Abstract：
:A global transcriptional regulator, MgrA, was previously identified as a key determinant of virulence in Staphylococcus aureus. An 80% EtOH extract of Uncaria gambier was found to attenuate the virulence of S. aureus via its effects on MgrA. Using bioassay-guided fractionation, a polyphenolic polymer, uncariitannin, was found to be the main bioactive constituent of the extract, and its structure was characterized using spectral and chemical analysis. The molecular weight and polydispersity of uncariitannin were determined by gel permeation chromatography-refractive index-light scattering analysis. An electrophoretic mobility shift assay showed that uncariitannin could effectively inhibit the interaction of MgrA with DNA in a dose-dependent manner. Treatment with uncariitannin could decrease the mRNA and protein levels of Hla in both the S. aureus Newman and USA300 LAC strains. Further analysis of Hla expression levels in the Newman ΔmgrA and Newman ΔmgrA/pYJ335-mgrA strains indicated that uncariitannin altered Hla expression primarily in an MgrA-dependent manner. A mouse model of infection indicated that uncariitannin could attenuate MRSA virulence. In conclusion, uncariitannin may be a potential candidate for further development as an antivirulence agent for the treatment of S. aureus infection.
journal_name
Pharmacol Resjournal_title
Pharmacological researchauthors
Zhang H,Jiang JM,Han L,Lao YZ,Zheng D,Chen YY,Wan SJ,Zheng CW,Tan HS,Li ZG,Xu HXdoi
10.1016/j.phrs.2019.104328subject
Has Abstractpub_date
2019-09-01 00:00:00pages
104328eissn
1043-6618issn
1096-1186pii
S1043-6618(18)31570-6journal_volume
147pub_type
杂志文章abstract：:Cannabinoids have emerged as promising neuroprotective agents due to their capability to activate specific targets, which are involved in the control of neuronal homeostasis and survival. Specifically, those ligands that selectively target and activate the CB2 receptor may be useful for their anti-inflammatory and neu...
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更新日期：2012-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2019-11-01 00:00:00
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journal_title：Pharmacological research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
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更新日期：2019-08-01 00:00:00
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更新日期：2020-12-08 00:00:00
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更新日期：2006-10-01 00:00:00
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更新日期：2001-02-01 00:00:00
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更新日期：2011-08-01 00:00:00
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更新日期：2003-11-01 00:00:00
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更新日期：2009-09-01 00:00:00
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更新日期：2020-01-01 00:00:00
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更新日期：2018-05-01 00:00:00
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更新日期：2012-03-01 00:00:00
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pub_type： 临床试验,杂志文章
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更新日期：2001-04-01 00:00:00
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journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1006/phrs.2000.0683
更新日期：2000-09-01 00:00:00
abstract：:Plasma lipid and lipoprotein levels were evaluated in 27 adults with essential hypertension at four different periods during a 12-month treatment with doxazosin. Mean plasma total cholesterol (TC), low-density lipoprotein-cholesterol (LDLC), triglyceride (TG), very low density lipoprotein-cholesterol (VLDLC) and the L...
journal_title：Pharmacological research
pub_type： 临床试验,杂志文章
doi：10.1016/1043-6618(94)80108-8
更新日期：1994-10-01 00:00:00
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journal_title：Pharmacological research
pub_type： 杂志文章,评审
doi：10.1016/j.phrs.2019.104595
更新日期：2020-02-01 00:00:00
abstract：:The pharmacological physiological effects of the endogenous cannabinomimetic (endocannabinoid) anandamide have been well characterized. Another endocannabinoid, 2-arachidonoyl-glycerol (2-AG), has been less-widely studied. 2-AG occurs in vertebrate and invertebrate tissues and binds to both cannabinoid receptors (CB1 ...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1006/phrs.2000.0702
更新日期：2000-10-01 00:00:00
abstract：:Transdermal iontophoresis (TI) is a physical enhancement technique to facilitate the delivery of primarily charged molecules across the skin. TI of peptides is influenced by a complex interplay of several factors and one of the main issues in optimizing iontophoretic delivery of peptides is to improve the transport ef...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/s1043-6618(03)00080-x
更新日期：2003-08-01 00:00:00
