Abstract:
:Introduction: Currently, genetic testing of BRCA1/2 genes includes screening for single-nucleotide variants, small insertions/deletions, and copy number variations (CNVs). In fact, many studies document the involvement of BRCA1/2 gene rearrangements in genetic predisposition to breast and ovarian cancer. Large genomic rearrangements (LGRs) of BRCA1 may account for up to one-third of all disease-causing alterations in various populations, while LGRs in BRCA2 are less frequently observed. Areas covered: We aimed to present an overview of current technologies employed in molecular diagnosis of BRCA1/2 LGRs. The most relevant literature papers, showing the application of new strategies, were considered. Expert opinion: Currently, the progress of next-generation sequencing (NGS) technologies allows for the validation of new pipelines able to provide rapid and effective results, ensuring the sensitivity and specificity requested for the detection of BRCA1/2 LGRs. Multiplex ligation-dependent probe amplification remains the gold standard to confirm NGS CNVs results and to perform fast screening in families where a pathogenic rearrangement has been detected in a proband.
journal_name
Expert Rev Mol Diagnjournal_title
Expert review of molecular diagnosticsauthors
Concolino P,Capoluongo Edoi
10.1080/14737159.2019.1657011subject
Has Abstractpub_date
2019-09-01 00:00:00pages
795-802issue
9eissn
1473-7159issn
1744-8352journal_volume
19pub_type
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章,评审
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journal_title:Expert review of molecular diagnostics
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journal_title:Expert review of molecular diagnostics
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journal_title:Expert review of molecular diagnostics
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journal_title:Expert review of molecular diagnostics
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pub_type: 社论
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章,评审
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章,评审
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更新日期:2016-08-01 00:00:00
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章,评审
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更新日期:2017-02-01 00:00:00
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journal_title:Expert review of molecular diagnostics
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更新日期:2018-03-01 00:00:00
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章,评审
doi:10.1586/14737159.2014.868307
更新日期:2014-03-01 00:00:00
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章,评审
doi:10.1586/14737159.7.6.805
更新日期:2007-11-01 00:00:00
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章,评审
doi:10.1586/14737159.5.5.781
更新日期:2005-09-01 00:00:00
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章,评审
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更新日期:2017-04-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Expert review of molecular diagnostics
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章,评审
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更新日期:2009-10-01 00:00:00
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journal_title:Expert review of molecular diagnostics
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章
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journal_title:Expert review of molecular diagnostics
pub_type: 杂志文章,评审
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journal_title:Expert review of molecular diagnostics
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journal_title:Expert review of molecular diagnostics
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