNA elimination acts as a permanent silencing mechanism that prevents the somatic expression of a specific subset of "germline" genes. However, reconstruction of eliminated regions has proven to be challenging due to the complexity of the lamprey karyotype. We applied an integrative approach aimed at further characterization of the large-scale structure of eliminated segments, including: (1) in silico identification of germline-enriched repeats; (2) mapping the chromosomal location of specific repetitive sequences in germline metaphases; and (3) 3D DNA/DNA-hybridization to embryonic lagging anaphases, which permitted us to both verify the specificity of elements to physically eliminated chromosomes and characterize the subcellular organization of these elements during elimination. This approach resulted in the discovery of several repetitive elements that are found exclusively on the eliminated chromosomes, which subsequently permitted the identification of 12 individual chromosomes that are programmatically eliminated during early embryogenesis. The fidelity and specificity of these highly abundant sequences, their distinctive patterning in eliminated chromosomes, and subcellular localization in elimination anaphases suggest that these sequences might contribute to the specific targeting of chromosomes for elimination or possibly in molecular interactions that mediate their decelerated poleward movement in chromosome elimination anaphases, isolation into micronuclei and eventual degradation.
journal_name
Genes (Basel)journal_title
Genesauthors
Timoshevskiy VA,Timoshevskaya NY,Smith JJdoi
10.3390/genes10100832subject
Has Abstractpub_date
2019-10-22 00:00:00issue
10issn
2073-4425pii
genes10100832journal_volume
10pub_type
杂志文章相关文献
Genes文献大全abstract::Cytochrome P450 enzymes encoded by MORE AXILLARY GROWTH1 (MAX1)-like genes produce most of the structural diversity of strigolactones during the final steps of strigolactone biosynthesis. The diverse copies of MAX1 in Oryza sativa provide a resource to investigate why plants produce such a wide range of strigolactones...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11111348
更新日期:2020-11-13 00:00:00
abstract::Limb-girdle muscular dystrophies (LGMD) are clinically and genetically heterogeneous conditions, presenting with a wide clinical spectrum, leading to progressive proximal weakness caused by loss of muscle fibers. MiR-206 is a member of myomiRNAs, a group of miRNAs with important function in skeletal muscle. Our aim is...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes12010085
更新日期:2021-01-12 00:00:00
abstract::Hypertrophic cardiomyopathy associated with damaging variants in the ALPK3 gene is a fairly recent discovery, and only a small number of patients have been described thus far. Here we present two additional patients with hypertrophic cardiomyopathy caused by biallelic variants in ALPK3. Genetic investigation was perfo...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11101201
更新日期:2020-10-15 00:00:00
abstract::Most of the prescribing and dispensing of medicines happens in primary care. Pharmacogenomics (PGx) is the study and clinical application of the role of genetic variation on drug response. Mounting evidence suggests PGx can improve the safety and/or efficacy of several medications commonly prescribed in primary care. ...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes11111337
更新日期:2020-11-12 00:00:00
abstract::As a highly nutritious crop, Tartary buckwheat (Fagopyrum tartaricum) strongly adapts and grows in adverse environments and is widely grown in Asia. However, its flour contains a large proportion of the hull that adheres to the testa layer of the groats and is difficult to be removed in industrial processing. Fortunat...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11040459
更新日期:2020-04-23 00:00:00
abstract::At each round of cell division, the DNA must be correctly duplicated and distributed between the two daughter cells to maintain genome identity. In order to achieve proper chromosome replication and segregation, sister chromatids must be recognized as such and kept together until their separation. This process of cohe...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes11080902
更新日期:2020-08-07 00:00:00
abstract::Since ancient times, the importance of physical activity (PA) and of a wholesome diet for human health has been clearly recognized. However, only recently, it has been acknowledged that PA can reverse at least some of the unwanted effects of a sedentary lifestyle, contributing to the treatment of pathologies such as h...
journal_title:Genes
pub_type: 社论
doi:10.3390/genes10121043
更新日期:2019-12-16 00:00:00
abstract::Hepatic oval cells (HOCs) are considered the progeny of the intrahepatic stem cells that are found in a small population in the liver after hepatocyte proliferation is inhibited. Due to their small number, isolation and capture of these cells constitute a challenging task for immunosensor technology. This work describ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9020089
更新日期:2018-02-14 00:00:00
abstract::B chromosomes are enigmatic heritable elements found in the genomes of numerous plant and animal species. Contrary to their broad distribution, most B chromosomes are non-essential. For this reason, they are regarded as genome parasites. In order to be stably transmitted through generations, many B chromosomes exhibit...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes10020123
更新日期:2019-02-08 00:00:00
abstract::Amazonian puffing snakes (Spilotes; Colubridae) are snakes widely distributed in the Neotropical region. However, chromosomal data are scarce in this group and, when available, are only limited to karyotype description using conventional staining. In this paper, we focused on the process of karyotype evolution and tre...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10040288
更新日期:2019-04-09 00:00:00
abstract::In this contribution, the aspects of reptile and amphibian speciation that emerged from research performed over the past decade are reviewed. First, this study assesses how patterns and processes of speciation depend on knowing the taxonomy of the group in question, and discuss how integrative taxonomy has contributed...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes10090646
更新日期:2019-08-26 00:00:00
abstract::Altered melanosome transport in melanocytes, resulting from variants in the melanophilin (MLPH) gene, are associated with inherited forms of coat color dilution in many species. In dogs, the MLPH gene corresponds to the D locus and two variants, c.-22G > A (d1) and c.705G > C (d2), leading to the dilution of coat colo...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11060639
更新日期:2020-06-10 00:00:00
abstract::Interleukin 8 (IL-8) participates in the immune response and has the function of inducing neutrophils to release lysosomal enzymes and eliminate pathogens. This study was to investigate the effect of single nucleotide mutations in the IL-8 gene promoter region on the coccidiosis resistance index. In this study, 180 in...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11050476
更新日期:2020-04-27 00:00:00
abstract::The telomerase RNA in yeasts is large, usually >1000 nt, and contains functional elements that have been extensively studied experimentally in several disparate species. Nevertheless, they are very difficult to detect by homology-based methods and so far have escaped annotation in the majority of the genomes of Saccha...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9080372
更新日期:2018-07-26 00:00:00
abstract::The presence and strength of resource competition can influence how organisms adaptively respond to environmental change. Selection may thus reflect a balance between two forces, adaptation to an environmental optimum and evolution to avoid strong competition. While this phenomenon has previously been explored in the ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11121433
更新日期:2020-11-28 00:00:00
abstract::We set out to assess the NIH/3T3 cell proliferation activity of Arabidopsis oil body-expressed recombinant oleosin-hEGF-hEGF protein. Normally, human epidermal growth factor (hEGF) is purified through complex process, however, oleosin fusion technology provides an inexpensive and scalable platform for its purification...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11090959
更新日期:2020-08-19 00:00:00
abstract::Accurate species identification from ancient DNA samples is a difficult task that would shed light on the evolutionary history of pathogenic microorganisms. The field of palaeomicrobiology has undoubtedly benefited from the advent of untargeted metagenomic approaches that use next-generation sequencing methodologies. ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9080418
更新日期:2018-08-20 00:00:00
abstract::Long non-coding (lnc) RNAs serve a multitude of functions in cells. NEAT1 RNA is a highly abundant 4 kb lncRNA in nuclei, and coincides with paraspeckles, nuclear domains that control sequestration of paraspeckle proteins. We examined NEAT1 RNA levels and its function in 3T3-L1 cells during differentiation to adipocyt...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes5041050
更新日期:2014-11-27 00:00:00
abstract::Root nodule symbioses (nodulation) and whole genome duplication (WGD, polyploidy) are both important phenomena in the legume family (Leguminosae). Recently, it has been proposed that polyploidy may have played a critical role in the origin or refinement of nodulation. However, while nodulation and polyploidy have been...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes8120357
更新日期:2017-11-30 00:00:00
abstract::Heat stress (HS) is challenging in humans and animals as it is a complicated regulatory mechanism. This prompted us to characterize the physiological and molecular responses of a HS-animal model. In this study, a rat model system was developed by using three temperature treatments (40 ℃, 42 ℃, and 43 ℃) and sixteen bi...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11030306
更新日期:2020-03-13 00:00:00
abstract::DNA methylation patterns have been shown to change throughout the normal aging process. Several studies have found epigenetic aging markers using age predictors, but these studies only focused on blood-specific or tissue-common methylation patterns. Here, we constructed nine tissue-specific age prediction models using...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10110888
更新日期:2019-11-04 00:00:00
abstract::This study investigated the effects of dietary terrestrial animal and plant proteins on the intestinal transcriptomes of yellowtail kingfish (YTK), Seriola lalandi, an ecologically and economically important marine species in Australia. Five diets containing fish meal (FM), poultry by-product meal (PBM), blood meal (B...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11060621
更新日期:2020-06-05 00:00:00
abstract::Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or short arrays of satell...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11060663
更新日期:2020-06-18 00:00:00
abstract::The conserved Notch pathway is reported to be involved in progesterone synthesis and secretion; however, the exact effects remain controversial. To determine the role and potential mechanisms of the Notch signaling pathway in progesterone biosynthesis in porcine granulosa cells (pGCs), we first used a pharmacological ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11020120
更新日期:2020-01-22 00:00:00
abstract::Alterations in the tumor suppressor phosphatase and tensin homolog (PTEN) occur in a substantial proportion of solid tumors. These events drive tumorigenesis and tumor progression. Given its central role as a downregulator of the phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway, PTEN i...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes11070719
更新日期:2020-06-28 00:00:00
abstract::Several species of herbivores feed on maize in field and storage setups, making the development of multiple insect resistance a critical breeding target. In this study, an association mapping panel of 341 tropical maize lines was evaluated in three field environments for resistance to fall armyworm (FAW), whilst bulke...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11060689
更新日期:2020-06-24 00:00:00
abstract::Leafminer insects of the genus Liriomyza are small flies whose larvae feed on the internal tissue of some of the most important crop plants for the human diet. Several of these pest species are highly uniform from the morphological point of view, meaning molecular data represents the only reliable taxonomic tool usefu...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9110554
更新日期:2018-11-15 00:00:00
abstract::In human pre-mRNA splicing, infrequent errors occur resulting in erroneous splice products as shown in a genome-wide approach. One characteristic subgroup consists of products lacking one cassette exon. The noise in the splicing process, represented by those misspliced products, can be increased by cold shock treatmen...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes2030562
更新日期:2011-08-02 00:00:00
abstract::Gene doping, an activity which abuses and misuses gene therapy, is a major concern in sports and horseracing industries. Effective methods capable of detecting and monitoring gene doping are urgently needed. Although several PCR-based methods that detect transgenes have been developed, many of them focus only on a sin...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11040457
更新日期:2020-04-23 00:00:00
abstract::The etiology and reasons underlying the ethnic disparities in systemic sclerosis (SSc) remain unknown. African Americans are disproportionally affected by SSc and yet are underrepresented in research. The aim of this study was to comprehensively investigate the association of DNA methylation levels with SSc in dermal ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes12020129
更新日期:2021-01-20 00:00:00