Satellitome landscape analysis of Megaleporinus macrocephalus (Teleostei, Anostomidae) reveals intense accumulation of satellite sequences on the heteromorphic sex chromosome.

Abstract:

:The accumulation of repetitive DNA sequences on the sex-limited W or Y chromosomes is a well-known process that is likely triggered by the suppression of recombination between the sex chromosomes, which leads to major differences in their sizes and genetic content. Here, we report an analysis conducted on the satellitome of Megaleporinus macrocephalus that focuses specifically on the satDNAs that have been shown to have higher abundances in females and are putatively located on the W chromosome in this species. We characterized 164 satellite families in M. macrocephalus, which is, by far, the most satellite-rich species discovered to date. Subsequently, we mapped 30 satellites, 22 of which were located on the W chromosome, and 14 were shown to exist only on the W chromosome. Finally, we report two simple, quick and reliable methods that can be used for sex identification in M. macrocephalus individuals using fin clips or scales, which could be applicable to future studies conducted in the field of aquaculture.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Utsunomia R,Silva DMZA,Ruiz-Ruano FJ,Goes CAG,Melo S,Ramos LP,Oliveira C,Porto-Foresti F,Foresti F,Hashimoto DT

doi

10.1038/s41598-019-42383-8

subject

Has Abstract

pub_date

2019-04-10 00:00:00

pages

5856

issue

1

issn

2045-2322

pii

10.1038/s41598-019-42383-8

journal_volume

9

pub_type

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