Abstract:
:This study aimed to investigate the mechanism underlying the inhibitory effect of tumor suppressor gene miR-186 and zinc finger protein 545 (ZNF545) on the proliferation of multiple myeloma (MM) cells. CD138 magnetic beads were used to isolate different types of myeloma cell lines (KM3, U266, RPMI-8226, and H929), which were then infected by lentivirus carrying the miR-186 gene. Using uninfected myeloma cells as the control, MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, Thiazolyl Blue Tetrazolium Bromide] assay was performed to calculate the rate of cell proliferation at different time points. In addition, the correlation between the expression of Jagged 1 and miR-186 was analyzed by real-time Polymerase Chain Reaction (PCR). Furthermore, the effect of 5-Aza-2-deoxycytidine and acetylase inhibitor Trichomycin A (TSA) on the expression of ZNF545 and proliferation/apoptosis of MM cells was investigated using Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Western blotting (WB), MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] cell proliferation assay, and Annexin V-FITC/PI staining. Compared with the control group, the proliferation of miR-186-overexpressing U266 and RPMI-8226 cells was significantly decreased. In cell cloning experiments, miR-186 decreased the number of U266 and RPMI-8226 clones while reducing the protein expression of Jagged 1. The expression level of ZNF545 in myeloma patients was also reduced to some extent. ZNF545 protein also promoted the apoptosis of myeloma cells. By inhibiting the proliferation of myeloma cells, miR-186 gene and ZNF protein may be used as tumor suppressors in the treatment of myeloma.
journal_name
J Biol Regul Homeost Agentsjournal_title
Journal of biological regulators and homeostatic agentsauthors
Deng S,Xiang JJ,Ge HP,Hu ZP,Shen JP,Lin SY,Zeng YQsubject
Has Abstractpub_date
2019-05-01 00:00:00pages
721-729issue
3eissn
0393-974Xissn
1724-6083pii
08journal_volume
33pub_type
杂志文章abstract::Over the past three years, most efforts of virologists and immunologists involved in the field of Human Immunodeficiency Virus (HIV) have been focussed on strategies aimed at eradicating the infection. So far, however, all the approaches have been unsuccessful. Indeed, even if the currently available potent antiretrov...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Williams Syndrome is a rare congenital disorder characterized by supravalvular aortic stenosis, peripheral pulmonary artery stenosis, mental retard and dysmorfic facial features. As regards the dental aspects of the syndrome, the deletion of the elastin gene induced clinicians to suspect periodontal alterations with a...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
abstract::Establishment of Leishmania infection inside macrophages requires deactivation of various signaling pathways that are dispensable for effective immune responses against the parasite. In the present study, we provide evidence that Leishmania infantum promastigotes attachment on the surface of peritoneal macrophages, in...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::This study was carried out by varying both graphene oxide (GO) concentration (10 μg/mL, 50 μg/mL, 100 μg/mL) and flakes sizes of 1320 nm and 130 nm. Characterization by scanning electron microscopy and Raman spectroscopy demonstrate that the area of GO flakes varies of one order of magnitude but their c...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00
abstract::Twinky Star colored compomer produced by VOCO GmbH is a new type of filling material designed with seven different colors. Previous studies have shown that using the colored filling material can induce childrens curiosity and increase the acceptance for dental treatment. However, few studies have been conducted to ev...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
abstract::Long non-coding ribonucleic acids (lncRNAs) have been recognized as markers in several cancers and play important roles in glioblastoma (GBM). But the role of lncRNA X inactive-specific transcript (XIST) in GBM and its possible mechanisms are rarely studied in depth. This study was conducted to explore the detailed ro...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:10.23812/20-558-L
更新日期:2020-11-01 00:00:00
abstract::Adhesive capsulitis (AC) is a common pathological condition of the shoulder characterized by painful restriction of range of motion (ROM) of the glenohumeral joint. Currently, no consensus has been reached regarding the best treatment. Hyaluronic acid (HA) injection is a safe procedure that can result in significant i...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-12-27 00:00:00
abstract::To study the effect and related mechanism of IL-21 alone and in combination with 5-Fluorouracil on the proliferation and growth, transferability, and apoptosis of gastric cancer cells, we cultivated gastric cancer cell SGC-7901 and created four experimental groups with varying concentrations of IL-21 and 5-Fluorouraci...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2018-05-01 00:00:00
abstract::Throughout the centuries, the undefended nature of man has very often manifested in cases of epidemics, which have determined scenarios of suffering and death. Through “historical” observation lens, it is possible to understand the relationship between the spread of viruses, bacteria and parasites responsible for thes...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
abstract::Acute severe respiratory syndrome coronavirus-2 (SARS-CoV-2) caused a global pandemic coronavirus disease 2019 (COVID-19). In humans, SARS-CoV-2 infection leads to acute respiratory distress syndrome which presents edema, hemorrhage, intra-alveolar fibrin deposition, and vascular changes characterized by thrombus form...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论
doi:10.23812/EDITORIAL-RONCONI-E-59
更新日期:2020-05-01 00:00:00
abstract::Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells (HPC) is widely used for evaluation of graft adequacy of peripheral blood stem cell grafts, and is also useful in planning the apheresis sessions necessary to obtain these grafts. The state-of-the-art method to enumerate CD34+ cells makes use...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2001-01-01 00:00:00
abstract::In the aesthetic field, successful replacement of a tooth with a dental implant requires blend and harmony within the existing dentition. The influence of the dimension of buccal bone at implant sites on aesthetic outcomes and the relation between buccal bone horizontal and vertical dimensions are unclear. The aim of ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
abstract::Notch signaling plays a critical role in the development and function of macrophages. The aim of the present study was to investigate the relationship between Notch signaling pathway and macrophage apoptosis after LPS stimulation. In RAW 264.7 cells, the mRNA expression of Jagged1, Hes1, Hes 5 and GM-CSF, and protein ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:10.23812/19-283-A
更新日期:2020-02-12 00:00:00
abstract::As the incidence of ischemic cerebrovascular disease increases continuously over the years, carotid atherosclerosis as an important dangerous factor has drawn a lot of attention from many experts and scholars. To explore the clinical significance of high resolution magnetic resonance angiography (MRA) in carotid ather...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2015-04-01 00:00:00
abstract::There is wide discussion about the association between sport activity and musculoskeletal disorders, as sports-related joint loading increases the risk of osteoarthritis (OA). The present article reviews the current available literature on the connections between participation in several sports and athletic activities...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2019-03-01 00:00:00
abstract::Direct anterior approach to the hip allows perfect exposure of the acetabulum and an easy proximal and medial extension that makes it eligible for isolate acetabular cup revision although it is seldom used and there are only few published studies. On 23 consecutive acetabular revision (16 cases Paprosky grade 1 or 2, ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2016-10-01 00:00:00
abstract::This study is to compare the cleaning effectiveness of two Ni-Ti files systems. Thirty single-rooted human teeth were selected and two NiTi rotary systems were used. Group A: canal shaping with ProTaper® Universal (Dentsply Tulsa Dental Specialties, Tulsa, OK) (PTU); Group B: (n=15) canal shaping with Mtwo Ni-Ti instr...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2019-05-01 00:00:00
abstract::Ahead of Print article withdrawn by publisher. ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论,收录出版
doi:10.23812/20-269-E-4
更新日期:2020-07-16 00:00:00
abstract::Four new complexes of Hg (II), Pb (II), Sn (II) and Bi (III) with indomethacin drug ligand (IMC) were synthesized and characterized by using infrared, electronic, 1H-NMR spectral, thermogravimetric and conductivity measurements. The IMC was found to act as bidentate chelating agent. IMC complexes coordinate through th...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2015-07-01 00:00:00
abstract::The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DR alpha gene was studied. As the HLA-DR alpha gene is inducible by interferon(IFN)-gamma, nuclear extracts were also purified from IFN-gamma treated cells. Our data indicate that ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::The early diagnosis and treatment of individuals harboring M. tuberculosis is key to ensuring the effectiveness of health programs aimed at the elimination of tuberculosis (TB). Monitoring for TB also has other important health care implications for the related immune pathology caused by the chronic inflammatory respo...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2011-04-01 00:00:00
abstract::The Risk of Malignancy Algorithm (ROMA) combines the diagnostic power of the CA125 and HE4 markers with menopausal status to predict the risk for developing epithelial ovarian cancer (EOC). The aim of this study was to evaluate the association between 25-OH vitamin D levels and ROMA score in obese women. One hundred...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2016-10-01 00:00:00
abstract::Th17 cells are a new T-cell subtype characterized by the capability of producing IL-17. They are reported to be involved in a wide range of cutaneous immune-mediated conditions and, particularly in this review, we sought to elucidate the Th17 role in the pathogenesis of some common inflammatory diseases including psor...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论,评审
doi:
更新日期:2012-07-01 00:00:00
abstract:: ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2019-01-01 00:00:00
abstract::The aim of this work was to study the effects of micro ribonucleic acid (miR)-20a on acute kidney injury (AKI) in sepsis rats and its key molecular mechanism. Sprague-Dawley rats were randomly divided into healthy rat group (H group, n=3), sham group (S group, n=3), sepsis rat group (D group, n=3), sepsis rat + negati...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:10.23812/20-174-A
更新日期:2020-07-01 00:00:00
abstract::HAART is the cornerstone of HIV therapy, and has significantly reduced morbidity and mortality associated with HIV disease. The institution of HAART during the primary HIV-1 infection has a more profound influence on the ultimate pattern and rate of disease progression than therapy commenced later on. However, it also...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::We tried to identify molecular markers in peripheral blood to predict high risk of aneurysm rupture. Extraction of the total population of peripheral blood mononuclear cell (PBMC) from total blood volume, total RNA extraction from PBMC and Agilent One Color Gene-expression Oligo-Microarray were performed on peripheral...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2013-07-01 00:00:00
abstract::The aim of the study was to explore the effect of lipoxin A4 (LXA4) on lung injury in sepsis rats through the p38/mitogen-activated protein kinase (MAPK) signaling pathway. Sprague-Dawley rats were used for the study. The rat model of sepsis-induced acute lung injury was established via cecal ligation (Sepsis group, n...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:10.23812/20-108-A-20
更新日期:2020-05-01 00:00:00
abstract::Pubic osteolysis is a rare pathology characterized by a painful radiographic destructive changes in the pubic rami, pubis or pubic symphysis that often follows a post-traumatic event. The etiology is unclear but it is a benign lesion, frequently misinterpreted as malignant. We report a case of a 54-year-old woman with...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2019-03-01 00:00:00
abstract::To compare the effectiveness of Doctor Tecar TherapyTM with that of laser therapy in the management of low back pain (LBP), a total of 60 patients with LBP were enrolled. The participants were randomly divided into two groups: a Tecar group (experimental group, 30 subjects), and a laser group (control group, 30 subjec...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2017-04-01 00:00:00