Performance evaluation of a line blot assay system for detection of anti-PM-Scl antibody in Japanese patients with systemic sclerosis.

Abstract:

OBJECTIVE:To evaluate the performance of a line blot (LB) assay for identifying anti-PM-Scl antibody (Ab). METHODS:We screened 56 serum samples from systemic sclerosis patients using a protein immunoprecipitation (IP) assay and an LB assay (Systemic Sclerosis Profile Euroline® Blot test kit) to detect anti-PM-Scl Ab. We compared the results obtained by the IP and LB assays. RESULTS:Among the 56 serum samples of SSc patients, 9 sera were positive for anti-PM-Scl75 Ab and 1 for anti-PM-Scl100 Ab by the LB assay. The protein IP assay revealed that none of the samples precipitated 75 or 100 kDa proteins identical to the anti-PM-Scl Ab reference serum, regardless of the positive or negative results obtained in the LB assay. Therefore, the false-positive rates for both anti-PM-Scl75 Ab and anti-PM-Scl100 Ab by the LB assay were 100%. CONCLUSION:Detection of anti-PM-Scl Ab assessed with a commercial LB assay requires extreme caution since it may yield false-positive data.

journal_name

Int J Rheum Dis

authors

Hamaguchi Y,Kuwana M,Takehara K

doi

10.1111/1756-185X.13638

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

1746-1751

issue

9

eissn

1756-1841

issn

1756-185X

journal_volume

22

pub_type

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