Abstract：
:Aconitine (ACO), the main active component in Aconitum carmichaelii Debeaux (family: Ranunculaceae), has high cardiotoxicity, however the mechanisms of this effect remain unclear. Paeoniflorin (PF), the main chemical ingredient in herbaceous peony, can protect the heart from damage through antioxidant, vasodilatory and other effects. In this study, we focused on the mechanism by which PF reduces ACO cardiotoxicity. We selected H9c2 cells as the experimental model. MTT assay, Western blot analysis and real-time PCR were used to measure cell proliferation, apoptosis, ion channels and oxidative stress. Cell proliferation was significantly increased, the Bcl-2/Bax ratio and p53 level were upregulated, and Caspase-3 was slightly reduced in the ACO+PF group compared with the ACO group. SCN5A mRNA expression was significantly increased in the ACO+PF group compared with the ACO group, while RyR2 and Cx43 mRNA expression was decreased. Compared with the ACO group, the ACO+PF group showed marked decreases in extracellular lactate dehydrogenase (LDH) and intracellular malondialdehyde (MDA), while there was no difference in intracellular superoxide dismutase (SOD). The above data demonstrate that the cardiotoxicity of ACO in H9c2 cells was significantly decreased by PF.
journal_name
J Biol Regul Homeost Agentsjournal_title
Journal of biological regulators and homeostatic agentsauthors
Li J,Zhang SH,He D,Wang JF,Li JQdoi
10.23812/19-257Asubject
Has Abstractpub_date
2019-09-01 00:00:00pages
1425-1436issue
5eissn
0393-974Xissn
1724-6083pii
42journal_volume
33pub_type
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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更新日期：2017-10-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
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pub_type： 杂志文章,随机对照试验
doi：
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journal_title：Journal of biological regulators and homeostatic agents
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doi：
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journal_title：Journal of biological regulators and homeostatic agents
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更新日期：2018-01-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
pub_type： 社论,评审
doi：
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journal_title：Journal of biological regulators and homeostatic agents
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更新日期：1993-01-01 00:00:00
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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journal_title：Journal of biological regulators and homeostatic agents
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