Loss of Discoidin Domain Receptor 1 Predisposes Mice to Periodontal Breakdown.

Abstract:

:The discoidin domain receptors, DDR1 and DDR2, are nonintegrin collagen receptors and tyrosine kinases. DDRs regulate cell functions, and their extracellular domains affect collagen fibrillogenesis and mineralization. Based on the collagenous nature of dentoalveolar tissues, we hypothesized that DDR1 plays an important role in dentoalveolar development and function. Radiography, micro-computed tomography (micro-CT), histology, histomorphometry, in situ hybridization (ISH), immunohistochemistry (IHC), and transmission electron microscopy (TEM) were used to analyze Ddr1 knockout (Ddr1-/-) mice and wild-type (WT) controls at 1, 2, and 9 mo, and ISH and quantitative polymerase chain reaction (qPCR) were employed to assess Ddr1/DDR1 messenger RNA expression in mouse and human tissues. Radiographic images showed normal molars but abnormal mandibular condyles, as well as alveolar bone loss in Ddr1-/- mice versus WT controls at 9 mo. Histological, histomorphometric, micro-CT, and TEM analyses indicated no differences in enamel or dentin Ddr1-/- versus WT molars. Total volumes (TVs) and bone volumes (BVs) of subchondral and ramus bone of Ddr1-/- versus WT condyles were increased and bone volume fraction (BV/TV) was reduced at 1 and 9 mo. There were no differences in alveolar bone volume at 1 mo, but at 9 mo, severe periodontal defects and significant alveolar bone loss (14%; P < 0.0001) were evident in Ddr1-/- versus WT mandibles. Histology, ISH, and IHC revealed disrupted junctional epithelium, connective tissue destruction, bacterial invasion, increased neutrophil infiltration, upregulation of cytokines including macrophage colony-stimulating factor, and 3-fold increased osteoclast numbers (P < 0.05) in Ddr1-/- versus WT periodontia at 9 mo. In normal mouse tissues, ISH and qPCR revealed Ddr1 expression in basal cell layers of the oral epithelia and in immune cells. We confirmed a similar expression pattern in human oral epithelium by ISH and qPCR. We propose that DDR1 plays an important role in periodontal homeostasis and that absence of DDR1 predisposes mice to periodontal breakdown.

journal_name

J Dent Res

authors

Chavez MB,Kolli TN,Tan MH,Zachariadou C,Wang C,Embree MC,Lira Dos Santos EJ,Nociti FH Jr,Wang Y,Tatakis DN,Agarwal G,Foster BL

doi

10.1177/0022034519881136

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

1521-1531

issue

13

eissn

0022-0345

issn

1544-0591

journal_volume

98

pub_type

杂志文章
  • Artificial Intelligence in Dentistry: Chances and Challenges.

    abstract::The term "artificial intelligence" (AI) refers to the idea of machines being capable of performing human tasks. A subdomain of AI is machine learning (ML), which "learns" intrinsic statistical patterns in data to eventually cast predictions on unseen data. Deep learning is a ML technique using multi-layer mathematical...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034520915714

    authors: Schwendicke F,Samek W,Krois J

    更新日期:2020-07-01 00:00:00

  • Enamel silicon and fluoride relationships demonstrating a surface silicon effect that facilitates fluoride uptake.

    abstract::This study indicated that a clear relationship exists between silicon and fluoride. We have also demonstrated a probable surface enamel silicon effect that increases fluoride uptake. The complex set of relationships described in the surface chemistry of calcium, tin, and zirconium with fluoride, hydroxide, phosphate, ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345760550050601

    authors: Levy JS,Koritzer RT

    更新日期:1976-09-01 00:00:00

  • Electromyographic heterogeneity in the human temporalis and masseter muscles during static biting, open/close excursions, and chewing.

    abstract::The human temporalis and masseter muscles are not activated homogeneously during static bite force tasks. In this study, we studied the possible existence of regional differences in these muscles under dynamic conditions. Electromyographic (EMG) activity was recorded by means of bipolar fine-wire electrodes. Six elect...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345950740061201

    authors: Blanksma NG,van Eijden TM

    更新日期:1995-06-01 00:00:00

  • Accuracy of Kubelka-Munk reflectance theory applied to human dentin and enamel.

    abstract::The Kubelka-Munk (K-M) theory provides a reflectance model for translucent materials placed on backings of different colors. We hypothesize that Kubelka-Munk (K-M) theoretical diffuse reflectance spectra of dentin and enamel are in good agreement with observed diffuse reflectance. The aim of this study was to measure ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345010800020901

    authors: Ragain JC Jr,Johnston WM

    更新日期:2001-02-01 00:00:00

  • Effects of various resin composite (co)monomers and extracts on two caries-associated micro-organisms in vitro.

    abstract::Previous studies have shown that extracts of various filling materials, e.g., resin composites, may influence the growth of cariogenic micro-organisms. The purpose of this study was to examine the effects of important resin composite (co)monomers (Bis-GMA, UDMA, TEGDMA, EGDMA) and extracts of two commercial dental com...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345980770010601

    authors: Hansel C,Leyhausen G,Mai UE,Geurtsen W

    更新日期:1998-01-01 00:00:00

  • Changes in the microvasculature after mechanical pressure on the hamster cheek pouch.

    abstract::The reaction of the microvasculature in the periodontal ligament to mechanical pressure is considered a very important phenomenon with respect to the biological background to orthodontic tooth movement. For clarification of the microvascular reaction to mechanical pressure, an experimental model that incorporated a ha...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345920710060701

    authors: Iida J,Inage S,Kurihara S,Miura F

    更新日期:1992-06-01 00:00:00

  • Antibodies against mycobacterial antigens in the synovial fluid of patients with temporomandibular disorders.

    abstract::In the absence of active pulmonary disease, mycobacterial infection frequently causes arthritis and can be considered to initiate autoimmune diseases such as rheumatoid arthritis. Temporomandibular disorder (TMD) is a disease in which pain and impaired mandibular movement appear to arise directly from degenerative or ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345000790100401

    authors: Adachi N,Matsumoto S,Tokuhisa M,Kobayashi K,Yamada T

    更新日期:2000-10-01 00:00:00

  • Economic costs of attrition in clinical trials.

    abstract::Conventional analyses of the cost-effectiveness of preventive regimens fail to account for the impact of attrition on reported costs. Using an economic model in which costs are allocated to fixed and variable components, and data from a recently completed clinical field trial, costs per participant are shown to vary w...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345820610050601

    authors: Doherty NJ

    更新日期:1982-05-01 00:00:00

  • Colonization of the human oral cavity by a Streptococcus mutans mutant producing increased bacteriocin.

    abstract::Streptococcus mutans strain JH1005 is a mutant that produces levels of bacteriocin activity three-fold-elevated than those produced by its parent, JH1001. A single infection regimen with JH1005 was found to result in persistent colonization of the teeth of all three adult subjects tested. This is a significant improve...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345870660060101

    authors: Hillman JD,Dzuback AL,Andrews SW

    更新日期:1987-06-01 00:00:00

  • Curing light-activated composite cement through porcelain.

    abstract::The purpose of the study was to determine the exposure times needed to harden a light-activated composite cement through various thicknesses of porcelain. A negative exponential model was developed with which the relative hardness of a composite could be predicted from the product of the exposure time and the square r...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345890680030801

    authors: Chan KC,Boyer DB

    更新日期:1989-03-01 00:00:00

  • Short-chain carboxylic acid concentration in human gingival crevicular fluid.

    abstract::Short-chain carboxylic acids (e.g., lactic acid, propionic acid, butyric acid) are metabolic by-products of bacterial metabolism which can accumulate in the gingival crevice. It is of no small consequence, therefore, that 1- to 5-mM concentrations of these acids exhibit significant biological activity, including the a...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345970760010801

    authors: Niederman R,Buyle-Bodin Y,Lu BY,Robinson P,Naleway C

    更新日期:1997-01-01 00:00:00

  • The biocompatibility of dental implant materials measured in an animal model.

    abstract::The biocompatibility of two implant materials, vitallium and poly-methyl methacrylate (PMMA), was evaluated in an animal model in which bone formation and resorption are independent of each other. Neither material accelerated resorption, but formation was inhibited 12% by vitallium and 38% by PMMA. When three PMMA pre...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345810600010801

    authors: Garcia DA,Sullivan TM,O'Neill DM

    更新日期:1981-01-01 00:00:00

  • Validation of an in vitro biofilm model of supragingival plaque.

    abstract::The study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. We describe a model of supragingival plaque containing Actinomyces naeslundii, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, and Streptococcus o...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345010800011201

    authors: Guggenheim B,Giertsen E,Schüpbach P,Shapiro S

    更新日期:2001-01-01 00:00:00

  • Application of fluorescent antibody methods in the analysis of plaque samples.

    abstract::Direct FA staining for S mutans serotypes may be performed on smears made from plaque or strain isolates and on colonies attached to agar plates of black membrane filters. Staining with single conjugates directed to S mutans serotypes (a to e) as well as with polyvalent a-, b-, d-, f-fluorescein label and c-, e-rhodam...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/002203457605500126011

    authors: Thomson LA,Little W,Hageage GJ

    更新日期:1976-01-01 00:00:00

  • Characterization of dental pulp stem cells of human tooth germs.

    abstract::In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. In this present study, we characterized hDPSCs isolated from an earlier developmental stage to evaluate the potential usage of these cells for tissue-regenerative therapy. hDPSCs isolated at the crown-completed stage showed a ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910808700716

    authors: Takeda T,Tezuka Y,Horiuchi M,Hosono K,Iida K,Hatakeyama D,Miyaki S,Kunisada T,Shibata T,Tezuka K

    更新日期:2008-07-01 00:00:00

  • CD73 Controls Extracellular Adenosine Generation in the Trigeminal Nociceptive Nerves.

    abstract::Purinergic signaling is involved in pain generation and modulation in the nociceptive sensory nervous system. Adenosine triphosphate (ATP) induces pain via activation of ionotropic P2X receptors while adenosine mediates analgesia via activation of metabotropic P1 receptors. These purinergic signaling are determined by...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034517692953

    authors: Liu X,Ma L,Zhang S,Ren Y,Dirksen RT

    更新日期:2017-06-01 00:00:00

  • Anatomic and directional variation in the mechanical properties of the mandibular condyle in pigs.

    abstract::Stereologic studies of trabecular architecture suggest that the pig mandibular condyle is strongest when loaded supero-inferiorly, and that stress is concentrated in the antero-inferior region (Teng and Herring, 1995). To test these hypotheses, we investigated the uni-axial mechanical properties of 22 pig mandibular c...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345960750110501

    authors: Teng S,Herring SW

    更新日期:1996-11-01 00:00:00

  • Formation of the tooth-bone interface.

    abstract::Not only are teeth essential for mastication, but also missing teeth are considered a social handicap due to speech and aesthetic problems, with a resulting high impact on emotional well-being. Several treatment procedures are currently available for tooth replacement with mostly inert prosthetic materials and implant...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审

    doi:10.1177/0022034509355440

    authors: Fleischmannova J,Matalova E,Sharpe PT,Misek I,Radlanski RJ

    更新日期:2010-02-01 00:00:00

  • PAR-1 and PAR-2 Expression Is Enhanced in Inflamed Odontoblast Cells.

    abstract::Protease-activated receptors (PARs) are G protein-coupled receptors, which are activated by proteolytical cleavage of the amino-terminus and act as sensors for extracellular proteases. We hypothesized that PAR-1 and PAR-2 can be modulated by inflammatory stimulus in human dental pulp cells. PAR-1 and PAR-2 gene expres...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034517719415

    authors: Alvarez MMP,Moura GE,Machado MFM,Viana GM,de Souza Costa CA,Tjäderhane L,Nader HB,Tersariol ILS,Nascimento FD

    更新日期:2017-12-01 00:00:00

  • The identification of a novel locus for mandibular prognathism in the Han Chinese population.

    abstract::Mandibular prognathism is a common dentofacial phenotype with a substantial genetic component; however, few susceptibility loci have been mapped. Ethnicity is a risk factor for mandibular prognathism, and a relatively high prevalence is observed in Asian populations. The hypothesis of this study suggested that a speci...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034510382546

    authors: Li Q,Li X,Zhang F,Chen F

    更新日期:2011-01-01 00:00:00

  • Characteristics of mandibular masticatory movement in young and elderly dentate subjects.

    abstract::Dimensions and velocity of mandibular movement are important variables in the evaluation of masticatory and speech patterns. However, little information is available concerning elderly dentates and their oral motor function and behavior. Double recordings of a chewing period, from start to swallowing, were performed i...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345900690021101

    authors: Karlsson S,Carlsson GE

    更新日期:1990-02-01 00:00:00

  • Fluoride release from CaF2 and enamel demineralization.

    abstract::The anticaries effect of professional fluoride (F) application has been attributed to calcium-fluoride-like deposits (CaF(2)) formed on enamel, but this has not been clearly demonstrated. We hypothesized that CaF(2) formed on plaque-free enamel by F application would reduce enamel demineralization due to the increase ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910808701105

    authors: Tenuta LM,Cerezetti RV,Del Bel Cury AA,Tabchoury CP,Cury JA

    更新日期:2008-11-01 00:00:00

  • Formation of differentiated tissues in vivo by periodontal cell populations cultured in vitro.

    abstract::The periodontium contains heterogeneous mesenchymal cell populations with various differentiation potentials. The capacity of these cells for tissue formation as well as the origin of their precursors are still not entirely defined. In this study, cells originating from different periodontal tissues were cultured in v...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345950740051201

    authors: Lang H,Schüler N,Arnhold S,Nolden R,Mertens T

    更新日期:1995-05-01 00:00:00

  • Kinetics of cell proliferation and migration associated with orthodontically-induced osteogenesis.

    abstract::Cell kinetics of rat molar periodontal ligament (PDL) during orthodontically-induced osteogenesis revealed: 1) primary growth fraction of 59%, 2) proliferating cells from throughout the PDL migrate toward the bone surface, 3) preosteoblasts, capable of forming osteoblasts without synthesizing DNA, are present in the P...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345810600021501

    authors: Roberts WE,Chase DC

    更新日期:1981-02-01 00:00:00

  • Dissolution of mercury from amalgam into saline solution.

    abstract::Dissolution of mercury from amalgams and some intermetallic compounds found in the amalgam structure, such as gamma 1, gamma 2, and beta 1, was examined during one week of aging in 0.9% NaCl solution at 37 degrees C. The amount of mercury released from gamma 1 (30.2 micrograms/cm2) was at least 13 times that released ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345870660010701

    authors: Okabe T,Ferracane J,Cooper C,Matsumoto H,Wagner M

    更新日期:1987-01-01 00:00:00

  • Electric-current-assisted application of self-etch adhesives to dentin.

    abstract::The use of electric current during the application of etch-and-rinse adhesive systems has been recently claimed to increase bonding of etch-and-rinse adhesives by enhancing substrate impregnation. The null hypothesis tested in this study was that electrically assisted application has no effect on bond strength of self...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910608501205

    authors: Breschi L,Mazzoni A,Pashley DH,Pasquantonio G,Ruggeri A,Suppa P,Mazzotti G,Di Lenarda R,Tay FR

    更新日期:2006-12-01 00:00:00

  • Clinical, radiographic, and genetic evaluation of a novel form of autosomal-dominant oligodontia.

    abstract::A frameshift mutation recently identified within the paired domain of the transcription factor, PAX9, has been linked to a unique form of oligodontia in a single, multigenerational family (Stockton et al., 2000). We now describe the phenotypic and segregation analyses of this remarkable kindred, the initial approach t...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345000790070701

    authors: Goldenberg M,Das P,Messersmith M,Stockton DW,Patel PI,D'Souza RN

    更新日期:2000-07-01 00:00:00

  • Influence of gastric acidity on fluoride absorption in rats.

    abstract::The rate of F absorption from the stomach is pH-dependent, with greater absorption at low pH. Since the rate of absorption is also strongly influenced by the rapidity of gastric emptying, we have compared the relative importance of gastric acidity and gastric emptying in overall F absorption. Male rats (350 g, n = 85)...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345930720031101

    authors: Messer HH,Ophaug RH

    更新日期:1993-03-01 00:00:00

  • Stem Cell-Soluble Signals Enhance Multilumen Formation in SMG Cell Clusters.

    abstract::Saliva plays a major role in maintaining oral health. Patients with salivary hypofunction exhibit difficulty in chewing and swallowing foods, tooth decay, periodontal disease, and microbial infections. At this time, treatments for hyposalivation are limited to medications (e.g., muscarinic receptor agonists: pilocarpi...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034515600157

    authors: Maruyama CL,Leigh NJ,Nelson JW,McCall AD,Mellas RE,Lei P,Andreadis ST,Baker OJ

    更新日期:2015-11-01 00:00:00

  • The effects of smoking on dental care utilization and its costs in Japan.

    abstract::Smoking has been established as an important risk factor for periodontal disease and tooth loss. The purpose of this study was a prospective evaluation of the effects of smoking on dental care utilization and its costs, based on data from 5712 males aged 20-59 yrs. Age, dental health behavior, and history of diabetes ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034508327523

    authors: Ide R,Hoshuyama T,Wilson D,Takahashi K,Higashi T

    更新日期:2009-01-01 00:00:00