An update on the routine application of MALDI-TOF MS in clinical microbiology.


:Introduction: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has entered clinical diagnostics and is today a generally accepted and integral part of the workflow for microbial identification. MALDI-TOF MS identification systems received approval from national and international institutions, such as the USA-FDA, and are continuously improved and adopted to other fields like veterinary and industrial microbiology. The question is whether MALDI-TOF MS also has the potential to replace other conventional and molecular techniques operated in routine diagnostic laboratories. Areas covered: We give an overview of new advancements of mass spectral analysis in the context of microbial diagnostics. In particular, the expansion of databases to increase the range of readily identifiable bacteria and fungi, the refined discrimination of species complexes, subspecies, and types, the testing for antibiotic resistance or susceptibility, progress in sample preparation including automation, and applications of other mass spectrometry techniques are discussed. Expert opinion: Although many new approaches of MALDI-TOF MS are still in the stage of proof of principle, it is expectable that MALDI-TOF MS will expand its role in the clinical microbiology laboratory of the future. New databases, instruments and analytical software modules will continue to be developed to further improve diagnostic efficacy.


Expert Rev Proteomics


Welker M,Van Belkum A,Girard V,Charrier JP,Pincus D




Has Abstract


2019-08-01 00:00:00












  • Urinary biomarkers of physical activity: candidates and clinical utility.

    abstract::Chronic physical inactivity is a major risk factor for a number of important lifestyle diseases, while inappropriate exposure to high physical demands is a risk factor for musculoskeletal injury and fatigue. Proteomic and metabolomic investigations of the physical activity continuum - extreme sedentariness to extremes...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Sampson DL,Broadbent JA,Parker AW,Upton Z,Parker TJ

    更新日期:2014-02-01 00:00:00

  • Investigation of therapy resistance mechanisms in myeloid leukemia by protein profiling of bone marrow extracellular fluid.

    abstract::In spite of the effective chronic myeloid leukemia (CML) therapy, a small percentage will fail on therapy and develop acute myeloid leukemia-like blast crisis. Understanding the underlying biology of therapy resistance is probably needed to develop better blood cancer therapy, and CML may be an ideal disease model for...

    journal_title:Expert review of proteomics

    pub_type: 评论,杂志文章


    authors: Gjertsen BT,Wiig H

    更新日期:2012-12-01 00:00:00

  • Proteomic profiling of fatty acid binding proteins in muscular dystrophy.

    abstract::Introduction: Duchenne muscular dystrophy is a neuromuscular disorder, which is caused by abnormalities in the DMD gene that encodes the membrane cytoskeletal protein dystrophin. Besides progressive skeletal muscle wasting, dystrophinopathy also affects non-skeletal muscle tissues, including cells in the cardio-respir...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Dowling P,Gargan S,Zweyer M,Swandulla D,Ohlendieck K

    更新日期:2020-02-01 00:00:00

  • A decade of proteomics accomplished! Central and Eastern European Proteomic Conference (CEEPC) celebrates its 10th Anniversary in Budapest, Hungary.

    abstract::The Central and Eastern European Proteomic Conference (CEEPC) proudly celebrated its 10th Anniversary with an exciting scientific program inclusive of proteome, proteomics and systems biology in Budapest, Hungary. Since 2007, CEEPC has represented 'state-of the-art' proteomics in and around Central and Eastern Europe ...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Gadher SJ,Drahos L,Vékey K,Kovarova H

    更新日期:2017-07-01 00:00:00

  • Novel methods in glycomics: a 2019 update.

    abstract::Introduction: Glycomics, which aims to define the glycome of a biological system to better assess the biological attributes of the glycans, has attracted increasing interest. However, the complexity and diversity of glycans present challenging barriers to glycome definition. Technological advances are major drivers in...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Cao WQ,Liu MQ,Kong SY,Wu MX,Huang ZZ,Yang PY

    更新日期:2020-01-01 00:00:00

  • Understanding type 1 diabetes through proteomics.

    abstract:INTRODUCTION:Auto-immunity against pancreatic beta-cells leads to an absolute shortage of the hormone insulin, resulting in hyperglycemia and the onset of type 1 diabetes (T1D). Proteomic approaches have been used to elucidate the mechanisms of beta-cell dysfunction and death. Areas covered: In the present review, we d...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Crèvecoeur I,Vig S,Mathieu C,Overbergh L

    更新日期:2017-07-01 00:00:00

  • Recent advances in proteomic profiling of pancreatic ductal adenocarcinoma and the road ahead.

    abstract:INTRODUCTION:Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers worldwide. However, there remain many unmet clinical needs, from diagnosis to treatment strategies. The inherent complexity of the molecular characteristics of PDAC has made it difficult to meet these challenges, rendering proteomic pr...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Kim H,Park J,Wang JI,Kim Y

    更新日期:2017-11-01 00:00:00

  • The role of proteomics in assessing beta-cell dysfunction and death in type 1 diabetes.

    abstract::Introduction: Type 1 diabetes (T1D) is characterized by autoimmune-induced dysfunction and destruction of the pancreatic beta cells. Unfortunately, this process is poorly understood, and the current best treatment for type 1 diabetes is the administration of exogenous insulin. To better understand these mechanisms and...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Nakayasu ES,Qian WJ,Evans-Molina C,Mirmira RG,Eizirik DL,Metz TO

    更新日期:2019-07-01 00:00:00

  • Shining a light on defective autophagy by proteomics approaches: implications for neurodegenerative illnesses.

    abstract::Introduction: Autophagy is one of the most conserved clearance systems through which eukaryotes manage to handle dysfunctional and excess organelles and macromolecules. This catabolic process has not only a role in the maintenance of basal turnover of cellular components, but it is also essential in cells adaptation t...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Di Domenico F,Zuliani I,Tramutola A

    更新日期:2019-01-01 00:00:00

  • Human saliva proteome analysis and disease biomarker discovery.

    abstract::Human saliva is an attractive body fluid for disease diagnosis and prognosis because saliva testing is simple, safe, low-cost and noninvasive. Comprehensive analysis and identification of the proteomic content in human whole and ductal saliva will not only contribute to the understanding of oral health and disease pat...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Hu S,Loo JA,Wong DT

    更新日期:2007-08-01 00:00:00

  • Proteomics advances for precision therapy in ovarian cancer.

    abstract::Introduction: Due to the relatively low mutation rate and high frequency of copy number variation, finding actionable genetic drivers of high-grade serous carcinoma (HGSC) is a challenging task. Furthermore, emerging studies show that genetic alterations are frequently poorly represented at the protein level adding a ...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Labrie M,Kendsersky ND,Ma H,Campbell L,Eng J,Chin K,Mills GB

    更新日期:2019-10-01 00:00:00

  • Tissue microarrays: bridging the gap between research and the clinic.

    abstract::Tissue microarrays are a high-throughput method for the investigation of biomarkers in multiple tissue specimens at once. This technique allows for the analysis of up to 500 tissue samples in a single experiment using immunohistochemistry and in situ hybridization. Recently, cell lines and xenografts have been reduced...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Braunschweig T,Chung JY,Hewitt SM

    更新日期:2005-06-01 00:00:00

  • Contribution of mass spectrometry-based proteomics to immunology.

    abstract::Antigen processing forwards various information about the cellular status and the proteome to the cell surface for scrutiny by the cellular immune system. Thus the repertoire of major histocompatibility complex (MHC)-bound peptides and the MHC ligandome, indirectly mirrors the proteome in order to make alterations ins...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Hillen N,Stevanovic S

    更新日期:2006-12-01 00:00:00

  • Concepts and strategies of soybean seed proteomics using the shotgun proteomics approach.

    abstract::Introduction: The last decade has yielded significant developments in the field of proteomics, especially in mass spectrometry (MS) and data analysis tools. In particular, a shift from gel-based to MS-based proteomics has been observed, thereby providing a platform with which to construct proteome atlases for all life...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Min CW,Gupta R,Agrawal GK,Rakwal R,Kim ST

    更新日期:2019-09-01 00:00:00

  • Advanced technologies for studying circulating tumor cells at the protein level.

    abstract::Metastasis is the main cause of cancer death. As the tumor progresses, cells from the primary tumor site are shed into the bloodstream as circulating tumor cells (CTCs). Eventually, these cells colonize other organs and form distant metastases. It is therefore imperative that we gain a better understanding of the biol...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Chan CM,Au TC,Chan AT,Ma BB,Tsui NB,Ng SS,Hui EP,Chan LW,Ho WS,Yung BY,Wong SC

    更新日期:2013-12-01 00:00:00

  • Proteomics: advances in biomarker discovery.

    abstract::The challenges encountered by proteomic researchers seeking diagnostic, prognostic and mechanistic markers were the subject of the 1-day meeting, Proteomics: Advances in Biomarker Discovery hosted by EuroSciCon. The speakers had a broad range of clinical and basic science interests, and presented data using a number o...

    journal_title:Expert review of proteomics



    authors: Cowan ML,Vera J

    更新日期:2008-02-01 00:00:00

  • Proteomic studies of common chronic pain conditions - a systematic review and associated network analyses.

    abstract:INTRODUCTION:The lack of biomarkers indicating involved nociceptive and/or pain mechanisms makes diagnostic procedures problematic. Clinical pain research has begun to use proteomics. AREAS COVERED:This systematic review covers proteomic studies of chronic pain cohorts and in relation to clinical variables. Searches i...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Gerdle B,Ghafouri B

    更新日期:2020-06-01 00:00:00

  • Top-down Proteomics: Technology Advancements and Applications to Heart Diseases.

    abstract:INTRODUCTION:Heart diseases are a leading cause of morbidity and mortality for both men and women worldwide, and impose significant economic burdens on the healthcare systems. Despite substantial effort over the last several decades, the molecular mechanisms underlying diseases of the heart remain poorly understood. A...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Cai W,Tucholski TM,Gregorich ZR,Ge Y

    更新日期:2016-08-01 00:00:00

  • Detecting peptidic drugs, drug candidates and analogs in sports doping: current status and future directions.

    abstract::With the growing availability of mature systems and strategies in biotechnology and the continuously expanding knowledge of cellular processes and involved biomolecules, human sports drug testing has become a considerably complex field in the arena of analytical chemistry. Proving the exogenous origin of peptidic drug...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Thevis M,Thomas A,Schänzer W

    更新日期:2014-12-01 00:00:00

  • Recent mass spectrometry-based proteomics for biomarker discovery in lung cancer, COPD, and asthma.

    abstract:INTRODUCTION:Lung cancer and related diseases have been one of the most common causes of deaths worldwide. Genomic-based biomarkers may hardly reflect the underlying dynamic molecular mechanism of functional protein interactions, which is the center of a disease. Recent developments in mass spectrometry (MS) have made ...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Fujii K,Nakamura H,Nishimura T

    更新日期:2017-04-01 00:00:00

  • Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone.

    abstract:OBJECTIVE:Hepcidin-25 production is stimulated by systemic inflammation, and it interferes with iron utilization, leading to anemia. This study aimed to investigate the relationships between the plasma levels of hepcidin, interleukin-6 (IL-6), erythropoietin (EPO) and erythroferrone (ERFE) in patients with benign breas...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Ciniselli CM,De Bortoli M,Taverna E,Varinelli L,Pizzamiglio S,Veneroni S,Bonini C,Orlandi R,Verderio P,Bongarzone I

    更新日期:2015-01-01 00:00:00

  • Wisteria floribunda agglutinin positive glycobiomarkers: a unique lectin as a serum biomarker probe in various diseases.

    abstract:INTRODUCTION:Serum proteins are generally glycosylated and solubilized, and are thus present as glycoproteins. The glycan structure of glycoproteins reflects cell differentiation status; glycan structures generated by diseased cells are distinguishable from those produced by healthy cells. Proteins may therefore serve ...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Narimatsu H,Sato T

    更新日期:2018-02-01 00:00:00

  • Advances in endothelial shear stress proteomics.

    abstract::The vascular endothelium lining the luminal surface of all blood vessels is constantly exposed to shear stress exerted by the flowing blood. Blood flow with high laminar shear stress confers protection by activation of antiatherogenic, antithrombotic and anti-inflammatory proteins, whereas low or oscillatory shear str...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Firasat S,Hecker M,Binder L,Asif AR

    更新日期:2014-10-01 00:00:00

  • Complementary methods to assist subcellular fractionation in organellar proteomics.

    abstract::Organellar proteomics aims to describe the full complement of proteins of subcellular structures and organelles. When compared with whole-cell or whole-tissue proteomes, the more focused results from subcellular proteomic studies have yielded relatively simpler datasets from which biologically relevant information can...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Gauthier DJ,Lazure C

    更新日期:2008-08-01 00:00:00

  • Applying selected reaction monitoring to targeted proteomics.

    abstract::Selected reaction monitoring (SRM) is a highly selective and sensitive mass spectrometric methodology for precise and accurate quantification of low-abundant proteins in complex mixtures and for characterization of modified peptides, and constitutes the method of choice in targeted proteomics. Owing to its outstanding...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Calvo E,Camafeita E,Fernández-Gutiérrez B,López JA

    更新日期:2011-04-01 00:00:00

  • Proteome-wide analysis of neural stem cell differentiation to facilitate transition to cell replacement therapies.

    abstract::Neurodegenerative diseases are devastating disorders and the demands on their treatment are set to rise in connection with higher disease incidence. Knowledge of the spatiotemporal profile of cellular protein expression during neural differentiation and definition of a set of markers highly specific for targeted neura...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Zizkova M,Sucha R,Tyleckova J,Jarkovska K,Mairychova K,Kotrcova E,Marsala M,Gadher SJ,Kovarova H

    更新日期:2015-02-01 00:00:00

  • Regulation of gene expression: probing DNA-protein interactions in vivo and in vitro.

    abstract::Tremendous efforts have been put together over the last several years to complete the entire sequencing of the human genome. As we enter the proteomic era, when the major aim is understanding which gene encodes which protein, the time has also come to identify their precise function inside the astonishing signaling ne...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Vigneault F,Guérin SL

    更新日期:2005-10-01 00:00:00

  • Advances in the development of human protein microarrays.

    abstract:INTRODUCTION:High-content protein microarrays in principle enable the functional interrogation of the human proteome in a broad range of applications, including biomarker discovery, profiling of immune responses, identification of enzyme substrates, and quantifying protein-small molecule, protein-protein and protein-DN...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Duarte JG,Blackburn JM

    更新日期:2017-07-01 00:00:00

  • Medical implications of understanding the functions of human small heat shock proteins.

    abstract::Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that are implicated in a variety of diseases. Upon stress, they stabilize unfolding proteins and prevent them from aggregating. However, under physiological conditions without severe stress, some sHsps interact with other proteins. In a perspective ...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Mymrikov EV,Haslbeck M

    更新日期:2015-06-01 00:00:00

  • Proximity ligation assays: a recent addition to the proteomics toolbox.

    abstract::An essential skill for every researcher is to learn how to select and apply the most appropriate methods for the questions they are trying to answer. With the extensive variety of methods available, it is increasingly important to scrutinize the advantages and disadvantages of these techniques prior to making a decisi...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Weibrecht I,Leuchowius KJ,Clausson CM,Conze T,Jarvius M,Howell WM,Kamali-Moghaddam M,Söderberg O

    更新日期:2010-06-01 00:00:00