Abstract：
:Nanothermometry methods with intracellular sensitivities have the potential to make important contributions to fundamental cell biology and medical fields, as temperature is a relevant physical parameter for molecular reactions to occur inside the cells and changes of local temperature are well identified therapeutic strategies. Here we show how the GFP can be used to assess temperature-based on a novel fluorescence peak fraction method. Further, we use standard GFP transfection reagents to assess temperature intracellularly in HeLa cells expressing GFP in the mitochondria. High thermal resolution and sensitivity of around 0.26% °C-1 and 2.5% °C-1, were achieved for wt-GFP in solution and emGFP-Mito within the cell, respectively. We demonstrate that the GFP-based nanothermometer is suited to directly follow the temperature changes induced by a chemical uncoupler reagent that acts on the mitochondria. The spatial resolution allows distinguishing local heating variations within the different cellular compartments. Our discovery may lead to establishing intracellular nanothermometry as a standard method applicable to the wide range of live cells able to express GFP.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Savchuk OA,Silvestre OF,Adão RMR,Nieder JBdoi
10.1038/s41598-019-44023-7subject
Has Abstractpub_date
2019-05-17 00:00:00pages
7535issue
1issn
2045-2322pii
10.1038/s41598-019-44023-7journal_volume
9pub_type
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