Abstract：
:Chronic suppurative otitis media (CSOM) is one of the most common infectious diseases of the middle ear especially affecting children, leading to delay in language development and communication. Although Staphylococcus aureus is the most common pathogen associated with CSOM, its interaction with middle ear epithelial cells is not well known. In the present study, we observed that otopathogenic S. aureus has the ability to invade human middle ear epithelial cells (HMEECs) in a dose and time dependent manner. Scanning electron microscopy demonstrated time dependent increase in the number of S. aureus on the surface of HMEECs. We observed that otopathogenic S. aureus primarily employs a cholesterol dependent pathway to colonize HMEECs. In agreement with these findings, confocal microscopy showed that S. aureus colocalized with lipid rafts in HMEECs. The results of the present study provide new insights into the pathogenesis of S. aureus induced CSOM. The availability of in vitro cell culture model will pave the way to develop novel effective treatment modalities for CSOM beyond antibiotic therapy.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Mittal R,Debs LH,Patel AP,Nguyen D,Blackwelder P,Yan D,Weckwerth PH,Liu XZdoi
10.1038/s41598-019-47079-7subject
Has Abstractpub_date
2019-07-25 00:00:00pages
10777issue
1issn
2045-2322pii
10.1038/s41598-019-47079-7journal_volume
9pub_type
杂志文章abstract：:Converging evidence has proved the attentional bias of Internet addicts (IAs) on network information. However, previous studies have neither explained how characteristics of network information are detected by IAs with priority nor proved whether this advantage is in line with the unconscious and automatic process. To...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25442-4
更新日期：2018-06-12 00:00:00
abstract：:Increased risk of comorbidities has been reported in Rheumatic and Musculoskeletal Diseases (RMD). We aimed to evaluate the prevalence and pattern of comorbidities in RMD patients nationwide, to identify multimorbidity clusters and to evaluate the gap between recommendations and real screening. Cross-sectional, multic...
journal_title：Scientific reports
pub_type： 杂志文章,多中心研究
doi：10.1038/s41598-020-64732-8
更新日期：2020-05-06 00:00:00
abstract：:Electrochemical nanosensors based on nanoporous gold leaf (NPGL) and molecularly imprinted polymer (MIP) are developed for pharmaceutical analysis by using metronidazole (MNZ) as a model analyte. NPGL, serving as the loading platform for MIP immobilization, possesses large accessible surface area with superb electric ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07699
更新日期：2015-01-09 00:00:00
abstract：:Carbon nanotubes (CNTs) are among the most versatile nanomaterials, but their exploitation is hindered by limited dispersibility, especially in aqueous solvents. Here, we show that AP-LYS, a highly cationic soluble derivative of denatured hen egg lysozyme, is a very effective tool for the unbundling and solubilisation...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52701-9
更新日期：2019-11-12 00:00:00
abstract：:The Southern Ocean has taken up more than 40% of the total anthropogenic carbon (Cant) stored in the oceans since the preindustrial era, mainly in subantarctic mode and intermediate waters (SAMW-AAIW). However, the physical mechanisms responsible for the transfer of Cant into the ocean interior remain poorly understoo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17292-3
更新日期：2017-12-06 00:00:00
abstract：:Denitrification in coastal sediments can provide resilience to eutrophication in estuarine ecosystems, but this key ecosystem function is impacted directly and indirectly by increasing stressors. The erosion and loading of fine sediments from land, resulting in sedimentation and elevated sediment muddiness, presents a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54192-0
更新日期：2019-11-26 00:00:00
abstract：:Historical records indicate that extensive cultural, commercial and technological interaction occurred between European and Asian populations. What have been the biological consequences of these contacts in terms of gene flow? We systematically estimated gene flow between Eurasian groups using genome-wide polymorphism...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09500
更新日期：2015-04-02 00:00:00
abstract：:Gastrointestinal (g.i.) large cell lymphoma is currently regarded as diffuse large B-cell lymphoma (DLBCL) despite a more favorable clinical outcome compared to other DLBCL. Cluster analyses on a transcriptome signature of NF-κB target genes of 30 g.i. marginal zone B-cell lymphomas (MZBL; 8 g.i. MZBL, 22 large cell M...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05116-3
更新日期：2017-07-20 00:00:00
abstract：:Plant seed germination is a crucial developmental event that has significant effects on seedling establishment and yield production. This process is controlled by multiple intrinsic signals, particularly phytohormones. The gaseous hormone ethylene stimulates seed germination; however, the genetic basis of ethylene pro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58607-1
更新日期：2020-02-03 00:00:00
abstract：:Centrioles are essential for forming cilia, flagella and centrosomes. Successful centriole assembly requires proteins of the SAS-6 family, which can form oligomeric ring structures with ninefold symmetry in vitro. While important progress has been made in understanding SAS-6 protein biophysics, the mechanisms enabling...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27075
更新日期：2016-06-08 00:00:00
abstract：:Pharmacotherapy provision based on Nicotine Metabolite Ratio (NMR) status (slow/normal metabolism) may improve smoking cessation rates. However, it is unclear whether NMR status is consistent across patient characteristics and current treatment choice. Data come from 1,826 participants attending Stop Smoking Services ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17994-8
更新日期：2017-12-14 00:00:00
abstract：:The annotation of protein function is a vital step to elucidate the essence of life at a molecular level, and it is also meritorious in biomedical and pharmaceutical industry. Developments of sequencing technology result in constant expansion of the gap between the number of the known sequences and their functions. Th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37179
更新日期：2016-11-16 00:00:00
abstract：:The establishment of clinically relevant models for tumor metastasis and drug testing is a major challenge in cancer research. Here we report a physiologically relevant assay enabling quantitative analysis of metastatic capacity of tumor cells following implantation into the chorioallantoic membrane (CAM). Engraftment...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73632-w
更新日期：2020-10-09 00:00:00
abstract：:Topological quantum computation has been extensively studied in the past decades due to its robustness against decoherence. One way to realize the topological quantum computation is by adiabatic evolutions-it requires relatively long time to complete a gate, so the speed of quantum computation slows down. In this work...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22077
更新日期：2016-02-25 00:00:00
abstract：:We assessed the association between depression status and prevalent and incident type 2 diabetes (T2D) as well as the interaction between depression and a genetic risk score (GS) based on 20 T2D single-nucleotide polymorphisms (SNPs) in a multi-ethnic longitudinal study. We studied 17,375 participants at risk for dysg...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01406-y
更新日期：2017-05-09 00:00:00
abstract：:Non-classical crystallisation (NCC) pathways are widely accepted, however there is conflicting evidence regarding the intermediate stages of crystallisation, how they manifest and further develop into crystals. Evidence from direct observations is especially lacking for small organic molecules, as distinguishing these...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75937-2
更新日期：2020-11-05 00:00:00
abstract：:Improvement of the critical current density (Jc) of superconducting wires/tapes is one of the key issues in the field of superconductivity applications. Here we report the fabrication of a silver-sheathed Ba1-xNaxFe2As2 (BaNa-122) superconducting tape by using a powder-in-tube technique and its superconducting propert...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49363-y
更新日期：2019-09-10 00:00:00
abstract：:The Irish Travellers are a population with a history of nomadism; consanguineous unions are common and they are socially isolated from the surrounding, 'settled' Irish people. Low-resolution genetic analysis suggests a common Irish origin between the settled and the Traveller populations. What is not known, however, i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42187
更新日期：2017-02-09 00:00:00
abstract：:CRISPR-Cas9 is a powerful new tool for genome editing, but this technique creates mosaic mutations that affect the efficiency and precision of its ability to edit the genome. Reducing mosaic mutations is particularly important for gene therapy and precision genome editing. Although the mechanisms underlying the CRSIPR...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42081
更新日期：2017-02-03 00:00:00
abstract：:Although the effects of osteoporosis on the skeleton are well studied, site-specific and long-term studies on the mandible are still lacking. This study investigated the time-course changes of the bone microarchitecture in the mandibular condyle in comparison to the corresponding changes in the alveolar bone, body of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50554-w
更新日期：2019-10-02 00:00:00
abstract：:Inflammation plays central roles in the immune response. Inflammatory response normally requires higher energy and therefore is associated with glucose metabolism. Our recent study demonstrates that lncRNA HOTAIR plays key roles in NF-kB activation, cytokine expression, and inflammation. Here, we investigated if HOTAI...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80291-4
更新日期：2021-01-08 00:00:00
abstract：:A new simple method is proposed to extract the ambipolar diffusion length for two-dimensional (2D) electronic transport in thin film structures using a scanning photoluminescence microscopy (SPLM) setup. No spatially-resolved photoluminescence detection methods are required. By measuring the excitation-position-depend...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62093-w
更新日期：2020-03-23 00:00:00
abstract：:Vaginal microbiota is an important early source of bacterial colonization for newborns. However, only a few small studies have investigated the composition of vaginal microbiota during labor. In this work, we analyzed vaginal swabs collected at 36 weeks gestation and at the onset of labor from 256 women participating ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17972-0
更新日期：2017-12-14 00:00:00
abstract：:Breast cancer survivors display altered inflammatory responses to immune challenges relative to cancer-naive controls likely due to previous cancer treatments, stress associated with cancer, and/or tumor physiology. Proper inflammatory responses are necessary for adaptive sickness behaviors (e.g., fatigue, anorexia, a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37334-8
更新日期：2019-01-24 00:00:00
abstract：:We have employed a model system, inspired by SNARE proteins, to facilitate membrane fusion between Giant Unilamellar Vesicles (GUVs) and Large Unilamellar Vesicles (LUVs) under physiological conditions. In this system, two synthetic lipopeptide constructs comprising the coiled-coil heterodimer-forming peptides K4, (KI...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59926-z
更新日期：2020-02-20 00:00:00
abstract：:Soil zinc (Zn) plays a crucial role in plant growth, but excessive accumulation in the environment may lead to air, water and soil pollution. It is affected by various chemical, environmental and spatial factors. Therefore, it is important to identify the factors influencing Zn content in the landscape. The main motiv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33745-9
更新日期：2018-10-22 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-71840-y
更新日期：2020-09-01 00:00:00
abstract：:In many real-world networks, the ability to withstand targeted or global attacks; extinctions; or shocks is vital to the survival of the network itself, and of dependent structures such as economies (for financial networks) or even the planet (for ecosystems). Previous attempts to characterise robustness include neste...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59959-4
更新日期：2020-02-25 00:00:00
abstract：:Sex-based variations in the immune response to the influenza vaccines was reported, however, the genetic basis responsible for the sex variations in the immune response toward the influenza vaccines remains unclear. Here, the genes responsible for sex-specific responses after vaccination with trivalent inactivated inf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31999-x
更新日期：2018-09-10 00:00:00
abstract：:Pulmonary Arterial Hypertension (PAH) is a rare and progressive disease with low incidence and prevalence, and elevated mortality. PAH is characterized by increased mean pulmonary artery pressure. The aim of this study was to analyse patients with combined mutations in BMPR2, ACVRL1, ENG and KCNA5 genes and to establi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33570
更新日期：2016-09-15 00:00:00
