Abstract:
:Cell biology is increasingly dependent on quantitative methods resulting in the need for microscopic labelling technologies that are highly sensitive and specific. Whilst the use of fluorescent proteins has led to major advances, they also suffer from their relatively low brightness and photo-stability, making the detection of very low abundance proteins using fluorescent protein-based methods challenging. Here, we characterize the use of the self-labelling protein tag called HaloTag, in conjunction with an organic fluorescent dye, to label and accurately count endogenous proteins present in very low numbers (<7) in individual Escherichia coli cells. This procedure can be used to detect single molecules in fixed cells with conventional epifluorescence illumination and a standard microscope. We show that the detection efficiency of proteins labelled with the HaloTag is ≥80%, which is on par or better than previous techniques. Therefore, this method offers a simple and attractive alternative to current procedures to detect low abundance molecules.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Lepore A,Taylor H,Landgraf D,Okumus B,Jaramillo-Riveri S,McLaren L,Bakshi S,Paulsson J,Karoui MEdoi
10.1038/s41598-019-44278-0subject
Has Abstractpub_date
2019-05-27 00:00:00pages
7902issue
1issn
2045-2322pii
10.1038/s41598-019-44278-0journal_volume
9pub_type
杂志文章abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-42527-w
更新日期:2019-04-12 00:00:00
abstract::Allergic contact hypersensitivity (CHS) is an inflammatory skin disease mediated by allergen specific T cells. In this study, we investigated the role of transcription factor NFAT1 in the pathogenesis of contact hypersensitivity. NFAT1 knock out (KO) mice spontaneously developed CHS-like skin inflammation in old age. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19453
更新日期:2016-01-18 00:00:00
abstract::Fundamental life history processes of mycorrhizal fungi with inconspicuous fruiting bodies can be difficult to elucidate. In this study we investigated the species identities and life history of the orchid mycorrhizal Tulasnella fungi, which associate with the south eastern Australia orchid genus Chiloglottis. Tulasne...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05855-3
更新日期:2017-07-17 00:00:00
abstract::Accurate intraocular lens (IOL) power calculation is always a challenge in ophthalmology, and unoptimized process may lead to inaccurate refractive outcomes. Quality control circle (QCC) has shown its success in many fields as a process management tool. However, its efficacy in ophthalmology remains unclear. Here we u...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14171-9
更新日期:2017-11-01 00:00:00
abstract::This study describes a new two-step process to cool the thermal vibration of microcantilevers. The process combines active mechanical feedback cooling and optical cavity cooling. A micro-Fabry-Perot interferometer, built in-house, is set atop a microcantilever to measure the vibration amplitude, the high optical power...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55496-x
更新日期:2019-12-13 00:00:00
abstract::Early diagnosis of hepatocellular carcinoma (HCC) remains challenging to date. Characteristic metabolic deregulations of HCC may enable novel biomarkers discovery for early diagnosis. A capillary electrophoresis-time of flight mass spectrometry (CE-TOF/MS)-based metabolomics approach was performed to discover and vali...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16101
更新日期:2015-11-03 00:00:00
abstract::Transplantation of stem cell-derived insulin producing cells (IPCs) has been proposed as an alternative to islet transplantation for the treatment of diabetes mellitus. However, current IPC differentiation protocols are focused on generating functional cells from the pluripotent stem cells and tend to rely on multiste...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41823-9
更新日期:2019-03-29 00:00:00
abstract::The recent surge in research on metal-halide-perovskite solar cells has led to a seven-fold increase of efficiency, from ~3% in early devices to over 22% in research prototypes. Oft-cited reasons for this increase are: (i) a carrier diffusion length reaching hundreds of microns; (ii) a low exciton binding energy; and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36108
更新日期:2016-11-08 00:00:00
abstract::The intravenous, intramuscular or intraperitoneal administration of water solubilized graphene nanoparticles for biomedical applications will result in their interaction with the hematological components and vasculature. Herein, we have investigated the effects of dextran functionalized graphene nanoplatelets (GNP-Dex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02584
更新日期:2013-01-01 00:00:00
abstract::Several lines of evidence indicate that Fibronectin Extra Domain A (EDA) promotes metastatic capacity of tumor cells by engaging cell surface α9β1 integrins. This interaction mediated by the C-C loop of EDA activates pro-oncogenic signaling pathways leading to epithelial to mesenchymal transition (EMT) of tumor cells,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37151
更新日期:2016-11-16 00:00:00
abstract::Envelope (E) and temporal fine structure (TFS) are important features of acoustic signals and their corresponding perceptual function has been investigated with various listening tasks. To further understand the underlying neural processing of TFS, experiments in humans and animals were conducted to demonstrate the ef...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12975-3
更新日期:2017-10-17 00:00:00
abstract::The early diverged Magnoliaceae shows a historical temperate-tropical distribution among lineages indicating divergent evolution, yet which ecophysiological traits are phylogenetically conserved, and whether these traits are involved in correlated evolution remain unclear. Integrating phylogeny and 20 ecophysiological...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12246
更新日期:2015-07-16 00:00:00
abstract::Human papillomavirus (HPV) infection, a primary cause of genital cancer, is also related to the increasing incidence of oropharyngeal cancer among young men. Relatively little is known about the concurrence of oral and genital infection among healthy individuals. Oral and genital swab exfoliated cells were collected s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15637
更新日期:2015-10-27 00:00:00
abstract::Antagonist pleiotropy, where a gene exerts a beneficial effect at early stages and a deleterious effect later on in an animal's life, may explain the evolutionary persistence of devastating genetic diseases such as Huntington's disease (HD). To date, however, there is little direct experimental evidence to support thi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37102-8
更新日期:2019-01-10 00:00:00
abstract::River ecosystem health assessments provide the foundation for river ecological protection and integrated management. To evaluate the aquatic ecosystem health of the Fan River basin, benthic macroinvertebrate indices (the Multimeric Macroinvertebrates Index Flanders (MMIF) and Family Biotic Index (FBI)), a habitat inde...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50499-0
更新日期:2019-10-01 00:00:00
abstract::Chaotic time series prediction based on nonlinear systems showed a superior performance in prediction field. We studied prenatal exposure to polychlorinated biphenyls (PCBs) by chaotic time series prediction using the least squares self-exciting threshold autoregressive (SEATR) model in umbilical cord blood in an elec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25005
更新日期:2016-04-27 00:00:00
abstract::Owing to their unique optical properties such as large absorption and scattering cross section and large enhancement of electromagnetic field at the surface, plasmonic nanostructures have received extensive attention as a highly promising class of materials for nano-oncology. Most of the existing plasmonic nanostructu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10311
更新日期:2015-05-14 00:00:00
abstract::Full-endoscopic transforaminal lumbar discectomy (FETD) is increasingly used in patients with lumbar disc herniation (LDH). There is little knowledge on the related factors, including the straight leg raising test (SLR), that influence the operation. Consecutive patients with LDH who came to our hospital from August 2...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73357-w
更新日期:2020-10-05 00:00:00
abstract::Ribosomal RNA internal transcribed spacer-1 (ITS1) metabarcoding was used to investigate the distribution patterns of fungal communities and the factors influencing these patterns in subtropical Chinese seas, including the southern and northern Yellow Sea and the Bohai Sea. These seas were found to harbor high levels ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26528
更新日期:2016-05-20 00:00:00
abstract::INK4 locus at chromosome 9p21 has been reported to be associated with primary open angle glaucoma (POAG) and its subtypes along with the associated optic disc parameters across the populations of European, Japanese and African ancestries. The locus encodes three tumor suppressor genes namely CDKN2A, ARF, CDKN2B and a ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05115
更新日期:2014-05-30 00:00:00
abstract::Growing evidence indicates that nomogram combined with the biomarkers of systemic inflammation response could provide more accurate prediction than conventional staging systems in tumors. This study aimed to establish an effective prognostic nomogram for resectable thoracic esophageal squamous cell carcinoma (ESCC) ba...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18811
更新日期:2015-12-22 00:00:00
abstract::Inter-specific emotion recognition is especially adaptive when species spend a long time in close association, like dogs and humans. Here, we comprehensively studied the human ability to recognize facial expressions associated with dog emotions (hereafter, emotions). Participants were presented with pictures of dogs, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52938-4
更新日期:2019-11-11 00:00:00
abstract::With Motor imagery (MI), movements are mentally rehearsed without overt actions; this procedure has been adopted in motor rehabilitation, primarily in brain-damaged patients. Here we rather tested the clinical potentials of MI in purely orthopaedic patients who, by definition, should maximally benefit of mental exerci...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65820-5
更新日期:2020-06-02 00:00:00
abstract::Hbs1, which is homologous to the GTPase eRF3, is a small G protein implicated in mRNA quality control. It interacts with a translation-release factor 1-like protein Dom34/Pelota to direct decay of mRNAs with ribosomal stalls. Although both proteins are evolutionarily conserved in eukaryotes, the biological function of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39530-6
更新日期:2019-03-01 00:00:00
abstract::The increasing availability of electronic health care records has provided remarkable progress in the field of population health. In particular the identification of disease risk factors has flourished under the surge of available data. Researchers can now access patient data across a broad range of demographics and g...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30465
更新日期:2016-07-27 00:00:00
abstract::The placenta has emerged as an attractive source of mesenchymal stem cells (MSCs) because of the absence of ethical issues, non-invasive access, and abundant yield. However, inflammatory cell invasion into grafts negatively impacts the survival and efficacy of transplanted cells. Previous studies have shown that synth...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22867-9
更新日期:2018-03-15 00:00:00
abstract::The relationship between obstructive sleep apnea (OSA) and chronic rhinosinusitis (CRS) still remains unclear. This retrospective cohort study aimed to investigate the relationship between OSA and subsequent CRS using a population-based dataset. The study used data from the Taiwan Longitudinal Health Insurance Databas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20786
更新日期:2016-02-10 00:00:00
abstract::Nutrient inputs and ultraviolet radiation (UVR) are global factors affecting the structure and functioning of aquatic ecosystems, particularly clear-water ecosystems. We performed experiments in two model lakes highly exposed to UVR fluxes in order to test the effect that future increases in mineral nutrients transpor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06279-9
更新日期:2017-07-24 00:00:00
abstract::Receptor-mediated endocytosis is an essential process in signalling pathways for activation of intracellular signalling cascades. One example is the Wnt signalling pathway that seems to depend on endocytosis of the ligand-receptor complex for initiation of Wnt signal transduction. To date, the roles of different endoc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49082-4
更新日期:2019-09-10 00:00:00
abstract::With the development of ultra-intense laser technology, MeV ions can be obtained from laser-foil interactions in the laboratory. These energetic ion beams can be applied in fast ignition for inertial confinement fusion, medical therapy, and proton imaging. However, these ions are mainly accelerated in the laser propag...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08274
更新日期:2015-02-05 00:00:00