Abstract：
:Steroidal C19-hydroxylation is pivotal to the synthesis of naturally occurring bioactive C19-OH steroids and 19-norsteroidal pharmaceuticals. However, realizing this transformation is proved to be challenging through either chemical or biological synthesis. Herein, we report a highly efficient method to synthesize 19-OH-cortexolone in 80% efficiency at the multi-gram scale. The obtained C19-OH-cortexolone can be readily transformed to various synthetically useful intermediates including the industrially valuable 19-OH-androstenedione, which can serve as a basis for synthesis of C19-functionalized steroids as well as 19-nor steroidal drugs. Using this biocatalytic C19-hydroxylation method, the unified synthesis of six C19-hydroxylated pregnanes is achieved in just 4 to 9 steps. In addition, the structure of sclerosteroid B is revised on the basis of our synthesis.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Wang J,Zhang Y,Liu H,Shang Y,Zhou L,Wei P,Yin WB,Deng Z,Qu X,Zhou Qdoi
10.1038/s41467-019-11344-0subject
Has Abstractpub_date
2019-07-29 00:00:00pages
3378issue
1issn
2041-1723pii
10.1038/s41467-019-11344-0journal_volume
10pub_type
杂志文章abstract：:Human Bestrophin1 (hBest1) is a Ca2+-activated Cl- channel in retinal pigment epithelium (RPE) essential for retina physiology, and its mutation results in retinal degenerative diseases that have no available treatments. Here, we discover that hBest1's channel activity in human RPE is significantly enhanced by adenosi...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-05616-4
更新日期：2018-08-07 00:00:00
abstract：:GFI1 is a transcriptional regulator expressed in lymphoid cells, and an "oncorequisite" factor required for development and maintenance of T-lymphoid leukemia. GFI1 deletion causes hypersensitivity to ionizing radiation, for which the molecular mechanism remains unknown. Here, we demonstrate that GFI1 is required in T...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-03817-5
更新日期：2018-04-12 00:00:00
abstract：:Ubiquitous satellite communications are in a leading position for bridging the digital divide. Fulfilling such a mission will require satellite services on par with fibre services, both in bandwidth and cost. Achieving such a performance requires a new generation of communications payloads powered by large-scale proce...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-10077-4
更新日期：2019-04-30 00:00:00
abstract：:Malaria is a major public health problem that is actively being addressed in a global eradication campaign. Increased population mobility through international air travel has elevated the risk of re-introducing parasites to elimination areas and dispersing drug-resistant parasites to new regions. A simple genetic mark...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms5052
更新日期：2014-06-13 00:00:00
abstract：:Sepsis is a life-threatening condition triggered by a dysregulated host response to microbial infection resulting in vascular dysfunction, organ failure and death. Here we provide a semi-quantitative atlas of the murine vascular cell-surface proteome at the organ level, and how it changes during sepsis. Using in vivo ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-12672-x
更新日期：2019-10-11 00:00:00
abstract：:To fully exploit the microbial genome resources, a high-throughput experimental platform is needed to associate genes with phenotypes at the genome level. We present here a novel method that enables investigation of the cellular consequences of repressing individual transcripts based on the CRISPR interference (CRISPR...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-04899-x
更新日期：2018-06-26 00:00:00
abstract：:Circuit reactivations involve a stereotyped sequence of neuronal firing and have been behaviourally linked to memory consolidation. Here we use multiphoton imaging and patch-clamp recording, and observe sparse and stereotyped circuit reactivations that correspond to UP states within active neurons. To evaluate the eff...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms3574
更新日期：2013-01-01 00:00:00
abstract：:Somatic mutations in the calreticulin (CALR) gene are associated with approximately 30% of essential thrombocythemia (ET) and primary myelofibrosis (PMF). CALR mutations, including the two most frequent 52 bp deletion (del52) and 5 bp insertion (ins5), induce a frameshift to the same alternative reading frame generati...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-18691-3
更新日期：2020-09-28 00:00:00
abstract：:Neuroimaging modalities such as MRI and EEG are able to record from the whole brain, but this comes at the price of either limited spatiotemporal resolution or limited sensitivity. Here, we show that functional ultrasound imaging (fUS) of the brain is able to assess local changes in cerebral blood volume during cognit...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-09349-w
更新日期：2019-03-28 00:00:00
abstract：:Oxygenic photosynthetic organisms perform solar energy conversion of water and CO2 to O2 and sugar at a broad range of wavelengths and light intensities. These cells also metabolize sugars using a respiratory system that functionally overlaps the photosynthetic apparatus. In this study, we describe the harvesting of p...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-04613-x
更新日期：2018-06-04 00:00:00
abstract：:Cell fate determination requires the cooperation between extrinsic signals and intrinsic molecules including transcription factors as well as epigenetic regulators. Nevertheless, how neural fate commitment is regulated by epigenetic modifications remains largely unclear. Here we show that transient histone deacetylati...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms7830
更新日期：2015-04-23 00:00:00
abstract：:Merons are nontrivial topological spin textures highly relevant for many phenomena in solid state physics. Despite their importance, direct observation of such vortex quasiparticles is scarce and has been limited to a few complex materials. Here, we show the emergence of merons and antimerons in recently discovered tw...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-20497-2
更新日期：2021-01-08 00:00:00
abstract：:Scaffolded DNA origami has proven to be a powerful and efficient technique to fabricate functional nanomachines by programming the folding of a single-stranded DNA template strand into three-dimensional (3D) nanostructures, designed to be precisely motion-controlled. Although two-dimensional (2D) imaging of DNA nanoma...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-03018-0
更新日期：2018-02-09 00:00:00
abstract：:Autophagy can degrade cargos with the help of selective autophagy receptors such as p62/SQSTM1, which facilitates the degradation of ubiquitinated cargo. While the process of autophagy has been linked to aging, the impact of selective autophagy in lifespan regulation remains unclear. We have recently shown in Caenorha...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-13540-4
更新日期：2019-12-11 00:00:00
abstract：:The Cdc45-MCM-GINS (CMG) helicase unwinds DNA during the elongation step of eukaryotic genome duplication and this process depends on the MCM ATPase function. Whether CMG translocation occurs on single- or double-stranded DNA and how ATP hydrolysis drives DNA unwinding remain open questions. Here we use cryo-electron ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms10708
更新日期：2016-02-18 00:00:00
abstract：:Controlling the transmission of electrical current using a quantum point contact constriction paved a way to a large variety of experiments in mesoscopic physics. The increasing interest in heat transfer in such systems fosters questions about possible manipulations of quantum heat modes that do not carry net charge (...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-02433-z
更新日期：2017-12-21 00:00:00
abstract：:Orexins are associated with drug relapse in rodents. Here, we show that acute restraint stress in mice activates lateral hypothalamic (LH) orexin neurons, increases levels of orexin A and 2-arachidonoylglycerol (2-AG) in the ventral tegmental area (VTA), and reinstates extinguished cocaine-conditioned place preference...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms12199
更新日期：2016-07-22 00:00:00
abstract：:Among the perovskite oxide family, KTaO3 (KTO) has recently attracted considerable interest as a possible system for the realization of the Rashba effect. In this work, we report a novel conducting interface by placing KTO with another insulator, LaVO3 (LVO) and report planar Hall effect (PHE) and anisotropic magnetor...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-14689-z
更新日期：2020-02-13 00:00:00
abstract：:The role of microglia in spinal cord injury (SCI) remains poorly understood and is often confused with the response of macrophages. Here, we use specific transgenic mouse lines and depleting agents to understand the response of microglia after SCI. We find that microglia are highly dynamic and proliferate extensively ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-08446-0
更新日期：2019-01-31 00:00:00
abstract：:Bulk photovoltaic effect (BPVE), featuring polarization-dependent uniform photoresponse at zero external bias, holds potential for exceeding the Shockley-Queisser limit in the efficiency of existing opto-electronic devices. However, the implementation of BPVE has been limited to the naturally existing materials with b...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-20115-1
更新日期：2020-12-17 00:00:00
abstract：:Nitric oxide (NO) has important functions in biology and atmospheric chemistry as a toxin, signaling molecule, ozone depleting agent and the precursor of the greenhouse gas nitrous oxide (N2O). Although NO is a potent oxidant, and was available on Earth earlier than oxygen, it is unclear whether NO can be used by micr...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-09268-w
更新日期：2019-03-20 00:00:00
abstract：:Genetic noise together with genome duplication and volume changes during cell cycle are significant contributors to cell-to-cell heterogeneity. How can cells buffer the effects of these unavoidable epigenetic and genetic variations on phenotypes that are sensitive to such variations? Here we show that a simple network...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms12959
更新日期：2016-10-03 00:00:00
abstract：:Models of visual attention postulate the existence of a saliency map whose function is to guide attention and gaze to the most conspicuous regions in a visual scene. Although cortical representations of saliency have been reported, there is mounting evidence for a subcortical saliency mechanism, which pre-dates the ev...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms14263
更新日期：2017-01-24 00:00:00
abstract：:Underlying cellular responses is a transcriptional regulatory network (TRN) that modulates gene expression. A useful description of the TRN would decompose the transcriptome into targeted effects of individual transcriptional regulators. Here, we apply unsupervised machine learning to a diverse compendium of over 250 ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-13483-w
更新日期：2019-12-04 00:00:00
abstract：:The preparation of α-functionalized organic acids can be greatly simplified by adopting a protocol involving the catalytic assembly of achiral building blocks. However, the enzymatic assembly of small amino acids and aldehydes to form numerous α-functionalized organic acids is highly desired and remains a significant ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-06241-x
更新日期：2018-09-19 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature communications
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41467-020-15800-0
更新日期：2020-04-20 00:00:00
abstract：:Engineering lattice thermal conductivity requires to control the heat carried by atomic vibration waves, the phonons. The key parameter for quantifying it is the phonon lifetime, limiting the travelling distance, whose determination is however at the limits of instrumental capabilities. Here, we show the achievement o...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-00584-7
更新日期：2017-09-08 00:00:00
abstract：:Accurately predicting chromatin loops from genome-wide interaction matrices such as Hi-C data is critical to deepening our understanding of proper gene regulation. Current approaches are mainly focused on searching for statistically enriched dots on a genome-wide map. However, given the availability of orthogonal data...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-17239-9
更新日期：2020-07-09 00:00:00
abstract：:The social intelligence hypothesis (SIH) posits that within-group interactions drive cognitive evolution, but it has received equivocal support. We argue the SIH overlooks a major component of social life: interactions with conspecific outsiders. Competition for vital resources means conspecific outsiders present myri...
journal_title：Nature communications
pub_type： 杂志文章,评审
doi：10.1038/s41467-020-18780-3
更新日期：2020-10-06 00:00:00
abstract：:Here, to overcome many limitations accompanying current available methods to detect protein-protein interactions (PPIs), we develop a live cell method called Split Intein-Mediated Protein Ligation (SIMPL). In this approach, bait and prey proteins are respectively fused to an intein N-terminal fragment (IN) and C-termi...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-16299-1
更新日期：2020-05-15 00:00:00
