Abstract:
:Warfarin is the most worldwide used anticoagulant drug and rodenticide. Since it crosses placental barrier it can induce warfarin embryopathy (WE), a fetal mortality in neonates characterized by skeletal deformities in addition to brain hemorrhages. Although the effects of warfarin exposure in aquatic off target species were already described, the particular molecular toxicological mechanisms during early development are still unclear. Here, we used zebrafish (Danio rerio) to describe and compare the developmental effects of warfarin exposure (0, 15.13, 75.68 and 378.43 mM) on two distinct early developmental phases (embryos and eleuthero-embryos). Although exposure to both developmental phases induced fish mortality, only embryos exposed to the highest warfarin level exhibited features mimicking mammalian WE, e.g. high mortality, higher incidence of hemorrhages and altered skeletal development, among other effects. To gain insights into the toxic mechanisms underlying warfarin exposure, the transcriptome of embryos exposed to warfarin was explored through RNA-Seq and compared to that of control embryos. 766 differentially expressed (564 up- and 202 down-regulated) genes were identified. Gene Ontology analysis revealed particular cellular components (cytoplasm, extracellular matrix, lysosome and vacuole), biological processes (mainly amino acid and lipid metabolism and response to stimulus) and pathways (oxidative stress response and apoptosis signaling pathways) being significantly overrepresented in zebrafish embryos upon warfarin exposure. Protein-protein interaction further evidenced an altered redox system, blood coagulation and vasculogenesis, visual phototransduction and collagen formation upon warfarin exposure. The present study not only describes for the first time the WE in zebrafish, it provides new insights for a better risk assessment, and highlights the need for programming the rat eradication actions outside the fish spawning season to avoid an impact on off target fish community. The urge for the development of more species-specific anticoagulants for rodent pest control is also highlighted.
journal_name
Ecotoxicol Environ Safjournal_title
Ecotoxicology and environmental safetyauthors
Granadeiro L,Dirks RP,Ortiz-Delgado JB,Gavaia PJ,Sarasquete C,Laizé V,Cancela ML,Fernández Idoi
10.1016/j.ecoenv.2019.06.042subject
Has Abstractpub_date
2019-10-15 00:00:00pages
559-571eissn
0147-6513issn
1090-2414pii
S0147-6513(19)30680-3journal_volume
181pub_type
杂志文章abstract::Lipophilic persistent organic pollutants (POPs) are released from fat reserves during fasting, causing increased blood concentrations. Thus, POPs represent a potential anthropogenic stressor during fasting periods. We analysed the blood of female common eiders (Somateria mollissima) by using agarose gel electrophoresi...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2014.04.020
更新日期:2014-08-01 00:00:00
abstract::Lampricides are currently being applied to streams and rivers to control the population of sea lamprey, an invasive species, in the Great Lakes. The most commonly used lampricide agent used in the field is 3-trifluoromethyl-4-nitrophenol (TFM), which targets larval sea lamprey in lamprey-infested rivers and streams. T...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2018.07.017
更新日期:2018-10-30 00:00:00
abstract::Deltamethrin is a commonly used pyrethroid pesticide. Vitamin E is a antioxidant that plays an important role in protecting cells against toxicity by inactivating free radicals generated following pesticides exposure. Therefore, in the present study, it was evaluated whether deltamethrin induced changes on the fatty a...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2012.04.012
更新日期:2012-06-01 00:00:00
abstract::Three related amides (diuron, 2-(octyloxy) acetanilide, and salicylanilide) were evaluated for toxicity to aquatic microcosm communities. Effects were measured at the ecosystem level using changes in pH, Eh (redox potential), and dissolved oxygen as indicators of toxicity. These values were used to calculate the resis...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/0147-6513(87)90012-1
更新日期:1987-04-01 00:00:00
abstract::Brassica juncea (Indian mustard) L. plants were exposed to different concentrations (0.0, 0.1, 0.2 and 0.3mM) of arsenic (V) and harvested after 30 and 60 days of sowing for the analysis of growth parameters, metal uptake, brassinosteroids (BRs) synthesis and oxidative stress markers. As (V) significantly hampered the...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2015.02.016
更新日期:2015-05-01 00:00:00
abstract::The present study investigated estrogenic activity and total lipid levels in maternal serum and breast milk. The study was performed with 50 mothers from Fang district of northern Thailand. Maternal serum was collected 5 times, including the second trimester, pre-delivery period, delivery period, and lactating period ...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2009.08.023
更新日期:2010-05-01 00:00:00
abstract::Epidemiological studies have shown that high molecular weight phthalates (HMW) such as diethylhexyl phthalate (DEHP), are associated with hypertension in humans, while low molecular weight phthalates (LMW) such as dibutyl phthalate (DBP), have hardly any impact on the elevation of blood pressure. However, the molecula...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2019.02.067
更新日期:2019-06-15 00:00:00
abstract::This study explored the suitability of the seahorse Hippocampus reidi (Ginsburg, 1933) for assessing biomarkers of genotoxic effects and its use as a sentinel organism to detect the effects of acute exposure to petroleum hydrocarbons. Fish were exposed to three concentrations of crude oil (10, 20 and 30 g/kg) for 96 h...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2015.03.016
更新日期:2015-07-01 00:00:00
abstract::Triphenyl phosphate (TPP) has been found in various environmental media and in biota suggesting widespread human exposure. However, there is still insufficient information on the hepatotoxicity mechanisms of health risk exposed to TPP. In this study, TPP could induce human normal liver cell (L02) apoptosis, injury cel...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2020.111126
更新日期:2020-12-01 00:00:00
abstract::In a previous study a simple algorithm was presented for effect assessment on secondary poisoning of birds and mammals. This algorithm (MPC = NOECfish-eater/BCFfish) was drawn up by analyzing a two-step aquatic food chain (water-fish-bird/mammal). The algorithm was used to test whether quality criteria set for surface...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1006/eesa.1994.1011
更新日期:1994-03-01 00:00:00
abstract::Biochar has been recently used as an alternative strategy to improve soil quality and plant growth in metal contaminated soils. However, the effects of biochar on gas exchange parameters such photosynthetic rate (A), water use efficiency (WUE) and instantaneous carboxylation efficiency (ICE) in metal tolerant plant sp...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2019.109557
更新日期:2019-11-15 00:00:00
abstract::Human health and the health of the environment have entwined. In this paper we underpin this position by presenting a modeling approach named CombiSimilarity, which has been developed by the first author in the software tool PyHasse comprising a wide variety of partial ordering tools. A case study of 18 Organochlorine...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2014.03.031
更新日期:2014-07-01 00:00:00
abstract::Five random vegetable farms were selected to investigate the bioaccumulation risk of heavy metals (HMs) by different type of vegetables around the Pearl River Delta (PRD), China. The concentration order of four major HMs in the surface soil samples was Cd journal_title:Ecotoxicology and environmental safety pub_type: 杂志文章 doi:10.1016/j.ecoenv.2013.01.001 更新日期:2013-05-01 00:00:00
abstract::Chemical partitioning of heavy metals (Fe, Mn, Cu, Cr, Pb, Zn, Ni) were determined in surface sediments of three reservoirs at the Delta region of Cauvery River, India. The abundance of metals in sediments varied in the following descending order: Fe, Mn, Cr, Zn, Cu, Ni, and Pb. Higher concentrations of Zn, Pb, Fe, Mn...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2014.11.032
更新日期:2015-03-01 00:00:00
abstract::Nodularin (NODLN) is a hepatotoxin produced by the cyanobacterium Nodularia spumigena, which occurs regularly in the Baltic Sea. The primary aim of this study was to study the transfer of NODLN to three-spined stickleback (Gasterosteus aculeatus L.), herring (Clupea harengus membras L.), and salmon (Salmo salar L.), w...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2006.02.006
更新日期:2007-03-01 00:00:00
abstract::Sensitivities of a wildlife species, deer mice, to oxidants were evaluated. A single dose (1589 mg/kg body weight by intraperitoneal injection) of carbon tetrachloride, a typical hepatotoxicant, caused changes in GCL activity and GSH content in multiple organs of deer mice. Hepatic GCL activity and GSH content were de...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2009.02.008
更新日期:2009-07-01 00:00:00
abstract::Antimony (Sb) and its compounds are emerging priority pollutants which pose a serious threat to the environment. The aim of this study was to evaluate the short-term fate of antimonate added to different soils (S1 and S2) with respect to its mobility and impact on soil microbial communities and soil biochemical functi...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2020.110576
更新日期:2020-06-15 00:00:00
abstract::The lower tier toxicity tests used for risk assessment of plant protection products are conducted with single species, only regarding direct effects of the tested substances. However, it is not clear, if lower tier tests are able to protect in situ soil communities, as these tests are not able to account for direct an...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2019.109596
更新日期:2019-11-15 00:00:00
abstract::Sulfometuron-methyl is a broad-spectrum herbicide, used throughout Brazil; however, its environmental impacts in biochar (BC) amended soils is not fully understood. Biochar is known to enhance soil quality but can also have undesired effects such as altering the bioavailability and behavior of herbicides. Microbial co...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2020.111222
更新日期:2021-01-01 00:00:00
abstract::A rare sexual-only race of Dugesia dorotocephala produces offspring from cocoons rather than by fissioning, allowing individuals to be accurately aged. Because each animal possesses fully developed male and female reproductive systems and a true brain with synapses, many types of toxicological studies which normally u...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/0147-6513(89)90022-5
更新日期:1989-12-01 00:00:00
abstract::Arsenic (As) contamination of soil and water has been considered as a major global environmental issue during last few decades. Among the various methods so far reported for reclamation of As contaminated rhizosphere soil, bioremediation using bacteria has been found to be most promising. An As resistant bacterial iso...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2014.06.007
更新日期:2014-09-01 00:00:00
abstract::The concentrations of several organic contaminants (OCs) and heavy metals were measured in indoor dust from e-waste recycling, rural, and urban areas in South China to illustrate the spatial characteristics of these pollutants and to further evaluate human exposure risks. The median concentrations of polychlorinated b...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2017.02.041
更新日期:2017-06-01 00:00:00
abstract::The effects of decabromodiphenyl ether (BDE-209) on avoidance response, survival, growth, and reproduction of earthworms (Eisenia fetida) were investigated under laboratory conditions using natural and artificial soils as substrate. Results showed that no significant avoidance response was observed when earthworms wer...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2012.12.009
更新日期:2013-04-01 00:00:00
abstract::The structure and function of soil microbial communities have been widely used as indicators of soil quality and fertility. The effect of biochar application on carbon sequestration has been studied, but the effect on soil microbial functional diversity has received little attention. We evaluated effects of biochar ap...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2017.06.075
更新日期:2017-10-01 00:00:00
abstract::Metals and metalloids released through anthropogenic activities can accumulate in aquatic organisms, resulting in adverse effects in sensitive species. We investigated the influence of feeding regime and exposure complexity (i.e., mixture) on bioaccumulation kinetics and body distribution of common metal(loid) polluta...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2017.06.047
更新日期:2017-10-01 00:00:00
abstract::Dredging operations in harbours are recurrent to maintain accessibility and navigational depths. One of the main environmental risks of these operations is the remobilization of contaminants trapped in the sediments, rendering them more bioavailable to the biota. However, regulatory policies regarding the contaminatio...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2012.08.008
更新日期:2012-11-01 00:00:00
abstract::Effluent from tannery, electroplating and electronic industries contain chromium which is highly toxic. This paper reports isolation of chromium tolerant microorganisms from solid waste as well as liquid effluent of an electroplating industry. Nine isolates were obtained that can tolerate chromium concentration up to ...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2007.12.017
更新日期:2008-11-01 00:00:00
abstract::The potential adverse effects of e-waste recycling activity on environment are getting increasing concern. In this work, a model alga, Pseudokirchneriella subcapitata, was employed to assess the toxic effects of the leachates of paddy soils and river sediments collected from e-waste dismantling sites. Chemical analysi...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2014.10.012
更新日期:2015-01-01 00:00:00
abstract::Rare earth elements such as lanthanum (La) have been used as agricultural inputs in some countries in order to enhance yield and improve crop quality. However, little is known about the effect of La on the growth and structure of soybean, which is an important food and feed crop worldwide. In this study, bioaccumulati...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2015.07.020
更新日期:2015-12-01 00:00:00
abstract::The full understanding of the single and joint toxicity of a variety of organophosphorus (OP) pesticides is still unavailable, because of the extreme complex mechanism of action. This study established a systems-level approach based on systems toxicology to investigate OP pesticide toxicity by incorporating ADME/T pro...
journal_title:Ecotoxicology and environmental safety
pub_type: 杂志文章
doi:10.1016/j.ecoenv.2017.10.066
更新日期:2018-03-01 00:00:00