Abstract:
:All hematopoietic cells that develop in the bone marrow must cross the endothelial barrier to enter the blood circulation. Blood platelets, however, are released by bigger protrusions of huge progenitor cells, named megakaryocytes, and enter the blood stream as so-called proplatelets before fragmenting into mature platelets. Recently, a second function of megakaryocytes has been identified, as they modulate the quiescence of hematopoietic stem cells, mostly via different soluble factors. We know from light sheet fluorescence microscopy images that megakaryocytes are distributed throughout the bone marrow facing a dense vascular network. Here, we used such three-dimensional images to provide a realistic simulation template reflecting the in vivo cell-vessel distributions resulting in reliable whole-bone analysis in silico Combining this approach with an automated image analysis pipeline, we found that megakaryocytes influence migration of neutrophils and hematopoietic stem cells, and thus act as biomechanical restrainers modulating cell mobility and extravasation. Indeed, as a consequence of increased megakaryocyte volumes in platelet-depleted mice neutrophil mobility was reduced in these animals.
journal_name
Haematologicajournal_title
Haematologicaauthors
Gorelashvili MG,Angay O,Hemmen K,Klaus V,Stegner D,Heinze KGdoi
10.3324/haematol.2018.202010subject
Has Abstractpub_date
2020-04-01 00:00:00pages
895-904issue
4eissn
0390-6078issn
1592-8721pii
haematol.2018.202010journal_volume
105pub_type
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