Microcomputed Tomography Is a Precise Method That Allows for Topographical Characterization of Lymph Nodes and Lymphatic Vessels.

Abstract:

: Background: Surgical excision and/or radiation targeting of regional lymph nodes are an essential component in the clinical management of cancer. Importantly, a more accurate understanding of lymphatic anatomy could enable refinement of present treatment strategies. Given the spatial resolution limitations of contemporary imaging methods, our group sought to utilize noncontrast-enhanced microcomputed tomography (μCT) imaging to clarify regional lymphatic anatomy. Methods and Results: This study was conducted with embalmed en bloc lymphatic tissue packets from six donors (three females and three males: medianage of death = 78 years). All specimens were investigated with noncontrast-enhanced μCT imaging using a conebeam-CT imaging system. Adipose and lymphatic tissues were segmented by radiodensity based on sampling regions of interest. To confirm the observations from μCT, lymph nodes from each packet were exposed to hematoxylin and eosin staining and anti-D240 immunostaining. Following μCT imaging, mean peak radiodensities of -203.14 ± 19.35 Hounsfield units (HU) and 37.25 ± 31.95 HU were revealed for adipose and lymphatic tissues, respectively (p < 0.01). By analyzing histograms of the radiodensity distributions, we determined a threshold of -82.42 HU to differentiate adipose and lymphatic tissue, to generate three-dimensional renderings, and to calculate quantitative metrics. On average, adipose tissue comprised 9.62 ± 3.60 cm3 (73.6%) of the total packet volume, whereas lymphatic tissue comprised 3.47 ± 2.71 cm3 (26.4%). Moreover, each en bloc packet contained four small lymph nodes (1-5 mm) and three to four large lymph nodes (>5 mm). Histology corroborated the observations from μCT. Conclusions: Altogether, a precise understanding of regional lymphatic anatomy elucidated by the present imaging modality may help refine clinical cancer treatment strategies.

journal_name

Lymphat Res Biol

authors

Fournier DE,Groh AMR,Nair SM,Norley CJD,Pollmann SI,Holdsworth DW,Power NE,Beveridge TS

doi

10.1089/lrb.2019.0007

subject

Has Abstract

pub_date

2020-04-01 00:00:00

pages

166-173

issue

2

eissn

1539-6851

issn

1557-8585

journal_volume

18

pub_type

杂志文章
  • Outcomes in Vascularized Lymph Node Transplantation in Rabbits: A Reliable Model for Improving the Surgical Approach to Lymphedema.

    abstract:: Background: Conservative nonsurgical treatment has traditionally been applied to lymphedema. Early surgical procedures were invasive and disfiguring and their long-term success was often limited. In contrast, contemporary surgical techniques are much less invasive and have...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2018.0038

    authors: Fernández Peñuela R,Casaní Arazo L,Masiá Ayala J

    更新日期:2019-08-01 00:00:00

  • Yellow nail syndrome: treatment of lymphedema using low pressure compression.

    abstract::The present report describes a case with the triad of yellow nail syndrome (YNS) and the use of low-pressure compression pump as treatment of lymphedema in YNS. A 71-year-old woman presented with bilateral lower extremity lymphedema, yellow nails, and recurrent bilateral pleural effusion. In this case, we specifically...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2011.0012

    authors: Polat AK,Dang HT,Soran A

    更新日期:2012-03-01 00:00:00

  • SF-36 Shows Increased Quality of Life Following Complete Reduction of Postmastectomy Lymphedema with Liposuction.

    abstract::Abstracts Background: Arm lymphedema after breast cancer surgery affects women both from physical and psychological points of view. Lymphedema leads to adipose tissue deposition. Liposuction and controlled compression therapy (CCT) reduces the lymphedema completely. METHODS AND RESULTS:Sixty female patients with arm ...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2016.0035

    authors: Hoffner M,Bagheri S,Hansson E,Manjer J,Troëng T,Brorson H

    更新日期:2017-03-01 00:00:00

  • Abdominal lymphatic pump treatment increases leukocyte count and flux in thoracic duct lymph.

    abstract:BACKGROUND:Previous studies suggest that rhythmic compression of the abdomen (abdominal lymphatic pump techniques, LPT) enhances immunity and resistance to infectious disease, but direct evidence of this has not been documented. In this study, the thoracic duct of eight anesthetized mongrel dogs was catheterized, so th...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2007.1001

    authors: Hodge LM,King HH,Williams AG Jr,Reder SJ,Belavadi T,Simecka JW,Stoll ST,Downey HF

    更新日期:2007-01-01 00:00:00

  • Blood Flow Velocity in Brachial and Subclavian Vessels Immediately After Compressive Procedures for Treatment of Postcancer Therapy Lymphedema in Breast Cancer: A Randomized Blind Clinical Trial.

    abstract:OBJECTIVE:This study sought to evaluate the effect of elastic compression, functional compressive bandaging, and kinesiotherapy on blood flow of the upper limb with lymphedema secondary to the treatment of breast cancer. METHODS:This was a randomized blind crossover clinical trial with a washout period of 7 days betwe...

    journal_title:Lymphatic research and biology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1089/lrb.2016.0022

    authors: Rezende MS,Marsengo AL,de Jesus Guirro RR,de Oliveira Guirro EC

    更新日期:2017-03-01 00:00:00

  • Automated measurement of diameter and contraction waves of cannulated lymphatic microvessels.

    abstract:BACKGROUND:Studies of lymphatic function often employ collecting lymphatic vessels that exhibit large-amplitude, spontaneous contractions. Data from such preparations have been analyzed using cardiac pump analogies that require accurate determination of vascular dimensions, including external (OD) and internal (ID) dia...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2006.4.3

    authors: Davis MJ,Zawieja DC,Gashev AA

    更新日期:2006-04-01 00:00:00

  • Lymphatic endothelial cells, lymphangiogenesis, and extracellular matrix.

    abstract::Exciting studies involving the molecular regulation of lymphangiogenesis in lymphatic-associated disorders (e.g., wound healing, lymphedema and tumor metastasis) have focused renewed attention on the intrinsic relationship between lymphatic endothelial cells (LECs) and extracellular matrix (ECM) microenvironment. ECM ...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章,评审

    doi:10.1089/lrb.2006.4.83

    authors: Ji RC

    更新日期:2006-01-01 00:00:00

  • Does Lymphedema Severity Affect Quality of Life? Simple Question. Challenging Answers.

    abstract:PURPOSE:Lymphedema can have a negative impact on the function and quality of life (QOL) of patients, but most studies have examined lymphedema as a binary variable, rather than a multidimensional disease that ranges in severity. This study explored the potential impact of lymphedema severity on function and overall QOL...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2016.0049

    authors: Lee TS,Morris CM,Czerniec SA,Mangion AJ

    更新日期:2018-02-01 00:00:00

  • Macromolecular leak from extrasplenic lymphatics during endotoxemia.

    abstract:BACKGROUND:The spleen has an important physiological role in maintaining blood volume; this study aimed to determine whether during pathophysiological circumstances, namely endotoxemia, the extrasplenic pathway is dysfunctional. We hypothesize that increased 'leakiness' of lymphatics in response to lipopolysaccharide (...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2008.1019

    authors: Brookes ZL,Mansart A,McGown CC,Ross JJ,Reilly CS,Brown NJ

    更新日期:2009-01-01 00:00:00

  • The International LAM Registry: a component of an innovative web-based clinician, researcher, and patient-driven rare disease research platform.

    abstract:BACKGROUND:A relative inability to capture a sufficiently large patient population in any one geographic location has traditionally limited research into rare diseases. METHODS AND RESULTS:Clinicians interested in the rare disease lymphangioleiomyomatosis (LAM) have worked with the LAM Treatment Alliance, the MIT Medi...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2009.0028

    authors: Nurok M,Eslick I,Carvalho CR,Costabel U,D'Armiento J,Glanville AR,Harari S,Henske EP,Inoue Y,Johnson SR,Lacronique J,Lazor R,Moss J,Ruoss SJ,Ryu JH,Seyama K,Watz H,Xu KF,Hohmann EL,Moss F

    更新日期:2010-03-01 00:00:00

  • Test of Responsiveness and Sensitivity of the Questionnaire "Lymphedema Quality of Life Inventory".

    abstract:BACKGROUND:Lymphedema may affect patients' health-related quality of life (HRQoL) and thus a disease-specific questionnaire is needed. The Lymphedema Quality of life Inventory (LyQLI) has been tested for reliability and validity. The purpose of this study was to test the LyQLI for responsiveness and sensitivity. METHO...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章,多中心研究

    doi:10.1089/lrb.2017.0048

    authors: Klernäs P,Johnsson A,Boyages J,Brorson H,Munnoch A,Johansson K

    更新日期:2018-06-01 00:00:00

  • Effects of Aqua-Lymphatic Therapy on Lower Extremity Lymphedema: A Randomized Controlled Study.

    abstract:BACKGROUND:The purpose of this study is to investigate the effects of the aqua-lymphatic therapy (ALT) on unilateral lower extremity lymphedema in the maintenance phase. MATERIALS AND METHODS:This is a randomized controlled trial with a blinded assessor. The study was completed with 30 ALT and 27 control group partici...

    journal_title:Lymphatic research and biology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1089/lrb.2017.0017

    authors: Ergin G,Karadibak D,Sener HO,Gurpinar B

    更新日期:2017-09-01 00:00:00

  • Lymphangiosarcoma complicating extensive congenital mixed vascular malformations.

    abstract::Pediatric hepatic angiosarcoma is a very rare malignant vascular tumor. A few cases have shown pediatric hepatic angiosarcoma occurring on a background of preexisting vascular lesions. We report the case of a newborn girl who presented extensive limbs and upper trunk cutaneous mixed vascular malformations at birth. Th...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2009.0034

    authors: Al Dhaybi R,Agoumi M,Powell J,Dubois J,Kokta V

    更新日期:2010-09-01 00:00:00

  • Clinical Benefits of Supervised Home Care of the Morbidity Management and Disability Prevention Under National Filariasis Elimination Programme.

    abstract:: Introduction: Mass drug administration and morbidity management and disability prevention (MMDP) though considered as two pillars of global program for elimination of filariasis, implementation of MMDP was disregarded in several endemic countries. Limb hygiene is the main ...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2019.0058

    authors: De Britto L,Kamaraj P,Vijayalakshmi G,Das LK,Krishnakumari A,Anbusivam S,Supriya VK,Yuvaraj J

    更新日期:2020-12-01 00:00:00

  • Determining the precision of dual energy x-ray absorptiometry and bioelectric impedance spectroscopy in the assessment of breast cancer-related lymphedema.

    abstract:BACKGROUND:The composition of breast cancer-related lymphedema (BCRL) has been shown to evolve from the initial accumulation of fluid to the development of fibrotic lesions and abnormal fat deposition. Therefore, precise and reliable assessments of BCRL are required to develop accurate staging and management. Although ...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2012.0020

    authors: Newman AL,Rosenthall L,Towers A,Hodgson P,Shay CA,Tidhar D,Vigano A,Kilgour RD

    更新日期:2013-06-01 00:00:00

  • Lymphedema Leads to Fat Deposition in Muscle and Decreased Muscle/Water Volume After Liposuction: A Magnetic Resonance Imaging Study.

    abstract:BACKGROUND:Lymphedema leads to adipose tissue deposition. Water-fat magnetic resonance imaging (MRI) can quantify and localize fat and water. The presence of excess fat and excess water/muscle in the subfascial compartment of the lymphedematous limb has not been investigated before. The aim of this study was to investi...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2017.0042

    authors: Hoffner M,Peterson P,Månsson S,Brorson H

    更新日期:2018-04-01 00:00:00

  • Lymphedema Impact and Prevalence International Study: The Canadian Data.

    abstract:: Background: Chronic edema/lymphedema is defined as edema present for more than 3 months. It is underrecognized and undertreated. The International Lymphedema Framework developed an international study, Lymphedema Impact and Prevalence International (LIMPRINT), to estimate ...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2019.0014

    authors: Keast DH,Moffatt C,Janmohammad A

    更新日期:2019-04-01 00:00:00

  • Serum Immune Proteins in Limb Lymphedema Reflecting Tissue Processes Caused by Lymph Stasis and Chronic Dermato-lymphangio-adenitis (Cellulitis).

    abstract:BACKGROUND:Lymphedema of limbs affects a large mass of tissues. Pathological changes develop in skin and subcutaneous tissue. Bacterial retention in edema fluid is followed by chronic inflammatory reaction. The question arises whether the chronic processes affecting a large mass of limb tissues are reflected in the ser...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2017.0003

    authors: Zaleska MT,Olszewski WL

    更新日期:2017-09-01 00:00:00

  • Comparison of intermittent pneumatic compression with manual lymphatic drainage for treatment of breast cancer-related lymphedema.

    abstract:BACKGROUND:The aim of this prospective controlled study was to assess the efficacy of two different combination treatment modalities of lymphedema (LE). Manual lymphatic drainage (MLD) and compression bandage combination (complex decongestive therapy) have been compared with intermittent pneumatic compression (IPC) plu...

    journal_title:Lymphatic research and biology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1089/lrb.2012.0002

    authors: Gurdal SO,Kostanoglu A,Cavdar I,Ozbas A,Cabioglu N,Ozcinar B,Igci A,Muslumanoglu M,Ozmen V

    更新日期:2012-09-01 00:00:00

  • Assessment of segmental arm soft tissue composition in breast cancer-related lymphedema: a pilot study using dual energy X-ray absorptiometry and bioimpedance spectroscopy.

    abstract:BACKGROUND:Changes in arm soft tissue composition, especially increased adipose tissue, has been found in advanced, non-pitting breast cancer-related lymphedema (BCRL). The aim of this study was to examine whether these changes were localized to any particular region of the arm and whether they occurred in lymphedema w...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2014.0033

    authors: Czerniec SA,Ward LC,Meerkin JD,Kilbreath SL

    更新日期:2015-03-01 00:00:00

  • Diagnosing Male Unilateral Upper Limb Lymphedema: Determination of Normatively Determined Thresholds Using Bioimpedance Spectroscopy.

    abstract:: Background: The use of bioimpedance spectroscopy (BIS) for the detection of unilateral upper limb lymphedema is growing. Currently, normatively determined diagnostic thresholds using bioimpedance are available for females only. It is unclear if they are suitable for males ...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2018.0051

    authors: Xie AZT,Lee MJ,Dylke E

    更新日期:2020-02-01 00:00:00

  • Risk Factors Related to Lower Limb Edema, Compression, and Physical Activity During Pregnancy: A Retrospective Study.

    abstract:OBJECTIVE:The aim of the article was to assess risk factors and to analyze methods applied in the prevention and treatment of lower limb edema in pregnant women with a particular focus on compression therapy and exercise. MATERIALS AND METHODS:Fifty-four women during the early 24-hour period following delivery were as...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2016.0038

    authors: Ochalek K,Pacyga K,Curyło M,Frydrych-Szymonik A,Szygula Z

    更新日期:2017-06-01 00:00:00

  • Spontaneous electrical activity in sheep mesenteric lymphatics.

    abstract:BACKGROUND:It has recently become apparent that the lymph pump is an electrical entity that rivals the heart in complexity. Many interesting currents have been demonstrated by voltage clamping isolated lymphatic smooth muscle cells, but until now the role of these currents in the intact syncitium has not been studied. ...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2007.5104

    authors: Beckett EA,Hollywood MA,Thornbury KD,McHale NG

    更新日期:2007-01-01 00:00:00

  • Postoperative evolution of thickness and echogenicity of cutis and subcutis of patients with and without breast cancer-related lymphedema.

    abstract:BACKGROUND:Compared to volume/circumference measurements to diagnose lymphedema, thickening and disturbance of echogenicity of cutis and subcutis associated with the development of lymphedema is measured directly by ultrasonography. The aim of the present study was to investigate the evolution of thickness and echogeni...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2013.0028

    authors: Devoogdt N,Pans S,De Groef A,Geraerts I,Christiaens MR,Neven P,Vergote I,Van Kampen M

    更新日期:2014-03-01 00:00:00

  • Extracellular RNA Profile in Mesenteric Lymph from Exemplar Rat Models of Acute and Critical Illness.

    abstract:: Background: Mesenteric lymph (ML) has been implicated in the development of multiple organ dysfunction syndrome in critical illness. Extracellular RNAs play a role in cell-to-cell communication during physiological and disease processes but they are rarely studied in ML. W...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2018.0044

    authors: Hong J,Blenkiron C,Tsai P,Premkumar R,Nachkebia S,Tun SM,Petzer A,Windsor JA,Hickey AJ,Phillips AR

    更新日期:2019-10-01 00:00:00

  • Acute Cardiovascular Responses to the Application of Manual Lymphatic Drainage in Different Body Regions.

    abstract:: Background: The purpose of this study was to investigate acute cardiovascular responses to manual lymphatic drainage (MLD) on different parts of the body. Materials and Methods: Thirty healthy individuals (10 women and 20 men) ...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2018.0043

    authors: Esmer M,Keser I,Erer D,Kupeli B

    更新日期:2019-06-01 00:00:00

  • Electrode Equivalence for Use in Bioimpedance Spectroscopy Assessment of Lymphedema.

    abstract:BACKGROUND:Bioimpedance spectroscopy (BIS) is commonly used in the assessment and monitoring of lymphedema. This study investigated electrodes as a source of variability that could impact the accuracy of BIS in the clinic and determined if Ag/AgCl electrocardiograph (ECG) electrodes could be used as an alternative to i...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2018.0002

    authors: Svensson BJ,Dylke ES,Ward LC,Kilbreath SL

    更新日期:2019-02-01 00:00:00

  • Primary Lymphedema of the Upper Extremities: Clinical and Lymphoscintigraphic Features in 23 Patients.

    abstract:BACKGROUND:Primary idiopathic lymphedema is an uncommon condition that typically affects the lower extremities. Patients have a malformed lymphatic system that causes subcutaneous fluid and adipose deposition. Rarely, the disease also has been described in the upper extremities. The purpose of this study was to investi...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2017.0085

    authors: Goss JA,Maclellan RA,Greene AK

    更新日期:2019-02-01 00:00:00

  • A novel approach to slow down putrefaction of unembalmed cadaveric tissue during lymphatic dissection: a preliminary study.

    abstract:BACKGROUND:The putrefaction factor is one of the biggest problems when undertaking unembalmed cadaveric tissue dissections for lymphatic vessel mapping. METHODS:One female bilateral breast and anterior upper torso from an unembalmed human cadaver was studied over an 8-week period. Multiple prefrozen domestic ice brick...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2008.1020

    authors: Pan WR

    更新日期:2009-01-01 00:00:00

  • Vascular Alterations in Axillary and Brachial Vessels in Patients with Axillary Web Syndrome After Breast Cancer Surgery.

    abstract:BACKGROUND:Surgical manipulations of the axilla may cause a condition known as Axillary Web Syndrome (AWS). The systems compromised and the sequence of events leading to this syndrome remains unknown. This study evaluated clinical, surgical, and vascular factors associated with onset and duration of AWS after breast ca...

    journal_title:Lymphatic research and biology

    pub_type: 杂志文章

    doi:10.1089/lrb.2017.0037

    authors: Furlan C,Matheus CN,Jales RM,Derchain S,Sarian LO

    更新日期:2018-06-01 00:00:00