Abstract:
:Solid-phase synthesis is the method of choice for peptide preparation in both research and industrial settings. The whole synthetic process is governed by the initial functionalization of the resin. Although the literature provides several methods to determine such functionalization, the addition of an Fmoc-amino acid and the posterior spectrophotometric measurement of the dibenzofulvene adduct formed after Fmoc removal is the most widely used for this purpose. However, a range of molar extinction coefficient (ε) values and even wavelengths are currently used in the field, with no standardization of the method. Here, we propose a single-point standardization method that involves a standard solution of the corresponding amino acid to be checked that is prepared freshly at the time of the analysis.
journal_name
ACS Comb Scijournal_title
ACS combinatorial scienceauthors
Al Musaimi O,Basso A,de la Torre BG,Albericio Fdoi
10.1021/acscombsci.9b00154subject
Has Abstractpub_date
2019-11-11 00:00:00pages
717-721issue
11eissn
2156-8952issn
2156-8944journal_volume
21pub_type
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