Antibacterial activity of prenylated benzopyrans from Peperomia obtusifolia (Piperaceae).

Abstract:

:Peperomia obtusifolia is a herbaceous perennial plant native to the Americas reported as a traditional medicine to treat snake bites and as a skin cleanser. The bioassay-guided fractionation of crude extracts from aerial parts of P. obtusifolia against a panel of clinically important fungi and bacteria, showed that hexane and dichloromethane extracts demonstrated selective bacterial inhibition, allowing the isolation of the known compounds peperobtusin A (1), and 3,4-dihydro-5-hydroxy-2,7-dimethyl-8-(3"-methyl-2"-butenyl)-2-(4'-methyl-1',3'-pentadienyl)-2H-1-benzopyran-6-carboxylic acid (2) from dichloromethane extract. Compound 2 was active against Gram-positive bacteria including community acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates and an Enterococcus faecalis vancomycin-resistant strain, with minimal inhibitory concentration (MIC) values of 4 μg/mL (10.8 μM) and 8 μg/mL (21.6 μM) respectively. The interaction of compound 2 with the bacterial membrane was demonstrated by means of Zeta potential experiments on S. aureus, then confirming the membrane damage by fluorescent microscopy experiments.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Ruiz Mostacero N,Castelli MV,Cutró AC,Hollmann A,Batista JM Jr,Furlan M,Valles J,Fulgueira CL,López SN

doi

10.1080/14786419.2019.1628751

subject

Has Abstract

pub_date

2019-06-14 00:00:00

pages

1-5

eissn

1478-6419

issn

1478-6427

pub_type

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