Abstract：
:We present a new model of ESR1 network regulation based on analysis of Doxorubicin, Estradiol, and TNFα combination treatment in MCF-7. We used Doxorubicin as a therapeutic agent, TNFα as marker and mediator of an inflammatory microenvironment and 17β-Estradiol (E2) as an agonist of Estrogen Receptors, known predisposing factor for hormone-driven breast cancer, whose pharmacological inhibition reduces the risk of breast cancer recurrence. Based on the results of transcriptomics analysis, we found 71 differentially expressed genes that are specific for the combination treatment with Doxorubicin + Estradiol + TNFα in comparison with single or double treatments. The responsiveness to the triple treatment was examined for seven genes by qPCR, of which six were validated, and then extended to four additional cell lines differing for p53 and/or ER status. The results of differential regulation enrichment analysis highlight the role of the ESR1 network that included 36 of 71 specific differentially expressed genes. We propose that the combined activation of p53 and NF-kB transcription factors significantly influences ligand-dependent, ER-driven transcriptional responses, also of the ESR1 gene itself. These results provide a model of coordinated interaction of TFs to explain the Doxorubicin, E2 and TNFα induced repression mechanisms.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Nassiri I,Inga A,Meškytė EM,Alessandrini F,Ciribilli Y,Priami Cdoi
10.1038/s41598-019-51349-9subject
Has Abstractpub_date
2019-10-23 00:00:00pages
15172issue
1issn
2045-2322pii
10.1038/s41598-019-51349-9journal_volume
9pub_type
杂志文章abstract：:The promising ability of a plasma wiggler based on laser wakefield acceleration to produce betatron X-rays with photon energies of a few keV to hundreds of keV and a peak brilliance of 10(22)-10(23) photons/s/mm(2)/mrad(2)/0.1%BW has been demonstrated, providing an alternative to large-scale synchrotron light sources....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30491
更新日期：2016-07-26 00:00:00
abstract：:Amblyopia is a common visual disorder that is treatable in childhood. However, therapies have limited efficacy in adult patients with amblyopia. Fluoxetine can reinstate early-life critical period-like neuronal plasticity and has been used to recover functional vision in adult rats with amblyopia. We conducted a Phase...
journal_title：Scientific reports
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/s41598-018-31169-z
更新日期：2018-08-27 00:00:00
abstract：:Primary human hepatocytes are necessary to evaluate cytotoxicity, drug metabolism, and drug-drug interactions for candidate compounds in early-phase drug discovery and development. However, these analyses are often hampered by limited resources and functional or genetic variation among lots. HepaRG human hepatocellula...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03146-5
更新日期：2017-06-06 00:00:00
abstract：:Conventional concentrators with inhomogeneous coating materials that fully enclose the destined region pose great challenges for fabrication. In this paper, we propose to design an EM concentrator with homogeneous materials. Distinguished from conventional ones, the elaborately designed EM concentrator features a conc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28050-4
更新日期：2018-06-25 00:00:00
abstract：:Bacterial NhaB Na+/H+ exchangers belonging to the Ion Transporter superfamily are poorly characterized in contrast to Na+/H+ exchangers of the Cation Proton Antiporter superfamily which have NhaA from Escherichia coli as a prominent member. For a more detailed understanding of the intricacies of the exchanger's transp...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-10-28 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章,meta分析
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2013-01-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-03-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2015-11-04 00:00:00
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journal_title：Scientific reports
pub_type： 临床试验,杂志文章
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更新日期：2016-10-31 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51475-4
更新日期：2019-10-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33477-w
更新日期：2018-10-11 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01067
更新日期：2013-01-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50823-8
更新日期：2019-10-08 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80878-x
更新日期：2021-01-12 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13228
更新日期：2015-08-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-020-63914-8
更新日期：2020-04-24 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43646-0
更新日期：2019-05-08 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56407-w
更新日期：2019-12-27 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45515-2
更新日期：2019-06-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00603-z
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79882-y
更新日期：2020-12-31 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23500-5
更新日期：2018-03-26 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章,多中心研究
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更新日期：2020-05-12 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01103
更新日期：2013-01-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34642
更新日期：2016-10-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07927
更新日期：2015-01-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62016-9
更新日期：2020-03-31 00:00:00
abstract：:Results from live cell imaging of fluorescently tagged Cellulose Synthase (CESA) proteins in Cellulose Synthesis Complexes (CSCs) have enhanced our understanding of cellulose biosynthesis, including the mechanisms of action of cellulose synthesis inhibitors. However, this method has been applied only in Arabidopsis th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18994-4
更新日期：2018-01-15 00:00:00
