Abstract:
:A previous study by our group suggested that testis expressed 10 (Tex10) contributes to tumor progression by promoting stem cell‑like features in hepatocellular carcinoma. However, the relevance of pluripotency factor Tex10 in esophageal squamous cell carcinoma (ESCC) has remained elusive. The objective of the present study was to investigate the role of Tex10 in ESCC. For this purpose, the mRNA and protein expression of Tex10 was detected by reverse transcription‑quantitative PCR, western blot analysis and immunohistochemistry. In a loss‑of‑function experiment, EC109 cells were transfected with lentiviral vectors containing Tex10 short hairpin RNA or negative control. Cell proliferation was assessed using a Cell Counting kit‑8, and flow cytometry was used to analyze apoptosis and the cell cycle. Transwell assays were employed to examine the migratory and invasive capacity, and a sphere formation assay was performed to assess the clonogenicity of the EC109 cells. The results revealed that the elevated expression of Tex10 was positively associated with malignancy and with epithelial‑mesenchymal transition (EMT)‑associated mesenchymal markers in human ESCC specimens. The knockdown of Tex10 led to the inhibition of cell proliferation, the induction of apoptosis and cell cycle arrest, and decreased the stemness, migratory and invasive capacity of the EC109 cells. Furthermore, the silencing of Tex10 enhanced the sensitivity of the ESCC cells to 5‑fluorouracil. In addition, the present study revealed that Tex10 plays an essential role in regulating EMT via the activation of Wnt/β‑catenin signaling. On the whole, the findings of the present study suggest that the downregulation of Tex10 in ESCC specimens is significantly associated with tumor malignancy, and that Tex10 promotes stem cell‑like features and induces the EMT of ESCC cells through the enhancement of Wnt/β‑catenin signaling.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Xiang X,Xiong R,Yu C,Deng L,Bie J,Xiao D,Chen Z,Zhou Y,Li X,Liu K,Feng Gdoi
10.3892/or.2019.7376subject
Has Abstractpub_date
2019-12-01 00:00:00pages
2600-2610issue
6eissn
1021-335Xissn
1791-2431journal_volume
42pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Aberrant methylation of 5' CpG islands is thought to play an important role in the inactivation of tumor suppressor genes in several types of cancers. In non-small cell lung cancer (NSCLC), several genes are known to be frequently methylated and the correlation of their methylation with clinical features has been stud...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-07-01 00:00:00
abstract::Human leukocyte antigen B (HLA-B), a novel member of the NF-kappaB signal pathway in laryngeal squamous cell carcinoma (LSCC), mediates immunological surveillance of tumor cells by presenting peptides to cytotoxic T-lymphocytes (CTLs) together with S100 calcium binding protein A8 (S100A8). The objective of this study ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-06-01 00:00:00
abstract::The present study evaluated activity and toxicity of modulated doses of gemcitabine associated to oxaliplatin in patients with secondary CIRS and with locally advanced pancreatic adenocarcinoma (LAPC) and metastatic pancreatic adenocarcinoma (MPC). Since January 2006, untreated LAPC and MPC patients have been assessed...
journal_title:Oncology reports
pub_type: 临床试验,杂志文章
doi:10.3892/or.2011.1517
更新日期:2012-02-01 00:00:00
abstract::The overexpression of EGFR and/or HER-2 is associated with tumor cell resistance to chemotherapy, radiotherapy, disease progression and poor prognosis in patients with a variety of malignant tumors. Treatment combining the EGFR-targeting drug, gefitinib (ZD1839, Iressa) with the HER-2-targeting drug, trastuzumab (Herc...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-08-01 00:00:00
abstract::B7-H1 is a co-inhibitory molecule belonging to the B7 family. The B7-H1 protein is only expressed on macrophage lineage of cells in normal tissues, but is overexpressed in most types of tumor. The aberrant expression of cell surface B7-H1 on cancer cells is generally associated with high-risk prognostic factors. The t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2686
更新日期:2013-11-01 00:00:00
abstract::Dysregulation of microRNAs (miRNAs) occurs frequently in cervical carcinogenesis. miRNAs function as tumor-suppressors or oncogenes and are involved in tumor behavior. However, the expression and function of miR-27b in cervical carcinogenesis remain unknown. In the present study, we observed that miR-27b was significa...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4500
更新日期:2016-03-01 00:00:00
abstract::Telomerase is a ribonucleoprotein enzyme which is endogenously expressed in germ, stem and tumor cells, but absent in benign somatic cells. The two major telomerase components are human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT). It has been shown that hTERT is rate-limiting for telomerase...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::Rad52 encodes a protein which is required for recombinational repair of double-strand breaks. It is also associated with breast cancer predisposition genes BRCA1 and BRCA2. Mutations in the genes Rad51 or Rad52 result in severe defects in genetic recombination and the repair of double-strand DNA breaks. In order to ex...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::We performed methylation specific PCR to explore the mechanism of inactivation of tumor suppressor genes P15, P16, P53 and VHL in 48 oral SCC. The frequencies of aberrant methylation on the promoter of the P15, the P16, the P53 and the VHL genes were 0.27 (13/48), 0.42 (20/48), 0.04 (2/48) and none, respectively. Alto...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::Insulin resistance (IR) is reportedly involved in the progression of hepatocellular carcinoma (HCC). Since neovascularization plays an important role in hepatocarcinogenesis and IR, an angiostatic therapy may be considered as one of the promising approaches for chemoprevention again...
journal_title:Oncology reports
pub_type: 杂志文章,随机对照试验
doi:10.3892/or.2011.1433
更新日期:2011-12-01 00:00:00
abstract::Exhausted cluster of differentiation (CD)8+ T cells lose immunological activity due to mitochondrial dysfunction caused by peroxisome proliferator‑activated receptor γ coactivator 1α (PGC‑1α) inactivation, resulting in a poor prognosis in patients with cancer. As hydrogen gas was recently reported to activate PGC‑1α, ...
journal_title:Oncology reports
pub_type: 杂志文章,随机对照试验
doi:10.3892/or.2018.6841
更新日期:2019-01-01 00:00:00
abstract::The tumour suppressor protein p53 (wild-type = wt-p53) is of major importance in the genetic integrity of the cell. Mutations of the p53-gene (mt-p53) are the most frequent genetic aberrations identified in different tumour entities. As analyzed in a wide variety of human malignomas, mt-p53 evokes a specific immune re...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000568
更新日期:2009-12-01 00:00:00
abstract::Chromosomal translocations are very common in human cancer. The molecular mechanisms of chromosomal translocations are complex and are not fully understood. Recent studies showed organization of genomes is higher-order in the nucleus and every chromosome or chromatin has its preferential position and territory. These ...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2013.2677
更新日期:2013-11-01 00:00:00
abstract::The tumor microenvironment plays an important role in cancer growth, invasion and metastasis. The stroma surrounding a tumor is known to contain a variety of factors that can increase angiogenesis, cancer growth and tumor progression. The aim of the present study was to determine the role of fascin in cancer growth an...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6847
更新日期:2019-01-01 00:00:00
abstract::Dendritic cell-mediated cancer immunotherapy employs several ways to engage tumor antigens. We have demonstrated in both pre-clinical animal studies and early clinical trials that dendritomas, highly purified hybrids between dendritic cells (DC) and tumor cells, are superior activators of anti-tumor immunity. It has b...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::Somatic inactivation of the von Hippel-Lindau (VHL) gene is the most frequent genetic event observed in clear cell renal cell carcinoma (CC-RCC). However, the prognostic relevance of somatic VHL alteration and its target, hypoxia inducible factor (HIF)-1alpha has not been defined. We investigated the genetic changes i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract::The aim of the present study was to investigate the efficacy of using human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) as gene delivery vectors in the treatment of ovarian cancer. Lentivectors overexpressing cytosine deaminase (CD) and herpes simplex virus thymidine kinase (HSV-tk) (pGC-FU-CD-TK) ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2898
更新日期:2014-02-01 00:00:00
abstract::After having carefully checked the original data of Fig. 3, the authors noted that the student in their research group had inadvertently selected incorrect images for the 10 and 20 µM lycorine experiments to show the effect of lycorine on the migration of HepG2 cells during the figure compilation process. The correcte...
journal_title:Oncology reports
pub_type: 杂志文章,已发布勘误
doi:10.3892/or.2019.7362
更新日期:2019-12-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is known to play a central role in tumour angiogenesis. Up to now inconclusive data have been published on the clinical-biological significance of circulating VEGF and on the most suitable blood fraction for measuring it. The aims of this pilot study were to assess VEGF in blo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-02-01 00:00:00
abstract::Treatment of patients affected by advanced or inoperable GIST was revolutionized by the use of the tyrosine kinase inhibitors. Despite the fact that most patients have a good durable response of disease, they develop a resistance to treatments after a median time of 24 months. The acquired resistance is an emerging as...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or_00000361
更新日期:2009-06-01 00:00:00
abstract::Tumor angiogenesis, growth and metastasis are three closely related processes. We therefore explored the effects of (-)-oleocanthal (OC) on the three processes in melanoma and investigated underlying mechanisms. In vitro, OC suppressed proliferation, migration, invasion and induced apoptosis in melanoma cells. In addi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5270
更新日期:2017-01-01 00:00:00
abstract::The roles and mechanisms of mini-chromosome maintenance complex component 7 (MCM7) amplification and overexpression in esophageal carcinogenesis were investigated. By analyzing the TCGA datasets, we found that MCM7 was amplified in approximately 12% of esophageal squamous cell carcinomas (ESCCs), and in more than 4% o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5614
更新日期:2017-06-01 00:00:00
abstract::The aim of the present study was to evaluate human epididymis protein 4 (HE4) as a marker of epithelial ovarian cancer (EOC) relapse and the combination of this biomarker with contrast-enhanced high-resolution multidetector row computed tomography CE CT imaging to impove the monitoring of EOC patients. Twenty-one pati...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2682
更新日期:2013-11-01 00:00:00
abstract::The anaphylatoxin C5a is a chemoattractant that can induce various inflammatory responses in vivo via the C5a receptor (C5aR). There is emerging evidence that C5a is generated in the cancer microenvironment. However, the role of C5a in human nasopharyngeal carcinoma (NPC) remains largely unclear. Thus, the present stu...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3420
更新日期:2014-11-01 00:00:00
abstract::A phase I study of S-1 and biweekly docetaxel (DOC) combination therapy was conducted to determine the maximum tolerated dose (MTD) and pharmacokinetic parameters. Fourteen patients with advanced or recurrent gastric cancer were analyzed. The treatment consisted of S-1 [body surface area (BSA) <1.25 m2:80 mg/day, 1.25...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract::MA128, a novel herbal medicine, was previously identified and its effectiveness in the treatment of asthma and atopic dermatitis (AD) was demonstrated. In particular, post-inflammatory hyperpigmentation (PIH) in AD mice was improved by treatment with MA128. In addition, MA128 exhibited anti-melanogenic activity by inh...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4018
更新日期:2015-08-01 00:00:00
abstract::The present study determined the anticancer activity and its mechanism of microRNA‑133b on cell proliferation of cisplatin-induced non-small cell lung cancer cells. The expression of microRNA-133b cisplatin‑induced non-small cell lung cancer (NSCLC) tissue was lower than that of para-carcinoma tissue in patients. Over...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6215
更新日期:2018-03-01 00:00:00
abstract::Despite significant advances in micro-metastasis detection methods, little is known about the relationship between micro-metastasis and primary tumors. The purpose of this study was to assess the ability of expression of the breast cancer-related markers, HER-2/neu, COX-2, VEGF and PDGF-B, as a predictor for micro-met...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::We radiographically evaluated the response of primary lesions to chemotherapy in 101 patients with inoperable advanced gastric cancer. The median survival time in the 50 responders (49.5%) was 379 days, as compared with only 210 days in the 51 non-responders (50.1%) (p<0. 001). Morphologic changes of the gastric mucos...
journal_title:Oncology reports
pub_type: 临床试验,杂志文章
doi:10.3892/or.7.6.1279
更新日期:2000-11-01 00:00:00
abstract::In this study 10(7) peripheral blood mononuclear cells including on average 78.56% CD19+/CD5+ lymphocytes were irradiated, and then administered intradermally as an anti-cancer vaccine to seventeen patients with B-cell chronic lymphocytic leukemia (B-CLL) at early stages (twelve injections, four at a weekly interval f...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-08-01 00:00:00