Abstract:
BACKGROUND:Endothelial progenitor cells (EPCs) were indicated in vascular repair, angiogenesis of ischemic organs, and inhibition of formation of initial hyperplasia. Differentiation of endothelial cells (ECs) from human induced pluripotent stem cells (hiPSC)-derived endothelial cells (hiPSC-ECs) provides an unlimited supply for clinical application. Furthermore, magnetic cell labelling offers an effective way of targeting and visualization of hiPSC-ECs and is the next step towards in vivo studies. METHODS:ECs were differentiated from hiPSCs and labelled with uncoated superparamagnetic iron-oxide nanoparticles (uSPIONs). uSPION uptake was compared between hiPSC-ECs and mature ECs isolated from patients by software analysis of microscopy pictures after Prussian blue cell staining. The acute and long-term cytotoxic effects of uSPIONs were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay) and Annexin assay. RESULTS:We showed, for the first time, uptake of uncoated SPIONs (uSPIONs) by hiPSC-ECs. In comparison with mature ECs of identical genetic background hiPSC-ECs showed lower uSPION uptake. However, all the studied endothelial cells were effectively labelled and showed magnetic properties even with low labelling concentration of uSPIONs. uSPIONs prepared by microwave plasma synthesis did not show any cytotoxicity nor impair endothelial properties. CONCLUSION:We show that hiPSC-ECs labelling with low concentration of uSPIONs is feasible and does not show any toxic effects in vitro, which is an important step towards animal studies.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Salingova B,Simara P,Matula P,Zajickova L,Synek P,Jasek O,Veverkova L,Sedlackova M,Nichtova Z,Koutna Idoi
10.3390/ijms20143536subject
Has Abstractpub_date
2019-07-19 00:00:00issue
14issn
1422-0067pii
ijms20143536journal_volume
20pub_type
杂志文章abstract::Methylation of several lysine residues of histones is a crucial mechanism for relatively long-term regulation of genomic activity. Recent molecular biological studies have demonstrated that the function of histone methylation is more diverse and complex than previously thought. Moreover, studies using newly available ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18071404
更新日期:2017-06-30 00:00:00
abstract::The combination of an antifungal agent and drug carrier with adjunctive antimicrobial properties represents novel strategy of complex therapy in pharmaceutical technology. The goal of this study was to investigate the unmodified and ion cross-linked chitosan's influence on anti-Candida activity of clotrimazole used as...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151017765
更新日期:2014-09-30 00:00:00
abstract::Protein kinase is a novel therapeutic target for human diseases. The off-target and side effects of ATP-competitive inhibitors preclude them from the clinically relevant drugs. The compounds targeting the druggable allosteric sites outside the highly conversed ATP binding pocket have been identified as promising alter...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19010111
更新日期:2018-01-01 00:00:00
abstract::One of the most important adaptations of seagrasses during sea colonization was the capacity to grow at the low micromolar nitrate concentrations present in the sea. In contrast to terrestrial plants that use H+ symporters for high-affinity NO3- uptake, seagrasses such as Zostera marina L. use a Na+-dependent high-aff...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20153650
更新日期:2019-07-26 00:00:00
abstract::The wide employment of iron nanoparticles in environmental and occupational settings underlines their potential to enter the brain. Human cell-based systems are recommended as relevant models to reduce uncertainty and to improve prediction of human toxicity. This study aimed at demonstrating the in vitro differentiati...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21010271
更新日期:2019-12-31 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is a solid tumor composed by a genotypically and phenotypically heterogeneous population of neoplastic cells types. High recurrence rate and regional metastases lead to major morbidity and mortality. Recently, many studies have focused on cellular and molecular mechanisms ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18091849
更新日期:2017-08-24 00:00:00
abstract::GABARAP (γ-aminobutyric acid type A receptor-associated protein) and its paralogues GABARAPL1 and GABARAPL2 comprise a subfamily of autophagy-related Atg8 proteins. They are studied extensively regarding their roles during autophagy. Originally, however, especially GABARAPL2 was discovered to be involved in intra-Golg...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010085
更新日期:2020-12-23 00:00:00
abstract::Molecular imprinted polymers (MIP) are considered one of the most promising selective and novel separation methods for removal phenolic compound in wastewater treatment. MIP are crosslinked polymeric materials that exhibit high binding capacity and selectivity towards a target molecule (template), purposely present du...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms10010354
更新日期:2009-01-01 00:00:00
abstract::The worldwide increase in grapevine trunk diseases, mainly esca, represents a major threat for vineyard sustainability. Biocontrol of a pioneer fungus of esca, Phaeomoniella chlamydospora, was investigated here by deciphering the tripartite interaction between this trunk-esca pathogen, grapevine and the biocontrol-oom...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21186876
更新日期:2020-09-19 00:00:00
abstract::Cementum is a mineralized layer on the tooth's root surface and facilitates the biomechanical anchoring of fibrous connective tissues as a part of tooth-supportive complexes. Previously, we observed that OCCM30 cementoblasts cultured on fibrin matrices underwent apoptosis due to fibrin degradation through the expressi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18112380
更新日期:2017-11-09 00:00:00
abstract::Glioblastoma (GBM) is an extremely aggressive tumor of the central nervous system, with a prognosis of 12-15 months and just 3-5% of survival over 5 years. This is mainly because most patients suffer recurrence after treatment that currently consists in maximal resection followed by radio- and chemotherapy with temozo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20112746
更新日期:2019-06-04 00:00:00
abstract::Circular dichroism (CD) is a useful technique for monitoring changes in the conformation of antimicrobial peptides or gelatin. In this study, interactions between cationic peptides and gelatin were observed without affecting the triple helical content of the gelatin, which was more strongly affected by anionic surfact...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13033229
更新日期:2012-01-01 00:00:00
abstract::Burn-induced heart dysfunction is a key factor for patient mortality. However, the molecular mechanisms are not yet fully elucidated. This study sought to understand whether burn-induced heart dysfunction is associated with cardiac mitochondrial dysfunction and interruption of the PDE5A-cGMP-PKG pathway. Sixty percent...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21072350
更新日期:2020-03-28 00:00:00
abstract::We evaluated the association between prostate cancer non-coding RNA 1 (PRNCR1) polymorphisms and the risk of developing gastric cancer (GC) and GC subgroups in Korea. A case-control study was conducted with 437 GC patients and 357 healthy controls using a TaqMan genotyping assay. A chi-squared test, binary logistic re...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20133355
更新日期:2019-07-08 00:00:00
abstract::Presently, many studies have focused on exploring in silico approaches in the identification and development of alternative therapy for the treatment and management of cancer. Solute carrier family-2-member-4-gene (Slc2a4) which encodes glucose transporter 4 protein (GLUT4), has been identified as a promising therapeu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19020386
更新日期:2018-01-28 00:00:00
abstract::The genetic diversity and population genetic structure of 252 accessions from 21 Prunus sibirica L. populations were investigated using 10 ISSR, SSR, and SRAP markers. The results suggest that the entire population has a relatively high level of genetic diversity, with populations HR and MY showing very high diversity...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15010377
更新日期:2013-12-31 00:00:00
abstract::To design ARC-111 analogues with improved efficiency, we constructed the QSAR of 22 ARC-111 analogues with RPMI8402 tumor cells. First, the optimized support vector regression (SVR) model based on the literature descriptors and the worst descriptor elimination multi-roundly (WDEM) method had similar generalization as ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13011161
更新日期:2012-01-01 00:00:00
abstract::Recently, we reported an inhibitory effect of guanine substitutions on the conformational switch from antiparallel to parallel quadruplexes (G4) induced by dehydrating agents. As a possible cause, we proposed a difference in the sensitivity of parallel and antiparallel quadruplexes to the guanine substitutions in the ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21176123
更新日期:2020-08-25 00:00:00
abstract::ADAM9 (A disintegrin and a metalloprotease 9) is a membrane-anchored protein that participates in a variety of physiological functions, primarily through the disintegrin domain for adhesion and the metalloprotease domain for ectodomain shedding of a wide variety of cell surface proteins. ADAM9 influences the developme...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21207790
更新日期:2020-10-21 00:00:00
abstract::Insulin resistance (IR) is one of the key contributing factors in the development of type 2 diabetes mellitus (T2DM). However, the molecular mechanisms leading to IR are still unclear. The implication of microRNAs (miRNAs) in the pathophysiology of multiple cardiometabolic pathologies, including obesity, atherosclerot...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21228675
更新日期:2020-11-17 00:00:00
abstract::Alzheimer's disease (AD) is one of the most frequently diagnosed types of dementia in the elderly. An important pathological feature in AD is the aggregation and deposition of the β-amyloid (Aβ) in extracellular plaques. Transthyretin (TTR) can cleave Aβ, resulting in the formation of short peptides with less activity...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21239003
更新日期:2020-11-26 00:00:00
abstract::The bone-derived hormone fibroblast growth factor 23 (FGF23) acts in concert with parathyroid hormone (PTH) and the active vitamin D metabolite calcitriol in the regulation of calcium (Ca) and phosphate (P) homeostasis. More factors are being identified to regulate FGF23 levels and the endocrine loops between the thre...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21228810
更新日期:2020-11-20 00:00:00
abstract::Activation of the cannabinoid CB1 receptor induces different cellular signaling cascades through coupling to different effector proteins (G-proteins and β-arrestins), triggering numerous therapeutic effects. Conformational changes and rearrangements at the intracellular domain of this GPCR receptor that accompany liga...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218111
更新日期:2020-10-30 00:00:00
abstract::Pasting properties are important characteristics of barley starch from a processing standpoint. Many studies reported the close relationship between pasting properties and malting quality, especially malt extract. However, most conclusions were derived from the correlation between pasting properties and malting qualit...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19113559
更新日期:2018-11-12 00:00:00
abstract::The kidney plays a primary role in maintaining homeostasis and detoxification of numerous hydrophilic xenobiotics as well as endogenous compounds. Because the kidney is exposed to a larger proportion and higher concentration of drugs and toxins than other organs through the secretion of ionic drugs by tubular organic ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17111826
更新日期:2016-11-01 00:00:00
abstract::During their lifespan, Red blood cells (RBC), due to their inability to self-replicate, undergo an ageing degradation phenomenon. This pathway, both in vitro and in vivo, consists of a series of chemical and morphological modifications, which include deviation from the biconcave cellular shape, oxidative stress, membr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19092582
更新日期:2018-08-30 00:00:00
abstract::Experiments were carried out to investigate the effects of various factors on the activity and conformation of recombinant leucine aminopeptidase of Bacillus kaustophilus CCRC 11223 (BkLAP) and potential utilization of BkLAP in the hydrolysis of anchovy protein. Optimal temperature and pH of BkLAP were 70 °C and 8.0 i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12117609
更新日期:2011-01-01 00:00:00
abstract::Dysbiosis, an imbalance of intestinal flora, can cause serious conditions such as obesity, cancer, and psychoneurological disorders. One cause of dysbiosis is inflammation. Ulcerative enteritis is a side effect of non-steroidal anti-inflammatory drugs (NSAIDs). To counteract this side effect, we proposed the concurren...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218166
更新日期:2020-10-31 00:00:00
abstract::Maize can form symbiotic relationships with arbuscular mycorrhiza (AM) fungus to increase productivity and resistance, but the miRNAs in maize responsible for this process have not been discovered. In this study, 155 known and 28 novel miRNAs were identified by performing high-throughput sequencing of sRNA in maize ro...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19103201
更新日期:2018-10-16 00:00:00
abstract::Myeloid immune cells, such as dendritic cells, monocytes, and macrophages, play a central role in the generation of immune responses and thus are often either disabled or even hijacked by tumors. These new tolerogenic activities of tumor-associated myeloid cells are controlled by an oncogenic transcription factor, sig...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19061803
更新日期:2018-06-19 00:00:00