Abstract:
:Two-photon (2P) microscopy is a powerful tool for imaging and exploring label-free biological tissues at high resolution. Although this type of microscopy has been demonstrated in ex vivo ocular tissues of both humans and animal models, imaging the human eye in vivo has always been challenging. This work presents a novel compact 2P microscope for non-contact imaging of the anterior part of the living human eye. The performance of the instrument was tested and the maximum permissible exposure to protect ocular tissues established. To the best of our knowledge, 2P images of the in vivo human cornea, the sclera and the trabecular meshwork are shown for the very first time. Acquired images are of enough quality to visualize collagen arrangement and morphological features of clinical interest. Future implementations of this technique may constitute a potential tool for early diagnosis of ocular diseases at submicron scale.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ávila FJ,Gambín A,Artal P,Bueno JMdoi
10.1038/s41598-019-46568-zsubject
Has Abstractpub_date
2019-07-12 00:00:00pages
10121issue
1issn
2045-2322pii
10.1038/s41598-019-46568-zjournal_volume
9pub_type
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