Abstract:
:At the post-synaptic density (PSD), large protein complexes dynamically form and dissociate in response to synaptic activity, comprising the biophysical basis for learning and memory. The use of detergents to isolate the PSD and release its membrane-associated proteins complicates studies of these activity-dependent protein interaction networks, because detergents can simultaneously disrupt the very interactions under study. Despite widespread recognition that different detergents yield different experimental results, the effect of detergent on activity-dependent synaptic protein complexes has not been rigorously examined. Here, we characterize the effect of three detergents commonly used to study synaptic proteins on activity-dependent protein interactions. We first demonstrate that SynGAP-containing interactions are more abundant in 1% Deoxycholate (DOC), while Shank-, Homer- and mGluR5-containing interactions are more abundant in 1% NP-40 or Triton. All interactions were detected preferentially in high molecular weight complexes generated by size exclusion chromatography, although the detergent-specific abundance of proteins in high molecular weight fractions did not correlate with the abundance of detected interactions. Activity-dependent changes in protein complexes were consistent across detergent types, suggesting that detergents do not isolate distinct protein pools with unique behaviors. However, detection of activity-dependent changes is more or less feasible in different detergents due to baseline solubility. Collectively, our results demonstrate that detergents affect the solubility of individual proteins, but activity-dependent changes in protein interactions, when detectable, are consistent across detergent types.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Lautz JD,Gniffke EP,Brown EA,Immendorf KB,Mendel RD,Smith SEPdoi
10.1038/s41598-019-46690-ysubject
Has Abstractpub_date
2019-07-26 00:00:00pages
10890issue
1issn
2045-2322pii
10.1038/s41598-019-46690-yjournal_volume
9pub_type
杂志文章abstract::DNA double-strand breaks pose a direct threat to genomic stability. Studies of DNA damage and chromatin dynamics have yielded opposing results that support either increased or decreased chromatin motion after damage. In this study, we independently measure the dynamics of transcriptionally active or repressed chromati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36343-x
更新日期:2018-12-27 00:00:00
abstract::Transgenic plants producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are useful for pest control, but their efficacy is reduced when pests evolve resistance. Here we examined the mechanism of resistance to Bt toxin Cry1Ac in the laboratory-selected LF5 strain of the cotton bollworm, Helicov...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07219
更新日期:2014-11-27 00:00:00
abstract::Stem and progenitor cells of the submandibular salivary gland (SMG) give rise to, maintain, and regenerate the multiple lineages of mature epithelial cells including those belonging to the ductal, acinar, basal and myoepithelial subtypes. Here we have exploited single cell RNA-sequencing and in vivo genetic lineage tr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32343-z
更新日期:2018-09-19 00:00:00
abstract::DNA condensation is a ubiquitous phenomenon in biology, yet the physical basis for it has remained elusive. Here, we have explored the mechanism of DNA condensation through the protamine-DNA interaction, and by examining on it the influence of DNA binding drugs. We observed that the DNA condensation is accompanied by ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41975-8
更新日期:2019-04-10 00:00:00
abstract::Psychological and behavioural interventions may be effective in reducing menopause-related symptoms. This randomized controlled trial aimed to evaluate the effectiveness of Mindfulness-based Stress Reduction (MBSR) in reducing menopause-related symptoms by comparing with an active control group, the menopause educatio...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1038/s41598-018-24945-4
更新日期:2018-04-26 00:00:00
abstract::Skin allergy is a chronic condition that affects about 20% of the population of the western world. This disease is caused by small reactive compounds, haptens, able to penetrate into the epidermis and modify endogenous proteins, thereby triggering an immunogenic reaction. Phenyl isothiocyanate (PITC) and ethyl isothio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21203
更新日期:2016-02-17 00:00:00
abstract::The order Chlamydiales are biphasic intracellular bacterial pathogens infecting humans and domesticated animals. Wildlife infections have also been reported, with the most studied example being Chlamydia pecorum infections in the koala, an iconic Australian marsupial. In koalas, molecular evidence suggests that spill-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13164-y
更新日期:2017-10-09 00:00:00
abstract::Although intestinal trefoil factor (ITF) can alleviate the burn-induced intestinal mucosa injury, the underlying mechanisms remains elusive. In this study, we investigated if ITF alters glutamine transport on the brush border membrane vesicles (BBMVs) of the intestines in Sprague-Dawley rats inflicted with 30% TBSA an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21282-4
更新日期:2018-02-16 00:00:00
abstract::Nitrate treatment has been widely used in various seawater injection projects to treat biologic sulfate reduction or reservoir souring. To design a promising nitrate treatment plan, it is essential to have a comprehensive understanding of reactions that represent the microbial communities of the reservoir and mechanis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44033-5
更新日期:2019-05-17 00:00:00
abstract::Recent data suggest that tumor laterality and mucinous histology may be clinically relevant. We investigated how both variables impact on the prognosis and the response to therapies in a large population-based cohort of cancer patients. Incidence data, clinical and pathological features, and outcome were systematicall...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40096-6
更新日期:2019-03-06 00:00:00
abstract::The oral environment affects not only oral health, but also general health, and the importance of oral self-care has recently been recognised. Although toothbrushes are the most important self-care product, there are few toothbrushes that have an inhibitory effect on oral bacteria. In the present study, monofilaments ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80646-x
更新日期:2021-01-08 00:00:00
abstract::Studies of personality have suggested that dissimilarities in ability to cope with stressful situations results in differing tendency to develop addictive behaviors. The present study used selectively bred stress-resilient, socially-dominant (Dom) and stress-vulnerable, socially-submissive (Sub) mice to investigate th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19816-x
更新日期:2018-01-29 00:00:00
abstract::Coronin7 (CRN7) stabilizes F-actin and is a regulator of processes associated with the actin cytoskeleton. Its loss leads to defects in phagocytosis, motility and development. It harbors a CRIB (Cdc42- and Rac-interactive binding) domain in each of its WD repeat domains which bind to Rac GTPases preferably in their GD...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14437
更新日期:2015-09-28 00:00:00
abstract::High-performance locally resonant metamaterials represent the next frontier in materials technology due to their extraordinary properties obtained through materials design, enabling a variety of potential applications. The most exceptional feature of locally resonant metamaterials is the subwavelength size of their un...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68894-3
更新日期:2020-07-21 00:00:00
abstract::17α-ethynilestradiol (EE2) and tamoxifen (Tmx) are pollutants world-wide distributed in aquatic environments. Gilthead seabream, Sparus aurata L., is highlighted as a species model of intensively culture in anthropogenic disturbed environments. The effects of these pollutants on gilthead seabream reproduction and some...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64522-2
更新日期:2020-05-14 00:00:00
abstract::The state of preservation of wood in two samples from the Hanson Logboat, currently on display in Derby Museum and Art Gallery, was analysed using elemental analysis (EA), pyrolysis-gas chromatography/flame ionisation detection (Py-GC/FID), pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) and scanning electro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14057-w
更新日期:2017-10-20 00:00:00
abstract::Stem cell-derived cardiomyocytes provide a promising tool for human developmental biology, regenerative therapies, disease modeling, and drug discovery. As human pluripotent stem cell-derived cardiomyocytes remain functionally fetal-type, close monitoring of electrophysiological maturation is critical for their furthe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43210
更新日期:2017-03-07 00:00:00
abstract::Distillers grains are co-products of the corn ethanol industry widely used in animal feed. We examined the effects of erythromycin, penicillin, and virginiamycin at low concentrations reflective of those detected in distillers grains on bacterial resistance selection. At 0.1 µg/ml erythromycin, macrolide-resistant mut...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09593-4
更新日期:2017-09-08 00:00:00
abstract::Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a kind of primary cardiomyopathy characterized by the fibro-fatty replacement of right ventricular myocardium. Currently, myocardial microRNAs have been reported to play critical role in the pathophysiology of cardiovascular pathophysiology. So far, the profili...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28101
更新日期:2016-06-16 00:00:00
abstract::Recently, minocycline, a tetracycline antibiotic, has been reported to improve symptoms of psychiatric disorders and to facilitate sober decision-making in healthy human subjects. Here we show that minocycline also reduces the risk of the 'honey trap' during an economic exchange. Males tend to cooperate with physicall...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01685
更新日期:2013-01-01 00:00:00
abstract::Tudor domain containing protein 3 (TDRD3) is a modular protein identified based on its ability to recognize methylated arginine motifs through its Tudor domain. We have previously shown that TDRD3 localizes to cytoplasmic stress granules, a structure shown to promote survival upon treatment with chemotherapeutic drugs...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04955-4
更新日期:2017-07-11 00:00:00
abstract::In 2017-2019 a surge of Shispare Glacier, a former tributary of the once larger Hasanabad Glacier (Hunza region), dammed the proglacial river of Muchuhar Glacier, which formed an ice-dammed lake and generated a small Glacial Lake Outburst Flood (GLOF). Surge movement produced the highest recorded Karakoram glacier sur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61277-8
更新日期:2020-03-13 00:00:00
abstract::The growth of epitaxial Si nanowires by a metal-catalyst-free process has been investigated as an alternative to the more common metal-catalyzed vapor-liquid-solid process. The well-aligned Si nanowires are successfully grown on a (111)-oriented Si substrate without any metal catalysts by a thermal treatment using sil...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30608
更新日期:2016-07-28 00:00:00
abstract::The control of structural and chemical transitions in bimetallic nanoalloys at finite temperatures is one of the challenges for their use in advanced applications. Comparing Nested Sampling and Molecular Dynamics simulations, we investigate the phase changes of CuPt nanoalloys with the aim to elucidate the role of kin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27308-1
更新日期:2018-06-14 00:00:00
abstract::Despite dedicated research has been carried out to adequately map the distribution of the sperm whale in the Mediterranean Sea, unlike other regions of the world, the species population status is still presently uncertain. The analysis of two years of continuous acoustic data provided by the ANTARES neutrino telescope...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45517
更新日期:2017-04-12 00:00:00
abstract::Albacore tuna (Thunnus alalunga) is an important target of tuna fisheries in the Atlantic and Indian Oceans. The commercial catch of albacore is the highest globally among all temperate tuna species, contributing around 6% in weight to global tuna catches over the last decade. The accurate assessment and management of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72369-w
更新日期:2020-09-24 00:00:00
abstract::Ti-24Nb-4Zr-8Sn (Ti2448), a new β-type Ti alloy, consists of nontoxic elements and exhibits a low uniaxial tensile elastic modulus of approximately 45 GPa for biomedical implant applications. Nevertheless, the bio-corrosion resistance and biocompatibility of Ti2448 alloys must be improved for long-term clinical use. I...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24590-x
更新日期:2018-04-26 00:00:00
abstract::Quantum information science addresses how the processing and transmission of information are affected by uniquely quantum mechanical phenomena. Combination of two-qubit gates has been used to realize quantum circuits, however, scalability is becoming a critical problem. The use of three-qubit gates may simplify the st...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45353
更新日期:2017-03-31 00:00:00
abstract::Recent studies demonstrate the functions of long non-coding RNAs (lncRNAs) in mediating gene expression at the transcriptional or translational level. Our previous study identified a Sirt1 antisense (AS) lncRNA transcribed from the Sirt1 AS strand. However, its role and regulatory mechanism is still unknown in myogene...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21865
更新日期:2016-02-23 00:00:00
abstract::The ZIC proteins are a family of transcription regulators with a well-defined zinc finger DNA-binding domain and there is evidence that they elicit functional DNA binding at a ZIC DNA binding site. Little is known, however, regarding domains within ZIC proteins that confer trans-activation or -repression. To address t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69917-9
更新日期:2020-08-04 00:00:00