An update on the routine application of MALDI-TOF MS in clinical microbiology.

abstract：INTRODUCTION:Unwanted platelet activation is associated with numerous diseases, mainly thrombosis-related. In this context, proteomics has emerged as a novel tool with potential for drug target discovery and to scrutinize the effects of antiplatelet drugs. Areas covered: The present review presents the main findings of...

journal_title：Expert review of proteomics

authors： Izquierdo I,García Á

更新日期：2016-11-01 00:00:00

abstract：INTRODUCTION:Mass spectrometry (MS) is widely used in the characterization of biomolecules including peptide and protein therapeutics. These biotechnology products have seen rapid growth over the past few decades and continue to dominate the global pharmaceutical market. Advances in MS instrumentation and techniques ha...

journal_title：Expert review of proteomics

authors： Rathore D,Faustino A,Schiel J,Pang E,Boyne M,Rogstad S

更新日期：2018-05-01 00:00:00

abstract：:Protein phosphorylation events are key regulators of cellular signaling processes. In the era of functional genomics, rational drug design programs demand large-scale high-throughput analysis of signal transduction cascades. Significant improvements in the area of mass spectrometry-based proteomics have provided excit...

journal_title：Expert review of proteomics

authors： Chen WG,White FM

更新日期：2004-10-01 00:00:00

abstract：INTRODUCTION:Cutaneous leishmaniasis (CL), caused by different Leishmania parasite species, is associated with parasite-induced immune-mediated skin inflammation and ulceration. Whereas many CL studies focus on gene expression signatures in mouse models, the transcriptional response driving human patients in the field ...

journal_title：Expert review of proteomics

authors： Masoudzadeh N,Mizbani A,Rafati S

更新日期：2020-01-01 00:00:00

abstract：:This review describes a new type of label-free optical biosensor that is inexpensively manufactured from continuous sheets of plastic film and incorporated into standard format microplates to enable highly sensitive, high-throughput detection of small molecules, proteins and cells. The biosensor and associated detecti...

journal_title：Expert review of proteomics

authors： Cunningham BT,Laing L

更新日期：2006-06-01 00:00:00

abstract：:Single-cell analysis is gaining popularity in the field of mass spectrometry as a method for analyzing protein and peptide content in cells. The spatial resolution of MALDI mass spectrometry (MS) imaging is by a large extent limited by the laser focal diameter and the displacement of analytes during matrix deposition....

journal_title：Expert review of proteomics

authors： Boggio KJ,Obasuyi E,Sugino K,Nelson SB,Agar NY,Agar JN

更新日期：2011-10-01 00:00:00

abstract：INTRODUCTION:Breast cancer subtypes are currently defined by a combination of morphologic, genomic, and proteomic characteristics. These subtypes provide a molecular portrait of the tumor that aids diagnosis, prognosis, and treatment escalation/de-escalation options. Gene expression signatures describing intrinsic brea...

journal_title：Expert review of proteomics

authors： Mueller C,Haymond A,Davis JB,Williams A,Espina V

更新日期：2018-02-01 00:00:00

abstract：INTRODUCTION:The application of new proteomics methods may help to identify new diagnostic/predictive molecular markers in an attempt to improve the clinical management of atherosclerosis. Areas covered: Technological advances in proteomics have enhanced its sensitivity and multiplexing capacity, as well as the possibi...

journal_title：Expert review of proteomics

authors： Mourino-Alvarez L,Baldan-Martin M,Rincon R,Martin-Rojas T,Corbacho-Alonso N,Sastre-Oliva T,Barderas MG

更新日期：2017-08-01 00:00:00

abstract：INTRODUCTION:Lung cancer and related diseases have been one of the most common causes of deaths worldwide. Genomic-based biomarkers may hardly reflect the underlying dynamic molecular mechanism of functional protein interactions, which is the center of a disease. Recent developments in mass spectrometry (MS) have made ...

journal_title：Expert review of proteomics

authors： Fujii K,Nakamura H,Nishimura T

更新日期：2017-04-01 00:00:00

abstract：:Tremendous efforts have been put together over the last several years to complete the entire sequencing of the human genome. As we enter the proteomic era, when the major aim is understanding which gene encodes which protein, the time has also come to identify their precise function inside the astonishing signaling ne...

journal_title：Expert review of proteomics

authors： Vigneault F,Guérin SL

更新日期：2005-10-01 00:00:00

abstract：:In recent years, MALDI imaging mass spectrometry (MALDI-IMS) has developed as a promising tool to investigate the spatial distribution of biomolecules in intact tissue specimens. Ion densities of various molecules can be displayed as heat maps while preserving anatomical structures. In this short review, an overview o...

journal_title：Expert review of proteomics

authors： Chatterji B,Pich A

更新日期：2013-08-01 00:00:00

abstract：:It is well known that chronic, excessive consumption of alcohol can cause brain damage/structural changes in the regions important for neurocognitive function. Some of the damages are permanent, while others are reversible. Molecular mechanisms underlying alcohol-induced and/or -related brain damage are largely unknow...

journal_title：Expert review of proteomics

authors： Matsumoto H,Matsumoto I

更新日期：2008-04-01 00:00:00

abstract：:Elucidating disulfide linkage patterns is a crucial part of protein characterization, for which mass spectrometry (MS) is now an indispensable analytical tool. In many cases, MS-based disulfide connectivity assignment is straightforwardly achieved using one-step protein fragmentation in the unreduced form followed by ...

journal_title：Expert review of proteomics

authors： Goyder MS,Rebeaud F,Pfeifer ME,Kálmán F

更新日期：2013-10-01 00:00:00

abstract：INTRODUCTION:Mass spectrometry (MS)-based proteomics has become an indispensable tool for the characterization of the proteome and its post-translational modifications (PTM). In addition to standard protein sequence databases, proteogenomics strategies search the spectral data against the theoretical spectra obtained f...

journal_title：Expert review of proteomics

authors： Hernandez-Valladares M,Vaudel M,Selheim F,Berven F,Bruserud Ø

更新日期：2017-08-01 00:00:00

abstract：:Proteins have several measurable features in biological fluids that may change under pathological conditions. The current disease biomarker discovery is mostly based on protein concentration in the sample as the measurable feature. Changes in protein structures, such as post-translational modifications and in protein-...

journal_title：Expert review of proteomics

authors： Zaslavsky BY,Uversky VN,Chait A

更新日期：2016-01-01 00:00:00

abstract：:Clinical proteomics has been applied to the identification of biomarkers of obstetric and neonatal disease. We will discuss a number of encouraging studies that have led to potentially valid biomarkers in the context of Down's syndrome, preterm birth, amniotic infections, preeclampsia, intrauterine growth restriction ...

journal_title：Expert review of proteomics

authors： Klein J,Buffin-Meyer B,Mullen W,Carty DM,Delles C,Vlahou A,Mischak H,Decramer S,Bascands JL,Schanstra JP

更新日期：2014-02-01 00:00:00

abstract：:An essential skill for every researcher is to learn how to select and apply the most appropriate methods for the questions they are trying to answer. With the extensive variety of methods available, it is increasingly important to scrutinize the advantages and disadvantages of these techniques prior to making a decisi...

journal_title：Expert review of proteomics

authors： Weibrecht I,Leuchowius KJ,Clausson CM,Conze T,Jarvius M,Howell WM,Kamali-Moghaddam M,Söderberg O

更新日期：2010-06-01 00:00:00

abstract：:Ovarian cancer is the fifth leading cause of cancer deaths among North American women. Regrettably, there is currently no reliable circulating biomarker that can detect ovarian cancer in its early stages. The CA125 biomarker is very useful for treatment response monitoring, but its sensitivity is very low for early de...

journal_title：Expert review of proteomics

authors： Ye B,Gagnon A,Mok SC

更新日期：2007-02-01 00:00:00

abstract：:Mammalian sperm are differentiated germ cells that transfer genetic material from the male to the female. Owing to this essential role in the reproductive process, an understanding of the complex mechanisms that underlie sperm function has implications ranging from the development of novel contraceptives to the treatm...

journal_title：Expert review of proteomics

authors： Porambo JR,Salicioni AM,Visconti PE,Platt MD

更新日期：2012-10-01 00:00:00

abstract：:Antigen processing forwards various information about the cellular status and the proteome to the cell surface for scrutiny by the cellular immune system. Thus the repertoire of major histocompatibility complex (MHC)-bound peptides and the MHC ligandome, indirectly mirrors the proteome in order to make alterations ins...

journal_title：Expert review of proteomics

authors： Hillen N,Stevanovic S

更新日期：2006-12-01 00:00:00

abstract：:Huntington's disease (HD) is caused by expansion of a polyglutamine repeat in the N-terminal region of huntingtin (htt), a large protein that has been found to interact with a variety of proteins. It remains to be determined how the interactions of htt with other proteins are involved in the pathogenesis of HD. A rece...

journal_title：Expert review of proteomics

authors： Li S,Li XJ

更新日期：2012-01-01 00:00:00

abstract：:Evaluation of: Di Girolamo F, Boschetti E, Chung MC, Guadagni F, Righetti PG. 'Proteomineering' or not? The debate on biomarker discovery in sera continues. J. Proteomics 74(5), 589-594 (2011). The combinatorial peptide ligand library in association with mass spectrometry can greatly enhance the dynamic range of the a...

journal_title：Expert review of proteomics

authors： Panfoli I,Bruschi M,Ravera S,Candiano G

更新日期：2011-08-01 00:00:00

abstract：INTRODUCTION:The human respiratory system is highly prone to diseases and complications. Many lung diseases, including lung cancer (LC), tuberculosis (TB), and chronic obstructive pulmonary disease (COPD) have been among the most common causes of death worldwide. Cystic fibrosis (CF), the most common genetic disease in...

journal_title：Expert review of proteomics

authors： Tan HW,Xu YM,Wu DD,Lau ATY

更新日期：2018-02-01 00:00:00

abstract：:Neurodegenerative diseases are devastating disorders and the demands on their treatment are set to rise in connection with higher disease incidence. Knowledge of the spatiotemporal profile of cellular protein expression during neural differentiation and definition of a set of markers highly specific for targeted neura...

journal_title：Expert review of proteomics

authors： Zizkova M,Sucha R,Tyleckova J,Jarkovska K,Mairychova K,Kotrcova E,Marsala M,Gadher SJ,Kovarova H

更新日期：2015-02-01 00:00:00

abstract：:In spite of the effective chronic myeloid leukemia (CML) therapy, a small percentage will fail on therapy and develop acute myeloid leukemia-like blast crisis. Understanding the underlying biology of therapy resistance is probably needed to develop better blood cancer therapy, and CML may be an ideal disease model for...

journal_title：Expert review of proteomics

authors： Gjertsen BT,Wiig H

更新日期：2012-12-01 00:00:00

abstract：:Organellar proteomics aims to describe the full complement of proteins of subcellular structures and organelles. When compared with whole-cell or whole-tissue proteomes, the more focused results from subcellular proteomic studies have yielded relatively simpler datasets from which biologically relevant information can...

journal_title：Expert review of proteomics

authors： Gauthier DJ,Lazure C

更新日期：2008-08-01 00:00:00

abstract：:A major goal of proteomics is to develop methods that enable the systematic characterization of every protein within the cell or particular subcellular proteome using a single analytical platform. Although the equivalent has already been achieved in genomics, reaching this goal in proteomics represents a much greater ...

journal_title：Expert review of proteomics

authors： Blonder J,Conrads TP,Veenstra TD

更新日期：2004-08-01 00:00:00

abstract：:Proteomics is performed in microgravity research in order to determine protein alterations occurring qualitatively and quantitatively, when single cells or whole organisms are exposed to real or simulated microgravity. To this purpose, antibody-dependent (Western blotting, flow cytometry, Luminex(®) technology) and an...

journal_title：Expert review of proteomics

authors： Grimm D,Pietsch J,Wehland M,Richter P,Strauch SM,Lebert M,Magnusson NE,Wise P,Bauer J

更新日期：2014-08-01 00:00:00

abstract：:The vascular endothelium lining the luminal surface of all blood vessels is constantly exposed to shear stress exerted by the flowing blood. Blood flow with high laminar shear stress confers protection by activation of antiatherogenic, antithrombotic and anti-inflammatory proteins, whereas low or oscillatory shear str...

journal_title：Expert review of proteomics

authors： Firasat S,Hecker M,Binder L,Asif AR

更新日期：2014-10-01 00:00:00

abstract：:Type II diabetes increases the risk for cognitive decline via multiple traits. Amylin is a pancreatic hormone that has amyloidogenic and cytotoxic properties similar to the amyloid-β peptide. The amylin hormone is overexpressed in individuals with pre-diabetic insulin resistance or obesity leading to amylin oligomeriz...

journal_title：Expert review of proteomics

authors： Ly H,Despa F

更新日期：2015-01-01 00:00:00