Identification of intimal subendothelial cells from human aorta in primary culture.

Abstract:

:Subendothelial cells (SEC) were obtained from the inner intimal layer of adult human aorta by collagenase treatment. SEC were identified in primary culture either as smooth muscle cells by staining with FITC-labeled antisera against human smooth muscle myosin or as macrophages, foam cells and contaminating endothelial cells by their uptake of malondialdehyde treated low density lipoproteins labeled with fluorescent dye 3,3'-dioctadecylindocarbocyanine. Between 1 and 5 days in culture, along with smooth muscle cells (SMC, 38-82%), endothelial cells (0-9%), macrophages and foam cells (2-32%), one more type of cell was found. This cell type resembled SMC in size and shape, but was not stained by antisera to SMC myosin. By ultrastructural criteria these cells were characterized as modulated SMC for they contained prominent rough endoplastic reticulum and Golgi complex together with basement membrane and a large number of plasmalemmal vesicles. Like SMC they reacted with phalloidin and were stained by anti-vimentin but not by anti-desmin monoclonal antibodies. The proportion of such cells varied from 5 to 33% of total cell number and increased in parallel to macrophages and foam cells in vessels with well developed atherosclerotic lesions. We conclude that the applied technique may be used for identification of cultured vascular cells including modulated SMC.

journal_name

Atherosclerosis

journal_title

Atherosclerosis

authors

Babaev VR,Antonov AS,Zacharova OS,Romanov YA,Krushinsky AV,Tsibulsky VP,Shirinsky VP,Repin VS,Smirnov VN

doi

10.1016/0021-9150(88)90301-2

subject

Has Abstract

pub_date

1988-05-01 00:00:00

pages

45-56

issue

1

eissn

0021-9150

issn

1879-1484

pii

0021-9150(88)90301-2

journal_volume

71

pub_type

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