Abstract:
:The endoplasmic reticulum (ER) is a multi functional organelle and plays a crucial role in protein folding and lipid biosynthesis. The SEC59 gene encodes dolichol kinase, required for protein glycosylation in the ER. The mutation of sec59-1 caused a protein N-glycosylation defect mediated ER stress resulting in increased levels of phospholipid, neutral lipid and sterol, whereas growth was reduced. In the sec59-1∆ cell, the N-glycosylation of vacuolar carboxy peptidase-Y (CPY) was significantly reduced; whereas the ER stress marker Kar2p and unfolded protein response (UPR) were significantly increased. Increased levels of Triacylglycerol (TAG), sterol ester (SE), and lipid droplets (LD) could be attributed to up-regulation of DPP1, LRO1, and ARE2 in the sec 59-1∆ cell. Also, the diacylglycerol (DAG), sterol (STE), and free fatty acids (FFA) levels were significantly increased, whereas the genes involved in peroxisome biogenesis and Pex3-EGFP levels were reduced when compared to the wild-type. The microarray data also revealed increased expression of genes involved in phospholipid, TAG, fatty acid, sterol synthesis, and phospholipid transport resulting in dysregulation of lipid homeostasis in the sec59-1∆ cell. We conclude that SEC59 dependent N-glycosylation is required for lipid homeostasis, peroxisome biogenesis, and ER protein quality control.
journal_name
Sci Repjournal_title
Scientific reportsauthors
William James A,Ravi C,Srinivasan M,Nachiappan Vdoi
10.1038/s41598-019-51054-7subject
Has Abstractpub_date
2019-10-09 00:00:00pages
14485issue
1issn
2045-2322pii
10.1038/s41598-019-51054-7journal_volume
9pub_type
杂志文章abstract::We aimed to determine the clinical and microbiological characteristics of Candida bloodstream infections in children and the impact of therapeutic strategies on outcomes. All pediatric patients with candidemia from a medical center in Taiwan over a 13-year period (2003-2015) were included and a total of 262 patients w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01123-6
更新日期:2017-04-24 00:00:00
abstract::We report evidence of the transition from n- to p-type conduction of InN with increasing Mg dopant concentration by using photoconductivity (PC) measurement at room temperature. This transition is depicted as a conversion from negative to positive PC under above-bandgap optical excitation. The n- to p-type transition ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04371
更新日期:2014-03-13 00:00:00
abstract::Objectives were to evaluate the role of canonical WNT signaling in development of the preimplantation embryo. Signaling was activated with 2-Amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidine (AMBMP) and inhibited with Dickkopf-related protein 1 (DKK1). Treatment of bovine embryos with AMBMP at da...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01266
更新日期:2013-01-01 00:00:00
abstract::Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07244-2
更新日期:2017-07-31 00:00:00
abstract::Most glioblastoma patients have a dismal prognosis, although some survive several years. However, only few biomarkers are available to predict the disease course. EGR1 and EGR3 have been linked to glioblastoma stemness and tumour progression, and this study aimed to investigate their spatial expression and prognostic ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66236-x
更新日期:2020-06-09 00:00:00
abstract::Osthole, an active coumarin isolated from Cnidium monnieri (L.) Cusson, has long been used in China as an antipruritic herbal medicine; however, the antipruitic mechanism of osthole is unknown. We studied the molecular mechanism of osthole in histamine-dependent itch by behavioral test, Ca(2+) imaging, and electrophys...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25657
更新日期:2016-05-10 00:00:00
abstract::Inhomogeneity in the ground state is an intriguing, emergent phenomenon in magnetism. Recently, it has been observed in the magnetostructural channel of the geometrically frustrated α-NaMnO2, for the first time in the absence of active charge degrees of freedom. Here we report an in-depth numerical and local-probe exp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09272
更新日期:2015-03-19 00:00:00
abstract::Targeted protein degradation (TPD) through a proteasome-dependent pathway induced by heterofunctional small molecules is initiated by the formation of a ternary complex with recruited E3 ligases. This complex formation affects the degradation ability of TPD molecules, and thus we tested for visualization of the intrac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59966-5
更新日期:2020-02-20 00:00:00
abstract::Apolipoprotein E4 (E4) and type 2 diabetes are major risk factors for cognitive decline and late onset Alzheimer's disease (AD). E4-associated phenotypes and insulin resistance (IR) share several features and appear to interact in driving cognitive dysfunction. However, shared mechanisms that could explain their overl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43701
更新日期:2017-03-08 00:00:00
abstract::Excessive or misdirected artificial light at night (ALAN) produces light pollution that influences several aspects of the biology and ecology of birds, including disruption of circadian rhythms and disorientation during flight. Many migrating birds traverse large expanses of land twice every year at night when ALAN il...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21577-6
更新日期:2018-02-19 00:00:00
abstract::The renal sodium chloride cotransporter, NCC, in the distal convoluted tubule is important for maintaining body Na+ and K+ homeostasis. Endogenous NCC is highly ubiquitylated, but the role of individual ubiquitylation sites is not established. Here, we assessed the role of 10 ubiquitylation sites for NCC function. Tra...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12819-0
更新日期:2017-10-11 00:00:00
abstract::Inorganic semiconductors such as III-V materials are very important in our everyday life as they are used for manufacturing optoelectronic and microelectronic components with important applications span from energy harvesting to telecommunications. In some applications, these components are required to operate in hars...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21864-2
更新日期:2018-02-23 00:00:00
abstract::This is the first study to demonstrate that colour transformations in the volume of a photochromic material (PM) are induced at the intersections of two control light channels, one controlling PM colouration and the other controlling decolouration. Thus, PM colouration is induced by position selectivity, and therefore...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31543
更新日期:2016-08-16 00:00:00
abstract::Nickel-titanium-oxide (Ni-Ti-O) nanotube arrays (NTAs) prepared on nearly equiatomic NiTi alloy shall have broad application potential such as for energy storage and biomedicine, but their precise structure control is a great challenge because of the high content of alloying element of Ni, a non-valve metal that canno...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07547
更新日期:2014-12-18 00:00:00
abstract::The core of the Vibrio Harveyi clade contains V. harveyi, V. campbellii, V. owensii, V. jasicida, and V. rotiferianus. They are well recognized aquatic animal pathogens, but misclassification has been common due to similarities in their rDNA sequences and phenotypes. To better understand their evolutionary relationshi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41394
更新日期:2017-02-01 00:00:00
abstract::Oxidation of squalene (SQ) causes a decline in the nutritional value of SQ in foods, as well as an accumulation of SQ oxidation products in skin lipids which lead to adverse skin conditions. However, mechanistic insights as to how SQ is oxidized by different oxidation mechanisms have been limited, and thus effective m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27455-5
更新日期:2018-06-14 00:00:00
abstract::Sarcopenia is defined as skeletal muscle attenuation and has an association with metabolic syndrome. Metabolic syndrome, which includes obesity, is one of known predictive factors for gastroesophageal reflux disease (GERD). This study aimed to elucidate the association between sarcopenia and GERD. We retrospectively r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56702-6
更新日期:2019-12-27 00:00:00
abstract::TBC1D10C is a protein previously demonstrated to bind and inhibit Ras and Calcineurin. In cardiomyocytes, also CaMKII is inhibited and all three targeted enzymes are known to promote maladaptive cardiomyocyte hypertrophy. Here, in accordance with lack of Calcineurin inhibition in vivo, we did not observe a relevant an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33853
更新日期:2016-09-26 00:00:00
abstract::Fusarium Head Blight (FHB) caused by Fusarium graminearum pathogens constitutes a major threat to agricultural production because it frequently reduces the yield and quality of the crop. The disease severity is predicted to increase in various regions owing to climate change. Integrated management where biocontrol pla...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37718-w
更新日期:2019-01-24 00:00:00
abstract::Stroke incidence is a multifactorial disease and especially hyperhomocysteinemia is associated with a higher risk of stroke. Previous studies have reported a folate metabolism disorder associated with the MTHFR gene. We investigated four single nucleotide polymorphisms in the MTHFR 3'-UTR [2572 C > A (rs4846049), 4869...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12668-x
更新日期:2017-09-29 00:00:00
abstract::Large-scale molecular epidemiologic studies of Plasmodium falciparum parasites have provided insights into parasite biology and transmission, can identify the spread of drug resistance, and are useful in assessing vaccine targets. The polyclonal nature infections in high transmission settings is problematic for tradit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41108
更新日期:2017-01-24 00:00:00
abstract::Ordered/disordered phase separation occurring in bio-membranes has piqued researchers' interest because these ordered domains, called lipid rafts, regulate important biological functions. The structure of the ordered domain has been examined with artificial membranes, which undergo macroscopic ordered/disordered phase...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79083-7
更新日期:2020-12-21 00:00:00
abstract::Malaria has steadily increased in the Peruvian Amazon over the last five years. This study aimed to determine the parasite prevalence and micro-geographical heterogeneity of Plasmodium vivax parasitaemia in communities of the Peruvian Amazon. Four cross-sectional active case detection surveys were conducted between Ma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07818-0
更新日期:2017-08-14 00:00:00
abstract::Birds have a highly specialized and efficient digestive system, but when this system originated remains uncertain. Here we report six gastric pellets attributable to the recently discovered 160-million-year-old troodontid dinosaur Anchiornis, which is among the key taxa for understanding the transition to birds. The g...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32202-x
更新日期:2018-09-21 00:00:00
abstract::Neuroimaging studies of the Psychomotor Vigilance Task (PVT) have revealed brain regions involved in attention lapses in sleep-deprived and well-rested adults. Those studies have focused on individual brain regions, rather than integrated brain networks, and have overlooked adolescence, a period of ongoing brain devel...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50180-6
更新日期:2019-09-26 00:00:00
abstract::The band-edge structure and band gap are key parameters for a functional chalcogenide semiconductor to its applications in optoelectronics, nanoelectronics, and photonics devices. Here, we firstly demonstrate the complete study of experimental band-edge structure and excitonic transitions of monoclinic digallium trisu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06143
更新日期:2014-08-21 00:00:00
abstract::At 50 kg in estimated weight, the extinct Siamogale melilutra is larger than all living otters, and ranks among the largest fossil otters. The biomechanical capability of S. melilutra jaws as related to their large size is unknown but crucial to reconstructing the species' potentially unique ecological niche. Here we ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15391-9
更新日期:2017-11-09 00:00:00
abstract::Most of the human genome is non-coding. However, some of the non-coding part is transcriptionally active. In humans, the tandemly repeated (TR) pericentromeric non-coding DNA-human satellites 2 and 3 (HS2, HS3)-are transcribed in somatic cells. These transcripts are also found in pre- and post-implantation embryos. Th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76628-8
更新日期:2020-11-12 00:00:00
abstract::Methylation of histone H3-lysine 9 (H3K9) and H3K27 by the methyltransferase G9a and polycomb repressive complex 2 (PRC2) inhibits transcription of target genes. A crosstalk between G9a and PRC2 via direct physical interaction has been shown recently. Here, we demonstrate an alternative mechanism by which G9a promotes...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18709
更新日期:2015-12-21 00:00:00
abstract::The nuclear pore complex (NPC) is one of the largest supramolecular structures in eukaryotic cells. Its octagonal ring-scaffold perforates the nuclear envelope and features a unique molecular machinery that regulates nucleocytoplasmic transport. NPCs are composed of ~30 different nucleoporins (Nups), averaged at 8, 16...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09372
更新日期:2015-03-23 00:00:00