Abstract:
:Synthetic DNA is becoming an attractive substrate for digital data storage due to its density, durability, and relevance in biological research. A major challenge in making DNA data storage a reality is that reading DNA back into data using sequencing by synthesis remains a laborious, slow and expensive process. Here, we demonstrate successful decoding of 1.67 megabytes of information stored in short fragments of synthetic DNA using a portable nanopore sequencing platform. We design and validate an assembly strategy for DNA storage that drastically increases the throughput of nanopore sequencing. Importantly, this assembly strategy is generalizable to any application that requires nanopore sequencing of small DNA amplicons.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Lopez R,Chen YJ,Dumas Ang S,Yekhanin S,Makarychev K,Racz MZ,Seelig G,Strauss K,Ceze Ldoi
10.1038/s41467-019-10978-4subject
Has Abstractpub_date
2019-07-03 00:00:00pages
2933issue
1issn
2041-1723pii
10.1038/s41467-019-10978-4journal_volume
10pub_type
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