Interleukin-6 and tumour necrosis factor-α cooperatively promote cell cycle regulators and proliferate rheumatoid arthritis fibroblast-like synovial cells.

Abstract:

:Objective: To elucidate the roles of interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) in cell cycle regulation and proliferation of rheumatoid arthritis fibroblast-like synovial cells (RA-FLSs). Methods: Under stimulation with IL-6/soluble interleukin-6 receptor (sIL-6R) and TNF-α, we examined the expression of cell cycle regulators [p16INK4a, p21Cip1, p27Kip1, cyclin-dependent kinase-4 (CDK4), CDK6, Cyclin D, Cyclin E, and retinoblastoma protein (pRB)] by quantitative polymerase chain reaction, Western blotting, and immunofluorescence staining. The expression of pRB, with or without 10% foetal bovine serum, was examined by Western blotting. DNA synthesis and cell viability were examined by the BrdU assay and WST-8 assay, respectively. After transfection with siRNA/p16INK4a, siRNA/p21Cip1, siRNA/p27Kip1, siRNA/CDK4, or siRNA/CDK6, RA-FLSs were successively stimulated with or without IL-6/sIL-6R or TNF-α to determine cell viability. Results: IL-6/sIL-6R significantly decreased the expression of p16INK4a, and increased p21Cip1, Cyclin E1, CYCLIN D, and pRB. TNF-α decreased the expression of CDK4, and significantly increased p27Kip1, CDK6, Cyclin E1/E2, CYCLIN D, CYCLIN E, pRB, and phosphorylated pRB (phospho-pRB). By immunofluorescence staining, CYCLIN D and phospho-pRB were simultaneously stained in the single cell. In serum-free culture, the expression of pRB was apparently decreased. DNA synthesis and cell viability were significantly increased by IL-6/sIL-6R and TNF-α. Silencing of CDK6 attenuated the cell viability induced by IL-6 and TNF-α. Conclusion: The results indicate that IL-6 and TNF-α interact with each other in regulating the cell cycle and accelerate the proliferation of RA-FLSs.

journal_name

Scand J Rheumatol

authors

Kaneshiro K,Sakai Y,Suzuki K,Uchida K,Tateishi K,Terashima Y,Kawasaki Y,Shibanuma N,Yoshida K,Hashiramoto A

doi

10.1080/03009742.2019.1602164

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

353-361

issue

5

eissn

0300-9742

issn

1502-7732

journal_volume

48

pub_type

杂志文章
  • Monitoring total-body inflammation and damage in joints and entheses: the first follow-up study of whole-body magnetic resonance imaging in rheumatoid arthritis.

    abstract:OBJECTIVE:To investigate changes in whole-body magnetic resonance imaging (WBMRI) inflammatory and structural lesions in most joints and entheses in patients with rheumatoid arthritis (RA) treated with adalimumab. METHODS:WBMRI was obtained at weeks 0, 6, 16, and 52 in a 52 week follow-up study of 37 RA patients start...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.1080/03009742.2016.1231338

    authors: Axelsen MB,Eshed I,Østergaard M,Hetland ML,Møller JM,Jensen DV,Krintel SB,Hansen MS,Terslev L,Klarlund M,Poggenborg RP,Balding L,Pedersen SJ

    更新日期:2017-07-01 00:00:00

  • Clinical outcome after one year following samarium-153 particulate hydroxyapatite radiation synovectomy.

    abstract::The clinical outcome and tolerability following treatment with samarium-153 particulate hydroxyapatite was evaluated in patients with persistent rheumatoid knee synovitis. The clinical review of 18 patients treated with intra-articular samarium-153 particulate hydroxyapatite combined with triamcinolone hexacetonide wh...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009749609065647

    authors: Clunie G,Lui D,Cullum I,Ell PJ,Edwards JC

    更新日期:1996-01-01 00:00:00

  • IgG and IgA subclass distribution of total immunoglobulin and rheumatoid factors in rheumatoid tissue plasma cells.

    abstract::The subclass distribution of IgG and IgA plasma cells, and in IgG and IgA rheumatoid factor (RF) producing cells was studied in sections of synovial tissue from seropositive RA and various types of seronegative arthritis, including ankylosing spondylitis, psoriatic arthritis, and Reiter's syndrome. The study was perfo...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009749009096788

    authors: Mellbye OJ,Vartdal F,Pahle J,Mollnes TE

    更新日期:1990-01-01 00:00:00

  • Collagenase synthesis of rheumatoid arthritis synoviocytes: dose-dependent stimulation by substance P and capsaicin.

    abstract::The synthesis and release of collagenase in the presence of the neuropeptide substance P (SP) and capsaicin, were investigated in vitro using identical synoviocyte cultures from patients with rheumatoid arthritis (RA). On average 10(-12) M SP augmented statistically significantly the collagenase production by approxim...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009749109165283

    authors: Partsch G,Matucci-Cerinic M,Marabini S,Jantsch S,Pignone A,Cagnoni M

    更新日期:1991-01-01 00:00:00

  • Influence of auranofin on lymphocyte membrane fluidity in rheumatoid arthritis. A fluorescence polarization study.

    abstract::Using the fluorescence polarization of 1.6-diphenyl-1, 3, 5-hexatriene, the membrane fluidity of lymphocytes obtained from rheumatoid arthritis patients was measured during 12 months of auranofin treatment. Significant differences in fluorescence polarization values were observed following auranofin treatment. The cor...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009748909102104

    authors: Beccerica E,Piergiacomi G,Curatola G,Ferretti G

    更新日期:1989-01-01 00:00:00

  • Pulmonary miliary tuberculosis in a patient with anti-TNF-alpha treatment.

    abstract::No difference in the number of serious adverse events was reported in previous clinical trials in rheumatoid arthritis (RA) and inflammatory bowel disease (IBD) patients treated with TNF-alpha blockade, but a patient died because of disseminated tuberculosis. A tuberculosis reactivation in a patient with IBD and anti-...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.1080/030097402317255372

    authors: Mayordomo L,Marenco JL,Gomez-Mateos J,Rejon E

    更新日期:2002-01-01 00:00:00

  • Significance of C-reactive protein elevation in the pretreatment stage of systemic lupus erythematosus.

    abstract::The significance of C-reactive protein (CRP) elevation in patients with systemic lupus erythematosus (SLE) in the pretreatment stage was assessed with reference to other laboratory data obtained simultaneously. Sixteen of 31 cases were CRP-positive, and the CR-positive group contained significantly more cases with uri...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009748109095302

    authors: Inoue T,Kanayama Y,Katoh N,Ohe A,Amatsu K,Okamura M,Horiguchi T

    更新日期:1981-01-01 00:00:00

  • In situ characterization of the cellular infiltrate in labial and palatine glands in Sjögren's syndrome.

    abstract::The inflammatory cells in the minor salivary glands in situ in 11 patients with Sjögren's syndrome were characterized by using intracellular markers for T lymphocytes, plasma cells, mononuclear phagocytes and granulocytes. The methods used to demonstrate the markers were optimized histochemical and immunoperoxidase te...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009748109095324

    authors: Konttinen YT

    更新日期:1981-01-01 00:00:00

  • Intra-articular glucocorticoid treatment of the rheumatoid wrist. An ultrasonographic study.

    abstract::Fifty consecutive patients with RA and clinical wrist synovitis were randomly allocated to either receive an injection of glucocorticoid into the radiocarpal joint or have the same amount of drug divided into the radiocarpal and midcarpal joints. Inferior radioulnar joint synovitis was treated with extra steroid injec...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 临床试验,杂志文章

    doi:10.1080/030097401753180336

    authors: Koski JM,Hermunen H

    更新日期:2001-01-01 00:00:00

  • Oral contraceptives in systemic lupus erythematosus: side-effects and influence on the activity of SLE.

    abstract::The risk of a disease flare-up and the side-effects experienced during the use of oral contraceptives (OCs) were studied in 85 female SLE patients, 18-44 years old, regularly attending two specialist rheumatological clinics. Thirty-one patients had used combined oral contraceptives (cOCs) during or after the onset of ...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009749109096822

    authors: Julkunen HA

    更新日期:1991-01-01 00:00:00

  • Prevalence and predictive factors of comorbidity in rheumatoid arthritis patients monitored prospectively from disease onset up to 20 years: lack of association between inflammation and cardiovascular disease.

    abstract:OBJECTIVES:To study the prevalence of comorbid conditions at diagnosis and during follow-up in a cohort of patients with early rheumatoid arthritis (RA) followed prospectively over 20 years, and to identify possible early predictive factors for future comorbidities. METHODS:A community-based cohort of 183 patients (me...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009741003674180

    authors: Kapetanovic MC,Lindqvist E,Simonsson M,Geborek P,Saxne T,Eberhardt K

    更新日期:2010-01-01 00:00:00

  • Gammalinolenic acid treatment of fatigue associated with primary Sjögren's syndrome.

    abstract:OBJECTIVE:To evaluate the efficacy of the essential omega-6 fatty acid Gammalinolenic acid (GLA) on fatigue associated with primary Sjögren's syndrome. METHODS:Ninety patients with primary Sjögren's syndrome (with or without signs of autoimmunity) entered a 6-month double blind placebo-controlled randomised trial with...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1080/03009740252937577

    authors: Theander E,Horrobin DF,Jacobsson LT,Manthorpe R

    更新日期:2002-01-01 00:00:00

  • Glycosaminoglycan polysulphate treatment in experimental osteoarthritis in rabbits.

    abstract::The effect of glycosaminoglycan polysulphate as medication for osteoarthritis was studied in an experimental model of osteoarthritis induced by periodic immobilization of rabbit knees. In a pilot experiment with 5 weeks of continuous immobilization there were indistinct differences between treated and control groups. ...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009748309098538

    authors: Vanharanta H

    更新日期:1983-01-01 00:00:00

  • Burden of rheumatoid arthritis in the Nordic region, 1990-2015: a comparative analysis using the Global Burden of Disease Study 2015.

    abstract:OBJECTIVE:To report mortality and disability due to rheumatoid arthritis (RA) in the Nordic region (Denmark, Finland, Greenland, Iceland, Norway, and Sweden) using data from the Global Burden of Disease Study (GBD) 2015. METHOD:Using the results of GBD 2015, we present rates and trends in prevalence, mortality, years ...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.1080/03009742.2017.1314002

    authors: Kiadaliri AA,Kristensen LE,Englund M

    更新日期:2018-03-01 00:00:00

  • Increased expression of the proprotein convertase enzyme FURIN in rheumatoid arthritis.

    abstract:OBJECTIVE:FURIN is a proprotein convertase enzyme that inhibits the proinflammatory function of T cells and myeloid cells. Elevated FURIN expression levels have been reported in immune-mediated diseases, such as primary Sjögren's syndrome. Here, we investigated the levels of FURIN in peripheral blood (PB) and synovial ...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.1080/03009742.2018.1520294

    authors: Valli A,Ranta N,Grönholm A,Silvennoinen O,Pesu M,Isomäki P

    更新日期:2019-05-01 00:00:00

  • Risk factors for reported influenza and influenza-like symptoms in patients with rheumatoid arthritis.

    abstract:OBJECTIVES:To determine the prevalence and predictors of influenza and influenza-like symptoms in patients with rheumatoid arthritis (RA). METHOD:Questionnaires were sent to patients registered as having RA and they were asked to fill in per month any period and details of influenza-like symptoms and vaccination. An e...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009742.2012.670729

    authors: Dirven L,Huizinga TW,Allaart CF

    更新日期:2012-10-01 00:00:00

  • Renal function is mostly preserved in patients with systemic sclerosis.

    abstract:OBJECTIVE:Chronic renal disease other than scleroderma renal crisis (SRC) is not well documented in systemic sclerosis (SSc). We examined the occurrence of decreased glomerular filtration rate (GFR) in a large consecutive SSc cohort and analysed whether it was related to SSc or could be related to other causes. METHOD...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.1080/03009740802629424

    authors: Scheja A,Bartosik I,Wuttge DM,Hesselstrand R

    更新日期:2009-01-01 00:00:00

  • Immunoprecipitates used as a reagent for rheumatoid factors.

    abstract::IgM and IgG rheumatoid factors (RFs) were purified by affinity chromatography and gel filtration. The preparations thus obtained were used as standards in a radio-immunoassay for RF detection. In this assay, RFs were reacted with immunoprecipitates, and the RFs were detected with radiolabelled (F(ab')2 fragments speci...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009748409100395

    authors: Nordfang O,Høier-Madsen M,Danielsen A

    更新日期:1984-01-01 00:00:00

  • Lysozyme concentrations in synovial fluid, pleural fluid and thoracic duct lymph in rheumatoid arthritis.

    abstract::Lysozyme (LZM) concentrations in synovial fluid were determined in patients with seropositive and seronegative rheumatoid arthritis (RA) and in patients whose arthritic exudates had been caused by Reiter's disease, a Yersinia enterocolitica infection, osteoarthritis, or trauma. Patients with rheumatoid disease had sig...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009748509102021

    authors: Klockars M,Pettersson T

    更新日期:1985-01-01 00:00:00

  • Elevated IL-6 levels in the synovial fluid of osteoarthritis patients stem from plasma cells.

    abstract:OBJECTIVE:To analyse the levels of interleukin-6 (IL-6) in the synovial fluids and sera of patients with osteoarthritis (OA) and to identify the IL-6-secreting cells. METHODS:Serum, synovial fluid, synovial tissue, and articular cartilage samples were collected from 49 OA patients with end-stage knee or hip OA who und...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.1080/03009740701250785

    authors: Doss F,Menard J,Hauschild M,Kreutzer HJ,Mittlmeier T,Müller-Steinhardt M,Müller B

    更新日期:2007-03-01 00:00:00

  • Antinuclear antibody screening in this new millennium: farewell to the microscope?

    abstract::ANA testing by immunofluorescence technique (F-ANA) is nowadays still performed in much the same way as 45 years ago when the test was introduced. Due to its low specificity the F-ANA test has a poor predictive value for systemic autoimmune diseases and in addition has proven difficult to standardise. In the meantime,...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.1080/030097401300162860

    authors: Nossent H,Rekvig OP

    更新日期:2001-01-01 00:00:00

  • Relative bone mineral density measured by metacarpal index (MCI) and chronic spinal syndromes: an epidemiological study.

    abstract:OBJECTIVES:The results of previous studies on the association between bone mineral density (BMD) and chronic spinal syndromes have been contradictory. Therefore, we studied relative BMD measured by the metacarpal index (MCI) and its associations with chronic neck and low-back syndromes and diffuse idiopathic skeletal h...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.1080/03009740701406536

    authors: Haara MM,Arokoski JP,Kröger H,Kärkkäinen A,Manninen P,Knekt P,Impivaara O,Heliövaara M

    更新日期:2007-11-01 00:00:00

  • Evaluation of choroidal substructure perfusion in patients affected by systemic sclerosis: an optical coherence tomography angiography study.

    abstract::Objective: Vasculopathy in systemic sclerosis (SSc) is characterized by the obliteration of arterioles and a reduced capillary density in various tissues. In SSc, atrophic alterations of the choroid have been suggested based on morphological data acquired by optical coherence tomography (OCT). In this study, we aimed ...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.1080/03009742.2019.1641616

    authors: Ranjbar M,Rothe M,Klapa S,Lange T,Prasuhn M,Grisanti S,Riemekasten G,Humrich JY

    更新日期:2020-03-01 00:00:00

  • The prevalence and severity of rheumatoid arthritis in Oslo. Results from a county register and a population survey.

    abstract::The objective was (1) to examine the prevalence of rheumatoid arthritis (RA) by a county patient register, (2) to cross-validate the register findings by a postal population survey, and (3) to estimate prevalences of disease subsets according to age, sex, and levels of physical disability. The study was performed with...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009749709065712

    authors: Kvien TK,Glennås A,Knudsrød OG,Smedstad LM,Mowinckel P,Førre O

    更新日期:1997-01-01 00:00:00

  • Differences associated with age at onset in early systemic sclerosis patients: a report from the EULAR Scleroderma Trials and Research Group (EUSTAR) database.

    abstract:OBJECTIVE:The aim of this study was to analyse differences in clinical presentation in patients with early (< 3 years' duration) systemic sclerosis (SSc), comparing three age groups according to disease subsets. METHOD:Cross-sectional analysis of the prospective EULAR Scleroderma Trials and Research database (EUSTAR) ...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章,多中心研究

    doi:10.1080/03009742.2018.1459830

    authors: Carreira PE,Carmona L,Joven BE,Loza E,Andréu JL,Riemekasten G,Vettori S,Balbir-Gurman A,Airò P,Walker U,Damjanov N,Matucci-Cerinic M,Ananieva LP,Rednic S,Czirják L,Distler O,Farge D,Hesselstrand R,Corrado A,Caramasc

    更新日期:2019-01-01 00:00:00

  • Faecal blood loss during administration of acetylsalicylic acid, ketoprofen and two new ketoprofen sustained-release compounds.

    abstract::The influence of one week's treatment with acetylsalicylic acid, ketoprofen, ketoprofen sustained-release capsules (Biovail capsules), and ketoprofen sustained-release tablets (IBP tablet) on gastrointestinal bleeding was investigated in 41 healthy male volunteers by means of a radiochromium assay. The physiological f...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/03009748309098550

    authors: Ranløv PJ,Nielsen SP,Bärenholdt O

    更新日期:1983-01-01 00:00:00

  • The effect of daily administration of naproxen on the prothrombin complex activity in patients under long-term therapy with phenprocoumon.

    abstract::Out of a total of 150 patients attending an out-patient clinic for anticoagulant therapy, 12 had disorders which normally would indicate a prolonged use of an antirheumatic drug. To 6 of these patients, naproxen (1/4 g twice daily) was given while anticoagulant treatment with phenprocoumon was continued in an unchange...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:

    authors: Petersen PB,Husted S,Mortensen A,Andreasen F

    更新日期:1979-01-01 00:00:00

  • Audit of closed synovial biopsy in the diagnosis of inflammatory joint disease.

    abstract::In a prospective study, the clinical features and synovial histopathology of 78 patients with joint disease were compared. Nineteen patients had early rheumatoid disease, 19 chronic rheumatoid, 17 chondrocalcinosis or osteoarthritis, 10 persistent monoarthritis and 14 other miscellaneous arthropathies. After semiquant...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009748509100412

    authors: Gallagher PJ,Blake DR,Lever JV

    更新日期:1985-01-01 00:00:00

  • Adherence to cardiovascular prevention strategies in patients with rheumatoid arthritis.

    abstract:OBJECTIVES:Patients with rheumatoid arthritis (RA) have a high risk of cardiovascular disease (CVD). Recent national and international guidelines suggest strict treatment of CVD risk factors in RA. The aim of this study was to evaluate the self-reported adherence to CV prevention strategies in patients with RA. METHOD...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.3109/03009742.2015.1028997

    authors: van Breukelen-van der Stoep DF,Zijlmans J,van Zeben D,Klop B,van de Geijn GJ,van der Meulen N,Hazes M,Birnie E,Castro Cabezas M

    更新日期:2015-01-01 00:00:00

  • Low body weight and involuntary weight loss are associated with Raynaud's phenomenon in both men and women.

    abstract::Objectives: Low body weight is an easily assessable cause of Raynaud's phenomenon (RP), and is frequently overlooked by clinicians. We aim to investigate the association of low body weight (body mass index < 18.5 kg/m2), involuntary weight loss, and nutritional restrictions with the presence of RP. Method: Participant...

    journal_title:Scandinavian journal of rheumatology

    pub_type: 杂志文章

    doi:10.1080/03009742.2020.1780310

    authors: Abdulle AE,Arends S,van Goor H,Brouwer E,van Roon AM,Westra J,Herrick AL,de Leeuw K,Mulder DJ

    更新日期:2020-10-16 00:00:00