Abstract：
:In recent thirty years, protein-based nanoparticles have attracted considerable attention, and they are being widely used in the food, pharmaceutical, and biomedical fields. Wheat glutenin, an important natural vegetable protein, has been demonstrated to be nutritive and biocompatible. This study aimed to develop a new type of redox-sensitive protein nanoparticles. The glutenin nanoparticles (GNPs) were synthesized with glutenin concentrations (0.082%, 0.5%, and 0.83%) through the adoption of an antisolvent titration technique and the use of hydrogen peroxide (H2O2) oxidative cross-linking for different periods. At a glutenin concentration of 0.83% and oxidation time of 20 h, the obtained GNPs were spherical in shape and approximately 100-300 nm in size, as measured by transmission electron microscopy and dynamic light scattering. The formation of disulfide was confirmed by Raman spectroscopy. The turbidity values of the GNP suspensions were decreased by half after the addition of β-mercaptoethanol. Nile blue A, a model hydrophilic substance, was entrapped in the GNPs with 77.67% loading efficiency. The newly developed GNPs can be used as redox-responsive carriers for delivering hydrophilic active substances.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Li F,Qiu C,Li M,Xiong L,Shi Y,Sun Qdoi
10.1016/j.ijbiomac.2019.06.220subject
Has Abstractpub_date
2019-09-15 00:00:00pages
327-336eissn
0141-8130issn
1879-0003pii
S0141-8130(19)32797-7journal_volume
137pub_type
杂志文章abstract：:The present research investigates on task-specific deep eutectic solvents (TDES) based aqueous two-phase extraction and purification of immunoglobulins by chromatography from quail egg. The synthesis of TDES was accomplished with quaternary ammonium salt as hydrogen bond acceptor (HBA) and glycerol as hydrogen bond do...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.12.242
更新日期：2020-03-01 00:00:00
abstract：:Recombinant hybrid Manganese superoxide dismutase from Staphyloccus saphropyticus/S. equorum (rMnSODSeq) exhibits stability at high temperatures. The enzyme occurs as a dimer that dissociates around 52°C prior to unfolding of the monomer around 64°C, demonstrating contribution of the dimeric form to stability. Here, s...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.01.096
更新日期：2017-05-01 00:00:00
abstract：:Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability. The goal of this paper is to stabilize ZNPs using a sulfated anionic polysaccharide-dextran sulfate (DS). Zein/DS composite nanoparticles (ZDSNPs) were fabricated by antisolvent precipitation method at pH 4.0 with an optimal zein-t...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.10.149
更新日期：2020-05-15 00:00:00
abstract：:Nitric oxide (NO) is a promising therapeutic agent with antibacterial and wound-healing properties. However, the gaseous state and short half-life of NO necessitate a formulation that can control its storage and release. In this study, we developed NO-releasing films (CS/NO film) composed of chitosan (CS) and S-nitros...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.04.073
更新日期：2015-08-01 00:00:00
abstract：:Although the fungal ability to bind heavy metals has been known for many years, it was not determined which component is responsible for this process. The aim of the study was to isolate (1 → 3)-α-d-glucans from various fungi, select the most efficient compound in the biosorption of heavy metals, and determine its cha...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.07.036
更新日期：2019-09-15 00:00:00
abstract：:Effects of water activity (aW) and solvent ordering were separately analyzed on the thermal unfolding of lysozyme and alpha-chymotrypsinogen A, and also on the thermal deactivation of yeast alcohol dehydrogenase (YADH) in aqueous solutions with various additives. With the coexistence of additives, water activity was t...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(01)00127-1
更新日期：2001-06-12 00:00:00
abstract：:Alzheimer's disease (AD) is the only one among top ten diseases in USA that cannot be cured, prevented or slowed down. At molecular level the mechanism of onset has been closely associated with mis-folding of Aβ40 and Aβ42 and is well supported by the genetic data for AD. Extensive research efforts have led to identif...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.04.082
更新日期：2016-08-01 00:00:00
abstract：:The Raman spectra of kappa and iota carrageenan, a desulphated furcellaran and a series of oligomers have been compared in the region 700-1500 cm-1. Spectral differences depending on the amount and the location of the sulphate group on the ring, the chain length, the nature of the counterion and the conformation are d...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(89)90017-2
更新日期：1989-10-01 00:00:00
abstract：:Six low molecular fucoidan (DFPS) derivatives were synthesized successfully, and their potential antioxidant activities were investigated employing various established in vitro systems. All DFPS derivatives possessed considerable antioxidant activity, and had stronger antioxidant ability than DFPS in certain tests. Th...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.02.001
更新日期：2009-06-01 00:00:00
abstract：:Pleurotus ostreatus is popularly consumed as traditional medicine and health food for enhancing immune function in China. Polysaccharides from mushroom have been demonstrated to possess a wide range of health beneficial properties. This study was carried out to elucidate the immunomodulating effects and molecular mech...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.05.045
更新日期：2014-08-01 00:00:00
abstract：:To contribute towards effective exploitation and utilization of spent mushroom substrate (SMS), a water-soluble polysaccharide named PL was isolated and purified from SMS. The total sugar content and monosaccharide composition were analyzed by phenol-sulfuric acid method and capillary electrophoresis, and infrared spe...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2011.11.016
更新日期：2012-04-01 00:00:00
abstract：:Regenerated chitin (RCh)/poly (lactic acid) (PLA) blends with enhanced properties were successfully prepared via the Pickering emulsion approach. An oil-in-water Pickering emulsion of PLA/dichloromethane (DCM) stabilized with RCh via an ultrasonic homogenizer was formed. A uniformly dispersed blends were obtained upon...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.08.117
更新日期：2019-11-01 00:00:00
abstract：:In this study starches isolated from Indian quinoa varieties were examined for physicochemical, morphological, thermal and rheological properties. Among isolated starches V1 showed higher starch yield and lower purity (48.45% and 98.32%) than V2 (41.28 and 98.53%). The amylose content was higher for V1 (12.10%) than V...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.04.027
更新日期：2017-09-01 00:00:00
abstract：:In this study, the adjuvant activity of sulfated glucan from saccharomyces cerevisiae (SGSC) was investigated. sGSC had molecular weight of 12.9kDa and degrees of sulfation of 0.16. The effects of SGSC on the splenic lymphocyte cells of mice were measured in vitro. The results showed that SGSC could significantly prom...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.06.057
更新日期：2016-12-01 00:00:00
abstract：:This is the first report on the identification and partial characterization of phospholipase D (EC 3.1.4.4) from Allium sativum (garlic) bulbs (PLD(GB)). The enzyme shares the phenomenon of interfacial activation with other lipolytic enzymes, i.e. the hydrolytic rate increases when the substrate changes to a more aggr...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2008.01.007
更新日期：2008-05-01 00:00:00
abstract：:The biosynthesis of medium chain length poly(3-hydroxyalkanoates) mcl PHAs by Pseudomonas guezennei using glucose, sodium octanoate, and 10-undecenoic acid as sole or mixed carbon sources was investigated. Chemical composition of polyesters was analyzed by GCMS and NMR. The copolyester produced by P. guezennei from gl...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.08.018
更新日期：2012-12-01 00:00:00
abstract：:Gelatinization and retrogradation characteristics of starches from tigernut (Cyperus esculentus) tuber before and after various oil extraction processes were studied in this investigation. The results indicated that starches isolated from tigernut tuber after the various oil extraction processes varied significantly i...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.252
更新日期：2020-08-01 00:00:00
abstract：:In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respect...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.07.104
更新日期：2018-11-01 00:00:00
abstract：:The hot acid extract of pea hull, HSP, was rich in galacturonic acid, arabinose and xylose. It was fractionated by copper precipitation followed by ion-exchange chromatography. The copper-soluble fraction represented 26% of HSP and was mostly composed of an arabinan with a low degree of branching, some heteroxylans an...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(97)00055-x
更新日期：1997-08-01 00:00:00
abstract：:In the present study, the conformational changes of bovine submaxillary mucin (BSM) adsorbed on a hydrophobic surface (polystyrene (PS)) as a function of concentration in bulk solution (up to 2mg/mL) have been investigated with biomolecular probe-based approaches, including bicinchoninic acid (BCA), enzyme-linked immu...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.09.035
更新日期：2015-01-01 00:00:00
abstract：:Ultracentrifugation was used to clarify seven amylose fractions obtained by aqueous leaching of corn starch by 5 degrees C steps over a temperature range of 65-95 degrees C. Each fraction was characterized by its hydrodynamic radius R-(H), as determined by dynamic light scattering--and by the macromolecular features (...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(96)01101-4
更新日期：1996-07-01 00:00:00
abstract：:In many skeletal support systems of plants and animals, cellulose, chitin, and collagen occur in the form of microfibrils ordered in a chiral nematic fashion (helicoids). However, these structures remain poorly understood due to the many constituents present in biological tissues. Here we report an in vitro system tha...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(05)80008-x
更新日期：1992-06-01 00:00:00
abstract：:Polysialic acid (PSA), an acidic polysaccharide usually exists as a double-chain structure on cell adhesion molecules in vertebrates. The available PSA produced from Escherichia coli fermentation, however, is monochain PSA. In this work, a biomimetic biantenna type PSA (biPSA) was synthesized in vitro under mild condi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.11.107
更新日期：2020-07-15 00:00:00
abstract：:To evaluate the structural influence of the DNA phosphate backbone on the activity of Escherichia coli DNA topoisomerase I, modified forms of oligonucleotide dA(7) were synthesized with a chiral phosphorothioate replacing the non-bridging oxygens at each position along the backbone. A deoxy-iodo-uracil replaced the 5'...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(01)00165-9
更新日期：2001-10-22 00:00:00
abstract：:Wound healing is a complex, dynamic and difficult process. Much effort and attempt has been made to accelerate this process. The purpose of this study is to prepare nanoparticles loaded with vaccarin (VAC-NPS)hydrogel and evaluate its effect on promoting wound healing. In the present study, the physicochemical propert...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.10.182
更新日期：2020-12-15 00:00:00
abstract：:This paper reports synthesis of hydrogel from an unexplored Jhingan gum via microwave assisted technique for efficient remediation of toxic RBBR dye from aqueous solution. The synthesized hydrogel was confirmed through various analytical techniques. Adsorption mechanism was studied comprehensively and correlation coef...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.192
更新日期：2020-05-15 00:00:00
abstract：:Injectable and self-healing hydrogels have found numerous applications in drug delivery, tissue engineering and 3D cell culture. Herein, we report an injectable self-healing carboxymethyl chitosan (CMCh) supramolecular hydrogels cross-linked by zinc ions (Zn2+). Supramolecular hydrogels were obtained by simple additio...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.04.025
更新日期：2018-07-15 00:00:00
abstract：:Multienzymatic conversion of carbon dioxide (CO2) into chemicals has been extensively studied. However, regeneration and reuse of co-factor are still the main problems for the efficient conversion of CO2. In this study, a nanoscale multienzyme reactor was constructed by encapsulating simultaneously carbonic anhydrase ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.177
更新日期：2020-07-15 00:00:00
abstract：:Indian cobra (Naja naja) envenomation is frequently reported across Indian subcontinent. Geographical differences in the venom composition of a particular species of snake often leads to inconsistencies in the antivenom neutralization. Consequently, determining the venom proteome from every locale is necessary for the...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.05.106
更新日期：2020-10-01 00:00:00
abstract：:Here, we aims to demonstrate a simple concept in biomaterials design by using natural resources solely as raw materials to fabricate elastic wound care dressing. Elasto-gel films comprise of silk sericin (SRC), natural rubber (NR), and chitin whisker (CTW) were developed. A glue-like protein SRC found in silk cocoons ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.03.094
更新日期：2017-08-01 00:00:00
