Abstract:
:Monoclonal antibodies to factor VIII antigen (VIII:Ag) and von Willebrand factor (vWf:Ag) were immobilised on Sephacryl S-1000 and tested for their ability to deplete normal human citrated plasma of factor VIII. A combination of two antibodies to VIII:Ag and one antibody to vWf:Ag was required to produce plasma containing less than 0.01 IU/ml. Its performance in the one stage coagulation assay of VIII:C was equivalent to that of congenital VIII deficient plasma for the assay of normal and haemophilic plasma and factor VIII concentrates. Storage of freeze dried aliquots of this product at -20 degrees C, +4 degrees C, and 37 degrees C showed that it could be used as a substrate for at least six months when stored at temperatures +4 degrees C and below.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Hornsey VS,Waterston YG,Prowse CVdoi
10.1136/jcp.41.5.562subject
Has Abstractpub_date
1988-05-01 00:00:00pages
562-7issue
5eissn
0021-9746issn
1472-4146journal_volume
41pub_type
杂志文章abstract::Experience after 18 months' work with the SMA-4 system of multiple analysis for routine haematology is described, as well as the modifications to the machine which have been made for smoother operation. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.22.1.19
更新日期:1969-01-01 00:00:00
abstract:AIMS:Meticillin-resistant Staphylococcus aureus (MRSA) strains isolated in Tawam Hospital, a tertiary care hospital in the United Arab Emirates, were examined in order to understand the reasons for a doubling of its incidence between 2003 and 2008 while maintaining the same infection control measures. METHODS:All cons...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200436
更新日期:2012-02-01 00:00:00
abstract::Three hundred and sixty samples of blood from 230 hospital patients were examined and compared with the results of simultaneous blood culture to determine the value of buffy-coat microscopy in detecting bacteraemia. One observer found 86 positive smears, 12 of which were from patients with positive blood cultures and ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.12.1375
更新日期:1981-12-01 00:00:00
abstract:AIMS:To develop a specific radioimmunoassay for the routine determination of serum vitamin B12. METHODS:Antisera were raised in rabbits by immunisation with the monocarboxylic acid derivative of cyanocobalamin coupled to human serum albumin. Antibody titres and affinities were determined and the antiserum giving the h...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.4.328
更新日期:1992-04-01 00:00:00
abstract::The cytological features of testicular germ cell tumours were established in smears from 15 freshly resected tumours. These features were applied to the fine needle aspiration cytology diagnosis of metastases in 27 patients referred for chemotherapy. There were 16 positive reports in 32 aspirates of which 13 were take...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.11.1324
更新日期:1987-11-01 00:00:00
abstract::A new sensitive nitrite test, the SRN test, was evaluated for its suitability as a reliable screening technique for the detection of bacteriuria. The SRN test was compared to a currently available nitrite test, the Microstix nitrite test, and the results obtained with both nitrite tests were assessed in comparison wit...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.7.723
更新日期:1981-07-01 00:00:00
abstract::Zygomycosis is not often diagnosed in the United Kingdom, and so the possible importance of the findings in a patient with disseminated zygomycosis who had been treated with chemotherapy for erythroleukaemia was not appreciated until histological examination of specimens obtained at necropsy provided a presumptive dia...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.9.1039
更新日期:1985-09-01 00:00:00
abstract:BACKGROUND:Clinical outcome in patients with diffuse large B cell lymphomas (DLBCL) is highly variable and poorly predictable. Microarray studies showed that patients with DLBCL with a germinal centre B cell-like (GCB) phenotype have a better prognosis than those with an activated B cell-like (ABC) phenotype. The BMI1 ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.038752
更新日期:2007-02-01 00:00:00
abstract:AIM:To assess the topographical relation between gastric glands, using the minimum spanning tree (MST), to derive both a model of neighbourhood and quantitative representation of the tissue's architecture, to assess the characteristic features of gastric atrophy, and to assess the grades of gastric atrophy. METHODS:Ha...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.12.895
更新日期:1998-12-01 00:00:00
abstract::An automated technique is described which is capable of detecting sickle-cell haemoglobin and differentiating the sickle-cell trait from sickle-cell anaemia. The method is based upon the Itano solubility test and utilizes Technicon equipment. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.4.330
更新日期:1972-04-01 00:00:00
abstract:AIMS:Positive direct antiglobulin tests (DATs) are valuable in identifying the aetiology of autoimmune haemolysis and in guiding therapeutic intervention. However, in HIV-positive individuals with background polyclonal gammopathy, a positive DAT in the absence of haemolysis is common. In this setting, IgG quantificatio...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-207113
更新日期:2020-12-16 00:00:00
abstract:AIMS:To examine the correlation between bromodeoxyuridine (BrdU) labelling indices (LI) and tubular damage in renal biopsy specimens; to evaluate the diagnostic and prognostic potential of measuring cell proliferation in a variety of renal lesions. METHODS:In vitro BrdU labelling of renal biopsy specimens was undertak...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.12.1085
更新日期:1994-12-01 00:00:00
abstract::A rare case of adult onset Langerhans' cell histiocytosis associated with dysgerminoma in a 35 year old Chinese woman is reported. The patient had a history of dysgerminoma of left ovary 15 years previously and had undergone surgery followed by radiotherapy and an uneventful recovery. She presented again in March 1994...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.10.963
更新日期:1995-10-01 00:00:00
abstract::A chemical test for the detection of bacteriuria-Uriglox-has been compared with the results obtained on culturing urine obtained by suprapubic aspiration in 351 pregnant or puerperal women. The results of this comparison show a correlation of 96.9%, both false positive and false negative results occurring. ...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1136/jcp.26.5.375
更新日期:1973-05-01 00:00:00
abstract:AIMS:Endotoxin detection assays are not validated for use in end-stage kidney disease (ESKD). We investigated the accuracy and precision of the kinetic turbidimetric Limulus amoebocyte lysate (LAL) assay to detect endotoxin in plasma from patients with ESKD. Optimisation of endotoxin recovery from plasma using the dete...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202622
更新日期:2015-01-01 00:00:00
abstract:AIMS:To establish whether abnormalities in the course of the vertebral artery occur and whether they are relevant to arterial injury associated with head and neck movements. METHODS:Twenty vertebral arteries were carefully dissected at necropsy and abnormalities in course were noted, along with any other bony or carti...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.7.637
更新日期:1995-07-01 00:00:00
abstract::The clinical and pathological features of a case of malakoplakia of the adrenal gland occurring in a woman with Escherichia coli infection are described. This lesion mimicked a neoplasm, the true diagnosis only being revealed by histological examination. The light and electron microscopic features are described and it...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.6.606
更新日期:1981-06-01 00:00:00
abstract:AIMS:To investigate the problems involved in undertaking immunohistochemistry (IHC) and nuclear morphometry using Bouin's fixed prostate biopsies. METHODS:Archival Bouin's fixed and formalin fixed, paraffin wax embedded prostatic biopsies were immunostained for three nuclear biomarkers (minichromosome maintenance prot...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.019299
更新日期:2005-03-01 00:00:00
abstract:AIMS:The miR-9 family microRNAs (miRNAs) are tumour suppressor miRNAs implicated in carcinogenesis. We postulated that miR-9-1, miR-9-2 and miR-9-3 may be inactivated by aberrant promoter methylation in multiple myeloma (MM). METHODS:Methylation of miR-9-1, miR-9-2 and miR-9-3 was studied by methylation-specific PCR (...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202817
更新日期:2015-07-01 00:00:00
abstract:AIMS:Evidence suggests that the presence of tumour necrosis is an adverse prognostic factor in renal cell carcinoma (RCC). However, it has also been shown that tumour regression, a microscopic feature associated with necrosis, may be a favourable short term prognostic factor in RCC. METHODS:Pathology reports of 253 RC...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.018846
更新日期:2005-01-01 00:00:00
abstract:AIMS:To determine whether myelodysplastic syndromes (MDS) are associated with sea blue histiocytosis in the bone marrow. METHODS:A retrospective review of bone marrow aspirates from 35 patients presenting consecutively with MDS and from 20 patients with each of the following: normal marrow appearance (routine staging ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.11.1030
更新日期:1993-11-01 00:00:00
abstract::In an investigation of the in vitro phagocytic and microbicidal function of granulocytes collected by filtration leucapheresis (FL) from 18 donors and by discontinuous flow centrifugation leucapheresis (DFC) from six donors, comparison was made with the function of granulocytes obtained from the same donors by venepun...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.5.586
更新日期:1983-05-01 00:00:00
abstract::Inhibition of streptolysin O activity by cholesterol depends on the latter being in free form. The normal esterified and protein-bound cholesterol fractions in serum do not influence streptolysin O activity. However, high cholesterol levels in rabbits fed cholesterol with cholic acid were associated with an increased ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.10.885
更新日期:1972-10-01 00:00:00
abstract::Twelve methods for the demonstration of bacterial penicillinase production by strains of Haemophilus influenzae and Staphylococcus aureus are compared, and their suitability for routine clinical laboratory use is evaluated. The acidometric agar plate method is recommended. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.10.1061
更新日期:1979-10-01 00:00:00
abstract:BACKGROUND:Cardiac remodelling after acute myocardial infarction (AMI) is characterised by molecular and cellular mechanisms involving both left and right ventricles, and biventricular failure identifies patients with an extremely unfavourable prognosis. AIMS:To assess whether a link exists between increased myocardia...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.56.9.672
更新日期:2003-09-01 00:00:00
abstract::A study has been made of 523 strains of staphylococci on the basis of biological properties, phage typing, and serology. The value of serology in the identification of pathogenic staphylococci has been assessed. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.4.370
更新日期:1970-05-01 00:00:00
abstract::The T(4)-free thyroxine index was used as the thyroid function test of first choice in a district general hospital for one year. Ninety-two patients were assessed both initially and at follow up by a single physician. The index agreed with clinical assessment in 69 out of 74 patients in whom diagnosis was unequivocal....
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.3.259
更新日期:1972-03-01 00:00:00
abstract::Fifteen cases of large-cell lymphoma, diagnosed as centroblastic (5), B-immunoblastic (5) or true histiocytic (5). lymphoma and one case of malignant histiocytosis were studied with monoclonal antibodies. Each diagnosis was based on morphological as well as marker studies. A panel of monoclonal and heterologous antibo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.1.44
更新日期:1983-01-01 00:00:00
abstract::Breast fibroepithelial lesions, including fibroadenomas and phyllodes tumours, are commonly encountered in clinical practice. As histological differences between these two related entities may be subtle, resulting in a challenging differential diagnosis, pathological techniques to assist the differential diagnosis of ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-203231
更新日期:2015-12-01 00:00:00
abstract::The placenta is the fetal organ providing the interchange between mother and fetus. This organ needs to provide its function such as transport and secretion even during its development and thus all developmental changes need to be in accordance with its function. This review describes development of the placenta durin...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2008.055277
更新日期:2008-12-01 00:00:00