Abstract:
:Subendothelial cells (SEC) were obtained from the inner intimal layer of adult human aorta by collagenase treatment. SEC were identified in primary culture either as smooth muscle cells by staining with FITC-labeled antisera against human smooth muscle myosin or as macrophages, foam cells and contaminating endothelial cells by their uptake of malondialdehyde treated low density lipoproteins labeled with fluorescent dye 3,3'-dioctadecylindocarbocyanine. Between 1 and 5 days in culture, along with smooth muscle cells (SMC, 38-82%), endothelial cells (0-9%), macrophages and foam cells (2-32%), one more type of cell was found. This cell type resembled SMC in size and shape, but was not stained by antisera to SMC myosin. By ultrastructural criteria these cells were characterized as modulated SMC for they contained prominent rough endoplastic reticulum and Golgi complex together with basement membrane and a large number of plasmalemmal vesicles. Like SMC they reacted with phalloidin and were stained by anti-vimentin but not by anti-desmin monoclonal antibodies. The proportion of such cells varied from 5 to 33% of total cell number and increased in parallel to macrophages and foam cells in vessels with well developed atherosclerotic lesions. We conclude that the applied technique may be used for identification of cultured vascular cells including modulated SMC.
journal_name
Atherosclerosisjournal_title
Atherosclerosisauthors
Babaev VR,Antonov AS,Zacharova OS,Romanov YA,Krushinsky AV,Tsibulsky VP,Shirinsky VP,Repin VS,Smirnov VNdoi
10.1016/0021-9150(88)90301-2subject
Has Abstractpub_date
1988-05-01 00:00:00pages
45-56issue
1eissn
0021-9150issn
1879-1484pii
0021-9150(88)90301-2journal_volume
71pub_type
杂志文章相关文献
ATHEROSCLEROSIS文献大全abstract::MGL-3196 is an oral, liver-targeted selective agonist for the thyroid hormone receptor-β (THR-β) that is being developed for the treatment of dyslipidemia. The safety profile and tolerability of THR-β agonist MGL-3196 was assessed in first-in humans studies, including a single ascending dose study (NCT01367873) in whi...
journal_title:Atherosclerosis
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.atherosclerosis.2013.07.056
更新日期:2013-10-01 00:00:00
abstract:RATIONALE:Lipoprotein lipase (LPL) X447 homozygotes are characterized by enhanced conversion of TRL apoB100. Here, we set out to investigate whether this LPL variant is also associated with enhanced apoB48 clearance. Therefore, we evaluated apoB48 kinetics in X447 homozygotes in the fed state by infusion of isotope L-[...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2006.08.038
更新日期:2007-10-01 00:00:00
abstract:BACKGROUND:Dyslipidemia has been linked to vascular complications of Type 1 diabetes (T1DM). We investigated the prospective associations of nuclear magnetic resonance-determined lipoprotein subclass profiles (NMR-LSP) and conventional lipid profiles with carotid intima-media thickness (IMT) in T1DM. METHODS:NMR-LSP a...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2015.10.106
更新日期:2016-01-01 00:00:00
abstract::The hydrolysis of lipoprotein triglycerides is catalyzed by lipoprotein lipase (LPL), an enzyme found in many tissues. We have examined tissue LPL activity (LPLA) in rats with experimentally-induced hyperthyroidism. In younger, lighter rats, hyperthyroidism was accompanied by a decrease in LPL in adipose tissue wherea...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(77)90088-0
更新日期:1977-03-01 00:00:00
abstract::Variation in the APOA5 gene has been shown to be associated with triglyceride levels in several independent population studies. It was our objective to determine if a relationship existed between selected genotypes or haplotypes of the APOA5 gene and findings on selective coronary angiography (SCA) in an independent c...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2004.04.024
更新日期:2004-09-01 00:00:00
abstract::While statin treatment may transiently mobilize endothelial progenitor cells (EPCs), the dose-dependent effects of a continuous statin therapy on EPCs in patients with chronic coronary artery disease (CAD) have not been analyzed. In 209 patients with angiographically documented CAD, 144 of which received 10-40 mg/day ...
journal_title:Atherosclerosis
pub_type: 临床试验,杂志文章
doi:10.1016/j.atherosclerosis.2006.05.031
更新日期:2007-06-01 00:00:00
abstract:BACKGROUND AND AIMS:Cardiovascular disease (CVD) and cancer are the two leading causes of death in smokers. Lung cancer screening is recommended in a large proportion of smokers. We examined the implication of coronary artery calcium (CAC) score (quantitative and qualitative) for cardiovascular disease (CVD), coronary ...
journal_title:Atherosclerosis
pub_type: 杂志文章,多中心研究
doi:10.1016/j.atherosclerosis.2019.12.014
更新日期:2020-02-01 00:00:00
abstract::Increased aortic and liver prolyl hydroxylase activity has been suggested as an early biochemical indicator of the fibrotic changes which occur in rabbits with injury induced arteriosclerosis. Daily administration of epinephrine (0.025-0.050 mg/kg, i.v.) and thyroxine (0.050 mg/kg, i.p.) to rabbits for 3 weeks produce...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(76)90140-4
更新日期:1976-09-01 00:00:00
abstract:BACKGROUND AND AIMS:An environment-wide association study (EWAS) may be useful to comprehensively test and validate associations between environmental factors and peripheral arterial disease (PAD) in an unbiased manner. METHODS:Data from cross-sectional cohorts from the US National Health and Nutrition Examination Sur...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2018.01.006
更新日期:2018-02-01 00:00:00
abstract:BACKGROUND AND AIMS:We aimed at developing a novel risk prediction model for death from cardiovascular disease (CVD) for Chinese individuals, based upon a large cohort from Taiwan. METHODS:This Chinese cohort came from Taiwan, with 381,963 individuals free from CVD, recruited from a private health surveillance program...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2017.07.016
更新日期:2017-09-01 00:00:00
abstract::Recently, we showed that connexin37 (Cx37) protects against early atherosclerotic lesion development by regulating monocyte adhesion. The expression of this gap junction protein is altered in mouse and human atherosclerotic lesions; it is increased in macrophages newly recruited to the lesions and disappears from the ...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2009.02.020
更新日期:2009-09-01 00:00:00
abstract:BACKGROUND AND AIMS:We recently showed that miR-223-3p on high-density lipoproteins (HDL) is exported to endothelial cells, where it inhibits inflammation. However, the origin of miR-223-3p on HDL is unknown. We hypothesize that HDL-associated miR-223-3p originates in myeloid cells and is exported to HDL in a scavenger...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2019.04.227
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVE:Peritoneal macrophages are used in many studies related to atherosclerosis. In situ, they are non-adherent and upon culturing, they adhere and function as scavengers of modified lipoproteins and dead apoptotic cells. They also produce growth factors, suggesting that they may provide life-supporting function a...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2011.09.014
更新日期:2011-12-01 00:00:00
abstract::To evaluate the influence of lifestyle modification with and without lipid-lowering drug therapy on the carotid arterial wall, we did a 2 year prospective ultrasound study of carotid intima-media thickness (IMT) in 1390 male and female residents of a suburban Japanese town. According to total cholesterol (TC) level at...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2003.12.025
更新日期:2004-04-01 00:00:00
abstract:BACKGROUND AND AIMS:Cell adhesion molecules (CAMs) are implicated in the initiation and progression of atherosclerosis, but their association with risk of type 2 diabetes remains inconsistent. This meta-analysis aimed to quantify this association with dose-response analysis in the general population without type 2 diab...
journal_title:Atherosclerosis
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.atherosclerosis.2019.06.908
更新日期:2019-08-01 00:00:00
abstract::Cardiovascular risk is increased in patients with diabetic nephropathy. The aim of this study was to examine the relative impacts of albuminuria and renal failure, the two important features of diabetic nephropathy, on potentially atherogenic lipoprotein changes in this condition. The subjects were 160 non-diabetic he...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(00)00673-0
更新日期:2001-06-01 00:00:00
abstract::Several reports have suggested an association between lipid peroxidation and human carotid atherosclerosis, but few reports have demonstrated a link between lipid peroxidation and carotid plaques in humans. In this study, we investigated the relationship between clinical features, histopathological characteristics and...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(01)00583-4
更新日期:2002-02-01 00:00:00
abstract:BACKGROUND AND AIMS:Several risk prediction models for coronary heart disease (CHD) are available today, however, they only explain a modest proportion of the incidence. Circulating microRNAs (miRs) have recently been associated with processes in CHD development, and may therefore represent new potential risk markers. ...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2019.07.024
更新日期:2019-10-01 00:00:00
abstract::The Framingham risk score (FRS), developed in a white cohort aged 30-74 years, is increasingly used in the early risk identification for coronary artery disease (CAD). This study examines the relationship between FRS and carotid artery intima-media thickness (IMT), a surrogate marker of coronary atherosclerosis, in bl...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(03)00244-2
更新日期:2003-09-01 00:00:00
abstract::Lipid and apoprotein composition of four very low density lipoprotein (VLDL) subfractions decreasing in Sf value were evaluated in the fasting state in 12 normolipidemic Pima Indians (6 M, 6 F, age 39 +/- 1.7 yrs) (mean +/- SEM) with non-insulin-dependent diabetes mellitus (NIDDM) in poor glycemic control (HbA1 9.8 +/...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(91)90183-4
更新日期:1991-11-01 00:00:00
abstract::In monocyte-derived macrophages from both normal and familial hypercholesterolaemic (FH) subjects, degradation of low density lipoprotein (LDL) through non-saturable pathways produced the same fall in 3-hydroxy-3-methylglutaryl-CoA reductase activity as receptor-mediated degradation of acetylated LDL, yet did not lead...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(87)90238-3
更新日期:1987-04-01 00:00:00
abstract::The prevalence of coronary heart disease (CHD), cardiovascular disease (CVD) and associated risk factors was studied in 413 men aged 70-89, the survivors of the Finnish cohorts of the Seven Countries Study. Men were divided into five categories according to manifestations of prevalent CVD: I, history or ECG evidence o...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(93)90175-t
更新日期:1993-12-01 00:00:00
abstract:AIMS:Transition metal ions have been implicated in atherosclerosis. The goal of this study was to investigate whether metal ion levels were higher in people with diabetes, in view of their increased risk of aggravated atherosclerosis. METHODS AND RESULTS:Absolute concentrations of iron, copper, zinc and calcium, and p...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2012.03.015
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVE:Elevated leukocyte count is a classic marker of systemic inflammation. This study examined whether the leukocyte count is associated with incidence of coronary events (CE) and stroke during a long follow-up period. METHODS:A total of 17,131 men and 2932 women, aged 27-61 years, without history of cardiovascu...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2009.09.029
更新日期:2010-04-01 00:00:00
abstract::No evidence of premature vascular disease is found in apolipoprotein A-I(Milano) (apoA-I(M)) human carriers, despite very low high density lipoprotein (HDL) cholesterol levels. Whether apoA-I(M) may impart a "gain of function" in atherosclerosis protection compared to wild-type apoA-I is hotly debated. To address this...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2005.03.008
更新日期:2005-12-01 00:00:00
abstract::The association between serum selenium concentration and a number of coronary heart disease risk factors is studied in 364 males from southern Italy participating in the Olivetti Heart Study. Selenium correlates positively and significantly with serum cholesterol (r = 0.120; P = 0.022), and this positive association p...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(91)90015-u
更新日期:1991-04-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of losartan-an angiotensin II type 1 receptor (AT1R) antagonist- and telmisartan-an AT1R blocker with insulin-sensitizing properties-, on the hepatic expression of plasminogen activator inhibitor-1 (PAI-1) in a rat model of nonalcoholic fatty liver disease (NAFLD). METHODS:Rats were gi...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2009.01.026
更新日期:2009-09-01 00:00:00
abstract::HDL-increasing drugs such as fenofibrate and niacin have failed to decrease the cardiovascular risk in patients with type 2 diabetes. Drug-mediated quantitative and qualitative HDL modifications could be involved in these negative results. To evaluate the quantitative and qualitative effects of niacin and fenofibrate ...
journal_title:Atherosclerosis
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.atherosclerosis.2014.12.006
更新日期:2015-02-01 00:00:00
abstract:AIMS:This cross-sectional study tested the hypothesis that treatment with the combination of Ezetimibe/Simvastatin (Vytorin) leads to broader changes in the expression levels of immunomodulatory genes as compared to Simvastatin monotherapy. METHODS:Illumina's GenomeStudio gene expression module was used to compare gen...
journal_title:Atherosclerosis
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.atherosclerosis.2013.09.031
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVES:This study examined whether combined ultrasound assessment of plaque size and echolucency in the carotid artery had an additive effect for predicting coronary events in patients with coronary artery disease (CAD). Ultrasound assessment of either plaque size or echolucency of carotid artery provides prognosti...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2010.03.003
更新日期:2010-08-01 00:00:00